465 results about "Sulfate-reducing bacteria" patented technology

An apparatus and method produces nitrate ions on-site from water, natural gas and air extracted in proximity to the apparatus. The apparatus generates nitrate ions and brings the nitrate ions into contact with an aqueous system. Hydrogen sulfide present in the aqueous system is removed and the production of hydrogen sulfide by sulfate-reducing bacteria (SRB) is eliminated by introducing into the system nitrate ions, whereby denitrifying microorganisms, using nitrate, outcompete the sulfate-reducing bacteria for the available carbon nutrients, thus preventing the SRB from producing hydrogen sulfide. Nitrate ions generated by the apparatus and added to the aqueous system which contains the denitrifying microorganisms can enhance oil recovery by means of microbial enhanced oil recovery mechanisms.

A method of repairing mine ecological environment comprises the treatment of mine environment contamination, wherein the tailings, the barren rock, the smelting slag, the waste water from ore cleaning, the waste water from smelting, the acid mine drainage and the ore yard leachwater which are generated from mine development are collected into the tailings storage, at the same time, sludge and organic matter capable of being degradated by microbiology are added therein, and then the tailings storage is given an anaerobic environment artificially, so that, under the action of microbiology and sulfate reducing bacteria, sulfur ion is generated and the pH value of the tailings storage is increased, a plurality of heavy metal ions are cured and settled by sulfur ion to avoid migration, a biomembrane layer-sulfate reducing bacteria reducing-induction biomineralization precipitation composite geochemical barrier is generated in the tailings storage. The repairing of mine ecological environment is realized by the treatment of mine environment contamination. The present invention can be used for tailings storage pollution prevention in tour, and can be used for closing the repairing of mine ecological environment, or can be used for designing new tailings storage by using the technology of the present invention.

It has been discovered that certain fracturing fluid compositions can be used to fracture a subterranean formation and be permitted to stay within the formation for a relatively extended period of time, for example 28 days or more, before being flowed back or produced. At least two embodiments are envisioned, a bacteria-containing formulation and an anti-bacterial formulation. Both systems would be expected to prevent the potential of the fluid to oil wet the formulation (water block condition) by keeping the formation water wet through the use of water wetting surfactants or solvents. Additionally, both formulations would control reservoir crude souring (H₂S generation by in situ sulfate-reducing bacteria), reservoir plugging (via slime biopolymers generated by in situ microbes, inorganic scale deposition like calcium carbonate or barium sulfate, and clay fines migration).

The invention discloses a method for treating acidic waste water containing heavy metal and reclaiming the heavy metal through a biomass fixed bed, which is characterized in that dry solid biomass capable of being decomposed by microbes is granulated and filled into a container with a supporting layer and a water distributing device so as to form a fixed bed microbe reactor; sewage disposal plant sludge containing sulfate reducing bacteria is taken as the source of microbe bacteria on the fixed bed, and the microbes are cultured, proliferated and acclimated to adapt to the environment of the biomass filler fixed bed through the backflow circulation of the outputted water or taking ferrous sulfate aqueous solution as the inputted water; acid effluent containing heavy metallic ions is led into the fixed bed for direct filtration; and when the water filtering speed can not meet the preset requirements, the operation is stopped, substances in the fixed bed are taken out, the heavy metal is reclaimed through smelting, and the fixed bed microbe reactor is refilled. The method is suitable for waste water containing Cr2O7 <2->, AsO3 <2->, Cu <2+>, Hg <2+> Ag <+>, Cd <2+> and the like. The method can be used for reclaiming the valuable metal resources with low waste water treatment cost.

The invention discloses a bacterial strain b capable of resisting the growth of heavy metal Cd and reducing sulfate, and a bacterial strain capable of resisting the growth of the heavy metal Cd and solubilizing phosphate. The classification and name of the bacterial strain b and the bacterial strain are respectively enterbacter ludwigii SRB-2-5U-2 and pseudochrobactrum saccharolyticum LB-4-4-1c, which are already preserved in the Common Microbe Center of the China Committee for Culture Collection of Microorganisms. The bacterial strains disclosed by the invention can be used for simultaneously remediating the surface soil, deep soil and rhizosphere soil of a plant, reducing the pollution of Cd to surface water and underground water, and also improving the activity and fertility of the soil.

Biogenic sulfide production is synergistically inhibited by treating sulfate-reducing bacteria (SRB) with a biocide and a metabolic inhibitor. The biocide directly kills a first portion of the SRB. The metabolic inhibitor inhibits sulfate-reducing growth of a second portion of the SRB without directly killing the second portion of the SRB. The treatment of SRB with both a biocide and a metabolic inhibitor provides effective biogenic sulfide inhibition at significantly lower concentrations than would be required if the biocide or metabolic inhibitor was used alone.

