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466 results about "Sulfate-reducing bacteria" patented technology

Sulfate-reducing microorganisms (SRM) or sulfate-reducing prokaryotes (SRP) are a group composed of sulfate-reducing bacteria (SRB) and sulfate-reducing archaea (SRA), both of which can perform anaerobic respiration utilizing sulfate (SO₄²⁻) as terminal electron acceptor, reducing it to hydrogen sulfide (H₂S). Therefore, these sulfidogenic microorganisms "breathe" sulfate rather than molecular oxygen (O₂), which is the terminal electron acceptor reduced to water (H₂O) in aerobic respiration.

Apparatus for on-site production of nitrate ions

An apparatus and method produces nitrate ions on-site from water, natural gas and air extracted in proximity to the apparatus. The apparatus generates nitrate ions and brings the nitrate ions into contact with an aqueous system. Hydrogen sulfide present in the aqueous system is removed and the production of hydrogen sulfide by sulfate-reducing bacteria (SRB) is eliminated by introducing into the system nitrate ions, whereby denitrifying microorganisms, using nitrate, outcompete the sulfate-reducing bacteria for the available carbon nutrients, thus preventing the SRB from producing hydrogen sulfide. Nitrate ions generated by the apparatus and added to the aqueous system which contains the denitrifying microorganisms can enhance oil recovery by means of microbial enhanced oil recovery mechanisms.
Owner:NITRA GEN LLC

Method for restoring mine entironment

A method of repairing mine ecological environment comprises the treatment of mine environment contamination, wherein the tailings, the barren rock, the smelting slag, the waste water from ore cleaning, the waste water from smelting, the acid mine drainage and the ore yard leachwater which are generated from mine development are collected into the tailings storage, at the same time, sludge and organic matter capable of being degradated by microbiology are added therein, and then the tailings storage is given an anaerobic environment artificially, so that, under the action of microbiology and sulfate reducing bacteria, sulfur ion is generated and the pH value of the tailings storage is increased, a plurality of heavy metal ions are cured and settled by sulfur ion to avoid migration, a biomembrane layer-sulfate reducing bacteria reducing-induction biomineralization precipitation composite geochemical barrier is generated in the tailings storage. The repairing of mine ecological environment is realized by the treatment of mine environment contamination. The present invention can be used for tailings storage pollution prevention in tour, and can be used for closing the repairing of mine ecological environment, or can be used for designing new tailings storage by using the technology of the present invention.
Owner:ELION ECOLOGICAL RESTORATION

Biological Clean Fuel Processing Systems and Methods

Methods and systems to achieve clean fuel processing systems in which carbon dioxide emissions (1) from fossil fuel consumption sources (2) may be processed in at least one processing reactor (4) containing a plurality of chemoautotrophic bacteria (5) which can convert the carbon dioxide emissions into biomass (6) which may then be used for various products (21) such as biofuels, fertilizer, feedstock, or the like. Sulfate reducing bacteria (13) may be used to supply sulfur containing compounds to the chemoautotrophic bacteria (5).
Owner:WESTERN RES INST INC

Fracturing fluids for delayed flow back operations

It has been discovered that certain fracturing fluid compositions can be used to fracture a subterranean formation and be permitted to stay within the formation for a relatively extended period of time, for example 28 days or more, before being flowed back or produced. At least two embodiments are envisioned, a bacteria-containing formulation and an anti-bacterial formulation. Both systems would be expected to prevent the potential of the fluid to oil wet the formulation (water block condition) by keeping the formation water wet through the use of water wetting surfactants or solvents. Additionally, both formulations would control reservoir crude souring (H2S generation by in situ sulfate-reducing bacteria), reservoir plugging (via slime biopolymers generated by in situ microbes, inorganic scale deposition like calcium carbonate or barium sulfate, and clay fines migration).
Owner:SUPERIOR ENERGY SERVICES LLC

Method for treating heavy metal-containing acidic waste water and recycling heavy metal with biomass fixed bed

