19 results about "Haemophilus influenzae" patented technology

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which mainly utilizes a multiple fluorescent PCR analysis method to detect related pathogens, so as to simultaneously realize detection of coronavirus, bordetella pertussis, group A streptococcus, parainfluenza virus, streptococcus pneumoniae, haemophilus influenzae and group B streptococcus in a single-tube reaction system. Or detecting and distinguishing at least four of chlamydia pneumoniae. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention provides a kit and a method for simultaneously detecting 11 pathogens of a urogenital system and application, and belongs to the field of molecular biology. The primer group is used for detecting urogenital tract pathogens, and the urogenital tract pathogens comprise ureaplasma urealyticum (UU), chlamydia trachomatis (CT), mycoplasma genitalium (MG), ureaplasma parvum (UP), mycoplasma hominis (MH), adenovirus (ADV), trichomonas vaginalis (TV), herpes simplex virus type I (HSV-I), herpes simplex virus type II (HSV-II), haemophilus influenzae (HI) and neisseria gonorrhoeae (NG). The primer group is used for multi-joint detection of non-gonococcal urethritis related pathogens, has high specificity and sensitivity, is adaptive to non-invasive urine specimens, and supports home self-sampling. The kit is used for clinical diagnosis and can quickly judge pathogen types. Precise medication is performed based on a detection result, the healing time of a patient is shortened, the burden of a doctor is relieved, and meanwhile the pathogen transmission risk is reduced.

The invention belongs to the technical field of bacterium detection, and discloses a multiple PCR primer for simultaneously detecting eight respiratory tract pathogenic bacteria and application thereof. On the basis of a Luminex liquid phase chip technology, specific primers are designed aiming at eight common respiratory tract infection pathogenic bacteria including pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, stenotrophomonas maltophilia, haemophilus influenzae and escherichia coli; the multiple PCR primers which are high in amplification efficiency, good in specificity and suitable for simultaneously detecting the eight respiratory tract pathogenic bacteria are screened out, the multiple PCR system and microsphere hybridization conditions are continuously optimized, the multiple PCR method for the eight respiratory tract pathogenic bacteria is successfully constructed by applying the Luminex xTAG technology, and the multiple PCR method can be applied to rapid detection of multiple pathogenic microorganisms.

The present application relates to the field of biological medicine, in particular to a sensitizing agent for constructing a neutrophilic asthma animal model, a construction method and application. The present application uses Haemophilus influenzae outer membrane vesicles and ovalbumin as a sensitizing agent to construct a neutrophilic asthma animal model, and the test results show that the level of neutrophils in the blood and lung lavage fluid of mice is significantly increased compared with the treatment of ovalbumin as a sensitizing agent alone. The constructed mouse neutrophilic asthma model can be used for the research on the mechanism of clinical neutrophilic asthma and the development of neutrophilic asthma drugs.

The present application relates to the field of biochemistry, and discloses a multiple primer set and a kit for detecting pathogens of acute infection of the lower respiratory tract, including primer pairs for detecting Mycoplasma pneumoniae, primer pairs for detecting Chlamydia pneumoniae, primer pairs for detecting Staphylococcus aureus, primer pairs for detecting Haemophilus influenzae, primer pairs for detecting Streptococcus pneumoniae, and primer pairs for detecting Klebsiella pneumoniae. The multiple primer set for detecting pathogens of acute infection of the lower respiratory tract of the present application can realize simultaneous amplification and detection of 6 targets, and is suitable for screening of clinical cases of acute lower respiratory tract infection.

The invention relates to the technical field of molecular diagnosis and early screening of tumors, and particularly discloses a plasma circulating microbial marker for early screening of lung cancer and application of the plasma circulating microbial marker. The marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and haemophilus parainfluenza (Haemophilus parainfluenza), and the marker comprises the following components: staphylococcus aureus, fusobacterium nucleatum, klebsiella pneumoniae, alternaria simplex, legionella pneumophila, neisseria mucosa, Ralstonia petii, pasteurella multocida, and Haemophilus parainfluenza (Haemophilus parainfluenza). According to the method, nine characteristic microbial markers are detected in combination with a low Pass WGS technology, a high-precision diagnosis model is constructed through a machine learning algorithm, the established diagnosis model has excellent stability and clinical applicability, efficient recognition of early lung cancer can be achieved, and the detection cost can be greatly reduced.

The invention discloses a pneumonia risk early warning method and system based on SNP site analysis. The method comprises the following steps: firstly, through comparative genomics analysis, respectively screening out two sets of core SNP site combinations which are obviously related to inseparable haemophilus influenzae (NTHI) pathogenic transformation and chronic disease progression; then, based on genotype data of the two sets of site combinations, a classification data set is independently constructed, and a machine learning algorithm is used to train and optimize to generate a health risk early warning model and a chronic risk assessment model. The trained model is utilized to analyze a to-be-tested sample, and the pathogenic risk probability and the chronic risk probability of the to-be-tested sample can be output respectively; the system comprises a data preprocessing and site screening module, a model construction module and an early warning analysis module. The method realizes staged and accurate quantitative evaluation of the NTHI pneumonia risk, and is suitable for large-scale screening and accurate prevention and control.

