60 results about "Random coil" patented technology

Percutaneous interbody spine fusion devices are provided. These devices may have a number of different designs and exemplary features. One device consists of a single rotating hollow cam cage with perforations (with or without fixation anchors) and a delivery tool. Another device consists of a counter-rotating cam cage (with or without fixation anchors) and a delivery tool. A third device consists of an expanding cam with anchors and delivery tool; this device may consist of a single expanding cam or a series of expanding cams. A delivery tool is included. A fourth device consists of a spring cage; this device may be a stand-alone device, can be combined with expanding cam device, and may be incorporated into a cage. A delivery tool is included. This spring cage may or may not have fixation anchors. A fifth device consists of a random coil support device that can be used as a nuclear or spine fracture support device; a delivery tool is included. A sixth device consists of a directional ribbon strip coil device and delivery tool. Also provided is a percutaneous off-angle bone stapling / nailing fixation device.

The present invention relates to a display comprising at least one substrate, at least one electrically conductive layer and at least one electronically modulated imaging layer, wherein the electronically modulated imaging layer comprises a self-assembled, close-packed, ordered monolayer of domains of electrically modulated material in a random coil fixed polymer matrix and filler.

The invention discloses a macromolecular microcarrier and a preparation method thereof, belonging to the technical field of biomedicine. The preparation method comprises the following steps of: splitting a silk solution and a drug solution into micrometer grade liquid drops with core-shell structures under the action of a high-voltage electric field by adopting a coaxial high-voltage electrostatic technology and a freeze drying method; concreting through liquid nitrogen, and then preparing the microcarrier which is difficult to dissolve in water through freeze drying. The microcarrier is in the shape of a microsphere with the core-shell structure, and the diameter of the microsphere is 100-500 micrometers; the shell of the microcarrier comprises the components of silk fibroin; the random-coil conformation of silk fibroin molecules is 80-90 percent; the shell is in a porous structure, and an aperture is 5-20 micrometers; and a core of the microcarrier comprises the components of water-soluble drugs. The silk microcarrier has extensive application prospect in the fields of cell culture, drug release, and the like.

The invention discloses silk fibroin hydrogel adopting an IPN (interpenetrating polymer network) structure and a preparation method of the silk fibroin hydrogel. N-vinylpyrrolidone monomers are subjected to a polymerization reaction under catalysis of horse radish peroxidase to produce polyvinylpyrrolidone, polyvinylpyrrolidone and silk fibroin macromolecules are subjected to molecular chain entanglement, and the IPN hydrogel with a random coil structure as a main structure is formed. According to the solid content of the hydrogel, the content of silk fibroin is 50%-90% by mass, and the content of polyvinylpyrrolidone is 10%-50% by mass. The compression strength reaches 2 kPa or above and the resiliency reaches 90%-100% when the compression ratio of the IPN silk fibroin hydrogel is 60%. The provided silk fibroin hydrogel has the characteristics of high strength, elasticity and transparency and can be used as a polymer material for cornea repairing.

The invention belongs to the technical field of silk protein treatment and particularly relates to ionic liquid solution of regenerated silk protein and a preparation method thereof. The preparation method comprises a step of freeze-drying dewatering and a step of dissolving under reduced pressure and particularly comprises: uniformly mixing a silk or silk protein material and ionic liquid, freezing under a liquid nitrogen condition, and dewatering in a freezer dryer; and heating the dried mixture in an oil bath with stirring, removing air bubbles and possible residual water from the system under a reduced pressure to prepare the ionic liquid solution of the silk protein. The concentration of the final product is 1 to 35 weight percent, and the silk protein conformation in the solution ismainly random coil. In the invention, nontoxic, non-volatile and easily-recoverable ionic liquid is used as a solvent, so the method is quick, simple and easy; and the room-temperature storage performance under a water-free condition of the solution of the silk protein is high, and all physical and chemical indexes of the solution can be kept stable for a long time.

The invention discloses a soluble polyolefin resin and a preparation method thereof. The method comprises anyone of steps comprising: (a), polymerizing alpha-olefin under the action of a catalyst to polyolefin, and adding a solvent to the polyolefin to obtain the polyolefin resin; (b), polymerizing the alpha-olefin under the action of the catalyst and a disperse medium to obtain the polyolefin resin; and (c), polymerizing the alpha-olefin under the action of the catalyst and a disperse medium to obtain polyolefin, adding the solvent to the polyolefin, and removing the disperse medium to obtain the polyolefin resin. The solvent is introduced in the preparation of a polyolefin raw material process, so a subsequent dissolving technology is simplified, dissolving is accelerated, and the soluble polyolefin resin is formed. The introduction of the solvent into the above raw material effectively prevents polyolefin crystallization, maintains a random coil form, and increases the solubility of the polyolefin.

