39 results about "Methanosaeta" patented technology

The invention belongs to the technical field of environment protection and discloses a compound microbial bacterial preparation and application thereof. The compound microbial bacterial preparation consists of fermentation liquor of four bacteria, i.e. Bacillus licheniformis, Pseudomonas putida, Enterobacter cloacae and Candida valida. The compound microbial bacterial preparation provided by the invention has strong digestion power for various types of cellulose, proteins and fat macromolecules. The addition of the compound microbial bacterial preparation into excess sludge and/or kitchen waste can quicken enrichment of methanogen, shorten anaerobic digestion time and improve methane yield. Moreover, the synergetic effect of the four microbial strains is superior to the effect of any one, two or three strains of microorganisms.

The invention discloses anaerobic degradation cellulose methane composite bacteria, which is composed of 13 strains that respectively belong to facultative anaerobic and strictly anaerobic fermentative bacteria, hydrogen-production and acetic acid-production bacteria and methane-producing bacteria. The composite bacteria can not only effectively perform anaerobic degradation to the cellulose, but also can use the degradation production of the cellulose to produce methane, which can be widely used in straw biogas fermentation, compost processing, and the like conversion processes.

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

The invention discloses a composite microbial inoculum used for the anaerobic fermentation of kitchen wastes, belongs to the field of micro-organisms, and solves the problem of the lack of a microbial inoculum suitable for performing anaerobic fermentation by taking kitchen wastes as a main raw material at present. The composite microbial inoculum comprises, in parts by weight, 8-15 parts of acid production bacteroides DSM 15896 fermentation liquor, 25-35 parts of long-chain fatty acid syntrophic aeromonas DSM 18709 fermentation liquor, 15-25 parts of clostridium sordellii ATCC9714 fermentation liquor, 20-28 parts of combined methanosaeta JCM10134 fermentation liquor and 22-27 parts of methanospirllum hungatei DSM864 fermentation liquor, wherein the effective viable counts of the fermentation liquors of the five bacteria aforementioned achieve more than 10<9> per millilitre respectively. The composite microbial inoculum disclosed by the invention is suitable for an anaerobic fermentation process taking the kitchen wastes as the main raw material, and capable of playing the roles of stabilizing the pH value of a fermentation system, promoting to bring forward the peak of methanogenesis, and increasing the total amount of methanogenesis after being inoculated in the fermentation system.

The invention discloses a microbial agent for sewage treatment. The microbial agent comprises, by weight, 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methanosarcina, 3-5 parts of methanosaeta, 6-8 parts of rhodopseudomonas palustris, 4-6 parts of cellulomonas flavigena, 8-10parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of bacillus nitrosomonas, 1-3 parts of issatchenkia orientalis and 3-5 partsof streptomyces bullis. Besides, the invention further discloses an attachment carrier of the microbial agent and a preparation method of the microbial agent. The microbial agent can be effectively attached to the carrier and accordingly has better activity. The microbial agent is reasonable in strain preparation and can be effectively attached to the attachment carrier to treat sewage, and the method for sewage treatment is simple, good in treatment effect and worthy of popularization.

The invention relates to a separation method of monoprop-oxidating hydrogen-producing acetogenic dominant bacterial flora, in particular to a separation method of hydrogen-producing acetogenic bacteria, solving the problem of separation difficulty and low hydrogen-producing rate of hydrogen-producing acetogenic bacteria cultured by using the traditional separation method. The separation method comprises the following steps of: (1) primarily training anaerobic active mud; (2) preparing a bacterial suspension A; (3) enriching the bacterial suspension; (4) secondarily enriching and culturing the enriched bacterial suspension; (5) preparing a compound bacterial flora of monoprop-oxidating hydrogen-producing acetogenic bacteria and methane-producing bacteria; (6) preparing a monoprop-oxidating hydrogen-producing acetogenic bacterial flora; (7) transferring the monoprop-oxidating hydrogen-producing acetogenic bacterial flora into a monoprop culture substrate to be cultured; and (8) repeating the step (7) for 4-8 times for separation. The invention method has easy separation and simple operation, and the hydrogen-producing rate of the separated dominant bacterial flora is about 6-8 times that of the traditional hydrogen-producing bacteria. The dominant bacterial flora of the invention not only can degrade higher-concentration monoprop, but also can effectively improve the processing efficiency of high-concentration organic wastewater.

The present invention provides a method of directional acetic acid production in anaerobic sludge fermentation using methanogenic bacteria-specific inhibitors which belongs to the sludge recycling field. This invention prepares the methanogenic bacteria-specific inhibitor, 2-bromethylsulfonate sodium into solution of certain concentration and adds the solution into the pretreated sludge. When the concentration of 2-bromethylsulfonate sodium is 5-20 mmol/L and the initial pH is 6-8, the maxmium concentration of acetic acid can achieve 1.84g/L after 15-20 day's fermentation, which is more than five times of the yield of acetic acid under natural conditions. The addition of 2-bromethylsulfonate sodium into anaerobic digestion system will inhibit methanogens and facilitates the accumulation of organic acids, especially acetic acid.

