The invention relates to a multi-target picture segmentation based on a level set, and the multi-target picture segmentation based on the level set is applied in the technical field of the image analysis. The method includes the following steps: firstly, drawing one closed curve or more than one closed curves on to-be-segmented images as an initial outline; secondly, utilizing an initiative outline model based on a region, carrying out an iteration revolution on the initiative outline, and obtaining a profile curve of a target. The initiative outline model based on the region fully takes partial gray level information of the image into account. Therefore, the multi-target picture segmentation based on the level set is capable of segmenting images with uneven gray levels, extending the model into multiple orders, and segmenting over the multi-target images. Compared with the prior art, the multi-target picture segmentation based on the level set has the advantages of being insensitive in initialization, quick in calculation speed, strong in anti-noise ability and accurate in segmentation results. Meanwhile, the multi-target picture segmentation based on the level set is capable of segmenting medical images provided with a plurality of different targets.