The invention discloses an enzymolysis preparation method of 
Cannabis sativa seed 
meal polypeptide with alpha-
glucosidase inhibiting activity. The method includes: 
drying Cannabis sativa seed 
meal and crushing it into 
powder, adding water and stirring the mixture evenly, adjusting the pH to 8.0-10.5, adjusting the temperature to 30-80DEG C, performing stirring at a uniform speed for 0.5h-2h, conducting 
centrifugation and adjusting the pH of the supernatant to 3-6, thus obtaining 
protein precipitate; adding water to dissolve the 
protein, with the proportion being 2-7g:100mL, adjusting the pH to 7.0- 9.5, then adding 
alkaline protease, with the ratio of the 
protein weight to the 
enzyme activity being 0.1-5:1000, keeping the temperature at 50-75DEG C, and controlling the time at 1-4h; subjecting the enzymatic 
hydrolysate to separation and purification through macroporous adsorption resin and a 
Sephadex column, and carrying out collecting and 
drying, thus obtaining the alpha-
glucosidase inhibiting active 
peptide powder. The 
Cannabis sativa seed 
meal polypeptide provided by the invention adds new sources for alpha-
glucosidase inhibiting active substances, realizes full utilization of raw materials, and has the advantages of high product activity, convenient and feasible preparation process, and 
environmental protection, etc.