6results about How to "Highly conservative" patented technology

The application discloses a city annual water consumption prediction method based on variable point detection and segmented double-layer fusion, relates to the technical field of city water consumption prediction, and comprises the following steps: collecting city water consumption historical data and preprocessing to obtain annual water consumption data; calculating a water consumption growth rate sequence of adjacent years, searching for an optimal abnormality sub-section in combination with an abnormal section length range, and identifying a water consumption time sequence variable point section; a plurality of prediction model sets are constructed; one-step prediction is performed through rolling, prediction errors of normal sections and variable point sections are respectively counted, and two types of global fusion weights are generated; dynamic weights are solved in combination with a k-year rolling error cache and an exponential weighting mechanism, and double-layer weights are fused; whether the target year is in a variable point section or not, parameters are adaptively adjusted, corresponding weights are matched, city water consumption final prediction results are weighted and calculated, and output. The application still has good accuracy and robustness in the variable point section.

This invention discloses a primer-probe combination for detecting multiple types of Lactobacillus and its applications, belonging to the field of biotechnology. The primer-probe combination includes a primer-probe combination for detecting *Lactobacillus casei*, a primer-probe combination for detecting *Lactobacillus paracasei*, and a primer-probe combination for detecting *Lactobacillus rhamnosus*. The primer-probe combination of this invention can be used for both singleton and multiplex PCR detection, and can accurately detect *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*, enabling the authentication of products containing Lactobacillus. This invention provides a more convenient method for detecting *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*.

The application relates to the technical field of voltage compensation control, in particular to a power distribution line dynamic compensation adjustment method for preventing voltage fluctuation. The method comprises the following steps: acquiring voltage deviation degrees and reactive power variation synchronization degrees between each trunk node and each compensation point at each moment; acquiring limit coefficients of each trunk node, and dividing an alternating current power grid area into reactive power compensation coverage sub-areas dominated by each compensation point; calculating line losses of trunk lines in each reactive power compensation coverage sub-area, and acquiring multi-time scale estimation information; acquiring compensation parameters of reactive power compensation devices in each reactive power compensation coverage sub-area, and then correcting compensation capacities of the reactive power compensation devices to dynamically compensate the power distribution line. The application aims to reduce active power loss and voltage drop and improve overall power quality.

The invention belongs to the technical field of biology, and discloses a method for determining a multivalent antigen sequence of an anti-periodontitis red complex, which comprises the following steps: firstly, screening out key virulence factor antigens (FimA-II, RgpA, Msp and BspA) from three core pathogenic bacteria of the red complex: porphyromonas gingivalis, treponema denticola and fusisterone; then, independently cloning each antigen gene to a pUC57 cloning vector, constructing a single antigen recombinant plasmid, and avoiding intergene interference by designing a specific enzyme cutting site; and finally, through plasmid transformation, positive clone screening and double enzyme digestion verification, the plasmid quality is ensured. The obtained single antigen plasmid can be flexibly combined and used, is beneficial to subsequent multivalent vaccine development, and has the advantages of wide antigen coverage, high expression reliability, flexible construction strategy, easiness in industrialization and the like.

The invention discloses a primer probe combination for detecting various bifidobacteria and application of the primer probe combination, and belongs to the field of molecular biological detection. The primer probe combination provided by the invention comprises a primer probe group for detecting bifidobacterium breve, a primer probe group for detecting bifidobacterium bifidum and a primer probe group for detecting bifidobacterium adolescentis. Based on the primer probe combination, the invention respectively develops a single PCR detection method and a triple PCR detection method for detecting the three bifidobacteria. Experimental results show that the detection method provided by the invention can be used for accurately detecting bifidobacterium breve, bifidobacterium bifidum and bifidobacterium adolescentis. According to the invention, a foundation is laid for authenticity identification of products containing bifidobacterium breve, bifidobacterium bifidum and bifidobacterium adolescentis, and a more convenient method is provided for detection of bifidobacterium breve, bifidobacterium bifidum and bifidobacterium adolescentis.

This invention relates to an antibody or antigen-binding fragment thereof targeting a conserved region of the porcine reproductive and respiratory syndrome virus (PRRSV) GP5 protein. The conserved region is selected from amino acids 32–101 and / or 125–199. The antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown in SEQ ID NO: 2; the amino acid sequence of the light chain variable region is shown in SEQ ID NO: 4. This antibody or its antigen-binding fragment comprises a heavy chain and a light chain and can specifically bind to the GP5 protein, and can be used to detect the presence or content of PRRSV GP5 protein in a sample.