11 results about "Random coil" patented technology

The present disclosure relates to uricase variant polypeptides and their multimers, as well as uricase conjugates comprising (i) a first domain comprising one of the uricase variant polypeptides conjugated to (ii) a second domain comprising a random coil polypeptide domain. Methods of using the uricase variant polypeptides and uricase conjugates to treat gout, tumor lysis syndrome, and other diseases associated with elevated uric acid levels in body fluids, including blood, are provided. Methods of recombinantly producing the uricase variant polypeptides and uricase conjugates disclosed herein are also provided.

The invention discloses a water-soluble silk fibroin with a random crimp / Silk I structure as a main structure as well as a preparation method and application of the water-soluble silk fibroin. The silk fibroin product is characterized in that in a protein secondary structure, the total content of random curls and Silk I structures exceeds 85%, and the proportion of Silk I in the whole crystal structure is not lower than 75%; the product can be directly dissolved in water at room temperature to form a stable clarified solution with the concentration not lower than 5%, and is not gelatinized after being stored at 4 DEG C for 90 days. The preparation method comprises the following steps: degumming and cleaning the mulberry silk raw material; the method comprises the following steps: carrying out short-time (less than or equal to 20 minutes) high-temperature dissolution by adopting a multi-element solution dissolution system with a specific ratio, and then carrying out dialysis purification at a low temperature by taking the conductivity (lt, 10 [mu] S / cm) of a dialysis external liquid and the concentration (lt, 50 ppm) of calcium ions as double end-point standards. The directional regulation and control of the silk fibroin structure are realized through the accurate control of the whole process, and the obtained product has excellent water solubility, storage stability and biological safety, and can be widely applied to the fields of drug delivery, 3D biological printing, medical beauty filling, tissue engineering and the like.

The invention belongs to the technical field of food-borne functional factors, and particularly relates to a bioactive glycopeptide as well as a preparation method and application thereof. According to the application, casein glycomacropeptide is taken as a substrate, specific protease is adopted for carrying out directional enzymolysis reaction on the casein glycomacropeptide, and after primary separation and purification by hydrophilic interaction chromatography and secondary purification by cation exchange chromatography are carried out, the bioactive glycopeptide with the molecular weight of less than 1,500 Da and more than 80% of components and the random coil secondary structure content of more than 35% is obtained. A system performance test shows that in an in-vitro cell experiment, the bioactive glycopeptide prepared by the embodiment of the invention can remarkably improve the proliferation rate of preosteoblasts and lay a sufficient cell quantity foundation for bone cell generation; meanwhile, the activity of an osteogenic early differentiation key marker ALP is effectively up-regulated, and the differentiation process of preosteoblasts towards the osteoblast direction is accelerated; in addition, the expression quantity of osteogenesis-related regulatory protein calcium-sensitive receptors is remarkably increased, and the function improvement of cells to mature bone cells is further promoted.

The application discloses an engineered nitrile hydratase and application thereof. The engineered nitrile hydratase is based on a wild-type nitrile hydratase and comprises a random coil domain located near an active center and having a sequence of SEQ ID NO. 1, wherein methionine in the random coil domain is replaced by any one of glycine, leucine, isoleucine, glutamine, threonine, glutamic acid, arginine and lysine. The engineered nitrile hydratase with any one of the above mutations has higher enzyme activity and improves catalytic efficiency.

The invention discloses a dry and wet dual-purpose biological adhesive and a preparation method thereof.The preparation method comprises the steps that a polypeptide aqueous solution and a polyethylene glycol dimaleimide aqueous solution are mixed, the pH is adjusted to 7-8, and R-liquid is obtained; and mixing the R-liquid with a chelating agent, and drying to obtain the dry and wet dual-purpose biological adhesive. The designed Switch polypeptide can be complexed with Zn ions, after the Zn ions are introduced into R-liquid, the Switch polypeptide is subjected to conformation transformation from a random coil mode to a spiral coil coiling mode, and the change caused by the conformation transformation is mainly reflected in that hydrophilic and hydrophobic amino acids of the polypeptide can be spatially rearranged according to the spiral coil coiling rule; hydrophilic amino acids are completely exposed on the outer side, hydrophobic amino acids are orderly arranged on the inner side, hydrophobic side chains are also completely stretched, the hydrophobic amino acid side chains complete high-strength adhesion of polypeptide and an interface, and the biological adhesive achieves the adhesion effect according to the principle.

