The invention discloses an enamel
secretion prolonging and thickness increasing method based on KLK4
alpha protein, and relates to the technical field of
biomedicine and dental materials.According to the method, expression of the KLK4
alpha protein is specifically regulated and controlled, forward intervention in the
secretion period in the enamel development process is achieved, synthesis and accumulation of enamel matrix
protein are directly promoted, and the effect of prolonging the
secretion period of the enamel is achieved. A recombinant lentiviral vector mediated
gene transduction technology is utilized to specifically introduce the KLK4
alpha gene into a dental
embryo glazing cell and realize overexpression, and the expressed KLK4
alpha protein remarkably delays the process of transformation from a secretion period to a mature period by regulating the secretion activity of the
glazing cell, so that the expression level of the KLK4alpha
protein is improved, and the expression level of the KLK4alpha
protein is improved. The
time sequence intervention effectively prolongs the secretion
window period of key
organic matrix components such as
glaze protein,
glaze protein and the like, so that before mineralization is started, the total amount of
extracellular matrix net racks is substantially increased, a space is provided for
nucleation of hydroxyapatite crystals, and finally, the thickness of a mineral phase is remarkably increased; and the protection performance of the dental crown structure is enhanced.