The present invention belongs to the waste water treating technology utilizing sulfate reducing bacteria. Upwards flowing anaerobic composite bed bioreactor and mixed sulfate reducing bacteria are used in treating acid waste water containing Cr2O72-, CrO42-, Cu2+, Zn 2+, Mn2+ and other heavy metal ions. The present invention has high activity of sulfate reducing bacteria and high waste water treating efficiency. The present invention features that inside the reactor or waste water tank, simple substance iron is added to result in waste water pH value of 5.5-8.5, and that the biochemical reaction is performed at 25-30 deg.c. The present invention has the advantages of addition of simple substance iron, raised waste water pH value, improved bacteria living environment, high metabolic activity of the bacteria, reinforced capacity of the sulfate reducing bacteria, raised bacteria adaptability and waste water treating capacity.

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g/L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.

The invention belongs to the technical field of treatment of waste water, and particularly relates to a method for reduction of sulfate into sulfide and treatment of metal waste water by sulfate reducing bacteria growing up with electric current. The sulfate reducing bacteria provided by the invention is a mixing bacteria group, which comprises Desulfobulbus propinonicus, geobacter sulfurreducens. Under the condition of lacking organic carbon resource, an electrode is used as the only electronic donator for growing, sulfate is reduced into sulfide via bioelectrical chemistry, and the sulfide is applied to the treatment of metal waste water. According to the method provided by the invention, the defect that organic carbon resource is needed to be used as the electronic donator is overcome,the operation is simple, the secondary pollution is zero, the cost is low, and is particularly suitable for the treatment on organic carbon metal waste water of mine waste water, metal smelting wastewater, electric plating waste water and the like.

The present invention relates to one kind of functional biological carbon fiber and its preparation process and application as biomembrane carrier. The biomembrane carrier is prepared with polyacrylonitrile carbon fiber, and through electrochemical surface treatment in 5-20 % concentration ammonium bicarbonate electrolyte to increase the surface acid oxygen containing functional groups and raise wettability, and gluing with water soluble epoxy resin in the amount 0.5-2 %. The biomembrane carrier can fix nitrobacteria, sulfate reducing bacteria and anaerobic methanogen. It may be used in treating high concentration acrylon waste water, and can eliminate sulfate, COD and ammonia nitrogen effectively. In addition, the biomembrane carrier is easy to regenerate and has no secondary pollution.

The invention provides a method for biological treatment of sulfate wastewater employing synchronous electric catalysis of an anode and a cathode. The method comprises the following concrete steps of: (1) building an electric catalytic bioreactor, which includes a cathode reaction zone, a buffer zone and an anode reaction zone; (2) inoculating mixed bacteria of sulfate reducing bacteria inside the cathode reaction zone, leading to a culture medium, electrifying and acclimatizing, and cultivating for 3-5 days at 28-34 DEG C, so as to achieve cathode hanging membrane of the sulfate reducing bacteria; (3) inoculating sulfur-oxidizing bacteria inside the anode reaction zone, leading to the culture medium to cultivate for 5-7 days at 25-30 DEG C; and (4) starting a reactor, leading the sulfate wastewater to the cathode reaction zone of the reactor, and enabling the sulfate wastewater to flow out of the reactor after flowing inside the anode reaction zone through the buffer zone. By adopting the method, control of an S<2->oxidization process is achieved when the reduction speed of the sulfate reducing bacteria for reducing SO4<-2> is improved. Thus, the yield of S produced by biological metabolism of the wastewater is improved.

The invention discloses an oil field sewage bactericide and a preparation method thereof, belonging to the technical field of chemical agents for oil fields and preparation thereof. The oil field sewage bactericide is prepared by mixing the following components in percentage by mass: 10-15% of nano titanium dioxide powder, 0.5-2% of crosslinking agent, 5-10% of surfactant, 5-10% of oxidizer and the balance of water. The bactericide can be deposited on pipe walls, bent angles and other parts along with a liquid flow; a main agent can be slowly diluted out, has persistent chemical action and causes no corrosion to pipelines; the comprehensive sterilizing rate of the bactericide with respect to sulfate reducing bacteria, saprophytic bacteria and iron bacteria can be still up to 90% or above 18 days after filling; and the filling period of the chemical agent is 15-25 days which is 10-15 times longer in comparison with a common chemical agent, thereby greatly relieving the operation intensity and lowering the cost.

