The invention discloses composition for promoting regeneration of dental pulp and dentin. The composition comprises a PDGF (platelet-derived growth factor) and a scaffold, wherein the PDGF adopts one or more of PDGF-BB, PDGF-AB and PDGF-AA; the scaffold adopts one or more of alginate, Arabic gum, guar gum, xanthan gum, gelatin, chitin, chitosan, chitosan acetate, chitosan lactate, chondroitin sulfate, N,O-carboxymethyl chitosan, glucan, fibrin glue, fibrinogen, natural blood clots, glycerol, hyaluronic acid, gourmet powder, glucosamine, proteoglycan, starch, lactic acid, poloxamer, sodium glycero-phosphate, collagen, glycogen, a keratin filament, a synthetic surgical sealant and a synthetic surgical adhesive. The composition can be conveniently injected into a dental pulp cavity required to be treated, repairing cells enter the dental pulp cavity and play the repair role under combined action of the PDGF and the scaffold, so that the diseased dental pulp and dentin are regenerated and repaired.