The present invention provides a method of hydrogen production from microorganisms, wherein a bioreactor provides an environment conducive to the production of hydrogen from Klebsiella oxytoca and restrictive to the production of methane from methanogens. Klebsiella oxytoca metabolizes organic material at elevated temperatures and pH levels that are detrimental to methanogens. Particularly, the environment in the bioreactor is preferably maintained between about 3.5 and 6.0 pH. Further, the Klebsiella oxytoca metabolizes organic material in the organic feed material and produces hydrogen in substantially a 1:1 ratio with carbon dioxide.

The invention discloses a marsh gas fermentation material and a preparation method thereof, belonging to the technical field of a biological fermentation method for preparing marsh gas. The marsh gas fermentation material comprises a fermentation main material and a fermentation initiator, wherein the fermentation main material is prepared from Flammulina velutipes residue, Enteromorpha, corn straw, fish pond sludge, wheat straw, animal manure and wine stillage; the fermentation initiator is prepared from fish pond sludge, wheat straw, animal manure and strains; the strains comprise Bacteroides succinogenes, Butyrivibrio fibrisolvens, Bacillus subtilis, microzyme, sarcina methanica and Methanosaeta in a ratio of 1:5:3:3:2:2; and the weight ratio of the fermentation main material to the fermentation initiator is (15-19):1. The marsh gas fermentation material fully utilizes the wastes Flammulina velutipes residue and Enteromorpha, and can greatly shorten the start time and increase the gas production quantity and gas production speed in the marsh gas fermentation process.

The invention discloses methanomassiliicoccus sp. The classification name of the methanomassiliicoccus sp. is methanomassiliicoccus sp. (methanomassiliicoccus sp.), the bacterial strain number is LGM-RCC1, the methanomassiliicoccus sp. is preserved in the China General Microbiological Culture Collection Center, the preservation time is at 24 October, 2018, and the preservation number is CGMCC No.16819. The invention also discloses an application of the methanomassiliicoccus sp. in fermentation production of methane, and an application of the methanomassiliicoccus sp. to preparation of a probiotic agent or a feed additive. The LGM-RCC1 can be used for generating the methane through reducing methanol, monomethylamine, dimethylamine, trimethylamine and methyl 3-methylthiopropionate through hydrogen, and the LGM-RCC1 can be used as an additive for increasing the yield of the methane in a methane-generating pit; and the LGM-RCC1 can utilize the trimethylamine in digestive tracts, toxicity of the LGM-RCC1 to human bodies or animals can be reduced, and the LGM-RCC1 is a potential probiotic for treating diseases and preventing cardiovascular diseases caused by trimethyl amine oxide.

A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus, and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.

The invention discloses a separation method of butyric acid oxidized hydrogen-producing acetogenic dominant microorganisms, relating to a separation method of hydrogen-producing acetogenic bacteria and solving the problems that the traditional separation method is difficult to separate and the hydrogen-producing rate of the cultured hydrogen-producing acetogenic bacteria is low. The separation method comprises the following steps of: firstly, preliminarily domesticating anaerobic activated sludge; secondly, preparing a bacterial suspension A; thirdly, enriching a back bacterial suspension; fourthly, enriching and culturing the enriched back bacterial suspension again; fifthly, preparing compound microorganisms of butyric acid oxidized hydrogen-producing acetogenic bacteria and methanogen; sixthly, preparing the butyric acid oxidized hydrogen-producing acetogenic microorganisms; seventhly, transferring the butyric acid oxidized hydrogen-producing acetogenic microorganisms to a butyric acid culture medium for vibration velocity culture; and eighthly, repeating the step seven for 3-6 times and then separating. The method has easy separation and simple operation, and the hydrogen-producing rate of the separated dominant microorganisms is about 7-10 times of that of the hydrogen-producing bacteria. The advantageous bacterium group not only can degrade the butyric acid with higher concentration, but also can effectively improve the treating efficiency of organic wastewater with high concentration.

This method of restricting methane production in methanogenic bacteria, by the use of the enzyme and coenzyme inhibitors, works during anaerobic reductive dechlorination. Various compounds such as, but not limited to, red yeast rice, vitamin B10 derivatives, and ethanesulfonates are utilized to disrupt these different enzyme and coenzyme systems responsible for the production of methane. This method affects the competition of the methanogen and halo bacteria for the organic hydrogen donors that are injected in the soil and groundwater system during the remediation process.