The invention belongs to the technical field of water treatment, and particularly relates to a sterilizing stripper dedicated for oilfield injection water and a preparation method thereof. The sterilizing stripper dedicated for the oilfield injection water is characterized by compounding the following materials in percentage by weight: 15 to 35 percent of tetrakis hydroxymetyl phosphonium sulfuric, 10 to 35 percent of dodecyl dimethyl benzyl ammonium chloride, 0.5 to 1.0 percent of sodium dioctylsulfosuccinate, and the balance of deionized water. The preparation method comprises the followingsteps: adding the deionized water into a reaction kettle; and adding the tetrakis hydroxymetyl phosphonium sulfuric, the dodecyl dimethyl benzyl ammonium chloride and the sodium dioctylsulfosuccinate, and stirring for 40 to 50 minutes at normal temperature. By the method, the stripping performance of the sterilizing stripper and the killing performance to bacteria, particularly sulfate reducing bacteria, are improved; the sterilizing stripper has strong sterilization capability, good penetration and remarkable stripping effect; and the preparation process is simple and easy to operate.

A method for the ecological regulation and control of the activity of aquatic sulfate reducing bacteria on oil field ground and a depressor used relate to a method for regulating and controlling the activity of sulfate reducing bacteria and a depressor used. The method solves the problems of the prior method for controlling SRB in an oil field including low sterilization efficiency and various disadvantages of bactericide. The depressor for inhibiting the activity of aquatic sulfate reducing bacteria on oil field ground is made from a denitrifying nitrifying bacteria liquid, nutritive element and a co-inhibiting substance in portion by mass. The method for the ecological regulation and control comprises the following steps that: (1) the depressor is prepared; and (2) the depressor is added according to the molar ratio between SO4<2-> and nitrate radical. The depressor has high efficiency in inhibiting sulfate reducing bacteria and avoids using a bactericide, thereby having high safety and environmental protection.

The invention relates to a method of using native function microflora to in-situ purify ground water polluted by uranium. The method translates transferable hexavalent uranium into nontransferable uranous uranium in the ground water through nitrate reducing bacteria flora, ferric iron reducing bacteria flora and sulfate reducing bacteria flora which are formed under the anaerobic condition by microorganism, and therefore the purpose of purifying the ground water polluted by the uranium is achieved. The specific implementation method comprises: (1) adjusting a potential of hydrogen (Ph) value of the ground water polluted by the uranium; (2) activating the microorganism in sediments; (3) regulating and controlling variation of the microflora, accelerating evolution of the nitrate reducing bacteria flora, the ferric iron reducing bacteria flora and the sulfate reducing bacteria flora by means of adding a carbon source, regulating oxidation reduction potential of the water polluted by the uranium and the like; (4) continuously regulating and controlling the microbial flora until uranium concentration in the ground water polluted by the uranium is reduced below the national emission standard and the purpose of purifying the groundwater polluted by the uranium is achieved. The method of using the native function microflora to in-situ purify the ground water polluted by the uranium solves in-situ purifying problem of the ground water polluted by the uranium, and has the advantages of being high in purifying efficient, short in period, simple in equipment, short in process procedure, low in purifying cost, strong in operability, free of secondary pollution, good in purifying effect and the like.

The invention relates to a method for biologically inhibiting the production of secondary hydrogen sulfide from sulfate reducing bacteria in a crude oil gathering and transporting system. In the method, (1) a biological inhibitor for inhibiting a single well consists of 50 mg/L sodium nitrite and 20 mg/L sodium borate; (2) the biological inhibitor for inhibiting an oil transfer station consists of 40 to 60 mg/L sodium nitrite, 25 milligrams of humic acid, 10 to 20 mg/L sodium borate and 20 to 30 mg/L sodium hydroxide electrolyte; and (3) the biological inhibitor for inhibiting a combined station consists of 40 to 60 mg/L sodium nitrite, 25 milligrams of humic acid, 10 to 20 mg/L sodium borate, 20 to 30 mg/L sodium hydroxide electrolyte and 95 mg/L glutaraldehyde bactericide. In the method, the generation of the hydrogen sulfide is controlled at the source, and the method has the characteristics of long hydrogen sulfide inhibition time, low cost and no secondary pollutions and improvesthe working environment of a workplace of the crude oil gathering and transporting system.

The invention discloses sulfate reducing bacteria with tolerance on arsenic. The sulfate reducing bacteria are named as enterobacteria (enterobacter sp.) taihuN3 in classification, wherein the bacterial strain is preserved in General Microbiology Centre of China Committee for Culture Collection of Microorganisms; the preservation number is 23 Nov 2012; and the preservation number is CGMCC No.6886. Application of the sulfate reducing bacteria with tolerance on arsenic in wastewater treatment is also disclosed by the invention. The bacterial strain disclosed by the invention is wide in utilizable carbon source range, easy to cultivate, strong in growth metabolic activity, and high in sulphate conversion efficiency. The bacterial strain disclosed by the invention can normally grow when the concentration is 10-1000mg/LAs(V); and the percent reduction of the bacterial strain disclosed by the invention under 200mg/LAs(V) of sulfate achieves 50%. The sulfate reducing bacteria can be used for processing high-concentration arsenic wastewater, and has good application prospect.