The invention discloses a method for treating acidic waste water containing heavy metal and reclaiming the heavy metal through a biomass fixed bed, which is characterized in that dry solid biomass capable of being decomposed by microbes is granulated and filled into a container with a supporting layer and a water distributing device so as to form a fixed bed microbe reactor; sewage disposal plant sludge containing sulfate reducing bacteria is taken as the source of microbe bacteria on the fixed bed, and the microbes are cultured, proliferated and acclimated to adapt to the environment of the biomass filler fixed bed through the backflow circulation of the outputted water or taking ferrous sulfate aqueous solution as the inputted water; acid effluent containing heavy metallic ions is led into the fixed bed for direct filtration; and when the water filtering speed can not meet the preset requirements, the operation is stopped, substances in the fixed bed are taken out, the heavy metal is reclaimed through smelting, and the fixed bed microbe reactor is refilled. The method is suitable for waste water containing Cr2O7 <2->, AsO3 <2->, Cu <2+>, Hg <2+> Ag <+>, Cd <2+> and the like. The method can be used for reclaiming the valuable metal resources with low waste water treatment cost.
Owner:HEFEI UNIV OF TECH

Multifunctional preparation for controlling biological corrosion and blockage of oilfield production system

The invention relates to a multifunctional preparation for controlling biological corrosion and blockage of an oilfield production system. The contents of functional preparations in the treating water are as follows: 20 to 200 ppm of disinfectant: glutaraldehyde, acrolein, dodecyldimethylbenzyl ammonium chloride or dodecyldimethylbenzyl ammonium bromide, 10 to 100 ppm of inhibitor: sodium nitrateor sodium nitrite; and / or 10 to 20 ppm of sodium molybdate, sodium tungstate or lithium molybdate; 10 to 20 ppm of corrosion inhibitor: imidazoline quaternary ammonium; or / and 20 to 50 ppm of desulphurizing reagent: trihydroxymethyl nitromethane; or / and 10 to 20 ppm of penetrating agent: alkylpheol ethoxylates. The components undergo synergistic action so that the concentration of the single component is reduced obviously and reaches 25 to 90%, and the efficiency of killing the SRB (Sulfate Reducing Bacteria) reaches more than 90%.
Owner:PETROCHINA CO LTD

Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil

The invention discloses a bacterial strain b capable of resisting the growth of heavy metal Cd and reducing sulfate, and a bacterial strain capable of resisting the growth of the heavy metal Cd and solubilizing phosphate. The classification and name of the bacterial strain b and the bacterial strain are respectively enterbacter ludwigii SRB-2-5U-2 and pseudochrobactrum saccharolyticum LB-4-4-1c, which are already preserved in the Common Microbe Center of the China Committee for Culture Collection of Microorganisms. The bacterial strains disclosed by the invention can be used for simultaneously remediating the surface soil, deep soil and rhizosphere soil of a plant, reducing the pollution of Cd to surface water and underground water, and also improving the activity and fertility of the soil.
Owner:西安金博瑞生态科技有限公司

Preparation method of methane dry fermentation compound bacterial preparation

The invention discloses a preparation method of a methane dry fermentation compound bacterial preparation, which comprises the following steps: mixing fermentation substrate with an inoculum which is compound bacteria composed of cellulose decomposing bacteria, proteolytic bacteria, fat decomposing bacteria, hydrogen-producing aceogenic bacteria, sulfate reducing bacteria and methanogenic archaea, adjusting the water content and pH of the mixed materials to 70-80% and 7.0 respectively, performing anaerobic fermentation under the condition of 35 DEG C plus or minus 2 DEG C for 60-90 days to obtain methane dry fermentation compound bacterial preparation. The preparation method of the invention can prepare compound bacterial preparation with a relatively complete methane fermentation function, and the total number of microbial cells and methanogenic archaea cells in the bacterial preparation are at least 10*10<10> / g and 1.0*10<8> / g respectively. Using the compound bacterial preparation as an inoculum of methane dry fermentation can reduce the starting time, and the fermentation process is stable and easy to control.
Owner:BIOGAS SCI RES INST MIN OF AGRI

Inhibition of biogenic sulfide production via biocide and metabolic inhibitor combination

Biogenic sulfide production is synergistically inhibited by treating sulfate-reducing bacteria (SRB) with a biocide and a metabolic inhibitor. The biocide directly kills a first portion of the SRB. The metabolic inhibitor inhibits sulfate-reducing growth of a second portion of the SRB without directly killing the second portion of the SRB. The treatment of SRB with both a biocide and a metabolic inhibitor provides effective biogenic sulfide inhibition at significantly lower concentrations than would be required if the biocide or metabolic inhibitor was used alone.
Owner:UNIV TECH INT +1

