100results about How to "Not easy to mutate" patented technology

The invention relates to a method for making landscape plant waste compost inoculum and an application of the same. The method is as follows: bacillus subtilis, trichoderma viride, lactobacillus plantarum, paracoccus denitrification, aspergillus oryzae and saccharomyces cerevisiae respectively occupying 10 to 20 mass portions are inoculated in a solid state fermentation medium so as to carry out cultivation and fermentation in dark sealing for 1 to 3 weeks at a temperature of between 25 and 30 DEG C; one week later, 250 mass portions of saw dust is added and evenly stirred and then cultivation and fermentation are continued; two weeks later, hay is placed to absorb surplus moisture and then is removed, and powdery inoculum can be obtained through drying in the shade for further use. Garden wastes are piled up and then are inoculated with inoculum with the weight occupying 1 to 3 per mill of that of a pile and dipped in warm water at a temperature of between 30 and 40 DEG C; compost is carried out at a temperature of between 55 and 65 DEG C, and pile turning and water replenishing are carried out every 3 to 4 days; then, pile turning and water replenishing are carried out every two weeks so as to keep pile water content of between 50 and 60 percent; thus, compost is completed after three weeks so as to reach to rotten innocuity standards. The strain has simple making and low cost, and can be directly used or bred continuously; moreover, the strain has no variation, no inactivation and long storage period, and the obtained compost can improve soil and increase nutrient utilization rate.

The invention relates to an embedded antigen receptor based on CD30 and application thereof, and particularly relates to a building method of an embedded antigen receptor T (CAR-T) cell technology based on a tumor specific target site CD30 and application thereof in anti-tumor treatment. The embedded antigen receptor is prepared by connecting an antigen binding structural domain, a transmembrane structural domain, a costimulatory signal conducting region and a CD3 zeta signal conducting structural domain in series, wherein the antigen binding structural domain is combined with tumor-surface antigen, and the tumor-surface antigen is CD30. By adopting the embedded antigen receptor, specific gene transformation on a single chain antibody of the tumor-surface antigen CD30 is carried out, and because of the transformed antibody, the binding force of the antigen-antibody is stronger, and the mutation difficultly occurs; compared with other embedded antigen receptors and other tumor antigens, the embedded antigen receptor has a better effect, and the expression quantity of the target site is high, so that an immunization effect of CAR-T cells is enhanced, and a treatment effect on the CAR-T cells is enhanced.

The invention relates to an embedded antigen receptor based on CD22 and application thereof, and particularly relates to a building method of an embedded antigen receptor T (CAR-T) cell technology based on a tumor specific target site CD22 and application thereof in anti-tumor treatment. The embedded antigen receptor is prepared by connecting an antigen binding structural domain, a transmembrane structural domain, a costimulatory signal conducting region and a CD3 zeta signal conducting structural domain in series, wherein the antigen binding structural domain is combined with tumor-surface antigen, and the tumor-surface antigen is CD22. By adopting the embedded antigen receptor, specific gene transformation on a single chain antibody of the tumor-surface antigen CD22 is carried out, and because of the transformed antibody, the binding force of the antigen-antibody is stronger, and the mutation difficultly occurs; compared with other embedded antigen receptors and other tumor antigens, the embedded antigen receptor has a better effect, and the expression quantity of the target site is high, so that an immunization effect of CAR-T cells is enhanced, and a treatment effect on the CAR-T cells is enhanced.

The invention discloses acinetobacter baumannii, and a screening method and an application thereof in degradation of an azo dye Congo red. The stain is named as acinetobacter baumannii YNWH226, is preserved in China general microbiological culture collection center, and has the preservation number of CGMCC No.7922. The invention provides the new acinetobacter baumannii and the application thereof in degradation of the dye. Especially, the acinetobacter baumannii can effectively degrade the azo dye Congo red, has quite good decoloration and mineralization effects on Congo red wastewater, can completely mineralize the Congo red under a simple aerobic condition, and has great significance on governance of dye pollution and study on aerobic degradation of the azo dye.

