40 results about "Menadione" patented technology

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The application provides a kind of aromatic isopentenyl transferase mutant, which is based on the aromatic isopentenyl transferase gene MenA derived from Bacillus subtilis ( Bacillus subtilis ) and six aromatic isopentenyl transferase mutants MenA-1 to MenA-6 are obtained by site-directed mutagenesis PCR technology, and the recombinant bacteria Bacillus subtilis expressing the above mutants are constructed, respectively. The yield of heptamethylnaphthoquinone fermented by the recombinant bacteria is 64.49 mg / L, 69.30 mg / L, 83.86 mg / L, 105.84 mg / L, 119.23 mg / L and 125.45 mg / L, respectively, which is 133%, 143%, 173%, 219%, 246% and 259% of the yield of heptamethylnaphthoquinone fermented by the Bacillus subtilis expressing the wild-type aromatic isopentenyl transferase MenA.

The invention discloses a novel crystal form of menadione and a preparation method, and relates to the technical field of organic chemistry. The novel crystal form comprises a crystal form T, and the crystal form T has diffraction peaks when the 2theta value is 9.970 + / -0.2 degrees, 12.250 + / -0.2 degrees, 14.978 + / -0.2 degrees and 19.141 + / -0.2 degrees. The preparation method of the novel crystal form comprises the following steps: dissolving a menadione crude product in a mixed solvent composed of an ester solvent and an alcohol solvent, cooling to 10-20 DEG C, and crystallizing to obtain the menadione crystal form T; the menadione crystal form T obtained through the method is a novel crystal form, the crystal form is good in stability and solubility, a solvent system is environmentally friendly, solvent residues are very small, the risk is low, the medication safety is better guaranteed, the defects of an existing crystal form can be overcome, and high economic benefits and social benefits are achieved.

The invention discloses a filamentous fungus genetic transformation system taking an endogenous KatG1 gene as a selection marker, a method and application. The method comprises the following steps: carrying out linear DNA fragment construction on an endogenous Pgpd promoter, an endogenous KatG1 gene, a target promoter and a target gene of filamentous fungi, then transforming the constructed linear DNA fragment into filamentous fungi cells, and finally screening through a menadione-containing culture medium to obtain the directionally improved filamentous fungi strain containing the target gene. According to the invention, the endogenous KatG1 gene of the fungus is used as a selection marker gene and menadione is used as screening resistance to construct a novel selection marker based on an endogenous gene and a genetic transformation system without exogenous DNA (Deoxyribose Nucleic Acid) such as a carrier and the like, so that the method has the advantages of universality, high efficiency and safety; an environment-friendly solution is provided for directional genetic improvement of filamentous fungi, and the method has important commercial application potential in directional breeding of fungus strains in the fields of agriculture, industry and the like.

The present application discloses compositions comprising nanoparticles of vitamin K2, and their methods of use.

The invention provides application of a composition containing butyric acid producing bacteria and vitamin K2 in preparation of a medicine for treating osteoporosis, and relates to the technical field of biological medicines, the composition comprises butyric acid producing bacteria and vitamin K2; wherein the butyric acid producing bacteria are selected from any one or more of clostridium butyricum, rosella and clostridium praeparatum; and the vitamin K2 is selected from any one or more of menadione-4 or menadione-7. The composition can play a synergistic effect through an intestine-bone axis, and compared with a single component, the composition can more remarkably improve the bone mineral density. The composition has comprehensive effects and high safety, provides a brand-new nutrition intervention strategy with a synergistic effect for prevention and treatment of osteoporosis, and provides a brand-new possibility for preparation of medicines related to osteoporosis.

The invention discloses a method for improving the synthesis yield of vitamin K3, which comprises the following steps: S1, adding beta-menadione and sodium pyrosulfite into a container, adding ethanol as a solvent, stirring to react for 1-2 hours at the reaction temperature of 30-50 DEG C, heating to 40-70 DEG C, keeping the temperature for 10-60 minutes, and filtering while hot after the reaction is completed to obtain filtrate; and S2, adding sodium pyrosulfite into the filtrate, and then freezing, crystallizing, filtering, washing and drying to obtain a vitamin K3 finished product. When the vitamin K3 is prepared through cooling crystallization, the sodium pyrosulfite is added into the filtrate to be crystallized, so that the crystallization yield of the vitamin K3 can be effectively improved.