Biological PRB (permeable reactive barrier) system for underground in-situ remediation of coal mine acid wastewater

The invention relates to a biological PRB (permeable reactive barrier) system for underground in-situ remediation of coal mine acid wastewater, and belongs to the technical field of water pollution control. The system comprises a water inlet groove, a biological PRB body and a water outlet tank, wherein the water inlet groove is higher than the goaf ground for 0.3-0.5 m; the biological PRB body adopts a four-layer structure, and sequentially consists of a buffer protecting barrier, a ferrous powder PRB, a biological medical stone PRB and a buffer protecting barrier; the water outlet tank adopts the sectional separating plate type, so as to ensure that the interior of the biological PRB is always in the water filling anaerobic state in the running process of system. The system is arranged on the coal mine goaf, and the defects of land occupation during ground treatment, pollution to environments, laying of long-distance conveying pipelines and the like are overcome. The system has the advantages that the structure is simple, the cost is low, the safety is realized, the efficiency is high, and the manned watching is not required in the running process. On the basis of the traditional PRB, the novel PRB with biological strengthening function is established in the method that the medical stone is fixed and mixed with sulfate reducing bacteria.
Owner:LIAONING TECHNICAL UNIVERSITY

Method for increasing waste water treating efficiency by improving activity of sulfate reducing bacteria

The present invention belongs to the waste water treating technology utilizing sulfate reducing bacteria. Upwards flowing anaerobic composite bed bioreactor and mixed sulfate reducing bacteria are used in treating acid waste water containing Cr2O72-, CrO42-, Cu2+, Zn 2+, Mn2+ and other heavy metal ions. The present invention has high activity of sulfate reducing bacteria and high waste water treating efficiency. The present invention features that inside the reactor or waste water tank, simple substance iron is added to result in waste water pH value of 5.5-8.5, and that the biochemical reaction is performed at 25-30 deg.c. The present invention has the advantages of addition of simple substance iron, raised waste water pH value, improved bacteria living environment, high metabolic activity of the bacteria, reinforced capacity of the sulfate reducing bacteria, raised bacteria adaptability and waste water treating capacity.
Owner:TIANJIN UNIV

Device and method for comprehensively treating acidic sulfate organic waste water based on biological desulfurization

The invention belongs to the field of environmental engineering, and in particular relates to a device and a method for comprehensively treating acidic sulfate organic waste water based on biological desulfurization. The method comprises the following steps of: 1, reducing sulfate in the organic waste water, blowing hydrogen sulfide off, namely (1-1) mixing the acidic sulfate organic waste water and sulfide-containing recirculation water in an anaerobic digestion reactor, controlling the pH of the mixture to be 5.0 to 6.0, and thus obtaining a hydrogen sulfide-containing acidic mixed solution, and (1-2) introducing inert gas, blowing the hydrogen sulfide off, adding sulfate reducing bacteria, reducing the sulfate to form sulfide, obtaining the sulfide-containing recirculation water and returning to the step (1-1); and 2, absorbing, oxidizing and recycling the hydrogen sulfide, namely (2-1) absorbing the hydrogen sulfide blown off in the step (1-2) by using a weak alkaline solution, adding sulfur-oxidizing bacteria, oxidizing the hydrogen sulfide to form elemental sulfur, separating to obtain the elemental sulfur and effluent, and (2-2) treating the effluent for recycling. By the device and the method, sulfate innocent treatment and resource recovery treatment are realized.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Microorganism for controlling harm of oil field sulfate reductive bacterium and application thereof

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g / L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

Chemical control method for corrosion microorganism in pipeline conveyance system and product