The present invention provides a system of hydrogen production from microorganisms, wherein a bioreactor provides an environment conducive to the production of hydrogen from Klebsiella oxytoca metabolism and restrictive to the production of methane from methanogens. Klebsiella oxytoca metabolizes organic material at elevated temperatures and pH levels that are detrimental to methanogens. Fixed colonies of Klebsiella oxytoca are contained within the bioreactor, providing a sustained level of hydrogen production. The Klebsiella oxytoca metabolizes organic material in the organic feed material and produces hydrogen in substantially a 1:1 ratio with carbon dioxide. The environment in the bioreactor is preferably maintained between about 3.5 and 6.0 pH.

The invention discloses application of red yeast rice to reduction of methane discharged by ruminant such as sheep. When the red yeast rice is added into ruminant feed to reduce methane discharged by ruminant, the additive amount of the red yeast rice in the daily ration of sheep depends on the average daily feed intake of the sheep, the content ratio of nutrients to lovastatin in the red yeast rice and the ratio of opening loops to closed loops of lovastatin. The red yeast rice is adopted for reducing methane discharged by sheep, and the added red yeast rice naturally containing lovastatin can competitively and powerfully inhibit 3-hydroxy-3-methylglutaryl-coenzyme A reductase and cut off the passage for synthesizing methane bacteria membrane lipid, thereby effectively inhibiting the growth of methane bacteria and eventually reducing methane discharged by ruminant such as sheep.

The invention discloses a biogas fermentation initiator and a preparation method thereof, and belongs to the technical field of biogas preparation through a biological fermentation method. The present problems of slow start, low gas yield efficiency, and no gas yield in the fermentation process are solved. The initiator is composed of the following raw materials: sludge in fishpond, wheat straw, animal manure, ammonium bicarbonate, and bacterium strains; wherein the bacterium strains are bacteroides succinoge-nes, butyrivibrio fibrisolvens, bacillus subtilis, saccharomycetes, methanosarcina, and methanosaeta. The biogas fermentation initiator is applied to biogas fermentation, can greatly shorten the start time (biogas can be produced after one day), and is capable of increasing the biogas yield and biogas generation speed.

The invention provides a method for producing methane through pre-fermenting and strengthening surplus sludge anaerobic digestion. The method comprises the following steps of S1, precipitating and concentrating a sludge to obtain a sludge to be treated; S2, mixing an inoculating mud and the sludge to be treated obtained in the step S1 according to the proportion so as to obtain a fermented mixture, and adjusting pH of the fermented mixture to be acid; S3, mixing the fermented mixture obtained in the step S2 and chelate nickel glycinate according to the proportion, and carrying out hydrolysis fermentation. By using the chelate nickel glycinate under a weak acid condition, the relative abundance of acidogenic fermentation bacterium Clostridia can be improved, the sludge hydrolytic acidification speed is accelerated, and an acidogenic fermentation type is optimized for providing a beneficial substrate for methanogens; meanwhile, through a nickel element dissolved in the sludge, the relative abundance of the methanogens Methanosaeta can be also improved, and the performance for producing the methane through sludge anaerobic digestion is effectively improved.

The invention discloses a microbial agent for reducing sludge. The microbial agent comprises the following components in parts by weight: 8-10 parts of clostridium acetobutylicum, 12-16 parts of bacillus licheniformis, 16-20 parts of bacillus subtilis, 5-7 parts of lactobacillus acidophilus, 5-7 parts of methanosarcina, 3-5 parts of methanosaeta, 6-8 parts of rhodopseudomonas palustris, 4-6 partsof cellulomonas flavigena, 8-10 parts of streptococcus thermophilus, 8-10 parts of alcaligenes faecalis, 3-5 parts of bacillus amyloliquefaciens, 2-4 parts of bacillus nitrosomonas, 1-3 parts of issatchenkia orientalis and 3-5 parts of streptomyces oxalis. The invention also discloses a carrier of the microbial agent for reducing sludge and a preparation method thereof. The carrier is capable of effectively attaching microorganisms and endowing microorganisms with higher activity. Strains are reasonably prepared in the microbial agent disclosed by the invention; the microbial agent is attachedto the carrier for treating sewage; when the method is used for treating sewage, the operation is simple, the treatment effect is excellent and sludge deposition is less; the method is worthy of popularization.

The present invention provides a vaccine pharmaceutical composition for transdermal administration which is safe, usable as a prophylactic or therapeutic agent for cancer or infectious diseases, and capable of safely and effectively inducing a systemic immune response. It can be administered to a human or animal skin, the vaccine pharmaceutical composition including: a lipopolysaccharide or its salt derived from at least one gram-negative bacterium such as Serratia, Lelercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter, as an immunostimulant; and at least one antigen, the vaccine pharmaceutical composition having a mass ratio between the immunostimulant and the antigen, the total mass of the immunostimulant/the total mass of the antigen, of 0.002 to 500.