20 results about "Menadione" patented technology

The application provides a kind of aromatic isopentenyl transferase mutant, which is based on the aromatic isopentenyl transferase gene MenA derived from Bacillus subtilis ( Bacillus subtilis ) and six aromatic isopentenyl transferase mutants MenA-1 to MenA-6 are obtained by site-directed mutagenesis PCR technology, and the recombinant bacteria Bacillus subtilis expressing the above mutants are constructed, respectively. The yield of heptamethylnaphthoquinone fermented by the recombinant bacteria is 64.49 mg / L, 69.30 mg / L, 83.86 mg / L, 105.84 mg / L, 119.23 mg / L and 125.45 mg / L, respectively, which is 133%, 143%, 173%, 219%, 246% and 259% of the yield of heptamethylnaphthoquinone fermented by the Bacillus subtilis expressing the wild-type aromatic isopentenyl transferase MenA.

The invention provides application of a composition containing butyric acid producing bacteria and vitamin K2 in preparation of a medicine for treating osteoporosis, and relates to the technical field of biological medicines, the composition comprises butyric acid producing bacteria and vitamin K2; wherein the butyric acid producing bacteria are selected from any one or more of clostridium butyricum, rosella and clostridium praeparatum; and the vitamin K2 is selected from any one or more of menadione-4 or menadione-7. The composition can play a synergistic effect through an intestine-bone axis, and compared with a single component, the composition can more remarkably improve the bone mineral density. The composition has comprehensive effects and high safety, provides a brand-new nutrition intervention strategy with a synergistic effect for prevention and treatment of osteoporosis, and provides a brand-new possibility for preparation of medicines related to osteoporosis.

The invention discloses a method for improving the synthesis yield of vitamin K3, which comprises the following steps: S1, adding beta-menadione and sodium pyrosulfite into a container, adding ethanol as a solvent, stirring to react for 1-2 hours at the reaction temperature of 30-50 DEG C, heating to 40-70 DEG C, keeping the temperature for 10-60 minutes, and filtering while hot after the reaction is completed to obtain filtrate; and S2, adding sodium pyrosulfite into the filtrate, and then freezing, crystallizing, filtering, washing and drying to obtain a vitamin K3 finished product. When the vitamin K3 is prepared through cooling crystallization, the sodium pyrosulfite is added into the filtrate to be crystallized, so that the crystallization yield of the vitamin K3 can be effectively improved.

The present application relates to a kind of preparation methods of sodium bisulfite menadione, which is prepared by the reaction of 2-methyl-1,4-naphthoquinone and sodium bisulfite in the presence of K2O-MgO / Al2O3 as an aid in the mixed solution of organic solvent and water.The present application makes the conversion rate of raw materials >99% by introducing K2O-MgO / Al2O3 as an aid, the selectivity is greater than 95%, and can reduce the phenol, quinone impurities (content <800ppm) in the product.The process is simple, the reaction condition is mild, the process is stable, the purity of product is more than 98%, and the total yield is more than 94%.

This invention discloses a desmethylmenoprenone methyltransferase mutant, its construction method, and its applications, belonging to the field of biotechnology. Desmethylmenoprenone methyltransferase is a key enzyme in the MK-7 biosynthetic pathway, responsible for catalyzing the methylation reaction of desmethylmenoprenone to generate a series of methylnaphthoquinone (MK) products, ultimately forming MK-7. This invention, through metabolic network regulation and rational design, successfully obtained 19 single-point mutants, among which 5 mutants showed significantly higher catalytic efficiency than the wild-type enzyme. Experimental results show that the recombinant engineered bacteria constructed based on these mutants exhibit significant advantages in the whole-cell catalytic production of vitamin K2 (MK-7), with MK-7 yield increased by more than 20% compared to the wild-type strain. The excellent catalytic performance of the menG mutant provided by this invention provides strong technical support for the industrial production of vitamin K2, and has broad application prospects and market value.

The invention discloses a method for accelerating the color development speed of filamentous fungus resazurin and application of the method, and relates to the technical field of drug sensitivity detection. The invention provides a method for accelerating the color development speed of filamentous fungus resazurin, which comprises the following steps: S1, diluting a filamentous fungus bacterial liquid by using an MUELLER-HINTON culture medium, and adding the diluted filamentous fungus bacterial liquid into a to-be-detected drug hole for incubation; s2, performing suction filtration after incubation; s3, adding a color developing agent containing a catalyst for color development; s4, result interpretation; the catalyst in the step S3 is menadione. According to the MUELLER-HINTON culture medium, in the Aspergillus drug sensitivity detection process based on resazurin color development, after a catalyst is added, color development is accelerated, meanwhile, all medicine adding holes cannot be red, and the MIC value can be accurately obtained.

Compositions comprising nanoparticles of vitamin K2 and methods of use thereof are disclosed.

