The invention discloses a stable, efficient and environment-friendly
silver nanoparticle lactic acid bacteria biosynthesis method and application, firstly, a
nitrate reduction test is carried out to determine the ability of strains to reduce
nitrate into
nitrite, and optimized strains are screened from six
lactic acid bacteria; then, a response surface method is used for optimizing culture medium components for
nitrate reductase production, and synthesis conditions are optimized; and synthesizing the silver nanoparticles by using the screened optimized strains. Compared with physical and chemical methods, the
lactobacillus synthesis of AgNPs has the advantages of
cost effectiveness, environmental friendliness,
low toxicity to human cells, easiness in large-scale production, no need of dangerous chemicals, high temperature and
high pressure and the like. The invention provides an efficient, stable and environment-friendly method for biologically synthesizing silver nanoparticles by using
lactic acid bacteria, the improvement of antibacterial packaging materials is a key strategy and method for controlling microorganisms of chilled meat
putrefaction in China, and powerful
technical support is provided for scientifically, systematically, efficiently and practically guaranteeing meat safety.