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Preparation method of methane dry fermentation compound bacterial preparation

A compound bacterial agent, dry fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problems of narrow use range, no mention of dry fermentation, etc., and achieve low cost, rich enzyme production, Removing the effects of feedback inhibition

Inactive Publication Date: 2012-07-11
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its microbial composition is mainly designed for the degradation of cellulose, and it is used for conventional biogas fermentation with rice straw as the main raw material. The use range of this microbial agent is narrow, and it is not mentioned for dry fermentation

Method used

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  • Preparation method of methane dry fermentation compound bacterial preparation
  • Preparation method of methane dry fermentation compound bacterial preparation
  • Preparation method of methane dry fermentation compound bacterial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The composite strain of the present invention is composed of 11 strains of bacteria, and according to the different functions in the fermentation process, these 11 strains of bacteria can be divided into the following 6 categories:

[0044] Cellulolytic bacteria: Bacteroides cellulosilyticus , Clostridium sufflavum ;

[0045] Proteolytic bacteria: Clostridium pasteurianum ;

[0046] Lipolytic bacteria: Syntrophomonas sapovoran s;

[0047] Sulfate reducing bacteria: Desulfovibrio desulfuricans , Desulfobulbus rhabdoformis ;

[0048] Hydrogen-producing acetogenic bacteria: Syntrophomonas Wolfei ;

[0049] Methanogenic archaea: Methanospirillum hungatei , Methanocorpusculum parvum , Methanosarcina barkeri , Methanosaeta concilii .

[0050] The above-mentioned 11 strains of cellulolytic bacteria, proteolytic bacteria, lipolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria and methanogenic archaea were directly mixed ...

Embodiment 2

[0054] The composition ratio of the 11 strains in the inoculum is as follows: Bacteroides cellulosilyticus 22%, Clostridium sufflavum 17%, Clostridium pasteurianum 10%, Desulfobulbus rhabdoformis 11%, Syntrophomonas sapovorans 11%, Syntrophomonas Wolfei 10%, Desulfovibrio desulfuricans 13%, Methanospirillum hungatei accounting for 0.5%, Methanocorpusculum parvum 1.0%, Methanosarcina barkeri 1.5%, Methanosaeta concilii 3%.

[0055] A mixture of pig manure and corn stover was used as fermentation substrate. The corn stalks were pulverized into powder, mixed with pig manure in a ratio of 1:1 by mass, and water was added to adjust the TS content to 30%, and composted at natural temperature for 7 days. After composting, adjust the pH value to 7.0 with lime powder, and then add the above inoculum according to raw material: inoculum = 10:3 (mass ratio), after mixing evenly, the moisture content of the mixture can be adjusted to 80%, and at 35 Anaerobic culture ...

Embodiment 3

[0058] The inoculum consisted of the following 11 strains, Bacteroides cellulosilyticus 20%, Clostridium sufflavum 15%, Clostridium pasteurianum 12%, Desulfobulbus rhabdoformis 11%, Syntrophomonas sapovorans 13%, Syntrophomonas Wolfei 10%, Desulfovibrio desulfuricans 13%, Methanospirillum hungatei 1.0%, Methanocorpusculum parvum 1.5%, Methanosarcina barkeri 2%, Methanosaeta concilii 1.5%.

[0059] A mixture of pig manure and rice straw was used as fermentation substrate. The straw stalks were pulverized into powder, mixed with pig manure in a ratio of 1:1 by mass, added water to adjust the TS content to 25%, and composted for 7 days at natural temperature. After the composting, the pH value was adjusted to 6.5 with lime powder, and the above-mentioned inoculum was added according to the fermentation substrate: inoculum=10:3 (mass ratio). After mixing evenly, anaerobic culture was carried out at 35±2°C. After 60 to 90 days, the compound bacterial agent i...

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Abstract

The invention discloses a preparation method of a methane dry fermentation compound bacterial preparation, which comprises the following steps: mixing fermentation substrate with an inoculum which is compound bacteria composed of cellulose decomposing bacteria, proteolytic bacteria, fat decomposing bacteria, hydrogen-producing aceogenic bacteria, sulfate reducing bacteria and methanogenic archaea, adjusting the water content and pH of the mixed materials to 70-80% and 7.0 respectively, performing anaerobic fermentation under the condition of 35 DEG C plus or minus 2 DEG C for 60-90 days to obtain methane dry fermentation compound bacterial preparation. The preparation method of the invention can prepare compound bacterial preparation with a relatively complete methane fermentation function, and the total number of microbial cells and methanogenic archaea cells in the bacterial preparation are at least 10*10<10> / g and 1.0*10<8> / g respectively. Using the compound bacterial preparation as an inoculum of methane dry fermentation can reduce the starting time, and the fermentation process is stable and easy to control.

Description

technical field [0001] The invention relates to a preparation method of a biogas dry fermentation composite bacterial agent, belonging to the technical fields of organic waste utilization and renewable energy production. Background technique [0002] In recent years, society's demand for energy has been increasing, fossil energy resources such as petroleum and coal have been decreasing, and prices have continued to rise, which has brought adverse effects on people's production and life. workers' attention. Biogas is clean and renewable energy, which is inexhaustible. Organic matter such as straw, poultry manure, and urban organic waste can be converted into biogas through anaerobic digestion, providing clean energy for humans. [0003] Microorganisms involved in biogas fermentation include fermentative bacteria, hydrogen-producing acetogenic bacteria, and methanogenic archaea. First, fermentative bacteria degrade cellulose substances into organic acids such as acetic acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01C12R1/145
Inventor 尹小波李强徐彦胜马诗淳张云飞代丽蓉承磊
Owner BIOGAS SCI RES INST MIN OF AGRI
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