There are described compositions comprising an effective amount of a combination of two or more components, said components selected from acacetin, ACTI peptide, alpha-lipolic acid, alprostadil, anisomycin, apigenin, ascorbic acid, astragalus, berberine, β-lapachone, β-hydroxy-beta-methyl-butyrate, Bacopa monnieri, catechin, catechol, chamomile, chrysin, coumestrol, curcumin, dinitrophenol, dinoprost, ellagic acid, (−)-epigallocatechin gallate, green tea extract, fisetin, genistein, ginsenoside RE, glabridin, 18-α-glycyrrhetinic acid, 18-β-glycyrrhetinic acid, glycyrrhizin, hydroquinone, isoquercitrin (EMIQ), kaempferol, kuromanin, leucine, lithium, luteolin, luteolin, luteolinidin, melatonin, menadione, 1-methylnicotinamide (MNA), methyl salicylate, myricetin, nadide, niacin (vitamin B3), nicotinamide (NAM), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid mononucleotide (NaMN), parsley (Petroselinium crispum), phenylephrine, pokeweed mitogen, 15-Δ prostaglandin J2, puromycin, quercetin, quinolinic acid, retinoic acid, trichostatin A, troxrutin, rutin, tryptophan, vitamin D3, withaferin A, wortmannin and zinc (including salts thereof).

This disclosure provides a method for alleviating aluminum toxicity in hydroponic peas based on menadione-mediated reactive oxygen species (ROS) metabolism. The core components include: regulating the concentration and duration of menadione treatment to optimize the spatiotemporal accumulation pattern of ROS (superoxide anion, hydrogen peroxide) in the roots of hydroponic peas; adjusting the dynamic response of endogenous antioxidant enzyme systems (SOD, POD, APX) to enhance menadione-mediated ROS scavenging capacity; controlling the initial ROS burst based on changes in plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity to inhibit the aluminum stress oxidative signaling cascade; and adjusting the timing and frequency of menadione administration based on subcellular-scale mitochondrial and chloroplast ROS leakage rates to alleviate organ-specific oxidative damage. This approach addresses five key issues: aluminum-induced oxidative damage, cellular redox imbalance, oxidative signaling cascade, organ-specific oxidative damage, and persistent aluminum toxicity metabolic disorders.

The application provides a kind of aromatic isopentenyl transferase mutant, to be derived from Bacillus subtilis ( Bacillus subtilis )SEPHCHC synthase gene MenD is based, six aromatic isopentenyl transferase mutants MenD-1, MenD-2, MenD-3, MenD-4, MenD-5 and MenD-6 are obtained by site-directed mutagenesis PCR technology, and the recombinant bacteria Bacillus subtilis expressing the above mutants is respectively constructed, and the yield of heptamethylnaphthoquinone fermentation is 70.77 mg / L, 75.48 mg / L, 81.72 mg / L, 88.41 mg / L, 99.88 mg / L and 133.40 mg / L respectively, which is 116%, 123%, 134%, 144%, 162% and 218% of the yield of heptamethylnaphthoquinone of Bacillus subtilis expressing wild-type SEPHCHC synthase MenD respectively.

The invention provides a method for determining the content and the content uniformity of menadione sodium bisulfite powder (for aquatic products) by an RP-HPLC (Reverse Phase-High Performance Liquid Chromatography) method, and belongs to the technical field of veterinary drug detection. The method is high in precision, good in accuracy, strong in specificity, good in stability and simple and convenient to operate. Compared with the prior art, the detection method has the advantages that the content and the uniformity of menadione sodium hydrogen sulfite can be more accurately detected, the production quality and the stability of products can be effectively controlled, and the product quality and the market competitiveness are improved.

This invention discloses albumin nanoparticles co-loaded with flavin and lactate oxidase, their preparation method, and applications. Using human serum albumin as a carrier, flavin and lactate oxidase are simultaneously encapsulated at a mass ratio of 10:1, with a particle size of 150-180 nm and a polydispersity index of less than 0.2. This invention exerts its anti-hepatocellular carcinoma effect through mechanisms such as reducing lactate levels in the tumor microenvironment, inducing an increase in reactive oxygen species, inhibiting cGAS protein lactation modification, and activating the cGAS-STING signaling pathway. The nanoparticles exhibit high encapsulation efficiency and good stability. The flavin loaded on these nanoparticles can significantly reduce the reduction of pyruvate to lactate by inhibiting lactate dehydrogenase; simultaneously, the lactate oxidase loaded on these nanoparticles catalyzes the dehydrogenation of lactate to pyruvate. In other words, these nanoparticles synergistically regulate lactate metabolism through a two-way regulatory strategy of "reducing source and increasing sink"—"inhibiting lactate production" and "promoting lactate clearance"—significantly enhancing in vitro and in vivo anti-tumor effects, and demonstrating high biosafety.

