The invention discloses an anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator is prepared from the following ingredients by weight: retinyl acetate, vitamin D3, d1-alpha-tocopheryl acetate, menadione, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, L-ascorbic acid, d-biotin, nicotinamide, pantothenic acid, folic acid, methionine, lysine, xylooligosaccharide, citric acid, sodium glutamate and glucose as balance. The invention also relates to a preparation method of the anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator prepared by the method can stimulate the self-metabolism regulation, can improve the feed utilization rate, can improve the food consumption of the growing-finishing pigs and the reproductive performance of the sows, can improve the anti-stress ability, can reduce the death rate and the diarrhea occurrence rate of piglets, has a remarkable growth promotion effect, can improve the growth speed of the weaned pigs, the growing-finishing pigs and the suckling pigs, and has remarkable economic benefit.