The invention discloses a freeze-dried powder of high molecular weight silk fibroin, preparation process and use thereof. The freeze-dried powder is obtained from silk by degumming, dissolution, dialysis, centrifugation, high temperature and high pressure treatment and freeze-drying. A method of preparing silk fibroin microspheres with polyethylene glycol comprises mixing a silk fibroin solution of 1-30 wt % with a PEG solution of 10-60 w % and incubating the resulting solution. A controlled-release or sustained-release silk fibroin gel formulation includes a gel-state carrier and a drug dispersed / adsorbed therein, the carrier is a silk fibroin gel formed by blending with LMW-PEG. The freeze-dried powder will not change the native molecular weight and random coil structure of silk fibroin, and can be rapidly dissolved into a solution, and can be stored and transported for a long time, and this reduces waste of silk fibroin materials and promotes R & D and biomedical applications of silk fibroin biomaterials.

The present invention relates to water-soluble protein carrier-linked prodrugs wherein the protein carrier comprises an amino acid sequence consisting of at least 100 amino acid residues forming random coil conformation and comprising alanine, serine and proline residues. It further relates to pharmaceutical compositions comprising said water-soluble protein carrier-linked prodrugs, their use as a medicament as well as methods of treatment and administration.

The invention discloses an active polypeptide capable of promoting osteogenesis and inhibiting osteoclast and application of the active polypeptide. N-terminal serine is phosphorylated on the basis ofPTH1-34, and three repeated sequences of glutamic acid or aspartic acid are introduced at a C terminal; the sequence of the polypeptide is shown in SEQ ID NO:1 or SEQ ID NO:2, and the polypeptide hasosteoinductive activity similar to BMP2 and an osteoclast inhibiting effect similar to parathyroid hormones (PTH). Random coils of the polypeptide can be avoided by bonding the C-terminal repeated sequences of the polypeptide to the surface of a calcium phosphate material or a material with a calcium phosphate coating, no addition of organic reagents is needed, and therefore the activity of the polypeptide is protected effectively; furthermore, slow controlled release can be achieved through cleavage of peptide bonds, and long-term intermittent injection of the PTH1-34 can be avoided, so thatthe injection pain of patients is reduced, and effects of osteoblast promotion and osteoporosis inhibition are achieved; a conventional viewpoint that the PTH1-34 cannot be administered locally is changed.

The invention discloses a vinyl alcohol-series polymer. The vinyl alcohol-series polymer has a core-arm star-shaped molecule structure comprising a core and vinyl alcohol-series polymer arms connected to the core. The vinyl alcohol-series polymer is characterized in that the arms are modified by ionic groups so that the vinyl alcohol-series polymer which has a star-shaped molecule structure comprising the ionic groups on the polymer arms and is shown in the general formula (I) is formed. The vinyl alcohol-series polymer is a macromolecule comprising a core in the center and multiple radiation linear polymer arms (which is vinyl alcohol-series polymer arms) connected to the core. Compared with linear and random coil polymers having corresponding molecular weights, the vinyl alcohol-series polymer has low viscosity in water. The polymer arms are modified by the ionic groups so that the polymer arms have iconicity. Through the iconicity characteristic, the vinyl alcohol-series polymer can be used as a paper surface sizing agent and a paper inner reinforcing agent for papermaking, a paper wet-part fine filler retention promoter, a waste water treatment flocculating agent, a sludge dehydrant and a crude oil dehydrant.

A polymer has a fluorescent dye sensitive to a hydrophobic environment. When a pH around the polymer decreases within a range of pH of equal to or greater than 5.5 and less than 7.4, and a secondary structure of the polymer changes from a random coil to a helix.

