108 results about "Klebsiella oxytoca" patented technology

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam, or a pharmaceutically acceptable salt thereof, delivered as an aerosol or dry powder formulation.

Provided are compositions for attracting and / or stimulating oviposition. The compositions comprise a suitable carrier and a bacterium capable of producing nonanoic acid, tetradecanoic acid, or methyl tetradecanoate; Bacillus thuringiensis; Lactococcus lactis; Klebsiella oxytoca; Shigella dysenteriae; Brevundimonas vesicularis; a supernatant of a culture of any of the these bacteria; nonanoic acid; tetradecanoic acid; or methyl tetradecanoate, or any combination thereof, at a concentration effective to attract the mosquito to a target. The compositions may be comprised within a kit or trap. Also provided are methods of attracting mosquitoes to a target.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam lysinate delivered as an aerosol or dry powder formulation.

The present invention provides a gene chip for detecting common pathogen in dairy, which comprises a solid-phase vector and an oligonucleotide probe fixed on the solid-phase vector, wherein the oligonucleotide probe includes DNA fragment or its complementary DNA or RNA sequence selected from E. sakazakii, streptococcus pyogenes, staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Listeria monocytogenes, salmonella and 16S-23S rDNA intergenic spacer region of Bacillus cereus, as well as ipaH gene of Shigella or gyrB gene of citrobacter freundii. The invention further provides a reagent box which uses the gene chip to detect pathogen in dairy. The gene chip and the reagent box of the invention for detecting pathogen in dairy are easy to operate, highly precise and greatly repeatable.

The invention discloses a microorganism bacterium agent and a preparation method thereof, and relates to a composite bacterium agent and a preparation method thereof. The composite bacterium agent can be used for efficiently removing a large amount of COD (chemical oxygen demand), ammonia and nitrogen in leather wastewater. The composite bacterium agent is prepared from mycobacterium, microbacterium, acinetobacter, pichia pastoris, lactobacillus, klebsiella oxytoca and a liquid culture medium. The preparation method comprises the following steps of 1, activating the bacteria strains of raw materials; 2, enriching and culturing; 3, mixing the bacterium strains according to a weight ratio, and adding into the liquid culture medium, so as to obtain the composite bacterium agent. The composite bacterium agent has the advantages that the biological activity is high, the growth speed is high, the leather wastewater can be treated under the low temperature condition, the removal rate of organic matter can be obviously increased, the contents of COD, ammonia and nitrogen in the leather wastewater are effectively decreased, and the effect is obvious.

The present invention provides an apparatus and method for the production of hydrogen based on the capture of metabolic by-products of hydrogen-producing microbacteria, in which a bioreactor is maintained in an environment conducive to the growth of hydrogen-producing microbacteria and the production of hydrogen and at the same time is restrictive to the growth of undesirable microorganisms such as methanogens and the production of methane. The present invention utilizes concentrated growth of hydrogen-producing microbacteria such as Klebsiella oxytoca. The invention provides a simple and cost-effective way to produce hydrogen by selectively harnessing hydrogen-producing microbacteria utilizing glucose-based solutions while substantially eliminating methane-producing microbacteria.

The invention belongs to the field of environmental biotechnology and particularly relates to dissimilatory iron reducing bacteria and an application thereof. The dissimilatory iron reducing bacteria provided by the invention is Klebsiella oxytoca IMFRCUG-1 which is collected in the China Center for Type Culture Collection on May 27th, 2015, with a collection number CCTCC NO:M2015332. The Klebsiella oxytoca IMFRCUG-1 provided by the invention has a relatively strong reducing function for dissimilatory reduction of Fe(III) and can effectively reduce the Fe(III) in ferric citrate and montmorillonite. Through the invention, the application research idea in the functions of Klebsiella oxytoca is widened, a new material is provided for the control of environmental pollutants by the dissimilatory iron reducing bacteria, and the Klebsiella oxytoca has relatively high value in practical application.

