Microorganism for controlling harm of oil field sulfate reductive bacterium and application thereof

Abstract

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g / L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.

Description

technical field

[0001] The invention belongs to the field of microbial preparations, in particular to a microbial preparation for controlling the harm of sulfate-reducing bacteria in oilfields and an application thereof, and is a microbial control technology suitable for controlling the quantity and activity of sulfate-reducing bacteria (SRB) in oilfield secondary oil recovery. Background technique

[0002] In the petroleum industry, the corrosion of surface and underground facilities and sulfide pollution caused by microorganisms are very serious, which have caused serious economic, environmental and safety production problems. The massive reproduction of sulfate-reducing bacteria in the water injection system is the main cause of this hazard. At present, the sulfate-reducing bacteria (SRB) contained in the produced water system of the oilfield secondary recovery process can produce a large amount of H 2 S and sulfides, leading to corrosion and sulfide pollution. The main...

Examples

Embodiment 1

[0028] Firstly, the denitrifying bacteria culture is prepared by conventional aerobic fermentation technology according to the ratio, and then the nutrient elements, synergistic nutrient elements and the denitrifying bacteria culture are uniformly mixed. Alternatively, when in use, denitrifying bacteria culture, nutrient elements, and synergistic nutrient elements may be added separately.

[0029] The composition of the microbial preparation and its content in the treated water are as follows:

[0030] 1) Denitrifying bacteria culture composition: Pseudomonas putida 5×10 7 Bacteria / mL, water balance; denitrifying bacteria culture addition is 1%wt of the treated water;

[0031] 2) Sodium nitrate: 5mg / L;

[0032] 3) Sodium molybdate or lithium molybdate: 20ppm.

Embodiment 2

[0034] The difference from Example 1 is that the composition of the microbial preparation and the content in the treated water are as follows:

[0035] 1) Denitrifying bacteria culture composition: Pseudomonas putida 2×10 8 bacteria / mL, water balance; denitrifying bacteria culture addition is 0.5%wt;

[0036] 2) Sodium nitrite: 10mg / L;

[0037] 3) Sodium tungstate: 10ppm.

Embodiment 3

[0039] The difference from Example 1 is that the composition of the microbial preparation and the content in the treated water are as follows:

[0040] 1) Composition of denitrifying bacteria culture: Thiobacillus denitrificans 2×10 8 bacteria / mL, water balance; denitrifying bacteria culture addition is 0.01%wt;

[0041] 2) Sodium nitrite and sodium phosphate: 50mg / L each;

[0042] 3) Sodium tungstate: 100ppm.

[0043] Microbial preparations are continuously or intermittently added to the surface water treatment system or / and reinjection water system, the dosage rate of continuous dosage is 10-500L / min; the dosage speed of intermittent dosage is 10-1000L / min, Dosing for 1-12 hours per day, resting for 23-12 hours (the process parameters of microbial preparations should be determined according to the relevant parameters such as the water injection speed and quantity on the oil well site)

[0044] Method 1: Dynamic dosing of surface system

[0045] Taking Example 1 as an exa...

technical field

[0001] The invention belongs to the field of microbial preparations, in particular to a microbial preparation for controlling the harm of sulfate-reducing bacteria in oilfields and an application thereof, and is a microbial control technology suitable for controlling the quantity and activity of sulfate-reducing bacteria (SRB) in oilfield secondary oil recovery. Background technique

[0002] In the petroleum industry, the corrosion of surface and underground facilities and sulfide pollution caused by microorganisms are very serious, which have caused serious economic, environmental and safety production problems. The massive reproduction of sulfate-reducing bacteria in the water injection system is the main cause of this hazard. At present, the sulfate-reducing bacteria (SRB) contained in the produced water system of the oilfield secondary recovery process can produce a large amount of H 2 S and sulfides, leading to corrosion and sulfide pollution. The main...

Examples

Embodiment 1

[0028] Firstly, the denitrifying bacteria culture is prepared by conventional aerobic fermentation technology according to the ratio, and then the nutrient elements, synergistic nutrient elements and the denitrifying bacteria culture are uniformly mixed. Alternatively, when in use, denitrifying bacteria culture, nutrient elements, and synergistic nutrient elements may be added separately.

[0029] The composition of the microbial preparation and its content in the treated water are as follows:

[0030] 1) Denitrifying bacteria culture composition: Pseudomonas putida 5×10 7 Bacteria / mL, water balance; denitrifying bacteria culture addition is 1%wt of the treated water;

[0031] 2) Sodium nitrate: 5mg / L;

[0032] 3) Sodium molybdate or lithium molybdate: 20ppm.

Embodiment 2

[0034] The difference from Example 1 is that the composition of the microbial preparation and the content in the treated water are as follows:

[0035] 1) Denitrifying bacteria culture composition: Pseudomonas putida 2×10 8 bacteria / mL, water balance; denitrifying bacteria culture addition is 0.5%wt;

[0036] 2) Sodium nitrite: 10mg / L;

[0037] 3) Sodium tungstate: 10ppm.

Embodiment 3

[0039] The difference from Example 1 is that the composition of the microbial preparation and the content in the treated water are as follows:

[0040] 1) Composition of denitrifying bacteria culture: Thiobacillus denitrificans 2×10 8 bacteria / mL, water balance; denitrifying bacteria culture addition is 0.01%wt;

[0041] 2) Sodium nitrite and sodium phosphate: 50mg / L each;

[0042] 3) Sodium tungstate: 100ppm.

[0043] Microbial preparations are continuously or intermittently added to the surface water treatment system or / and reinjection water system, the dosage rate of continuous dosage is 10-500L / min; the dosage speed of intermittent dosage is 10-1000L / min, Dosing for 1-12 hours per day, resting for 23-12 hours (the process parameters of microbial preparations should be determined according to the relevant parameters such as the water injection speed and quantity on the oil well site)

[0044] Method 1: Dynamic dosing of surface system

[0045] Taking Example 1 as an exa...

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