This invention provides an innovative means and method for coupling dialkylamines and polypeptides or peptides capable of forming random coil conformations. This invention also provides innovative compounds prepared by the methods detailed herein, wherein the compounds are characterized by formula (I): A-[B-] b C-D. The compounds comprise polypeptides / peptides capable of forming a random coil conformation, an N-terminal protecting group, a dialkylamino group, and optionally a linker. Furthermore, the present invention provides lipid nanoparticles comprising the compounds characterized in formula (I), and means and methods for preparing the lipid nanoparticles. Formulations comprising the compounds of the present invention and / or lipid nanoparticles are also provided. Furthermore, the present invention relates to the use of the innovative compounds, lipid nanoparticles, and / or formulations disclosed herein.

The present invention provides a novel silk fibroin crosslinking agent, a method for preparing the same, and use thereof. In the silk fibroin crosslinking agent, the silk fibroin has a structure that can easily form crosslinked microcrystals, and includes silk fibroin having less than 40% of random coil structure. The present invention also provides a gel formed using the crosslinking agent and a method for preparing the same.

The application discloses a dry-wet type biological adhesive and a preparation method thereof, and the preparation method comprises the following steps: mixing a polypeptide aqueous solution and a polyethylene glycol dimaleimide aqueous solution, adjusting the pH to 7-8 to obtain R-liquid; and mixing the R-liquid with a chelating agent and drying to obtain the dry-wet type biological adhesive. The Switcch polypeptide designed in the application can complex Zn ions, and after the introduction of Zn ions into the R-liquid, the Switcch polypeptide performs a conformational transition from a random coil mode to a helical coil mode, and the change brought by the conformational transition is mainly reflected in that the hydrophilic and hydrophobic amino acids of the polypeptide are rearranged in space according to the rules of the helical coil winding; the hydrophilic amino acids are completely exposed on the outside, the hydrophobic amino acids are orderly arranged on the inside, the hydrophobic side chains are completely stretched, the hydrophobic amino acid side chains complete the high-strength adhesion of the polypeptide and the interface, and the biological adhesive realizes the adhesion effect according to the principle.

The present invention provides novel uricase conjugates comprising a first domain and a second domain. The first domain comprises a uricase polypeptide and the second domain is an unstructured, random coil polypeptide domain comprising at least about 100 amino acids. Methods for using the uricase conjugates of the invention, for example, in the treatment of tumor lysis syndrome and / or gout, are also provided.

This invention relates to a transparent Bordeaux mixture nanosuspension and its preparation method, wherein the basic copper sulfate particles in the suspension are smaller than 100 nanometers. The basic copper sulfate particles in the nanosuspension are suspended and dispersed in water in a random coil morphology using a water-soluble polymeric dispersant.

The application discloses a silk fibroin-based piezoelectric sensing film induced by BTO conformation transition and a preparation method thereof. The sensing film is composed of regenerated silk fibroin (SF) and tetragonal barium titanate (BaTiO3) nanoparticles by electrospinning technology. The core of the application is that the polar surface charge of BaTiO3 nanoparticles is used to in-situ induce the silk fibroin molecular chain to transform from random coil to beta sheet crystal phase with piezoelectric activity under the synergistic effect of high-voltage electric field in electrospinning, and a double piezoelectric synergistic system of organic crystal phase and inorganic ceramic phase is constructed. Experiments prove that the sensing film has excellent flexibility, biocompatibility and extremely high low-frequency sensitivity (0.25Hz-1Hz), and is stable under 10000 times of cyclic loading. The application has important application value in the fields of flexible self-powered physiological sensing such as heart sound monitoring and pulse recognition.