The invention relates to three compound stabilizer reagents which can effectively protect various water pipelines, water containers and the relevant equipment of the water conveyance system from biodeterioration or scaling and the treatment method and the utilization of the biodeterioration. Particularly, by utilizing the three compound stabilizer reagents acting on the water conveyance system in different period respectively, the addition dosage and the time can change according to different water quality, different microorganism pollution degree and different water treatment demand, wherein each compound stabilizer reagent main component has different sterilization mechanisms and the emphases of the reagent is also different, so that serious corrosion caused by the drug resistance of the bacteria can be solved and the sulfate reducing bacteria, the saprophyte, the iron bacteria, the nitrite bacteria, the ammonium bacteria and algae in the water conveyance system can be completely killed. The compound stabilizer reagents is suitable for the control of pipeline scaling and the pipe wall biodeterioration of the oil field water injection system, the circulating water system and the central air-conditioning cooling water system.
Owner:WUHAN TEXTILE UNIV

Method for treatment of metal waste water by sulfate reducing bacteria growing up with electric current

The invention belongs to the technical field of treatment of waste water, and particularly relates to a method for reduction of sulfate into sulfide and treatment of metal waste water by sulfate reducing bacteria growing up with electric current. The sulfate reducing bacteria provided by the invention is a mixing bacteria group, which comprises Desulfobulbus propinonicus, geobacter sulfurreducens. Under the condition of lacking organic carbon resource, an electrode is used as the only electronic donator for growing, sulfate is reduced into sulfide via bioelectrical chemistry, and the sulfide is applied to the treatment of metal waste water. According to the method provided by the invention, the defect that organic carbon resource is needed to be used as the electronic donator is overcome,the operation is simple, the secondary pollution is zero, the cost is low, and is particularly suitable for the treatment on organic carbon metal waste water of mine waste water, metal smelting wastewater, electric plating waste water and the like.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S

Prepn and application in industrial effluent treatment of biomembrane carrier

The present invention relates to one kind of functional biological carbon fiber and its preparation process and application as biomembrane carrier. The biomembrane carrier is prepared with polyacrylonitrile carbon fiber, and through electrochemical surface treatment in 5-20 % concentration ammonium bicarbonate electrolyte to increase the surface acid oxygen containing functional groups and raise wettability, and gluing with water soluble epoxy resin in the amount 0.5-2 %. The biomembrane carrier can fix nitrobacteria, sulfate reducing bacteria and anaerobic methanogen. It may be used in treating high concentration acrylon waste water, and can eliminate sulfate, COD and ammonia nitrogen effectively. In addition, the biomembrane carrier is easy to regenerate and has no secondary pollution.
Owner:BEIJING UNIV OF CHEM TECH

Anti-bacterial anti-corrosive coating material for inner wall of oilfield reinjection water pipeline

The invention relates to an anti-bacterial anti-corrosive coating material for the inner wall of an oilfield reinjection water pipeline, comprising solvent-free organic fluorine modified epoxy resin as base material and hybridized organic-inorganic fungicide and filling materials, etc. The coating material has good anti-bacterial performance and anti-corrosive performance, and has good self-cleaning and antisetoff performance. The coating material has good workability, and can be used on wet metal surface. The coating material has fast drying speed, good thoughness and rigidity, good adhesiveforce with the base coat,and flat and smooth film. The coating material has good bactericidal effect on sulfate reducing bacteria, iron bacteria and the like, good broad-spectrum bactericidal action,fast sterilization speed, high sterilization efficiency, no pollution to the environment, and no toxicity to human body.Thereby, the coating material has a wide application prospect.
Owner:SINOPEC PETROLEUM ENG DESIGN

Treatment method of soil contaminated by uranium or cesium

The invention belongs to the technical field of the microorganism, and in particular to a treatment method of soil contaminated by radioactive nuclide uranium or cesium. The invention adopts a bar screen, a double-plate screen and a spiral screen to screen the soil contaminated by uranium or cesium. The soil particles with the particle size more than 0.25mm are clean soil particles, the soil particles with the particle size less than 0.25mm are soil contaminated by uranium or cesium; and the soil particles with the particle size less than 0.25mm are mixed with active acidithiobacillus ferrooxidans for treatment, and the settlement is clean soil after filtration, the filtrate is transferred and mixed with active sulfate reducing bacteria for treatment, then the uranium or cesium is precipitated and can be recovered through filtering separation, and the water can also be recovered. The invention has the advantages of simple operation and low cost, and realizes the rapid and efficient removal of the uranium or cesium in the slightly contaminated soil.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S

Nutrient for inhibiting sulfate reducing bacteria in oilfield water and application of nutrient

