The invention provides a method capable of rapidly selecting and screening surfactant
petroleum hydrocarbon degradation pure
bacterial strain from oil soil. According to the method capable of rapidly selecting and screening the surfactant
hydrocarbon degradation
bacteria on the surface of forming material, the oil soil is collected from an
oil field and added into a
hydrocarbon degradation culture medium with the amount of 5%, wherein
crude oil of the
hydrocarbon degradation culture medium is a sole
carbon source, the content of the
crude oil is gradually improved, and an
enrichment culture is carried out on a constant temperature
shaker. After the enriched
bacteria liquid is diluted to a certain degree and coated to be cultured on a blood plate, 1-3d is cultured at the temperature of 30 DEG C,
bacteria colony with a clear and large transparent zone edge on the blood plate is taken, after the bacteria colony is cultured in a liquid meat peptone culture medium, the bacteria liquid is dipped through a sterilizing
toothpick blunt end and dibbled on an oil plate, 3d is cultured at the temperature of 30 DEG C, bacteria colony with an obvious and large oil
biting spot is taken to be vaccinated in a meat peptone inclined surface and stored, after the bacteria colony is cultured with the meat peptone culture medium, the
hydrocarbon degradation culture medium and the surfactant culture medium are vaccinated in sequence, 7d and 3d are cultured at the temperature of 30 DEG C, the
hydrocarbon degradation capacity and the surfactant capacity are determined, and therefore the surfactant
petroleum hydrocarbon degradation
bacterial strain can be obtained.