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Tissue engineering dental pulp based on digital optical processing printing technology and preparation method

A technology of digital optics and tissue engineering, applied in biochemical equipment and methods, tissue regeneration, tissue culture, etc., can solve the problems of increased fragility and easy staining of dental hard tissues, and achieve the effect of promoting dental pulp regeneration

Pending Publication Date: 2021-11-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, root canal therapy is the most routine clinical treatment for irreversible pulp lesions or periapical lesions. However, dental hard tissues that have lost dental pulp have problems such as increased fragility and easy staining. Pulpectomy, Pulp revascularization, root apex induction and other methods cannot perfectly solve this problem, and it is urgent to find a new method

Method used

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  • Tissue engineering dental pulp based on digital optical processing printing technology and preparation method
  • Tissue engineering dental pulp based on digital optical processing printing technology and preparation method
  • Tissue engineering dental pulp based on digital optical processing printing technology and preparation method

Examples

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preparation example Construction

[0033] A method for preparing tissue-engineered dental pulp based on digital optical processing and printing technology, the preparation method comprising the following steps:

[0034] The acquisition and cultivation of dental pulp mesenchymal stem cells;

[0035] Preparation of GelMA hydrogel;

[0036] After the dental pulp mesenchymal stem cells obtained in the above steps were digested and centrifuged, they were resuspended with medium, and the resulting cell suspension was mixed with GelMA (1M) hydrogel evenly, and then the bioink was transferred to the sample tank of the 3D printer (already Preheat to 37°C), and print layer by layer through digital light processing 3D printing technology.

[0037] According to the above preparation method, wherein the cell suspension is a cell suspension containing dental pulp mesenchymal stem cells.

[0038] The above-mentioned application of tissue engineering dental pulp based on digital optical processing and printing technology.

...

Embodiment 1

[0057] Example 1: Preparation of tissue engineered dental pulp based on digital optical processing and printing technology

[0058] (1) Cultivation and expansion of dental pulp cells: the patient’s teeth were quickly stored in α-MEM medium (Gibco, USA) containing penicillin (100 Units / mL) and streptomycin (100 Units / mL), and transferred into ultra-clean Taiwan. Disinfect the water and air pipes of the turbine with povidone iodine. After cross-sectioning the tooth, use a probe to take out the pulp completely, wash it three times with PBS containing penicillin (100Units / mL) and streptomycin (100Units / mL), and transfer it to the EP tube. Cut the pulp tissue into pieces with tissue scissors to a size of about 1mm*1mm*1mm, digest the pulp tissue with a final concentration of 3mg / mL of Collagenase and 4mg / mL of Dispase, and act on a shaker at 37°C for 30 minute. After centrifugation at 1200rpm for 3 minutes at room temperature, discard the supernatant, transfer the digested tissue...

Embodiment 2

[0065] Example 2: Application of tissue engineered dental pulp based on digital optical processing and printing technology in nude mouse ectopic dental pulp regeneration model

[0066]Select the GelMA hydrogel microspheres loaded with dental pulp mesenchymal stem cells prepared in Example 1, and use a 20G (inner diameter of 620 μm, flat head) injection needle to inject the 3D-printed micropolymers with cells into the root canal of the dentin fragment, The root canal opening was covered with gelatin sponge (Microsphere group), and the prepared graft complexes were placed horizontally in a twelve-well plate, and ordinary complete medium (Gibco, USA) (containing double antibodies) was added at 37°C, 5% CO 2 After incubating in the environment for one day (standstill), the graft complexes of the printed microcarrier (Microsphere) group were buried subcutaneously in a nude mouse under general anesthesia. After the nude mice were fed for 8 weeks, the transplanted complex was taken o...

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Abstract

The invention discloses tissue engineering dental pulp based on a digital optical processing printing technology. The tissue engineering dental pulp is composed of hydrogel microspheres loaded with dental pulp mesenchymal stem cells. A 3D printing technology based on digital optical processing is adopted, high-precision dental pulp mesenchymal stem cell carrying microspheres can be rapidly produced, and the activity function of cells is guaranteed on the basis that the form and speed are guaranteed. A microsphere implant with the micron-sized diameter is firstly beneficial to injection treatment, the anatomical form of a root canal is slender, the root tip cannot be completely filled with a preformed tissue structure by using a filling instrument, and the injectable microsphere can be brought to the vicinity of the root tip hole; and secondly, more gaps can be generated among different microspheres, so that a dental pulp cavity can be refilled with blood, and necessary nutrients are provided for regeneration. The proper microsphere size provides convenience for internal cells to ingest nutrient substances, facilitates nutrient substance exchange and waste metabolism of cells and blood, provides a proper microenvironment for cell growth, and promotes dental pulp regeneration to the maximum extent.

Description

technical field [0001] The invention belongs to the technical field of oral medical devices, in particular to a tissue-engineered dental pulp based on digital optical processing and printing technology and a preparation method thereof. Background technique [0002] Dental pulp develops from the ectodermal mesenchyme, and contains nerves, blood vessels, dental pulp mesenchymal stem cells, odontoblasts and other components, which mainly play the roles of sensation, nutrition, dentin formation, defense and repair[1]. Due to the synergistic effect of factors such as the existence of hard dentin with no tolerance around the pulp, small compound apical foramen, and no effective collateral circulation, it is difficult to recover once the pulp is inflamed, subjected to other stimuli, and damaged[2, 3]. At present, root canal therapy is the most routine clinical treatment for irreversible pulp lesions or periapical lesions. However, dental hard tissues that have lost dental pulp hav...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61L27/22A61L27/38A61L27/50
CPCC12N5/0664A61L27/3834A61L27/3865A61L27/222A61L27/50C12N2533/54C12N2533/30A61L2430/12A61L2400/06A61L2300/412
Inventor 俞梦飞龚佳幸陆科杰钱颖朱子羽张超颖周思怡王慧明
Owner ZHEJIANG UNIV
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