100results about How to "Not easy to mutate" patented technology

The invention relates to an embedded antigen receptor based on CD30 and application thereof, and particularly relates to a building method of an embedded antigen receptor T (CAR-T) cell technology based on a tumor specific target site CD30 and application thereof in anti-tumor treatment. The embedded antigen receptor is prepared by connecting an antigen binding structural domain, a transmembrane structural domain, a costimulatory signal conducting region and a CD3 zeta signal conducting structural domain in series, wherein the antigen binding structural domain is combined with tumor-surface antigen, and the tumor-surface antigen is CD30. By adopting the embedded antigen receptor, specific gene transformation on a single chain antibody of the tumor-surface antigen CD30 is carried out, and because of the transformed antibody, the binding force of the antigen-antibody is stronger, and the mutation difficultly occurs; compared with other embedded antigen receptors and other tumor antigens, the embedded antigen receptor has a better effect, and the expression quantity of the target site is high, so that an immunization effect of CAR-T cells is enhanced, and a treatment effect on the CAR-T cells is enhanced.

The invention relates to an embedded antigen receptor based on CD22 and application thereof, and particularly relates to a building method of an embedded antigen receptor T (CAR-T) cell technology based on a tumor specific target site CD22 and application thereof in anti-tumor treatment. The embedded antigen receptor is prepared by connecting an antigen binding structural domain, a transmembrane structural domain, a costimulatory signal conducting region and a CD3 zeta signal conducting structural domain in series, wherein the antigen binding structural domain is combined with tumor-surface antigen, and the tumor-surface antigen is CD22. By adopting the embedded antigen receptor, specific gene transformation on a single chain antibody of the tumor-surface antigen CD22 is carried out, and because of the transformed antibody, the binding force of the antigen-antibody is stronger, and the mutation difficultly occurs; compared with other embedded antigen receptors and other tumor antigens, the embedded antigen receptor has a better effect, and the expression quantity of the target site is high, so that an immunization effect of CAR-T cells is enhanced, and a treatment effect on the CAR-T cells is enhanced.

The invention discloses acinetobacter baumannii, and a screening method and an application thereof in degradation of an azo dye Congo red. The stain is named as acinetobacter baumannii YNWH226, is preserved in China general microbiological culture collection center, and has the preservation number of CGMCC No.7922. The invention provides the new acinetobacter baumannii and the application thereof in degradation of the dye. Especially, the acinetobacter baumannii can effectively degrade the azo dye Congo red, has quite good decoloration and mineralization effects on Congo red wastewater, can completely mineralize the Congo red under a simple aerobic condition, and has great significance on governance of dye pollution and study on aerobic degradation of the azo dye.

The invention relates to a chimeric antigen receptor, and application thereof, specifically to a construction method for a cell technology of a chimeric antigen receptor T (CAR-T) based on a tumor-specific target CD20 and application of the CAR-T to anti-cancer treatment. The chimeric antigen receptor is formed by an antigen binding domain, a transmembrane domain, a costimulatory-signal transduction domain and a CD3 zeta signal transduction structural domain through series connection, wherein the antigen binding domain binds to a tumor surface antigen, and the tumor surface antigen is CD20. The chimeric antigen receptor carries out specific gene modification on a single-chain antibody targeting to the tumor surface antigen CD20, so the modified antibody allows antigen-antibody binding force to be stronger and mutation is hard to occur; and compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor provided by the invention has better effect, a high target expression level and improves the immunization effect and treatment effect of CAR-T cells.

The invention discloses an acid-producing klebsiella oxytoca MOW-02-05, a selection method and an application of the acid-producing Klebsiella oxytoca MOW-02-05, wherein the acid-producing klebsiella oxytoca MOW-02-05 is isolated from an intermittent anaerobic sludge reactor and can efficiently degrade dyes and organisms to produce hydrogen; and the klebsiella oxytoca MOW-02-05 is used for decolorizing azo dyes and biologically produce the hydrogen from organic carbon resources. The strain is identified as klebsiella oxytoca MOW-02-05 and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5237. The strain obtained by the invention can solve a problem caused by acidifying the reactor when a conventional anaerobic reactor treats azo dye wastewater, and is suitable for biological treatment of printing and dyeing dye wastewater.

