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Macromolecular microcarrier and preparation method thereof

A silk fibroin and microcarrier technology, applied in the field of biomedical materials, can solve problems such as unreported, and achieve the effects of simple equipment, increased specific surface area, high drug loading rate and embedding rate of microspheres

Inactive Publication Date: 2011-02-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology of preparing silk fibroin into cell microcarriers has not been reported so far.

Method used

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  • Macromolecular microcarrier and preparation method thereof
  • Macromolecular microcarrier and preparation method thereof
  • Macromolecular microcarrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Put 150 g of silkworm silk into 6 L of 0.05% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 30 minutes, repeated 3 times to degumming silk, fully washed and dried to obtain pure silk fiber. Add pure silk fiber to the ternary solution (by CaCl 2 / water / ethanol molar ratio is 1:8:2) solution, at 72 o C stirring and dissolving into a mixed solution of silk fibroin. Put the obtained silk fibroin mixed solution into a dialysis bag, and dialyze with deionized water for 4 days to remove impurities (Li, Br, etc.) to obtain a pure silk fibroin solution.

[0032] Adjust the mass concentration of the silk fibroin solution to 3%, and evenly add 20% EDC, 10% NHS, and 20% MES into the silk fibroin solution to obtain a silk shell solution.

[0033] The vascular endothelial growth factor was configured as a water-soluble drug core layer solution with a concentration of 800ng / ml.

[0034] Add the silk fibroin shell solution and the water-soluble drug core layer solutio...

Embodiment 2

[0040] Put 100 g of tussah raw silk into 5 L of 0.25% Na 2 CO 3 In aqueous solution, at 98~100 o C treatment for 45 min, repeated 3 times to degumming silk, fully washed and dried to obtain tussah silk fiber. Add tussah silk fiber into molten calcium nitrate tetrahydrate, at 105 o C stirring and dissolving into tussah silk protein mixed solution. The obtained mixed solution was put into a dialysis bag, and dialyzed with deionized water for 4 days to remove impurities to obtain a pure tussah silk fibroin protein solution.

[0041] Adjust the mass concentration of tussah silk fibroin solution to 1.5%, and add 20% EDC, 10% NHS, and 20% MES into the silk fibroin solution evenly to obtain a silk shell solution. The insulin-like growth factor was configured as a water-soluble drug core layer solution with a concentration of 500ng / ml.

[0042] The two solutions of the shell layer and the core layer were poured into the syringe respectively, the fixed voltage was 6 kV, the pole d...

Embodiment 3

[0046] Use boiling Na with a concentration of 3.5‰ 2 CO 3 The silkworm cocoons were treated with solution three times, each time for 30 min, to degumming the silk, and after washing and drying thoroughly, the silk fibroin fiber was obtained. Add celestial silk fibroin fiber into molten calcium nitrate tetrahydrate, at 90 o C stirring and dissolving into a mixed solution of silk fibroin protein. The obtained mixed solution was put into a dialysis bag, and dialyzed with deionized water for 4 days to remove impurities to obtain a pure silk fibroin solution.

[0047] Adjust the concentration of the silk fibroin solution to 2%, and evenly add 20% EDC, 10% NHS, and 20% MES of the mass of silk fibroin into the silk fibroin solution to obtain a shell solution.

[0048] The nerve growth factor was configured as a water-soluble drug core layer solution with a concentration of 200ng / ml.

[0049] The two solutions of the shell layer and the core layer were poured into the syringe resp...

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Abstract

The invention discloses a macromolecular microcarrier and a preparation method thereof, belonging to the technical field of biomedicine. The preparation method comprises the following steps of: splitting a silk solution and a drug solution into micrometer grade liquid drops with core-shell structures under the action of a high-voltage electric field by adopting a coaxial high-voltage electrostatic technology and a freeze drying method; concreting through liquid nitrogen, and then preparing the microcarrier which is difficult to dissolve in water through freeze drying. The microcarrier is in the shape of a microsphere with the core-shell structure, and the diameter of the microsphere is 100-500 micrometers; the shell of the microcarrier comprises the components of silk fibroin; the random-coil conformation of silk fibroin molecules is 80-90 percent; the shell is in a porous structure, and an aperture is 5-20 micrometers; and a core of the microcarrier comprises the components of water-soluble drugs. The silk microcarrier has extensive application prospect in the fields of cell culture, drug release, and the like.

Description

technical field [0001] The invention relates to a polymer microcarrier and a preparation method thereof, in particular to a silk fibroin microcarrier using silk fibroin as a main raw material and carrying water-soluble drugs and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Polymer microspheres have special functions that other materials do not have, and are one of the current research hotspots. Microspheres have a wide range of uses and can be used for drug carriers, immobilization of enzymes and cells, separation and purification of biologically active substances, and cell culture carriers. Among them, research on using microspheres as cell growth and proliferation carriers (microcarriers) has gradually attracted people's attention. focus on. Microcarriers refer to microbeads with a diameter of 60-250 μm, which are used for anchorage-dependent cell growth. Their huge specific surface area can increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K9/16A61K38/18A61P43/00A61K38/30
Inventor 王璐李明忠吕强卢神州
Owner SUZHOU UNIV
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