Preparation of crust oligosaccharide and use

A chitooligosaccharide and chitosan technology, which is applied in the field of preparation of chitooligosaccharides, can solve the problems of imperfect process, low product yield, increased production cost, etc., and achieve the improvement of chitin industry level and technical content High, promote the effect of development

Inactive Publication Date: 2003-06-25
上海华宝孔雀香精有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still some difficulties in applying these non-specific hydrolytic enzymes to industrialized production of chitosan: one is that the degree of hydrolysis of chitosan by non-specific enzymes is limited. The amount of use is large (generally above 20%, and the enzyme-to-substrate ratio reaches 0.2-0.3: 1), on the other hand, the product yield is also low (20%-40%), and the relative molecular mass of the product is too large, usually only up to About 10,000, while the relative molecular mass of chitosan with higher physiological activity is below 5,000; second, because chitosan is insoluble in water, after dissolving in acid solution, the visco

Method used

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  • Preparation of crust oligosaccharide and use
  • Preparation of crust oligosaccharide and use

Examples

Experimental program
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Effect test

Example Embodiment

[0036] Example 1

[0037] Dissolve 30 kg of chitosan raw materials in 500L of 3% acetic acid and 2% hydrochloric acid solution, heat to 60-90°C, and keep stirring until chitosan is dissolved. Adjust the pH of the chitosan solution to 3.5, and dissolve 0.3 kg each of pectinase, pepsin, and lipase into the chitosan solution in a small amount of water, and stir and react at 40°C for 5 hours. The enzymatic hydrolysis reaction is complete. The enzyme is inactivated, filtered, and the filtrate is separated by ultrafiltration. The membrane molecule cut-off value is 5000. The ultrafiltrate is ion-exchanged with a strongly basic anion exchange resin type 717 with an ion exchange column at room temperature, so that the pH value of the effluent reaches 5.5-6.5. The ion exchange effluent is vacuum concentrated and dried to obtain the product chitosan powder.

Example Embodiment

[0038] Example 2

[0039] 50kg of chitosan raw material, when acid dissolving and heating pretreatment, first use 3% acetic acid solution to dissolve chitosan, at the same time add 4% hydrochloric acid solution, and heat to 90℃ to reduce the viscosity of chitosan solution, other conditions The same as in Example 1.

Example Embodiment

[0040] Example 3

[0041] Cellulase, papain and lipase are used as the composite enzyme composition, and the rest are the same as in Example 1.

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Abstract

The present invention relates to preparation and application of crust oligosaccharide and belongs to the field of deep chitin processing technology. Non-specific enzyme is used to raise the hydrolysis degree of chitosan and the separation and purification of hydrolytic product. The key point of the present invention is that the chitosan material is produced into crust oligosaccharide powder through acid dissolving, heating, enzymolysis and hydrolysis with compoiste, enzyme comprising polysaccharide hydrolase, proteinase and lipase, deactivating enzyme, filtering, ultrafiltering, ion exchange or nanometer filtering, vacuum concentration and drying. The product is used in producing various functional health foods with the functions of immunological regulation and blood fat regulation.

Description

technical field [0001] The invention relates to a preparation method and application of chitooligosaccharides, in particular to a preparation method of chitooligosaccharides using a compound enzyme hydrolysis method and the application of chitooligosaccharides, belonging to the technical field of chitin deep processing. Background technique [0002] Chitooligosaccharides are a class of water-soluble oligosaccharides linked by N-acetyl-D-glucosamine (NAG) and / or D-glucosamine (GA) through β-1,4 glycosidic bonds. Prepared by hydrolyzing chitin or chitosan. Chitin and chitosan can be obtained from the shells of shrimps and crabs, the shells of insects and the cell walls of fungi through acid-base treatment. This is a renewable resource, the total amount of resources on the earth is second only to cellulose. However, compared with cellulose, its structure contains acetamido or free amino groups in addition to hydroxyl groups. Its functional properties and uses are more importa...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 夏文水陈洁张家骊
Owner 上海华宝孔雀香精有限公司
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