The invention provides a composite bacterial agent and a method for treating kitchen waste through the composite bacterial agent and relates to the technical field of kitchen waste treatment. The technical problem of low degradation efficiency for the kitchen waste in the prior art is solved. The composite bacterial agent comprises IrPex Cacteus, rumen microorganisms, bacillus subtilis, bacillus cereus, bacillus stearothermophilus, klebsiella oxytoca, bacillus licheniformis and aspergillus niger. By means of the composite bacterial agent and a treatment device and method, 90% or above of organic waste in the kitchen waste can be degraded, and the treatment efficiency is high.

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of isolated bacteria namely, Aeromonas hydrophila, Pseudomonas aeruginosa, Yersinia enterocolitica, Serratia liquefaciens, Pseudomonas fluoresces, Enterobacter cloaca, Klebsiella oxytoca, Citrobacter amalonaticus and Enterobacter sakazaki. The formulated microbial consortium is immobilized on charged nylon membrane. The said immobilized microbial consortium is attached to dissolved oxygen probe for the preparation of electrode assembly. The prepared electrode assembly is used for rapid and reliable BOD estimation. The prepared electrode assembly is used for monitoring of BOD load of synthetic samples such as Glucose-Glutamic acid (GGA) used as a reference standard in BOD analysis and industrial effluents; covering a range from low to high biodegradable organic matter.

The invention discloses an acid-producing klebsiella oxytoca MOW-02-05, a selection method and an application of the acid-producing Klebsiella oxytoca MOW-02-05, wherein the acid-producing klebsiella oxytoca MOW-02-05 is isolated from an intermittent anaerobic sludge reactor and can efficiently degrade dyes and organisms to produce hydrogen; and the klebsiella oxytoca MOW-02-05 is used for decolorizing azo dyes and biologically produce the hydrogen from organic carbon resources. The strain is identified as klebsiella oxytoca MOW-02-05 and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5237. The strain obtained by the invention can solve a problem caused by acidifying the reactor when a conventional anaerobic reactor treats azo dye wastewater, and is suitable for biological treatment of printing and dyeing dye wastewater.

The invention discloses a Klebsiella oxytoca strain and its application in bioelectricity production. The strain is characterized in that it is Klebsiella oxytoca Z6, which is preserved in China Center for Type Culture Collection (called CCTCC for short) on November 6, 2012 and has a preservation number of CCTCC NO: M 2012446. The strain can transmit electrons to an extracellular electron acceptor under an anaerobic condition. When the strain is inoculated into a microbial fuel cell, it can degrade organic matters and generate electricity simultaneously. Compared with other strains, the Klebsiella oxytoca Z6 strain not only has very strong electrochemical activity, but also can make use of various organic matters as the sole carbon source for electricity generation. Based on the above characteristics, the strain has very good application in environmental pollution remediation and bio-energy recovery.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention discloses klebsiella oxytoca. The classification name is klebsiella oxytoca, the strain number is LDS17, the klebsiella oxytoca is collected in the China Center for Type Culture Collection, the strain collection number is CCTCC NO: M 2017583, and the collection date is October 16, 2017. The strain can grow normally in a culture medium with 1-aminocyclopropane-1-carboxylic acid (ACC)as a sole nitrogen source through enrichment culture, and has relatively strong ACC deaminase activity and other growth promoting properties (IAA and siderophore production, HCN production, NH3 production, nitrogen fixation and phosphorus solubilization); after a potted young seedling of the codonopsis is inoculated with a microbial agent produced by the strain, and results show that the microbialagent can significantly promote the growth and the development thereof; inoculation of the strain LDS17 under drought stress effectively promotes the growth and the drought resistance of the codonopsis. Therefore, an excellent strain resource can be provided for developing a special microbial fertilizer for the codonopsis in the future.