The invention discloses a method for synthesizing high-value chemicals through enzyme catalysis aromatic hydrocarbon dearomatization. According to the method, in a water phase system, 2-methylnaphthalene is taken as a substrate, hydrogen peroxide is taken as an oxidant, ascorbic acid is taken as a free radical scavenger, and a non-specific peroxygenase mutant is taken as a catalyst, so that an efficient catalytic synthesis way is established. The non-specific peroxygenase mutant is obtained by carrying out rational design modification on the non-specific peroxygenase, including mutation on a 99th or 119th amino acid residue point or truncation on a C terminal, so that the tolerance and catalytic efficiency of the enzyme to a high-concentration aromatic hydrocarbon substrate are improved. Efficient synthesis of menadione can be achieved through a green and mild biological enzyme method, and a key enzyme tool is provided for industrial biological manufacturing of vitamin K3.

This application discloses compositions comprising vitamin K2 nanoparticles and methods of using the same. In one embodiment, a composition is provided comprising water-soluble vitamin K2 nanoparticles (or nano-sized particles), wherein the nanoparticles have an average particle size of about 0.1 nm to 1,000 nm. In one embodiment, the vitamin K2 is MK-9 (menaquinone-9) and / or MKH2-9 (menaquinol-9). In another embodiment, the nanoparticles have an average particle size of 200 nm, 175 nm, 150 nm, 125 nm, 115 nm, 100 nm, 90 nm, less than 80 nm, or less than 75 nm. In another embodiment, the composition is a stable composition.

The present invention relates to the technical field of pharmaceutical preparations, and discloses a menadione soft capsule and a preparation method thereof, the capsule comprises a capsule shell and a content filled in the capsule shell, the content comprises the following substances by weight: 12%-20% of menadione, 25%-38% of a surfactant, and the balance of an oily solvent; the capsule shell comprises the following substances in percentage by weight: 30%-35% of a film-forming agent, 12.75%-17% of a plasticizer, 15%-18% of an enteric-coated material, 2%-4% of a metal ion chelating agent, 0.1%-1.0% of a pH regulator, 0.2%-0.8% of an opacifying agent, 0.05%-0.1% of a coloring agent and the balance of purified water; the metal ion chelating agent is aspartic acid; the film-forming agent is a composition of sodium alginate and gelatin. According to the scheme, the menadione enteric soft capsule has enteric solubility and excellent preparation property, can be dissolved in acid under the condition that the menadione enteric soft capsule is not dissolved in weak base, and is beneficial to improving absorption of menadione in small intestines, so that the bioavailability is improved; and the crosslinking phenomenon in the long-term storage process can be reduced, so that the long-term storage of the product is facilitated.

The main purpose of the present inventors is to provide a technique for suppressing inflammation, in particular, in aging tissues within the oral cavity.　The present inventors have found that an oral composition containing at least one selected from the group consisting of β-glycyrrhetinic acid, glycyrrhizic acid, ascorbic acid, tocopherol nicotinate, tocopherol acetate, pyridoxine, cobalamin, retinol, retinol acetate, riboflavin, phylloquinone, calciferol, anthraquinone, menaquinone-4, and L-aspartic acid, and chemically acceptable salts thereof, quercetin, genistein, fisetin, nobiletin, kaempferol, apigenin, and naringenin, and glycosides thereof, and methyl hesperidin can suppress inflammation, in particular, in aging tissues within the oral cavity.

The application provides a method for biosynthesis of tetraenemenaquinone by Yarrowia lipolytica. The method is achieved by constructing recombinant Yarrowia lipolytica, and the construction method of the recombinant Yarrowia lipolytica for synthesizing tetraenemenaquinone comprises the following steps: intensively expressing a mevalonate pathway in Yarrowia lipolytica, and introducing a heterologous synthesis pathway of geranylgeranyl pyrophosphate to provide sufficient isoprene side chain donors for the biosynthesis of tetraenemenaquinone; on the basis, overexpressing a codon-optimized aromatic isopentenyltransferase gene with menaquinone substrate specificity in Yarrowia lipolytica. The construction method of Yarrowia lipolytica for biosynthesis of tetraenemenaquinone provided by the application is simple in operation, the constructed Yarrowia lipolytica can obtain a high-concentration tetraenemenaquinone product by using commercially available menaquinone as a substrate, the biosynthesis steps are short, and the method has high production application value.

The present application discloses compositions comprising nanoparticles of vitamin K2, and their methods of use.

The invention provides bacillus subtilis engineering bacteria with high yield of heptaene menadione as well as a construction method and application of the bacillus subtilis engineering bacteria, and belongs to the technical field of genetic engineering. The method comprises the following steps: by taking bacillus subtilis 168 as an original strain, firstly, improving the transcriptional level of fni and isph genes, enhancing the side chain precursor flux and knocking out a competitive branch; furthermore, substrate binding pockets of key enzymes IspA and HepT are subjected to directed evolution, and the synthesis efficiency of MK-7 is optimized; the invention also optimizes the fermentation process of the engineering bacterium, and realizes efficient synthesis and extraction of MK-7. According to the engineering bacterium, the total MK-7 yield of the heptaene menadione strain in a fermentation product can reach 95.6 mg / L and is increased by about 3.1 times compared with that of an initial engineering bacterium, the proportion of heptaene menadione is increased, and the subsequent separation and purification cost is remarkably reduced.