Composite microbial inoculum used for anaerobic fermentation of kitchen wastes

A compound bacterial agent and anaerobic fermentation technology, which is applied in the field of microorganisms, can solve problems not mentioned above, and achieve the effect of ensuring fermentation efficiency

Active Publication Date: 2013-07-24
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

None of the above published patents mentions the bacterial agent used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0016] Specific embodiment 1: The composite bacterial agent used in the anaerobic fermentation of kitchen waste in this embodiment includes 11 parts by weight of Bacteroides acidogens ( Bacteroides acidifaciens ) DSM 15896 fermentation broth, 30 parts of long-chain fatty acid Syntrophomonas ( Syntrophomonas palmitatica ) DSM 18709 fermentation broth, 20 parts Clostridium sordellii ( Clostridium sordellii ) ATCC 9714 fermentation broth, 24 parts of combined methane mane bacteria ( Methanosaeta concilii ) JCM 10134 fermentation broth and 24 parts of M. henzii ( Methanospirillum hungatei ) DSM 864 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above five strains all reached 10 9 Individuals / mL or more.

[0017] The steps of the preparation method of this composite bacterial agent are as follows:

[0018] (1) Activation of strains: inoculate the preserved Bacteroides oxytogenes DSM 15896 and Clostridium sordellii ATCC 9714 into ...

specific Embodiment approach 2

[0025] Specific embodiment 2: The composite microbial agent for the anaerobic fermentation of kitchen waste in this embodiment includes 10 parts of Bacteroides acidogens DSM 15896 fermentation broth and 28 parts of long-chain fatty acid syntrophomonas DSM 18709 in parts by weight Fermentation broth, 18 parts of Clostridium sordellii ATCC 9714 fermentation broth, 23 parts of joint methane mane bacteria JCM 10134 fermentation broth and 23 parts of Methanospirillum henzii DSM 864 fermentation broth; the effective number of viable bacteria in the fermentation broth of the above five bacteria reached 10 9 Individuals / mL or more.

[0026] The composite bacterial agent of the embodiment was inoculated in a biogas fermenter with a volatile solid (VS) content of 0.8% and food waste as the main raw material with an inoculation amount of 45%, and anaerobic fermentation was carried out at 35°C. One day, the pH value of the fermentation system dropped from the initial 7.0 to 6.2, and then...

specific Embodiment approach 3

[0028] Specific embodiment three: The composite microbial agent for anaerobic fermentation of kitchen waste according to this embodiment includes 8 parts of Bacteroides acidogens DSM 15896 fermentation broth, 25 parts of long-chain fatty acid syntrophomonas DSM 18709 Fermentation broth, 15 parts of Clostridium sordellii ATCC 9714 fermentation broth, 20 parts of joint methane mane bacteria JCM 10134 fermentation broth and 22 parts of Methanospirillum henzii DSM 864 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above five bacteria reached 10 9 Individuals / mL or more.

[0029] The composite bacterial agent of the embodiment was inoculated in a biogas fermenter with a volatile solid (VS) content of 1.3% and food waste as the main raw material with an inoculation amount of 50%, and anaerobic fermentation was carried out at 35°C. One day, the pH value of the fermentation system dropped from the initial 7.0 to 6.1, and then stabilized a...

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PUM

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Abstract

The invention discloses a composite microbial inoculum used for the anaerobic fermentation of kitchen wastes, belongs to the field of micro-organisms, and solves the problem of the lack of a microbial inoculum suitable for performing anaerobic fermentation by taking kitchen wastes as a main raw material at present. The composite microbial inoculum comprises, in parts by weight, 8-15 parts of acid production bacteroides DSM 15896 fermentation liquor, 25-35 parts of long-chain fatty acid syntrophic aeromonas DSM 18709 fermentation liquor, 15-25 parts of clostridium sordellii ATCC9714 fermentation liquor, 20-28 parts of combined methanosaeta JCM10134 fermentation liquor and 22-27 parts of methanospirllum hungatei DSM864 fermentation liquor, wherein the effective viable counts of the fermentation liquors of the five bacteria aforementioned achieve more than 10<9> per millilitre respectively. The composite microbial inoculum disclosed by the invention is suitable for an anaerobic fermentation process taking the kitchen wastes as the main raw material, and capable of playing the roles of stabilizing the pH value of a fermentation system, promoting to bring forward the peak of methanogenesis, and increasing the total amount of methanogenesis after being inoculated in the fermentation system.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a composite bacterial agent made from a variety of microbial fermentation liquids. Background technique [0002] At present, the total amount of municipal solid waste (municipal solid waste, MSW) in the world is increasing year by year, and a considerable part of it is catering waste. The annual production of kitchen waste in large and medium-sized cities in my country has reached 60 million tons. Food waste is perishable, smelly, acidified and easy to become the source of infection of viruses, pathogenic bacteria and pathogenic microorganisms under normal temperature conditions. Therefore, it should be treated scientifically and harmlessly, otherwise it will cause harm to the environment and human health. However, because food waste contains organic substances such as starch, dietary fiber, protein, and lipid, and is also rich in nitrogen, phosphorus, potassium, calciu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P5/02C12R1/145C12R1/01
CPCY02E50/30
Inventor 王彦杰毕少杰王伟东殷奎德晏磊高亚梅刘佳
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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