The invention discloses a preparation method of a microbial flocculant. The preparation method comprises the following steps: culturing a Klebsiella oxytoca GS-4-08 strain in a fermentation culture solution; centrifuging the fermentation culture solution so as to obtain a supernatant, precipitating the supernatant with the addition of ethanol, and collecting a precipitate; dissolving the precipitate in pure water, dialyzing and freeze-drying so as to obtain the microbial flocculant in powder form. The invention also discloses application of the microbial flocculant in iron-containing acid wastewater treatment. The invention also discloses application of the Klebsiella oxytoca GS-4-08 strain in preparing the microbial flocculant. The microbial flocculant disclosed by the invention has the beneficial effects that the microbial flocculant is relatively high in yield, good in flocculating effect and broad in treatment range, and the microbial flocculant is capable of achieving good settling and flocculating effects on iron-containing acid wastewater; and the method is simple to operate and cheap in raw material, and the method is capable of significantly saving dosage.

The invention discloses a ribitol dehydrogenase (RDH) derived from Klebsiella oxytoca G4A4CGMCC No.7662, and realizes expression thereof in Escherichia coli. The experiment proves that the enzyme can realize the biotransformation production of allitol, and D-allulose can be converted into the functional rare sugar alcohol (allitol) with high conversion rate (96% or above at most) and less pollution; and an NADH (reduced nicotinamide adenine dinucleotide) regeneration system is added in the conversion process, and the NAD (nicotinamide adenine dinucleotide) consumption is low, thereby greatly lowering the cost. The allitol can be used in multiple fields such as preparation of pharmaceuticals (such as diabetes treatment medicaments and the like), health products and other raw materials of rare sugar and the like, and has wide application prospects.

The invention discloses a Klebsiella oxytoca strain, which is collected in China Center for Type Culture Collection (CCTCC) and has the collection number of CCTCC M2011350. The strain can be applied to degrading triazophos pesticide residue, for example, the strain can be prepared into a triazophos pesticide residue degrading bacterium. The bacterium is bacterium suspension mainly prepared from the strain and a basic liquid nutrient medium; and a preparation method for the bacterium comprises the following steps of: culturing the preserved strain by using a plating method until a single colony appears; inoculating the single colony into a seed culture bottle, and culturing by using a seed medium until an exponential phase; inoculating into a production bottle, and culturing by using a production medium until the exponential phase, wherein the inoculating amount of nutrient solution is not less than 1 percent of the total volume of the production medium for the strain; and taking out of the bottle and filling. The invention has the advantages of low production cost, high pesticide removing effect, no toxin, environment friendliness and the like.

The invention discloses a TRFIA of Klebsiella oxytoca, relating to detection of pathogenicbacteria. The invention aims at providing a simple, convenient and rapid TRFIA of Klebsiella oxytoca with high sensitivity and strong specificity. The method comprises the steps of: microbially verifying the Klebsiella oxytoca strains; preparing a Klebsiella oxytoca antibody; performing Eu3+ marking and purifying of IgG; and creating a Klebsiella oxytoca TRFIA detection method. The sensitivity is 1.0x10<5> cfu / pore, which is higher than that of a fluorescence anti-body method. If the fluorescence anti-body method is used for performing entire pathogenicbacteria thallus detection, the fluorescence strength is necessary for visual observation by a microscope, so the subjectivity is large; besides, the detection sensitivity is low resulting from the interference of background fluorescence, and a specific sensitivity value cannot be figured out. As shown in the detection of CPS values of Klebsiella oxytoca with different concentrations by the TRFIA method, the method has the advantages of wide range and capability of quantitative detection.

The invention relates to the technical field of microorganisms, and discloses klebsiella oxytoca and an application thereof. The preservation number of the klebsiella oxytoca is CGMCC No.17360. The invention provides the klebsiella oxytoca which has the effects of dissolving phosphorus, dissolving potassium, resisting salt and the like at the same time. The strain can convert indissolvable phosphorus and potassium elements into rapidly available phosphorus and rapidly available potassium elements of which the state can be directly utilized by plants; and besides, the strain has the effects offixing nitrogen and resisting diseases, the applying quantity of fertilizers is reduced, and besides, the crop yield is increased. The klebsiella oxytoca can be applied to preparation of high-efficiency microbial preparation, has the advantages of being low in production cost, simple to operate, free from secondary pollution to soil and capable of improving crumb structure of soil, can be subjected to field planting in ordinary soil and can be adapted to alkaline soil environment.