The invention relates to the technical field of menadione intermediate 1 preparation, and discloses a menadione intermediate 1 preparation method, which comprises: 1, introducing dicyclopentadiene into a first continuous flow reactor, and carrying out a depolymerization reaction; step 2, synchronously introducing cyclopentadiene in the step 1 and a solution containing menadione and a Lewis acid catalyst into a second continuous flow reactor; step 3, introducing the reaction liquid in the step 2 and water into a third continuous flow reactor for continuous quenching and liquid separation, and separating an organic phase; 4, drying the organic phase, concentrating, pulping with methanol, and purifying to obtain the menadione intermediate 1; the continuous flow reactor is adopted to realize continuous quenching and continuous liquid separation, the operation risk is reduced while the operation is simplified, the production safety is improved, the Lewis acid is adopted as the catalyst, the reaction process is mild in condition, small in acid consumption, short in reaction time and small in corrosion to equipment, and the three-waste pollution is greatly reduced.

The present application relates to a kind of preparation methods of sodium bisulfite menadione, which is prepared by the reaction of 2-methyl-1,4-naphthoquinone and sodium bisulfite in the presence of K2O-MgO / Al2O3 as an aid in the mixed solution of organic solvent and water.The present application makes the conversion rate of raw materials >99% by introducing K2O-MgO / Al2O3 as an aid, the selectivity is greater than 95%, and can reduce the phenol, quinone impurities (content <800ppm) in the product.The process is simple, the reaction condition is mild, the process is stable, the purity of product is more than 98%, and the total yield is more than 94%.

This invention discloses a desmethylmenoprenone methyltransferase mutant, its construction method, and its applications, belonging to the field of biotechnology. Desmethylmenoprenone methyltransferase is a key enzyme in the MK-7 biosynthetic pathway, responsible for catalyzing the methylation reaction of desmethylmenoprenone to generate a series of methylnaphthoquinone (MK) products, ultimately forming MK-7. This invention, through metabolic network regulation and rational design, successfully obtained 19 single-point mutants, among which 5 mutants showed significantly higher catalytic efficiency than the wild-type enzyme. Experimental results show that the recombinant engineered bacteria constructed based on these mutants exhibit significant advantages in the whole-cell catalytic production of vitamin K2 (MK-7), with MK-7 yield increased by more than 20% compared to the wild-type strain. The excellent catalytic performance of the menG mutant provided by this invention provides strong technical support for the industrial production of vitamin K2, and has broad application prospects and market value.

The invention discloses a method for accelerating the color development speed of filamentous fungus resazurin and application of the method, and relates to the technical field of drug sensitivity detection. The invention provides a method for accelerating the color development speed of filamentous fungus resazurin, which comprises the following steps: S1, diluting a filamentous fungus bacterial liquid by using an MUELLER-HINTON culture medium, and adding the diluted filamentous fungus bacterial liquid into a to-be-detected drug hole for incubation; s2, performing suction filtration after incubation; s3, adding a color developing agent containing a catalyst for color development; s4, result interpretation; the catalyst in the step S3 is menadione. According to the MUELLER-HINTON culture medium, in the Aspergillus drug sensitivity detection process based on resazurin color development, after a catalyst is added, color development is accelerated, meanwhile, all medicine adding holes cannot be red, and the MIC value can be accurately obtained.

The invention is applicable to the technical field of feeds, and provides a special nutrient enrichment premix for boars and a preparation method thereof. The compound feed additive is prepared by compounding beta-carotene, 25-hydroxyvitamin D3, dl-alpha-tocopheryl acetate, menadione sodium hydrogen sulfite, ammonium sulfate nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamin, nicotinamide, D-calcium pantothenate, folic acid, D-biotin, L-ascorbic acid, DL-methionine, zinc methionine chelate, selenium yeast, chromium picolinate, L-carnitine and glucose according to a specific proportion. A dynamic batching and high-precision mixing process is adopted for preparation. Through the synergistic effect of various nutrients, the sow milk quality can be remarkably improved, the birth weight of piglets is increased by 50 g or above, the survival rate is increased by 6-8% or above, the weaning weight is increased by 500 g or above, meanwhile, the sow estrus rate is increased by 5% or above, the problems that sows are poor in body condition, low in reproductive performance, low in piglet survival rate and the like in existing boar breeding are effectively solved, and extremely high breeding application value is achieved.

Compositions comprising nanoparticles of vitamin K2 and methods of use thereof are disclosed.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

This disclosure provides a method for alleviating aluminum toxicity in hydroponic peas based on menadione-mediated reactive oxygen species (ROS) metabolism. The core components include: regulating the concentration and duration of menadione treatment to optimize the spatiotemporal accumulation pattern of ROS (superoxide anion, hydrogen peroxide) in the roots of hydroponic peas; adjusting the dynamic response of endogenous antioxidant enzyme systems (SOD, POD, APX) to enhance menadione-mediated ROS scavenging capacity; controlling the initial ROS burst based on changes in plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity to inhibit the aluminum stress oxidative signaling cascade; and adjusting the timing and frequency of menadione administration based on subcellular-scale mitochondrial and chloroplast ROS leakage rates to alleviate organ-specific oxidative damage. This approach addresses five key issues: aluminum-induced oxidative damage, cellular redox imbalance, oxidative signaling cascade, organ-specific oxidative damage, and persistent aluminum toxicity metabolic disorders.