The invention relates to a chimeric antigen receptor based on CD20, and applications thereof, and more specifically relates to a cell technology construction method of chimeric antigen receptor T (CAR-T) taking tumor specific target spot CD20 as a base, and applications of the chimeric antigen receptor T in treatment of tumor. The chimeric antigen receptor T is composed of an antigen binding domain, a transmembrane domain, a costimulatory signal transduction region, and a CD3 zeta signal transduction domine via series connection; wherein the antigen binding domain is used for binding tumor surface antigens, and the tumor surface antigen is CD20. According to applications, specific gene modification of single-chain antibody of tumor surface antigen CD20 is carried out, the modified antibody is capable of increasing antigen-antibody binding force, mutation is not easily caused. Compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor possesses following advantages: the effect is better, target spot expression quantity is higher, immune effect on CAR-T cells is improved, and treatment effect of CAR-T cells is improved.

The invention relates to a chimeric antigen receptor, and application thereof, specifically to a construction method for a cell technology of a chimeric antigen receptor T (CAR-T) based on a tumor-specific target CD20 and application of the CAR-T to anti-cancer treatment. The chimeric antigen receptor is formed by an antigen binding domain, a transmembrane domain, a costimulatory-signal transduction domain and a CD3 zeta signal transduction structural domain through series connection, wherein the antigen binding domain binds to a tumor surface antigen, and the tumor surface antigen is CD20. The chimeric antigen receptor carries out specific gene modification on a single-chain antibody targeting to the tumor surface antigen CD20, so the modified antibody allows antigen-antibody binding force to be stronger and mutation is hard to occur; and compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor provided by the invention has better effect, a high target expression level and improves the immunization effect and treatment effect of CAR-T cells.

The invention discloses a spine-free zanthoxylum bungeanum cultivation method, which comprises land selecting and preparation, seedling raising, ear cutting and grafting, field planting, cultivation management, and seedling raising of spine-free zanthoxylum bungeanum, wherein a seed stirring agent is used during the seedling raising process, and during the ear cutting and grafting, the scion male parent is soaked with an essence liquid so as to improve the healing and scion survival rate of the joint part. According to the present invention, with the grafting of more than three generations, the spine-free or micro-spine characteristic can be stabilized, and the new grafted seedling has characteristics of high survival rate, strong branches, convenient transplanting and good quality.

The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.

The invention discloses an acid-producing klebsiella oxytoca MOW-02-05, a selection method and an application of the acid-producing Klebsiella oxytoca MOW-02-05, wherein the acid-producing klebsiella oxytoca MOW-02-05 is isolated from an intermittent anaerobic sludge reactor and can efficiently degrade dyes and organisms to produce hydrogen; and the klebsiella oxytoca MOW-02-05 is used for decolorizing azo dyes and biologically produce the hydrogen from organic carbon resources. The strain is identified as klebsiella oxytoca MOW-02-05 and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5237. The strain obtained by the invention can solve a problem caused by acidifying the reactor when a conventional anaerobic reactor treats azo dye wastewater, and is suitable for biological treatment of printing and dyeing dye wastewater.

The invention relates to a levalbuterol hydrochloride orally disintegrating tablet and the preparation method; wherein the levalbuterol hydrochloride orally disintegrating tablet comprises an active pharmaceutical ingredient, the levalbuterol hydrochloride, and pharmaceutical necessities. The pharmaceutical necessities include a disintegrant, a filling agent, a masking agent and a corrective. The raw material ingredients calculated by weight ratio are 0.79-2.5 per cent levalbuterol hydrochloride, 50-60 percent disintegrant, 20-40 per cent filling agent, 1.58-10 per cent masking agent and 10-12 per cent corrective, which are made into the orally disintegrating tablets by direct powder compression process. The tablets of the invention is taken easily without water, disintegrated quickly and takes effect quickly, high in bioavailability, less stimulatory function to gastrointestinal tract, and applicable to prevention and cure of bronchial asthma, asthmatic bronchitis, and bronchospasm of an emphysema patient.