The invention relates to a chimeric antigen receptor based on CD10, and applications thereof, and more specifically relates to a cell technology construction method of chimeric antigen receptor T (CAR-T) taking tumor specific target spot CD10 as a base, and applications of the chimeric antigen receptor T in treatment of tumor. The chimeric antigen receptor T is composed of an antigen binding domain, a transmembrane domain, a costimulatory signal transduction region, and a CD3 zeta signal transduction domine via series connection; wherein the antigen binding domain is used for binding tumor surface antigens, and the tumor surface antigen is CD10. According to applications, specific gene modification of single-chain antibody of tumor surface antigen CD10 is carried out, the modified antibody is capable of increasing antigen-antibody binding force, mutation is not easily caused. Compared with other chimeric antigen receptors and other tumor antigens, the chimeric antigen receptor possesses following advantages: the effect is better, target spot expression quantity is higher, immune effect on CAR-T cells is improved, and treatment effect of CAR-T cells is improved.

The invention relates to a proliferation accelerant and application thereof, in particular to a fibroblast proliferation accelerant capable of promoting fibroblast proliferation and fibroblast extractand fibroblast extract freeze-dried powder prepared through fibroblasts. The proliferation accelerant comprises loofah saponin and/or momordica saponin. A loofah saponin and momordica saponin combined inducer is adopted for culture of skin fibroblasts, so that synthesis and secretion of bioactive substances derived from the skin fibroblasts are promoted effectively, content of active ingredientsin cell derivatives is increased, and production efficiency and biologic effect of the skin fibroblast derived cell derivatives.

A truncated neuraminidase vaccine for preventing influenza features that its active component is a truncated A/PR/8/34 influenza virus VA gene instead of a full-length one and the shortest potector sequence nt 58-nt1365 or nt 1-nt 1299 in its full nucleoside sequent is determined, so finding an effective approach to prepare said vaccine. Its preparing process is also disclosed.

The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) detection method of duck hepatitis B virus (DHBV), and a reagent. Nucleic acid sequences in a conservative region most consistent to a DHBV Core region are used as a template to design and synthesize a primer and a fluorescent probe. A pGEM-T/DHBV Core recombinant plasmid is established to prepare a PCR standard product. A reaction system and amplification conditions are optimized. The fluorescence quantitative PCR detection method provided by the invention has the advantages of strong versatility, high sensitivity, strong specificity, good reproducibility and the like, and is suitable for detection of DHBV DNA (deoxyribonucleic acid) of different duck species in different regions.

The invention discloses a breeding seedling-growing method for camellia. The breeding seedling-growing method specifically comprises the steps of selecting seeds, soaking, carrying out disinfection and germination acceleration, putting germination-accelerated seeds into a seedling-growing matrix prepared from pine needle meal, pseudo-ginseng powder, poria powder, coco coir and peat soil through fermentation, covering the seedling-growing matrix with a mixture of plant ash, coco coir and sandy soil, starting seedling growing, and transplanting the seedlings into a planting pot after germination, so as to finish seedling-growing and breeding. The camellia cultured by virtue of the breeding seedling-growing method is high in germination speed, survival rate and ornamental value and can adequately meet the market demands, leaves are glossy, the raw materials of the breeding seedling-growing method are easily available, the cost is low, time and the labor are saved, and the breeding seedling-growing method has considerable economic benefits.

The invention discloses a method for breeding rice with recessive two-color glume markers. The method includes the following steps that 1, a glume color specific recessive gene donor variety is selected from deep-glume-color rice varieties; 2, after the glume color specific recessive gene donor variety and a rice variety with a Yunnan glume black brown genetic background are hybridized, multi-generation selfing is performed, and a strain with two-color glumes is obtained through breeding; 3, the strain with the two-color glumes is subjected to a multi-point test and a stability test, and a strain which is stable in character, high in resistance and high in adaptability and is provided with the two-color glumes is selected; 4, the strain VII with the two-color glumes and a variety with the normal glume color are hybridized, and the rice with the recessive two-color glume markers is obtained through identification and breeding. According to the method for breeding the rice with the recessive two-color glume markers, a rice recessive special color glume marker gene excavation channel can be expanded.

The invention discloses a preparation method of a yoghurt starter for prolonging the shelf life of yoghurt, which belongs to the field of food preservation. The method comprises the following steps of: performing protoplast fusion on bacillus acidi lactici and lactic acid cocci which are separated out from homemade yoghurt lumps of Xinjiang herders; screening protoplasts after the fusion to obtain target strains; and using the strains as the yoghurt starter to prepare the yoghurt so as to prolong the shelf life of the yoghurt. The yoghurt prepared by using the starter provided by the invention has a long freshness date, and the yoghurt starter is favorable for increasing the yoghurt transport volume and reducing the cost to generate greater economic benefits.