The invention discloses application of recombinant
klebsiella pneumoniae in producing 1,3-
propylene glycol and particularly discloses a method for improving the yield of the 1,3-
propylene glycol by using mexF
gene in heterologously-expressed pseudomonase aeruginosa of the
klebsiella pneumoniae and application thereof, and belongs to the technical field of
genetic engineering. According to the method disclosed by the invention, by applying a
genetic engineering method,
plasmid with the mexF
gene in the
pseudomonas aeruginosa is transferred to the
klebsiella pneumoniae for enhancing the transportation efficiency of the klebsiella pneumoniae to short chain
alcohol, so that the yield of the 1,3-
propylene glycol is improved. The yield of the 1,3-propylene glycol reaches as high as 74g / L by carrying out fed-
batch fermentation on arecombinant strain in a 5L
fermentation tank. The method has the advantages that the
transportation capacity of the klebsiella pneumoniae to the 1,3-propylene glycol is successively improved, provides a novel method for improving the yield of the 1,3-propylene glycol and provides a new idea for breeding high-yielding strains of the 1,3 propylene glycol.