The invention relates to a chimeric antigen receptor based on CD10, and applications thereof, and more specifically relates to a cell technology construction method of chimeric antigen receptor T (CAR-T) taking tumor specific target spot CD10 as a base, and applications of the chimeric antigen receptor T in treatment of tumor. The chimeric antigen receptor T is composed of an antigen binding domain, a transmembrane domain, a costimulatory signal transduction region, and a CD3 zeta signal transduction domine via series connection; wherein the antigen binding domain is used for binding tumor surface antigens, and the tumor surface antigen is CD10. According to applications, specific gene modification of single-chain antibody of tumor surface antigen CD10 is carried out, the modified antibody is capable of increasing antigen-antibody binding force, mutation is not easily caused. Compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor possesses following advantages: the effect is better, target spot expression quantity is higher, immune effect on CAR-T cells is improved, and treatment effect of CAR-T cells is improved.

The invention relates to a proliferation accelerant and application thereof, in particular to a fibroblast proliferation accelerant capable of promoting fibroblast proliferation and fibroblast extractand fibroblast extract freeze-dried powder prepared through fibroblasts. The proliferation accelerant comprises loofah saponin and / or momordica saponin. A loofah saponin and momordica saponin combined inducer is adopted for culture of skin fibroblasts, so that synthesis and secretion of bioactive substances derived from the skin fibroblasts are promoted effectively, content of active ingredientsin cell derivatives is increased, and production efficiency and biologic effect of the skin fibroblast derived cell derivatives.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

The invention discloses a tissue culture rapid propagation method of eucalyptus cloeziana. The method adopts an excellent single plant of an excellent eucalyptus cloeziana family, which is cut down and promoted to germinate, a bud serves as an explant, a stem section with a resting bud induces a sterile bud in vitro, the sterile bud is obtained without a callus seedling path, the stem section of the explant directly grows a side bud or an adventitious bud, and the sterile bud is subjected to subculture enrichment culture, rooting culture and rooting seedling transplanting to obtain a regenerated plant. The tissue culture rapid propagation method of eucalyptus cloeziana is lower in operation difficulty, stable in the growth of a sterile bud propagation system and good in repeatability and can be used for scale expanding propagation repeatedly, after the rooting culture of cut buds, the rooting rate is higher, the popularization value is realized, rooting seedlings can survive after transplanting, seedlings normally grow, the obtained regenerated plant can robustly grow in a large field, a survival rate of afforestation is more than 95% and excellent inheritable characters of the selected plant can be kept.

The invention discloses an enterobacter cloacae strain and applications of the enterobacter cloacae strain. The enterobacter cloacae strain is assigned with the accession number of CGMCC No.12819. The invention discloses the enterobacter cloacae which is obtained by separating the soil at the root of tobacco and can efficiently degrade the bactericide, namely, metalaxyl. The experiment proves that enterobacter cloacae can grow in an LB culture medium by taking metalaxyl as the unique carbon source and energy, and can degrade metalaxyl; under the liquid culture condition with added exogenous nutrients, enterobacter cloacae can degrade 90% or above of metalaxyl with the high concentration being 5mg / L mixed in the culture medium within 48h, and has good degrading effect within the wide pH range. With the degrading fungicide produced by adopting the technical scheme provided by the invention, the metalaxyl pesticide residue in the water body and soil and on the surfaces of tobacco leaves can be efficiently degraded, so that the ecological environment is protected and repaired, and the technical gap of degrading the metalaxyl pesticide residue by adopting enterobacter cloacae at present is made up.

A truncated neuraminidase vaccine for preventing influenza features that its active component is a truncated A / PR / 8 / 34 influenza virus VA gene instead of a full-length one and the shortest potector sequence nt 58-nt1365 or nt 1-nt 1299 in its full nucleoside sequent is determined, so finding an effective approach to prepare said vaccine. Its preparing process is also disclosed.

The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.

The invention provides an organoid technology-based individualized lung cancer primary cell culture method. According to the method, high-throughput screening is carried out tumor cell models in vitrothrough organoid models established in tumor tissues of patients, so as to instruct individualized treatment of the patients. Specifically, excised lung cancer fresh tissues are utilized to carry outorganoid culture to establish individualized lung cancer primary cell models and carry out authentication, so that basis is provided for accurate treatment evaluation of lung cancer.