The invention belongs to the field of medical biological materials, and particularly discloses a modified type-I collagen, a modification method, and collagen gel prepared from the modified type-I collagen. The modification method comprises the steps of (1) uniformly mixing weak acid with a type-I collagen at the temperature of 2-10 DEG C to prepare an acid collagen solution; (2) utilizing a phosphate buffering solution for dialysing the acid collagen solution to obtain a neutral collagen solution; (3) removing bubbles in the neutral collagen solution; (4) utilizing a rotational rheometer forcutting the neutral collagen solution after bubble removal under a liquid seal condition, and conducting standing after cutting to prepare the modified type-I collagen. According to the modified type-I collagen, the modification method, and the collagen gel prepared from the modified type-I collagen, the collagen is modified through mechanical cutting. The method is simple and efficient, three-helix structures of collagen molecules can be reduced on the basis that other substances do not need to be introduced, and random coil structures are added, so that fibers of the assembled collagen are controllable in diameter, pore and viscoelasticity.

Disclosed are rigid random coils, having a contour length larger than a diameter thereof, an average bending ratio Db of 0.1˜0.8, and a weight average molecular weight of 1×108˜9×108 g / mole, and a resin composition or organic solvent composition including the same. The rigid random coils have the properties intermediate between those of polymers and rigid rod particles, and thus may be used as filler of various resins, aqueous solutions or organic solvents.

The invention discloses a spandex fiber with a shape memory effect and a preparation method of the spandex fiber. The spandex fiber is prepared from the following components: a crystalline polyether or polyester diol monomer, a diisocyanate monomer, a polyurethane chain extender, a polyurethane cross-linking agent and a peroxide initiator. The spandex material has a double cross-linking structure, the first cross-linking structure is a cross-linking network formed by the polyurethane cross-linking agent, and the second cross-linking agent is polyurethane secondary cross-linking initiated by decomposition of the peroxide initiator; when the temperature is higher than a melting temperature of a soft section of the fiber, an oriented crystal area in the fiber is shrunk into a random coil state due to crystallization melting, and the fiber length is shortened, so that a second cross-linking network is compressed; when the temperature of the fiber is lower than a crystallization melting temperature of the spandex, molten soft segment molecular chains in the fiber are subjected to oriented crystallization again under the action of tensile stress provided by the compressed second recross-linked network in the system, and it is shown that the length of the fiber is increased; and the above process has the characteristics of repeatability and reversibility along with the change of temperature.

The invention discloses a fusion tag protein. The fusion tag protein provided by the invention is constructed on the basis of C89S mutant (marked as HREV107N) of wild type HREV107 (1-125) protein, contains HREV107N the amino acid sequence of which is shown as SEQ ID No:1, two Linkers inserted into the P38-K57 random coil region of the mutant protein and multiple cloning sites corresponding short peptide between the two Linkers. The invention further provides a fusion protein expression system, which contains the nucleic acid molecule of the fusion tag protein. Atarget protein gene is inserted into the multiple cloning sites. The C terminal of the fusion tag protein can also be connected to rubredoxin tag protein and His tag so as to facilitate the expression and purification of the fusion protein.

The invention discloses photo-curable silk fibroin hydrogel and a preparation method thereof. The photo-curable silk fibroin hydrogel is prepared by: adding riboflavin and horse radish peroxidase intosilk fibroin solution that acts as a raw material, introducing oxygen continuously under ultraviolet irradiation and room temperature, inducing groups in silk fibroin macromolecules to chemically crosslink, and allowing gelling. The silk fibroin in the photo-curable hydrogel provided herein is mainly of random coil; the photo-curable hydrogel has high light transmittance, high compressive strength and high elasticity and is applicable to tissue repair materials.

This invention relates to novel recombinant clostridial neurotoxins exhibiting increased duration of effect and to methods for the manufacture of such recombinant clostridial neurotoxins. These novel recombinant clostridial neurotoxins comprise (i) at least two random coil domains, or (ii) at least two domains having a polyproline II helix conformation, and the methods comprise the steps of inserting at least two nucleic acid sequences each coding for (i) a random coil domain, or (ii) a domain having a polyproline II helix conformation, into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising (i) the random coil domain-coding sequences, or (ii) the sequences encoding the domains having a polyproline II helix conformation in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with increased duration of effect.

