Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor

A technology for sludge anaerobic fermentation and methanogens, which is applied in the field of sludge recycling, can solve the problems of increased greenhouse gas, large investment in purification and storage, and waste of energy.

Inactive Publication Date: 2009-08-12
JIANGNAN UNIV
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  • Summary
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Problems solved by technology

[0002] The anaerobic digestion process of sludge can be divided into two stages of acid production and methane production. The research on anaerobic digestion of sludge has mostly focused on how to stabilize sludge to the greatest extent and produce more methane for use as However, due to the large investment in methane purification and storage, many sewage plants fail to make good use of it, resulting in energy waste and increased greenhouse gases

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  • Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor
  • Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor

Examples

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Embodiment 1

[0012] The sludge in the natural state was used for fermentation (the sludge was not pretreated and not inoculated with seed sludge). The sludge was taken from the residual sludge of the secondary sedimentation tank of a domestic sewage treatment plant in Wuxi for research, and the experiment was carried out with a shaker flask under experimental conditions. , the liquid volume of the shake bottle is 300mL, BES is added at the beginning of fermentation, and the dosage concentration is 50mmol / L. Compared with the anaerobic fermentation of sludge under natural conditions, the concentration of acetic acid in the fermentation broth can be increased by at least 3 times (from 0.3g / L to 1.2g / L) after adding BES (concentration is 50mmol / L).

Embodiment 2

[0014] The source of sludge and experimental conditions are the same as in Example 1. The sludge is first pretreated with hot alkali (pH 12, 120°C, 30min). Mixed for anaerobic fermentation, after shake flask fermentation, such as figure 1 As shown, the concentration of BES added is 5-20mmol / L, the fermentation time is 13-20d, and the concentration of acetic acid is above 1.2g / L. The optimum concentration of BES was 5mmol / L, and the highest concentration of acetic acid was 1.56g / L after 14 days of fermentation.

Embodiment 3

[0016] Also for the pretreated sludge, under the condition that the concentration of BES added is fixed at 5mmol / L, different initial pHs (6, 7, 8) are preset, such as figure 2 It was shown that when the initial pH was 6, the maximum cumulative concentration of acetic acid in the fermentation broth reached 1.84g / L at 18 days of fermentation.

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Abstract

The present invention provides a method of directional acetic acid production in anaerobic sludge fermentation using methanogenic bacteria-specific inhibitors which belongs to the sludge recycling field. This invention prepares the methanogenic bacteria-specific inhibitor, 2-bromethylsulfonate sodium into solution of certain concentration and adds the solution into the pretreated sludge. When the concentration of 2-bromethylsulfonate sodium is 5-20 mmol / L and the initial pH is 6-8, the maxmium concentration of acetic acid can achieve 1.84g / L after 15-20 day's fermentation, which is more than five times of the yield of acetic acid under natural conditions. The addition of 2-bromethylsulfonate sodium into anaerobic digestion system will inhibit methanogens and facilitates the accumulation of organic acids, especially acetic acid.

Description

technical field [0001] The method for directing acetate production in sludge anaerobic fermentation with a specific inhibitor of methanogens involves a specific inhibitor of methanogens—sodium 2-bromoethanesulfonate, which is injected into the anaerobic digestion system Adding this substance can inhibit methanogens and accumulate organic acids, especially acetic acid, which plays an important role in the directional production of acetic acid in anaerobic fermentation of municipal sludge. It belongs to the field of sludge resource utilization. Background technique [0002] The anaerobic digestion process of sludge can be divided into two stages of acid production and methane production. The research on anaerobic digestion of sludge has mostly focused on how to stabilize sludge to the greatest extent and produce more methane for use as However, due to the large investment in methane purification and storage, many sewage plants fail to make good use of it, resulting in energy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/40C12R1/01
Inventor 陈坚刘和陈艺阳堵国成
Owner JIANGNAN UNIV
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