The invention provides methods and compositions for preventing or treating (e.g., slowing the progression of, arresting, and / or reversing) tissue calcification in a subject in need thereof and, more particularly, the invention relates to methods of using menaquinone-7 (MK-7) and / or menaquinol-7 (MKH2-7) for preventing or treating (e.g., slowing the progression of, arresting, and / or reversing) tissue calcification in a subject with diabetes, chronic kidney disease, end stage renal failure, or a subject undergoing hemodialysis and / or receiving anticoagulant therapy. The invention further provides methods and compositions for reducing one or more symptoms of chronic obstructive pulmonary disorder (COPD), including using menaquinone-7 (MK-7) and / or menaquinol-7 (MKH2-7), for preventing or treating (e.g., slowing the progression of, arresting, and / or reversing) one or more symptoms of COPD.

The invention relates to a hair growth medicine and application thereof in preparation of a medicine for preventing and treating male hormone alopecia, and belongs to the technical field of biological medicines. The invention discloses an application of menadione in preparation of a hair growth medicament. According to the present invention, the drugs capable of effectively treating male hormone alopecia are screened by using the high throughput screening method, comprise menthone, menadione and caffeic acid, and can significantly increase the hair follicle number, the hair follicle length and the corium layer thickness of the skin, the composition is used for treating male hormone alopecia, effectively promotes hair growth, and has no obvious side effects, and the expression quantity of the hair growth promoting gene is increased, and the expression quantity of the hair growth inhibiting gene is reduced.

The application provides a kind of aromatic isopentenyl transferase mutant, to be derived from Bacillus subtilis ( Bacillus subtilis )SEPHCHC synthase gene MenD is based, six aromatic isopentenyl transferase mutants MenD-1, MenD-2, MenD-3, MenD-4, MenD-5 and MenD-6 are obtained by site-directed mutagenesis PCR technology, and the recombinant bacteria Bacillus subtilis expressing the above mutants is respectively constructed, and the yield of heptamethylnaphthoquinone fermentation is 70.77 mg / L, 75.48 mg / L, 81.72 mg / L, 88.41 mg / L, 99.88 mg / L and 133.40 mg / L respectively, which is 116%, 123%, 134%, 144%, 162% and 218% of the yield of heptamethylnaphthoquinone of Bacillus subtilis expressing wild-type SEPHCHC synthase MenD respectively.

The present application relates to 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic acid synthase mutant and its application, and belongs to the technical field of enzyme engineering. The present application obtains key residues of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic acid synthase by combining protein sequence comparison and alanine scanning strategy, randomly mutates the key residues, and obtains a mutant MenD which is resistant to feedback inhibition of 1,4-dihydroxy-2-naphthalene carboxylic acid intermediate metabolite in the methionine quinone pathway through multiple rounds of screening W322I\R323F The ability of the recombinant bacillus subtilis to produce heptamethylnaphthoquinone is improved, and the yield is 1.22 times that of the control strain, and the final yield reaches 186.5 mg / L. The present application not only provides a new direction for the modification of the methionine quinone pathway of bacillus subtilis, but also will be beneficial to the industrial large-scale production of heptamethylnaphthoquinone.

The invention provides a 1, 4-dihydroxy-2-naphthoate polypentenyl transferase mutant, which is characterized in that on the basis of a 1, 4-dihydroxy-2-naphthoate polypentenyl transferase gene MenA derived from bacillus subtilis, 13 1, 4-dihydroxy-2-naphthoate polypentenyl transferase mutants are obtained through a site-directed mutagenesis PCR (Polymerase Chain Reaction) technology, and the 1, 4-dihydroxy-2-naphthoate polypentenyl transferase gene MenA is used as a template for preparing the 1, 4-dihydroxy-2-naphthoate polypentenyl transferase mutant. Recombinant bacteria bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutants are respectively constructed, and compared with bacillus subtilis heptaene menadione for expressing wild type 1, 4-dihydroxy-2-naphthoate polypentenyl transferase MenA, the yield of the heptaene menadione fermented by the recombinant bacteria is respectively improved by 7%-258%.

The present application discloses compositions comprising nanoparticles of vitamin K2, and their methods of use.

The invention relates to the technical field of agricultural protection, in particular to application of naphthoquinone compounds in prevention and treatment of plant wilt diseases. The naphthoquinone compound is menadione or menadione tetraenone. According to the application, the naphthoquinone compound serves as an active substance to be prepared into a medicament, and the medicament is applied to the surfaces or roots of diseased plants so as to prevent and control plant wilt diseases caused by fusarium oxysporum. The menadione tetraenone and menadione have a remarkable inhibiting effect on fusarium oxysporum which is a fusarium wilt pathogenic bacterium, and a novel natural-source and synthetic-source bactericidal active substance is provided for preventing and treating the plant fusarium wilt. The menadione tetraenone and menadione are efficient, low in toxicity and environment-friendly, and meet the development requirements of current green pesticides.

