Pseudomonas aeruginosa and its application

A technology of Pseudomonas aeruginosa and Pseudomonas aeruginosa, applied in Pseudomonas aeruginosa and its application fields, can solve the problems of poor tolerance of reservoir formation water salinity, difficult growth and reproduction, and lack of adaptability, etc. Achieving social production friendliness, improving oil displacement efficiency, and increasing effective permeability

Active Publication Date: 2015-02-25
PETROCHINA CO LTD
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Pseudomonas aeruginosa screened from the common environment is not adaptable to the reservoir environment, especially the tolerance to the salinity of reservoir formation water is very poor, and it is difficult to grow and reproduce when the salinity exceeds 10g / l. Unable to carry out microbial oil recovery engineering in high salinity reservoirs

Method used

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  • Pseudomonas aeruginosa and its application
  • Pseudomonas aeruginosa and its application
  • Pseudomonas aeruginosa and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Obtaining of Pseudomonas aeruginosa QHH S1-27-2

[0018] Collect oil and water samples from oil production wells in Qinghai Oilfield, pack them in sterile polystyrene bottles, and bring them back to the laboratory.

[0019] (1) Enrichment: Take 10g of oil and water samples and add them to the enrichment medium, put 100mL of fermentation medium into a 250mL Erlenmeyer flask, seal the bottle mouth, 50°C, 120r / min, constant temperature shaking culture for 3 days.

[0020] (2) Blood plate separation

[0021] After the enrichment culture, observe the form of the fermentation broth, draw 0.1mL from the shake flask culture that can disperse the oil into oil droplets to make 10 -5 -10 -7 Gradual dilution, and then use a sterilized applicator to evenly coat the blood plate, and incubate in a constant temperature incubator at 37°C for 2 days. The characteristics of biosurfactants that can dissolve red blood cells are shown as hemolytic circles on blood plates. Therefore, pick...

Embodiment 2

[0031] Deoiling effect of Pseudomonas aeruginosa QHH S1-27-2 on crude oil

[0032] The crude oil and quartz sand in the Huatugou work area of ​​Qinghai Oilfield were mixed at a volume ratio of 1:1, and aged at 37°C for 1 week. Take 20g of dried quartz sand with oil, add 50ml of 3% strain fermentation liquid, add Huatugou formation water to the blank control, and cultivate at a simulated formation temperature of 35°C for 48 hours. After 0.5, 1, 3, 6, 12, 24, and 48 hours, take out and measure the crude oil floating on the liquid surface. The deoiling efficiency of the fermentation broth of the oil-displacing bacterial strain was detected. With the prolongation of the action time of the fermentation liquid of the strain, the oil layer in the beaker where the oil-displacing microbial fermentation liquid was added gradually thickened, and the bottom quartz sand gradually revealed its true color, and the deoiling effect was very significant. It was determined by experiments that 3...

Embodiment 3

[0036] Determination of the adaptability of Pseudomonas aeruginosa QHH S1-27-2 to the environment

[0037] (1) Determination of strain reproductive ability

[0038]After being inoculated at 3% in the culture medium, culture at 35°C with shaking at 60r / min, and measure the OD value every 4h. According to the linear relationship between bacterial concentration and OD value, calculate the bacterial concentration corresponding to different OD values. Pseudomonas aeruginosa QHH S1-27-2 enters the logarithmic growth phase after 8-12 hours, enters the stable phase after about 24-32 hours, and the number of bacteria corresponding to the OD value is greater than 10 7 CFU / mL.

[0039] (2) Determination of the adaptability of strains to temperature

[0040] Design 3 temperature gradients of 30, 35, and 40°C, inoculate the culture medium with 3% inoculum, and measure the OD value after shaking for 28 hours (the number of bacteria in the stable period is relatively constant). The growt...

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Abstract

The invention relates to a Pseudomonas aeruginosa and its application. The microbial preservation number of Pseudomonas aeruginosa QHH S1-27-2 is CCTCC NO:M2012468. The Pseudomonas aeruginosa is applied to high salinity reservoir microbial oil recovery. The Pseudomonas aeruginosa can growth with crude oil as a sole carbon source, and a biological surfactant component in the metabolites of the Pseudomonas aeruginosa can reduce oil-water interfacial tension, so the effective permeability of reservoirs is increased to a certain extent, the oil displacement efficiency of injected water is improved, and the crude oil recovery rate is improved.

Description

technical field [0001] The invention relates to Pseudomonas aeruginosa and its application in the field of biotechnology, in particular to a strain of Pseudomonas aeruginosa producing biosurfactant and its application in microbial oil recovery of high salinity oil reservoirs. Background technique [0002] Petroleum is a non-renewable resource, but 60-70% of remaining oil and residual oil remain in the formation after secondary oil recovery, which has great potential for improving recovery and increasing recoverable reserves. It is estimated that a 1% increase in the oil recovery rate of an oil field can meet the world's oil consumption for 2 to 3 years. Therefore, how to improve the level of tertiary oil recovery technology has important economic value and strategic significance. [0003] Tertiary oil recovery mainly includes physical oil recovery, chemical oil recovery and microbial oil recovery. The physical oil recovery technology mainly injects high-temperature and high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582E21B43/22C12R1/385
CPCC09K8/582C12N1/205C12R2001/385E21B43/16
Inventor 扈福堂张立会周平薛姝雯
Owner PETROCHINA CO LTD
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