The invention relates to a nutrient for inhibiting sulfate reducing bacteria in oilfield water and application of the nutrient. The nutrient contains the following components by weight percent: 20-95 percent of cane molasses or beet molasses, and / or 1-45 percent of ammonium phosphate, and / or 0.5-10 percent of ammonium chloride, and / or 0.5-2.5 percent of ammonium sulfate, and / or 1-18.5 percent of ammonium nitrate, and / or 0.05-49 percent of sodium nitrate; and / or 0.05-1 percent of diammonium hydrogen phosphate, and / or 0.05-1.2 percent of disodium hydrogen phosphate, and / or 0.5-2 percent of dipotassium phosphate and / or 0.05-1.5 percent of potassium dihydrogen phosphate. The nutrient with weight accounting for 0.5-5 percent of weight of filled water is taken, is dissolved by using constructional injection water or oilfield surface water and is squeezed into stratum by a pump truck through a water injection well. The nutrient can activate nitrifying bacteria flora in source bacteria flora of stratum water or oilfield source water and can inhibit the growth of sulfate reducing bacteria. After 16 hours to 52 days, the content of sulfate reducing bacteria is 0-102 / mL. The yield of crude oil is increased by 10 percent to 30 percent.
Owner:PETROCHINA CO LTD

Method for biological treatment of sulfate wastewater employing synchronous electric catalysis of anode and cathode

ActiveCN103319002AImprove the reduction of SO <sub>4</sub> <sup>2-</sup> rateReduce consumptionTreatment with aerobic and anaerobic processesSulfate-reducing bacteriaSulfur
The invention provides a method for biological treatment of sulfate wastewater employing synchronous electric catalysis of an anode and a cathode. The method comprises the following concrete steps of: (1) building an electric catalytic bioreactor, which includes a cathode reaction zone, a buffer zone and an anode reaction zone; (2) inoculating mixed bacteria of sulfate reducing bacteria inside the cathode reaction zone, leading to a culture medium, electrifying and acclimatizing, and cultivating for 3-5 days at 28-34 DEG C, so as to achieve cathode hanging membrane of the sulfate reducing bacteria; (3) inoculating sulfur-oxidizing bacteria inside the anode reaction zone, leading to the culture medium to cultivate for 5-7 days at 25-30 DEG C; and (4) starting a reactor, leading the sulfate wastewater to the cathode reaction zone of the reactor, and enabling the sulfate wastewater to flow out of the reactor after flowing inside the anode reaction zone through the buffer zone. By adopting the method, control of an S<2->oxidization process is achieved when the reduction speed of the sulfate reducing bacteria for reducing SO4<-2> is improved. Thus, the yield of S produced by biological metabolism of the wastewater is improved.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

System and methods for biological selenium removal from water

A biological system for removing selenium from waste water comprises a first immobilized cell bioreactor (ICB) and a selenide removal module. The first ICB comprises a chamber having a substrate housed therein and situated to contact the waste water flowing therethrough during use. Anaerobic microorganisms are supported on the substrate, and comprise selenium respiring bacteria capable of reducing selenates and selenites to insoluble elemental selenium and / or sulfate reducing bacteria capable of reducing selenates and selenites to insoluble elemental selenium or to soluble selenides. The selenide removal module includes metallic or oxidized iron compounds capable of chemically reacting with selenide or sulfide compounds in the waste water to form iron selenide or iron sulfide precipitates.
Owner:UOP LLC

Method and apparatus for removing sulfate in high-salt petrochemical wastewater

The invention relates to a removing method and a removing apparatus for sulfate in high-salt petrochemical wastewater, and belongs to the technical field of wastewater treatment. According to the present invention, in order to provide a method with characteristics of low cost, simpleness, convenience and high sulfate removal rate, and an apparatus thereof, according to the principle of conversion of sulfate into hydrogen sulfide with sulfate reducing bacteria, residual sludge produced by a high-salt petrochemical wastewater treatment system precipitation tank is subjected to ultrasonic wave degradation through an ultrasonic wave treatment, the obtained sludge or sludge supernatant and high-salt petrochemical wastewater are mixed, and then enter an anoxic tank or anaerobic tank so as to provide a carbon source for the sulfate reducing bacteria, and a blowing removing manner under a N2 solution is adopted to remove the hydrogen sulfide so as to reduce inhibition effects of hydrogen sulfide and other sulfides on the sulfate reducing bacteria; and the sulfate reduction can be effectively promoted, the sulfate removal rate can be increased, the calcium sulfate scaling tendency can be reduced, the maintenance period and the service life of the equipment can be prolonged, and the system stability is increased.
Owner:CHINA PETROLEUM & CHEM CORP