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) detection method of duck hepatitis B virus (DHBV), and a reagent. Nucleic acid sequences in a conservative region most consistent to a DHBV Core region are used as a template to design and synthesize a primer and a fluorescent probe. A pGEM-T / DHBV Core recombinant plasmid is established to prepare a PCR standard product. A reaction system and amplification conditions are optimized. The fluorescence quantitative PCR detection method provided by the invention has the advantages of strong versatility, high sensitivity, strong specificity, good reproducibility and the like, and is suitable for detection of DHBV DNA (deoxyribonucleic acid) of different duck species in different regions.

The present invention discloses a fluorescent quantitative test strip for the porcine epidemic diarrhea virus (PEDV), and a preparation method and application thereof. The fluorescent quantitative test strip comprises a sample pad, a fluorescent label binding pad sprayed with a fluorescent label probe, a nitrocellulose membrane sprayed with a detection line T and a quality control line C, and a piece of absorbent paper that are arranged sequentially in the order of connection. The fluorescent quantitative test strip further comprises a plastic bottom liner arranged in the lower part. The plastic bottom liner is configured to provide an assembly platform; the fluorescent label binding pad is sprayed with a PEDV monoclonal antibody fluorescent microsphere label and a goat-anti-rabbit IgG fluorescent microsphere label; and the nitrocellulose membrane is sprayed with a detection line T formed by the PEDV monoclonal antibody and a quality control line C formed by the goat-anti-rabbit IgG polyclonal antibody. Experiments have proved that by using the test strip provided by the present invention, the sensibility is at least 10 times higher than that by using the existing RT-PCR and colloidal gold test strip detection method, the test sample is initially quantified, and the test result is digitized, so that the human subjective bias is avoided, and a technical means is provided for early diagnosis of the PEDV.

The invention disclosed a way of preserving the big fungus strain for a short term. It comprises the following steps: (1) inoculating the strain to the solid medium and cutting the medium into several pieces when the hyphal body nearly covered the whole flat plate; (2) adding sterile distilled water to the container first, then putting the pieces of medium in the container; covering the lid and preserving it at 4 Deg C. The invention can preserve the strain for a short term without variation; besides, it's very simple and economical.

The invention discloses a breeding seedling-growing method for camellia. The breeding seedling-growing method specifically comprises the steps of selecting seeds, soaking, carrying out disinfection and germination acceleration, putting germination-accelerated seeds into a seedling-growing matrix prepared from pine needle meal, pseudo-ginseng powder, poria powder, coco coir and peat soil through fermentation, covering the seedling-growing matrix with a mixture of plant ash, coco coir and sandy soil, starting seedling growing, and transplanting the seedlings into a planting pot after germination, so as to finish seedling-growing and breeding. The camellia cultured by virtue of the breeding seedling-growing method is high in germination speed, survival rate and ornamental value and can adequately meet the market demands, leaves are glossy, the raw materials of the breeding seedling-growing method are easily available, the cost is low, time and the labor are saved, and the breeding seedling-growing method has considerable economic benefits.

The invention relates to phlebia acerina strain and application thereof in degrading metalaxyl pesticide residue, and belongs to the biological technical field of organisms. The strain is utilized for degrading high-concentration metalaxyl pesticide residue. According to the identification, the scientific name of the white-rot fungi is phlebia acerina Y3667 which is stored in the Common Organism Center of the China Committee for Culture Collection of Microorganisms, wherein the storage number is CGMCC 6809; the ITS (Internal Transcribed Spacers) sequences are extracted by the DNA, cloned and measured; compared with the stored sequences in GenBank, the homology of the phlebia acerina strain with the phlebia acerina strain BMC3014 is 99%. The strain is cultivated in liquid medium to obtain microbial inoculum; and the microbial inoculum is added with the high-concentration metalaxyl pesticide for effectively degrading the metalaxyl pesticide residue. The degrading microbial inoculum generated by the technical scheme in the invention can be used for effectively degreasing the metalaxyl pesticide on the surface of water and agricultural products, so that the ecological environment is protected and restored.