This invention relates to the fields of bioinformatics and data processing technology, and provides a draft data quality correction and optimization system based on the complete genome benchmark of Haemophilus influenzae. The system includes: an automated data acquisition module configured to automatically acquire the dataset and metadata of the target microorganism from a public database; a dynamic benchmark construction module configured to construct a standard feature vector based on the dataset and metadata of the target microorganism; an interference calculation module configured to calculate the fragmentation interference coefficient K of the dataset relative to the standard feature vector; and an adaptive correction and optimization module configured to correct the confidence level of gene detection results according to the interference coefficient K and output an optimized dataset with weight labels. This invention solves the problems of data waste and false negatives caused by hard threshold filtering in existing technologies, and realizes the effective utilization and accurate correction of low-quality genomic data.

The invention relates to the technical field of vaccine preparation, in particular to a GnRH castration vaccine based on a haemophilus influenzae D protein carrier and a preparation method. The amino acid sequence of the recombinant PD-GnRH fusion protein disclosed by the invention is as shown in SEQ ID NO. 1; the recombinant PD-GnRH fusion protein comprises a haemophilus influenzae D protein and a mammal GnRH sequence. The fusion protein castration vaccine is developed by adopting a PD and GnRH protein recombination mode. Compared with other castration vaccines, the vaccine provided by the invention reduces animal stress, is more convenient to use, has the advantages of safety, convenience, mild action effect and the like, can be directly used for animal intramuscular injection, is simple and easy to operate, achieves a more ideal effect on control of animal reproductive organ development and reproductive function, and can be widely applied to animal intramuscular injection. And the development of an animal castration technology is further promoted.

The present invention relates to a multi valent vaccine composition comprising antigens against Diphtheria toxoid (DT), Tetanus toxoid (TT), Whole-cell pertussis (wP) / Acellular Pertussis (aP), Polio / Inactivated Poliomyelitis (IPV), Hepatitis (Hep) and Haemophilus influenzae (Hib) wherein Haemophilus influenzae (Hib) conjugated to Protein D or protein D fragment as a carrier protein. This invention also relates to processes for their preparation and immunogenic compositions comprising them, wherein protein D induces protective responses against invasive infections caused by Haemophilus influenzae (Hib).

The present invention provides multiple fluorescent probe primers and kits for simultaneously detecting multiple pathogens of central nervous system infection. Amplification primers and different fluorescent labeled Taqman fluorescent probes are designed for 9 pathogenic bacteria, including Listeria monocytogenes, Haemophilus influenzae, Streptococcus pneumoniae, Streptococcus agalactiae, Neisseria meningitidis, coagulase-negative Staphylococcus, Staphylococcus aureus, Acinetobacter baumannii and Klebsiella pneumoniae. At the same time, human-derived internal references are added as internal quality control of the kit, so that the reagent can distinguish 3 kinds of target targets and endogenous internal reference genes in a single tube. In addition, probes and primers for coagulase-negative Staphylococci in cerebrospinal fluid can effectively detect Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus capitis, Staphylococcus saprophyticus, and Staphylococcus hemolyticus, and can be effectively distinguished from coagulase-positive Staphylococci such as Staphylococcus aureus, Staphylococcus dolphinus, Staphylococcus suis, etc.

The invention belongs to the technical field of tremella sanguinea culture and cultivation, and particularly discloses a tremella sanguinea strain cultivation and bottle cultivation method which comprises the following steps: separating from a tremella sanguinea sporocarp based on a spore ejection method and carrying out purification culture to obtain tremella sanguinea basidiospore; separating from auricularia sanguinea wood tissues, purifying and culturing to obtain associated fungus hyphae; pairing the tremella sanguinea basidiospores in a culture medium based on a spore pairing method to obtain tremella sanguinea hyphae; respectively inoculating tremella sanguinea hyphae and associated fungus hyphae into a culture medium for light-proof activation culture to respectively obtain cultivation mother strains; the method comprises the following steps: putting a culture material into a wide-mouth bottle, compacting, punching an inoculation hole in the center, covering with a bottle cap with a breathable film, sterilizing under high pressure, inoculating a mixed cultivation mother strain under an aseptic condition, carrying out dark culture until hyphae grow full of the bottle, and carrying out fruiting management to obtain a tremella sanguinea fruiting body. The method provided by the invention has the advantages of early fruiting time, short growth cycle, capability of fruiting by substitute cultivation, stable strain, capability of obtaining pure tremella sanguinea hyphae and the like.

Compositions and methods are provided for reducing the transmission of at least one pathogen including S. pneumoniae through administration to subjects of immunogenic compositions comprising a recombinant, live attenuated S. pneumoniae expressing on its cell surface at least one heterologous immunogenic protein, or an immunogenic fragment or variant thereof. The immunogenic compositions also serve to reduce the incidence rate of at least one disease, such as acute otitis media, caused by at least one pathogen. Examples of heterologous immunogenic proteins, or immunogenic fragments or variants thereof, include H. influenzae protein D and M. catarrhalis UspA polypeptide. The expressed heterologous immunogenic protein, or an immunogenic fragment or variant thereof, is modified with a surface anchor moiety for anchoring at the cell surface of S. pneumoniae.

