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Tissue culture rapid propagation method of eucalyptus cloeziana

A large-inflorescence Eucalyptus, tissue culture fast propagation technology, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc. And the effect of reliable resistance to stress, low production cost, and low difficulty in operation

Active Publication Date: 2016-04-06
广西壮族自治区国有东门林场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most importantly, there has been no report on the establishment of tissue-cultured clones of Eucalyptus grandis

Method used

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  • Tissue culture rapid propagation method of eucalyptus cloeziana
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  • Tissue culture rapid propagation method of eucalyptus cloeziana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for tissue culture and rapid propagation of Eucalyptus grandis, the steps of which are as follows:

[0052] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus grandis suitable for local growth;

[0053] (2) In the excellent family of Eucalyptus grandis, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0054] (3) Cut down the fine individual plants at a height of 15cm from the ground to promote germination. When the sprouting strips grow to 12cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0055] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 3 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0056] (5) ...

Embodiment 2

[0066] A method for tissue culture and rapid propagation of Eucalyptus grandis, the steps of which are as follows:

[0067] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus grandis suitable for local growth;

[0068] (2) In the excellent family of Eucalyptus grandis, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0069] (3) Cut down the fine individual plants at a height of 18cm from the ground to promote germination. When the sprouting strips grow to 15cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0070] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 4 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0071] (5) ...

Embodiment 3

[0081] A method for tissue culture and rapid propagation of Eucalyptus grandis, the steps of which are as follows:

[0082] (1) In the family test forest introduced in Australia, after testing and comparison, select an excellent family of Eucalyptus grandis suitable for local growth;

[0083] (2) In the excellent family of Eucalyptus grandis, select excellent individual plants after comprehensive comparison by measuring diameter at breast height, tree height, stock volume, annual growth, wood properties and dry shape indicators;

[0084] (3) Cut down the fine individual plants at a height of 20cm from the ground to promote germination. When the sprouting strips grow to 18cm, cut the sprouting strips, soak the base in water and bring them back to the laboratory for later use;

[0085] (4) Trim the bud strips to remove leaves and petioles, rinse with running water for 5 minutes, then wash with saturated detergent solution for 10 minutes, and clean with pure water;

[0086] (5) ...

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Abstract

The invention discloses a tissue culture rapid propagation method of eucalyptus cloeziana. The method adopts an excellent single plant of an excellent eucalyptus cloeziana family, which is cut down and promoted to germinate, a bud serves as an explant, a stem section with a resting bud induces a sterile bud in vitro, the sterile bud is obtained without a callus seedling path, the stem section of the explant directly grows a side bud or an adventitious bud, and the sterile bud is subjected to subculture enrichment culture, rooting culture and rooting seedling transplanting to obtain a regenerated plant. The tissue culture rapid propagation method of eucalyptus cloeziana is lower in operation difficulty, stable in the growth of a sterile bud propagation system and good in repeatability and can be used for scale expanding propagation repeatedly, after the rooting culture of cut buds, the rooting rate is higher, the popularization value is realized, rooting seedlings can survive after transplanting, seedlings normally grow, the obtained regenerated plant can robustly grow in a large field, a survival rate of afforestation is more than 95% and excellent inheritable characters of the selected plant can be kept.

Description

technical field [0001] The invention relates to the technical field of eucalyptus cultivation, in particular to a method for tissue culture and rapid propagation of Eucalyptus grandis. Background technique [0002] Eucalyptus is native to Australia and is a collective name for three genera of trees in the family Mytaceae, Eucalyptus, Angophora, and Corymbia. A total of 1039 species, subspecies and varieties, Eucalyptus grandis belongs to a species of Eucalyptus. Because it is naturally distributed in Queensland, Australia, it is also known as Queensland Eucalyptus. [0003] Eucalyptus has become one of the three world-recognized plantation tree species due to its fast growth, wide range of uses and high economic benefits. The introduction, cultivation and development of eucalyptus in my country is very rapid, and it has played a prominent role in the development of China's forestry. By 2013, the area of ​​China's eucalyptus plantations reached 4.4 million hectares, accoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008Y02A40/22Y02P60/40
Inventor 唐再生苏勇莫继有李炳寿李丽芳石前熊涛王建忠张磊黎怀玲兰俊陈东林梁秀莉刘鑫邓冬丽
Owner 广西壮族自治区国有东门林场
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