The invention discloses a compound biological enzyme deodorant and a preparation method thereof. The compound biological enzyme deodorant contains the ingredients consisting of one of laccase, hemicellulase and oxidoreductase and the mixture of proteinase, lipase, cellulose, pectinase and a filler and is prepared by carrying out the following working procedures of: enzyme selecting and compounding, standing and nitrification, low-temperature concentrating, molecular sieving, hierarchal filtering, fluidized bed low-temperature drying, molecular modification and immobilization, product forming,inspecting and packaging. The product prepared by the preparation method has the advantages of stable quality, remarkable deodorizing effect, longer keeping time, environment-friendliness, convenience in use and suitability for the air purification and deodorization of fields, such as the municipal facilities including public toilets, garbage dump stations, garbage transfer stations and the like,the public places including stations, guest houses, hotels and the like, the household facilities including kitchens, wash basins, shower compartments, siphon tubes or any drainage pipeline and the like, and the factory workshops and the like.