The invention belongs to the field of biological detection, and relates to a primer probe combination, a kit and a detection method for quantitatively detecting haemophilus influenzae, primers comprise an upstream primer for detecting haemophilus influenzae, a downstream primer for detecting haemophilus influenzae, an internal control upstream primer and an internal control downstream primer, the probe comprises a haemophilus influenzae detection probe and an internal control gene probe; the nucleotide sequence of the upstream primer for detecting haemophilus influenzae is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream primer for detecting haemophilus influenzae is as shown in SEQ ID NO: 2; the nucleotide sequence of the internal control upstream primer is as shown in SEQ ID NO: 3, and the nucleotide sequence of the internal control downstream primer is as shown in SEQ ID NO: 4; the nucleotide sequence of the haemophilus influenzae detection probe is as shown in SEQ ID NO: 5, and the nucleotide sequence of the internal control gene probe is as shown in SEQ ID NO: 6. According to the application, the qualitative and quantitative detection of haemophilus influenzae can be realized at the same time, and the sensitivity and accuracy of haemophilus influenzae detection are improved.

The present application relates to a kind of essential oil composition with the efficacy of improving immunity, belong to daily chemical technology field.The essential oil composition with the efficacy of improving immunity of the present application includes the following mass parts of components:Rowensha leaf essential oil 10-20 parts, eucalyptus essential oil 15-30 parts, green flower of guettarda speciosa essential oil 10-20 parts, guaiac essential oil 5-10 parts, cedar essential oil 5-20 parts.The essential oil composition with the efficacy of improving immunity of the present application can significantly reduce the secretion of TNF-alpha, and have certain inhibitory effect on streptococcus pneumoniae, haemophilus influenzae, in addition, also have certain antitussive efficacy, thereby verifying its efficacy of improving immunity, relieving respiratory discomfort.The composition of the present application can be used for preparing massage oil, aromatherapy spray, inhalant, cream, patch and other care products, with good application prospect.

Disclosed are methods, systems, components, and compositions for cell-free synthesis of glycosylated proteins, which may be utilized in vaccines, including anti-bacterial vaccines. The glycosylated proteins may include a bacterial polysaccharide conjugated to a carrier, which may be utilized to generate an immune response in an immunized host against the polysaccharide conjugated to the carrier. Suitable carriers may include but are not limited to Haemophilus influenzae protein D (PD), Neisseria meningitidis porin protein (PorA), Corynebacterium diphtheriae toxin (CRM 197), Clostridium tetani toxin (TT), and Escherichia coli maltose binding protein, and variants thereof.

The invention discloses a streptococcus pneumoniae, mycoplasma pneumoniae and haemophilus influenzae nucleic acid detection kit based on a digital PCR technology. In particular discloses a kit for detecting SP, MP and / or HI, the kit comprises a composition for detecting SP, MP and / or HI, and the composition comprises primers with nucleotide sequences as shown in SEQ ID No.1-6 and probes with nucleotide sequences as shown in SEQ ID No.7-9. The kit, the primer and the probe have good specificity and stability, can effectively avoid a false positive amplification phenomenon, and have high sensitivity, and the lower detection limit can reach 1 copy / microliter; according to the present invention, the droplet digital PCR technology is applied to the SP, MP and / or HI detection method, such that the characteristics of accuracy, specificity, sensitivity, convenience and reliability are provided, and the potential application value is provided in the aspects of SP, MP and HI diagnosis and treatment, and the like.

The present application relates to the technical field of microfluidics, and particularly relates to a kit for rapidly detecting 15 respiratory pathogens and a use method thereof.The kit comprises a plurality of reaction cavities, each of which has a primer group corresponding to 15 respiratory pathogens; the primer group comprises inner primers, outer primers and loop primers for PCR amplification of Mycoplasma pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Haemophilus influenzae, Streptococcus pneumoniae, Escherichia coli, Legionella pneumophila, Burkholderia, Staphylococcus aureus, Corynebacterium striatum, Aspergillus, Pneumocystis jirovecii and Candida albicans.The present application can simultaneously detect 15 respiratory pathogens by adding samples once, avoids adding liquid reagents to multiple detection tubes, reduces the intensity of manual operation and the risk of mismatch.

The invention relates to a clarithromycin derivative containing a quinolone structure as well as a preparation method and application of the clarithromycin derivative. The compound provided by the invention has antibacterial activity equivalent to that of telithromycin in constitutive streptococcus pneumoniae drug-resistant bacteria and streptococcus pyogenes drug-resistant bacteria. And the antibacterial activity on haemophilus influenzae is better than that of Thailimycin. Furthermore, the anti-streptococcus pyogenes 12-206 activity of part of the compounds disclosed by the invention is obviously superior to that of the existing compounds. In addition, the compound provided by the invention not only can act on ribosome, but also can act on topoisomerase, and is a double-target compound at the cellular level. Therefore, the compound disclosed by the invention is not easy to induce bacteria to generate mutation, and the service life of the compound as an antibacterial agent can be prolonged.