Compositions comprising nanoparticles of vitamin K2 and methods of use thereof are disclosed.

The invention provides a method for determining the content and the content uniformity of menadione sodium bisulfite powder (for aquatic products) by an RP-HPLC (Reverse Phase-High Performance Liquid Chromatography) method, and belongs to the technical field of veterinary drug detection. The method is high in precision, good in accuracy, strong in specificity, good in stability and simple and convenient to operate. Compared with the prior art, the detection method has the advantages that the content and the uniformity of menadione sodium hydrogen sulfite can be more accurately detected, the production quality and the stability of products can be effectively controlled, and the product quality and the market competitiveness are improved.

This invention discloses albumin nanoparticles co-loaded with flavin and lactate oxidase, their preparation method, and applications. Using human serum albumin as a carrier, flavin and lactate oxidase are simultaneously encapsulated at a mass ratio of 10:1, with a particle size of 150-180 nm and a polydispersity index of less than 0.2. This invention exerts its anti-hepatocellular carcinoma effect through mechanisms such as reducing lactate levels in the tumor microenvironment, inducing an increase in reactive oxygen species, inhibiting cGAS protein lactation modification, and activating the cGAS-STING signaling pathway. The nanoparticles exhibit high encapsulation efficiency and good stability. The flavin loaded on these nanoparticles can significantly reduce the reduction of pyruvate to lactate by inhibiting lactate dehydrogenase; simultaneously, the lactate oxidase loaded on these nanoparticles catalyzes the dehydrogenation of lactate to pyruvate. In other words, these nanoparticles synergistically regulate lactate metabolism through a two-way regulatory strategy of "reducing source and increasing sink"—"inhibiting lactate production" and "promoting lactate clearance"—significantly enhancing in vitro and in vivo anti-tumor effects, and demonstrating high biosafety.

The present application discloses compositions comprising nanoparticles of vitamin K2, and their methods of use.

The invention discloses a method for synthesizing high-value chemicals through enzyme catalysis aromatic hydrocarbon dearomatization. According to the method, in a water phase system, 2-methylnaphthalene is taken as a substrate, hydrogen peroxide is taken as an oxidant, ascorbic acid is taken as a free radical scavenger, and a non-specific peroxygenase mutant is taken as a catalyst, so that an efficient catalytic synthesis way is established. The non-specific peroxygenase mutant is obtained by carrying out rational design modification on the non-specific peroxygenase, including mutation on a 99th or 119th amino acid residue point or truncation on a C terminal, so that the tolerance and catalytic efficiency of the enzyme to a high-concentration aromatic hydrocarbon substrate are improved. Efficient synthesis of menadione can be achieved through a green and mild biological enzyme method, and a key enzyme tool is provided for industrial biological manufacturing of vitamin K3.

This application discloses compositions comprising vitamin K2 nanoparticles and methods of using the same. In one embodiment, a composition is provided comprising water-soluble vitamin K2 nanoparticles (or nano-sized particles), wherein the nanoparticles have an average particle size of about 0.1 nm to 1,000 nm. In one embodiment, the vitamin K2 is MK-9 (menaquinone-9) and / or MKH2-9 (menaquinol-9). In another embodiment, the nanoparticles have an average particle size of 200 nm, 175 nm, 150 nm, 125 nm, 115 nm, 100 nm, 90 nm, less than 80 nm, or less than 75 nm. In another embodiment, the composition is a stable composition.

The present invention relates to the technical field of pharmaceutical preparations, and discloses a menadione soft capsule and a preparation method thereof, the capsule comprises a capsule shell and a content filled in the capsule shell, the content comprises the following substances by weight: 12%-20% of menadione, 25%-38% of a surfactant, and the balance of an oily solvent; the capsule shell comprises the following substances in percentage by weight: 30%-35% of a film-forming agent, 12.75%-17% of a plasticizer, 15%-18% of an enteric-coated material, 2%-4% of a metal ion chelating agent, 0.1%-1.0% of a pH regulator, 0.2%-0.8% of an opacifying agent, 0.05%-0.1% of a coloring agent and the balance of purified water; the metal ion chelating agent is aspartic acid; the film-forming agent is a composition of sodium alginate and gelatin. According to the scheme, the menadione enteric soft capsule has enteric solubility and excellent preparation property, can be dissolved in acid under the condition that the menadione enteric soft capsule is not dissolved in weak base, and is beneficial to improving absorption of menadione in small intestines, so that the bioavailability is improved; and the crosslinking phenomenon in the long-term storage process can be reduced, so that the long-term storage of the product is facilitated.