Oil field sewage bactericide and preparation method thereof

The invention discloses an oil field sewage bactericide and a preparation method thereof, belonging to the technical field of chemical agents for oil fields and preparation thereof. The oil field sewage bactericide is prepared by mixing the following components in percentage by mass: 10-15% of nano titanium dioxide powder, 0.5-2% of crosslinking agent, 5-10% of surfactant, 5-10% of oxidizer and the balance of water. The bactericide can be deposited on pipe walls, bent angles and other parts along with a liquid flow; a main agent can be slowly diluted out, has persistent chemical action and causes no corrosion to pipelines; the comprehensive sterilizing rate of the bactericide with respect to sulfate reducing bacteria, saprophytic bacteria and iron bacteria can be still up to 90% or above 18 days after filling; and the filling period of the chemical agent is 15-25 days which is 10-15 times longer in comparison with a common chemical agent, thereby greatly relieving the operation intensity and lowering the cost.
Owner:CHINA PETROLEUM & CHEM CORP +1

Method and device for treating oily sludge

InactiveCN102001806AImprove qualityQuickly break down complex structuresSludge treatment by oxidationElectrochemical responseMicro bubble
The invention relates to a method and a device for treating oily sludge. Under the action of an applied electric field and by adopting an electrochemical oxidation method, the oil-in-water and water-in-oil structure of emulsified oil is quickly broken, and the oily sludge serving as an electrolyte releases oil drops; oil is quickly taken by a large number of micro bubbles to the liquid level to be recycled under the action of applied air flotation; oily sludge flocs are subjected to electrochemical reaction on the surface to produce various active free radicals which have high activity and oxidability and mainly comprise hydroxyl, and organic pollutants are degraded to be converted into water and carbon dioxide; the active free radicals can destroy a microbial cell structure and kill sulfate reducing bacteria and saprophytic bacteria through a series of oxidation chain reactions; the sludge is powerfully dehydrated, so that the water quality of dehydrated water meets the water quality standard of oil field reinjection, and the sludge can be recycled; and a mud cake is nontoxic and harmless and can be used for soil improvement, fertilizers, building materials and the like.
Owner:朱同德 +1

Sterilizing stripper dedicated for oilfield injection water and preparation method thereof

The invention belongs to the technical field of water treatment, and particularly relates to a sterilizing stripper dedicated for oilfield injection water and a preparation method thereof. The sterilizing stripper dedicated for the oilfield injection water is characterized by compounding the following materials in percentage by weight: 15 to 35 percent of tetrakis hydroxymetyl phosphonium sulfuric, 10 to 35 percent of dodecyl dimethyl benzyl ammonium chloride, 0.5 to 1.0 percent of sodium dioctylsulfosuccinate, and the balance of deionized water. The preparation method comprises the followingsteps: adding the deionized water into a reaction kettle; and adding the tetrakis hydroxymetyl phosphonium sulfuric, the dodecyl dimethyl benzyl ammonium chloride and the sodium dioctylsulfosuccinate, and stirring for 40 to 50 minutes at normal temperature. By the method, the stripping performance of the sterilizing stripper and the killing performance to bacteria, particularly sulfate reducing bacteria, are improved; the sterilizing stripper has strong sterilization capability, good penetration and remarkable stripping effect; and the preparation process is simple and easy to operate.
Owner:山东合润环境科技有限公司

Method of using native function microflora to in-situ purify ground water polluted by uranium

