39 results about "Methanosaeta" patented technology

The invention disclosure is a method of bioremediation of wastewater, particularly groundwater, by utilizing coupled anaerobic and aerobic biological treatment, more specifically, methanogenic (strictly anaerobic) and methanotrophic (strictly aerobic) microbial populations, in combination with a supply of in-situ generated water-dissolved oxygen and hydrogen. Water electrolysis is used to produce water-dissolved oxygen and hydrogen. The immediate advantage of using H2 from the electrolysis is to provide electron donors to methanogens to reductively dechlorinate the chloroaliphatics, and to reduce the water carbonates and generate methane which is used as energy and carbon source for the methanotrophic bacteria. Oxygen is used as electron acceptor by the aerobic bacteria, including the methanotrophs. The addition of an organic carbon source can be minimized or even eliminated, so as to diminish the competition between methanotrophic bacteria and heterotrophic bacteria for oxygen.

The present invention provides a method of directional acetic acid production in anaerobic sludge fermentation using methanogenic bacteria-specific inhibitors which belongs to the sludge recycling field. This invention prepares the methanogenic bacteria-specific inhibitor, 2-bromethylsulfonate sodium into solution of certain concentration and adds the solution into the pretreated sludge. When the concentration of 2-bromethylsulfonate sodium is 5-20 mmol / L and the initial pH is 6-8, the maxmium concentration of acetic acid can achieve 1.84g / L after 15-20 day's fermentation, which is more than five times of the yield of acetic acid under natural conditions. The addition of 2-bromethylsulfonate sodium into anaerobic digestion system will inhibit methanogens and facilitates the accumulation of organic acids, especially acetic acid.

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

The invention relates to a method for improving anaerobic digestion performance of sludge and synchronously enlarging cultivation by functional bacteria. The method comprises the following steps of: collecting and screening the functional bacteria; enlarging cultivation of the functional bacteria; preparing functional bacteria solution; inoculating the functional bacteria solution into an anaerobic digestion reactor containing sludge after carrying out anaerobic digestion for 10-15 days on the sludge, further carrying out anaerobic digestion on the sludge for 15-30 days, hydrolyzing protein which is difficult to degrade in the sludge into low molecular weight organic matters by the functional bacteria; hydrolyzing the low molecular weight organic matters to form methane by mathanogenic bacteria; and carrying out multiplication of bacteria cells by the functional bacteria, so as to finally obtain sludge digestion residue containing the methanogenic bacteria and the functional bacteria. Compared with the prior art, advantages enrichment and expansion of the functional bacteria are achieved by the method; the obtained sludge digestion residue contains target functional bacteria, and can be used as an inoculant to be applied to anaerobic digestion treatment of the sludge by circulating inoculation; and the anaerobic digestion efficiency of the sludge is lastingly improved, and the treatment cost is reduced.

The invention belongs to the technical field of environment protection and discloses a compound microbial bacterial preparation and application thereof. The compound microbial bacterial preparation consists of fermentation liquor of four bacteria, i.e. Bacillus licheniformis, Pseudomonas putida, Enterobacter cloacae and Candida valida. The compound microbial bacterial preparation provided by the invention has strong digestion power for various types of cellulose, proteins and fat macromolecules. The addition of the compound microbial bacterial preparation into excess sludge and / or kitchen waste can quicken enrichment of methanogen, shorten anaerobic digestion time and improve methane yield. Moreover, the synergetic effect of the four microbial strains is superior to the effect of any one, two or three strains of microorganisms.

The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement.

This method of restricting methane production in methanogenic bacteria, by the use of the enzyme and coenzyme inhibitors, works during anaerobic reductive dechlorination. Various compounds such as, but not limited to, red yeast rice, vitamin B10 derivatives, and ethanesulfonates are utilized to disrupt these different enzyme and coenzyme systems responsible for the production of methane. This method affects the competition of the methanogen and halo bacteria for the organic hydrogen donors that are injected in the soil and groundwater system during the remediation process.

The invention discloses a ganoderma polysaccharide preparation for regulating rumen microflora and a preparation method thereof. The invention extracts the ganoderma polysaccharide from ganoderma ganoderma residues, ganoderma processing leftovers or ganoderma, and adds a carrier to make a preparation. The preparation is added to the premix or total mixed ration at 5-50 mg / kg dry matter of the ration, and is used to feed cattle and sheep. Filamentous bacteria, Ruminococcus xanthus, Bacillus fibrinolyticus and total bacteria concentration, reduce the concentration of methane bacteria, increase the production of NH3-N, microbial protein and total volatile fatty acids, and at the same time enhance the immunity of cattle and sheep, and improve fiber feed Utilization, improve production performance. Moreover, the source is easy, the preparation process is simple, the use is convenient and safe, and there is no side effect. It can be used as a green additive for cattle and sheep feed, and has broad application prospects.