The invention discloses a plant growth-promoting rhizobacterium SXH-3. The plant growth-promoting rhizobacterium is classified and named as Klebsiella oxytoca SXH-3 and is preserved in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 6298. The Klebsiella oxytoca SXH-3 can synthesize heteroauxin and Siderophore and has an effect on dissolving phosphorus, so the Klebsiella oxytoca SXH-3 can provide plants with heteroauxin and Siderophore and promote absorption of phosphorus by the plants, thereby promoting growth of the plants. The Klebsiella oxytoca SXH-3 has a substantial promotion effect on plant height, fresh weight and dry weight of leek.

The invention discloses a method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues with xylose extracted from corncobs and belongs to the technical field of biochemical engineering. The method includes the steps of using the corncob residues as a carbon source and carbamide as a nitrogen source, using klebsiella pneumoniae CICC 10011, klebsiella oxytoca CICC 21518, bacillus polymyxa CICC 10010, bacillus cloacae CICC 10014 and a mixed bacterium solution to perform the simultaneous saccharification and fermentation, and enabling the weight of the 2,3-butanediol and acetoin to reach 19.59%-36.13% of the dry weight of the corncob residues. According to the method of preparing 2,3-butanediol by the simultaneous saccharification and fermentation of the corncob residues, the corncob residues are used as the carbon source, so that production cost is effectively reduced, high value utilization of resources is achieved, and huge economic benefits and social benefits are achieved.

The invention provides a multifunctional agricultural soil remediation bacterium agent as well as a preparation method and application thereof, and relates to a bacterium agent as well as a preparation method and application thereof, aiming at solving the problems of an existing microorganism degradation method of DBP (Dibutyl Phthalate) that microorganisms are easily influenced by soil environment factors including indigenous microorganism competition, soil temperature and the like, relatively high bioactivity is difficult to keep and the function is relatively single. The bacterium agent isprepared from a microorganism bacterium agent A and a DBP degradation bacterium fermentation solution, wherein the microorganism bacterium agent A is prepared from a sphingobium fermentation solution,a bacillus mucilaginosus fermentation solution and a klebsiella oxytoca fermentation solution. The method comprises the following steps: preparing the microorganism bacterium agent A; preparing the DBP degradation bacterium fermentation solution; mixing the DBP degradation bacterium fermentation solution with the microorganism bacterium agent A to obtain the multifunctional agricultural soil remediation bacterium agent. The bacterium agent provided by the invention can keep relatively high biological remediation activity in a soil environment for a long term, and the remediation efficiency ofDBP pollution is effectively improved; when DBP polluted soil is repaired, the fertility of the polluted soil also can be improved. The multifunctional agricultural soil remediation bacterium agent is used for the field of soil remediation.

The invention discloses a growth-promoting rhizobacteria SXH-2 with a categorical name of klebsiella oxytoca SXH-2. The strain is collected in China General Microbiological Culture Collection Center with a collection number of CGMCC No. 6297. The strain can synthesize indole acetic acid and siderophore, and has a phosphate-solubilizing effect. Therefore, the strain assists in providing indole acetic acid and iron element for plants, and assists in promoting phosphorus absorption. The strain assists in promoting plant growth. The strain has substantial promoting effect upon plant height, fresh weight and dry weight of leeks.

The invention discloses a bacterium and a constructing method and application thereof. The bacterium features co-operation of 1,3-propylene glycol and L-lactic acid. Further, the bacterium is acid-producing Klebsiella oxytoca and includes acid-producing Klebsiella oxytoca PLL CCTCC M2016186. The constructing method of the bacterium refers to genetic engineering modification for wild fungi. The bacterium and the constructing method and application thereof have the advantages that it is possible to co-produce 1,3-propylene glycol and L-lactic acid by fermenting the bacterium, both the two products are high in molar conversion rate and concentration with fewer types of byproducts and low byproduct concentration, product extraction process can be simplified, efficient biological production of 1,3-propylene glycol and L-lactic acid can be achieved, and the bacterium has promising industrial application prospect.