The invention discloses a soil improvement bactericide and a preparation method thereof. Wormcast, humic acid crude powder, potassium hydroxide and microbial cultures are used as main materials, and a wetting dispersant is used as an auxiliary material. The invention belongs to the technology of cycle of organic fertilizers and resources of agricultural wastes. The soil improvement bactericide prepared by adopting the preparation method is high in organic content, strong in biological activity of culture and complete in nutrition, and has the comprehensive effects of increasing roots, strengthening seedling, resisting stress, preventing diseases, resisting lodging, promoting precocity, resisting residue, preventing pollution, increasing yield and improving the soil quality. The soil improvement bactericide is simple in preparation process, low in production cost, easy to operate, agile to produce, convenient to use and easy to popularize and apply in a large area.

The invention discloses a rapid nonsexual systematic mating of Herba Epimedii, which is performed as following: A, forming ridge in greenhouse or plastics greenhouse and paving muck soil with thickness of 10-15cm;B, choosing plants in good health and free from disease, removing overground leaves, taking underground stem part, cutting at the position capable of tillering bud into pieces of 2-3cm with at least on tillering oculus to obtain seedlings; C, soaking in clean water for 1-2h, seeding with spacing in the rows and between the rows of 5cmX5cm-10cmX10cm and sowing depth of 5-8cm, covering soil, irrigating rooting water, and overlaying film; D, ripping away the film after sprouting, watering, weeding, and transplanting after 2-4 months. The invention can maintain properties of the plants. Progenitive first filial generation of Herba Epimedii has stable properties and high survival rate. The invention has simple method and wide application, and is suitable for popularization in countryside.

The invention discloses a method for breeding rice with recessive two-color glume markers. The method includes the following steps that 1, a glume color specific recessive gene donor variety is selected from deep-glume-color rice varieties; 2, after the glume color specific recessive gene donor variety and a rice variety with a Yunnan glume black brown genetic background are hybridized, multi-generation selfing is performed, and a strain with two-color glumes is obtained through breeding; 3, the strain with the two-color glumes is subjected to a multi-point test and a stability test, and a strain which is stable in character, high in resistance and high in adaptability and is provided with the two-color glumes is selected; 4, the strain VII with the two-color glumes and a variety with the normal glume color are hybridized, and the rice with the recessive two-color glume markers is obtained through identification and breeding. According to the method for breeding the rice with the recessive two-color glume markers, a rice recessive special color glume marker gene excavation channel can be expanded.

The invention discloses a preparation method of a yoghurt starter for prolonging the shelf life of yoghurt, which belongs to the field of food preservation. The method comprises the following steps of: performing protoplast fusion on bacillus acidi lactici and lactic acid cocci which are separated out from homemade yoghurt lumps of Xinjiang herders; screening protoplasts after the fusion to obtain target strains; and using the strains as the yoghurt starter to prepare the yoghurt so as to prolong the shelf life of the yoghurt. The yoghurt prepared by using the starter provided by the invention has a long freshness date, and the yoghurt starter is favorable for increasing the yoghurt transport volume and reducing the cost to generate greater economic benefits.

The invention relates to the technical field of tissue culture, in particular to a morinda officinalis tissue culture method. The method comprises the following steps of taking a disinfected and cleaned morinda officinalis rattan as an explant; inoculating the explant to an axillary bud induction culture medium, and illuminating the explant every day until axillary buds grow out; inoculating the axillary buds to a cluster bud induction culture medium, and illuminating the axillary buds every day until cluster buds grow out; and inoculating the cluster buds to a rooting induction culture medium, and illuminating the cluster buds every day until root systems grow out. According to the morinda officinalis tissue culture method, the rattan of the morinda officinalis is adopted as the explant for tissue culture, namely, a direct organogenesis way of the morinda officinalis is used, a callus stage is not needed, good hereditary stability is achieved, variation is not prone to occurring, and the specific medicinal characters of the morinda officinalis can be effectively kept.