The invention relates to a method of using native function microflora to in-situ purify ground water polluted by uranium. The method translates transferable hexavalent uranium into nontransferable uranous uranium in the ground water through nitrate reducing bacteria flora, ferric iron reducing bacteria flora and sulfate reducing bacteria flora which are formed under the anaerobic condition by microorganism, and therefore the purpose of purifying the ground water polluted by the uranium is achieved. The specific implementation method comprises: (1) adjusting a potential of hydrogen (Ph) value of the ground water polluted by the uranium; (2) activating the microorganism in sediments; (3) regulating and controlling variation of the microflora, accelerating evolution of the nitrate reducing bacteria flora, the ferric iron reducing bacteria flora and the sulfate reducing bacteria flora by means of adding a carbon source, regulating oxidation reduction potential of the water polluted by the uranium and the like; (4) continuously regulating and controlling the microbial flora until uranium concentration in the ground water polluted by the uranium is reduced below the national emission standard and the purpose of purifying the groundwater polluted by the uranium is achieved. The method of using the native function microflora to in-situ purify the ground water polluted by the uranium solves in-situ purifying problem of the ground water polluted by the uranium, and has the advantages of being high in purifying efficient, short in period, simple in equipment, short in process procedure, low in purifying cost, strong in operability, free of secondary pollution, good in purifying effect and the like.
Owner:NANHUA UNIV

Method for biologically inhibiting production of secondary hydrogen sulfide from sulfate reducing bacteria in crude oil gathering and transporting system

The invention relates to a method for biologically inhibiting the production of secondary hydrogen sulfide from sulfate reducing bacteria in a crude oil gathering and transporting system. In the method, (1) a biological inhibitor for inhibiting a single well consists of 50 mg / L sodium nitrite and 20 mg / L sodium borate; (2) the biological inhibitor for inhibiting an oil transfer station consists of 40 to 60 mg / L sodium nitrite, 25 milligrams of humic acid, 10 to 20 mg / L sodium borate and 20 to 30 mg / L sodium hydroxide electrolyte; and (3) the biological inhibitor for inhibiting a combined station consists of 40 to 60 mg / L sodium nitrite, 25 milligrams of humic acid, 10 to 20 mg / L sodium borate, 20 to 30 mg / L sodium hydroxide electrolyte and 95 mg / L glutaraldehyde bactericide. In the method, the generation of the hydrogen sulfide is controlled at the source, and the method has the characteristics of long hydrogen sulfide inhibition time, low cost and no secondary pollutions and improvesthe working environment of a workplace of the crude oil gathering and transporting system.
Owner:PETROCHINA CO LTD

Complex oilfield water flooding bactericide

The present invention discloses a complex oilfield water flooding bactericide. The complex oilfield water flooding bactericide is prepared from the following raw materials by weight: 25-35 parts of saccharide gemini quaternary ammonium salt, 2-5 parts of alkyl glucoside, 10-15 parts of quaternary phosphonium salt, 4-10 parts of isothiazolinone, and 5-10 parts of dithiocyano-methane. The bactericide has strong bactericidal effect, can effectively remove the sulfate-reducing bacteria, iron bacteria and saprophytes in water, and avoid corrosion and clogging of pipelines and equipment caused by bacteria, and has the advantages of simple ingredients, easy preparation, fastness, efficiency, low cost, high sterilization rate, low usage and little side effects.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY

Sulfate reducing bacteria with tolerance on arsenic

InactiveCN103013868AWide range of carbon sourcesEasy to trainBacteriaWater contaminantsHigh concentrationMicroorganism
The invention discloses sulfate reducing bacteria with tolerance on arsenic. The sulfate reducing bacteria are named as enterobacteria (enterobacter sp.) taihuN3 in classification, wherein the bacterial strain is preserved in General Microbiology Centre of China Committee for Culture Collection of Microorganisms; the preservation number is 23 Nov 2012; and the preservation number is CGMCC No.6886. Application of the sulfate reducing bacteria with tolerance on arsenic in wastewater treatment is also disclosed by the invention. The bacterial strain disclosed by the invention is wide in utilizable carbon source range, easy to cultivate, strong in growth metabolic activity, and high in sulphate conversion efficiency. The bacterial strain disclosed by the invention can normally grow when the concentration is 10-1000mg / LAs(V); and the percent reduction of the bacterial strain disclosed by the invention under 200mg / LAs(V) of sulfate achieves 50%. The sulfate reducing bacteria can be used for processing high-concentration arsenic wastewater, and has good application prospect.
Owner:NANJING UNIV
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