The present invention provides a method of hydrogen production from microorganisms, wherein a bioreactor provides an environment conducive to the production of hydrogen from Klebsiella oxytoca and restrictive to the production of methane from methanogens. Klebsiella oxytoca metabolizes organic material at elevated temperatures and pH levels that are detrimental to methanogens. Particularly, the environment in the bioreactor is preferably maintained between about 3.5 and 6.0 pH. Further, the Klebsiella oxytoca metabolizes organic material in the organic feed material and produces hydrogen in substantially a 1:1 ratio with carbon dioxide.

The invention relates to a soybean slower-growing rhizobium with broad-spectrum nodulation characteristics, application thereof and a composite rhizobium agent prepared from the same, and belongs to the technical field of agricultural microorganisms. In order to solve the problems that the soybean nodule bacteria are low in nodulation rate and poor in nitrogen fixing effect, the invention providesa Japanese Bradyrhizobium japonicum HF25, the preservation number is CGMCC No.14857, the bacterial strain has high matching affinity, nodulation competitiveness and nodulation and nitrogen fixation capacity. The invention further provides the composite rhizobium agent which is prepared from Japanese Bradyrhizobium japonicum HF25 and Klebsiella oxytoca ASP-15, two kinds of bacteria are compounded,and the nodulation and nitrogen fixation capacity is significantly improved; the composite rhizobium agent is applied to soybean planting, compared with soybeans which are not applied with a microbial agent, the number of soybean nodules is increased by 170%, the number of pods per plant is increased by 37.3%, and the hundred-grain weight of soybeans is increased by 5.5%.

A biodegradation process for the organophosphonate product of Sarin (O-isopropyl methylphosphonofluoridate) hydrolysis, i.e., isopropylmethylphosphonate (IMPA). This process provides a feasible biodegradation demilitarization alternative to Sarin incineration. Public opposition of nerve agent incineration is widespread, and alternative methods are sought to help the U.S. Army meet the 2007 demilitarization deadline imposed by the Chemical Weapons Convention. This process uses a two-step approach to IMPA biodegradation. In the first step, a concentrated IMPA solution is used as the sole nutritional carbon and phosphorus source for microbial cultures. The second step involves diluting the culture and adding an inexpensive carbon source to encourage bacterial phosphate assimilation. The biodegradation typically involves a consortium of microorganisms comprising Methylobacterium radiotolerans GB21, Agrobacterium tumefaciens GB2GA, Klebsiella oxytoca GB2CS, GB272, Aureobacterium sp. GB2 and three bacterial isolates belonging to the same species GB23, GB272, and GB292.

The invention discloses a new stain of Klebsiella oxytoca G4A4 CGMCC No.7662 and application thereof in allitol production, that is the screening bacterial stain of Klebsiella oxytoca G4A4 is prepared into resting cell and is used for allitol production, thereby compensating for the insufficiency of the prior art. The bacterial stain of Klebsiella oxytoca G4A4 has ribose ribitol dehydrogenase, which has a wider substrate spectrum, and under the anaerobic condition, D-psicose can be reduced to allitol. The bacterial stain of Klebsiella oxytoca G4A4 can be applied to the allitol production with D-psicose as substrate, and simple medium can be used for rapid growth and large scale enlarging culture, and the developed allitol production function of bacterial stain of Klebsiella oxytoca G4A4 has a wide application prospect.

This invention consists of a consortium of plant-growth promoting microorganisms including Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae and Klebsiella oxytoca. The bacterial consortium along with a vehicle suitable for agricultural application, form a biofertilizer useful to enhance agricultural yield when applied to cultivation plants. The biofertilizer described present a long shelf life and preserve a high cell density along the time.