The invention discloses an electromagnetic heating coil plate, comprising a coil plate bracket provided with a plurality of coiling parts. A magnetic bar is arranged on the back surface of each coiling part; an interval cavity is reserved between the adjacent coiling parts; a plurality of baffle plates are arranged on the coiling parts; a coil groove is formed between the adjacent baffle plates; the coil grooves on the coiling parts commonly form a concentric circle structure; and a varnished wire is coiled on each coil groove and coils from one circle of the coil groove to the adjacent circle of the coil groove in any internal cavity. Accordingly, the coiling parts on the coil plate bracket can be arranged in a consistent structure, so as to greatly simplify the structure of the coil plate bracket and a structure of a preparation die. When a producer coils, the random coiling from one circle of the coil groove to the adjacent circle of the coil groove is realized in any one of the interval cavities between the coiling parts. The electromagnetic heating coil plate is flexible and convenient.

The invention discloses a freeze-dried powder of high molecular weight silk fibroin, preparation process and use thereof. The freeze-dried powder is obtained from silk by degumming, dissolution, dialysis, centrifugation, high temperature and high pressure treatment and freeze-drying. A method of preparing silk fibroin microspheres with polyethylene glycol comprises mixing a silk fibroin solution of 1-30 wt % with a PEG solution of 10-60 w % and incubating the resulting solution. A controlled-release or sustained-release silk fibroin gel formulation includes a gel-state carrier and a drug dispersed / adsorbed therein, the carrier is a silk fibroin gel formed by blending with LMW-PEG. The freeze-dried powder will not change the native molecular weight and random coil structure of silk fibroin, and can be rapidly dissolved into a solution, and can be stored and transported for a long time, and this reduces waste of silk fibroin materials and promotes R & D and biomedical applications of silk fibroin biomaterials.

This invention relates to novel recombinant clostridial neurotoxins exhibiting increased duration of effect and to methods for the manufacture of such recombinant clostridial neurotoxins. These novel recombinant clostridial neurotoxins comprise a random coil domain, and the methods comprise the steps of inserting a nucleic acid sequence coding for a random coil domain into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising the random coil domain-coding sequence in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with increased duration of effect.

The invention relates separation preparation and application of an Arcainflata Reeve oxidation-resistant active peptide. The separation preparation method comprises the following steps: peeling and cleaning Arcainflata Reeve, carrying out tissue homogenization, carrying out hydrolysis technique screening and optimization, centrifugating, taking the supernate, and carrying out freeze-drying to obtain an active polypeptide component; and sequentially carrying out ultrafiltration interception concentration, ion-exchange chromatography, gel filtration and high-efficiency exclusion chromatography on the active component to finally obtain the oxidation-resistant active polypeptide with the purity of 99%. Multiple modern protein purification techniques are creatively utilized to separate the active polypeptide with definite material basis from Arcainflata Reeve hydrolysate; the molecular weight of the active polypeptide is 17578.31Da; and the active polypeptide contains the following secondary structures: 40.8% of alpha-spiral, 24.7% of beta-fold, 15.9% of beta-corner and 18.6% of random coil. The polypeptide has obvious oxidation-resistant activity; the in-vitro removal DPPH, ABTS and hydroxy free radical IC50 values are respectively 3.89, 0.22 and 10.77 mg / ml; and the polypeptide can prolong the average life of the Caenorhabditis elegans by 18.29%. Therefore, the active peptide can be used for development and application of antioxidants, medicinal cosmetics or health products.

The invention provides menadione sodium bisulfite (MSB), namely a drug with the functions of preventing and treating Alzheimer's disease. It is found in research that menadione sodium bisulfite has the functions of obviously inhibiting aggregation of beta-amyloid protein1-42 (Abeta42), namely pathogenic protein of Alzheimer's disease, retarding conversion of a secondary structure of beta-amyloid protein1-42 from random coils into beta lamellas during aggregation, weakening the cytomembrane destruction capability of beta-amyloid protein1-42 toxicity congeries, inhibiting aggregation of beta-amyloid protein1-42 in cells, obviously prolonging the life of nematode in a caenorhabditis elegans AD model, achieving high safety performance and the like. It is disclosed that menadione sodium bisulfite can be applied to preparation of drugs with the functions of preventing and / or treating Alzheimer's disease.

This invention relates to novel recombinant clostridial neurotoxins exhibiting increased duration of effect and to methods for the manufacture of such recombinant clostridial neurotoxins. These novel recombinant clostridial neurotoxins comprise a random coil domain, and the methods comprise the steps of inserting a nucleic acid sequence coding for a random coil domain into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising the random coil domain-coding sequence in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with increased duration of effect.