254 results about "Glucosamine" patented technology

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The invention relates to the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides a cell for the production of a negatively charged, preferably sialylated, oligosaccharide wherein the cell is genetically engineered to have or express, preferably overexpress, a hydrolytic UDP-N-acetyl-D-glucosamine-2-epimerase. The invention further provides the use of said cells in culture or incubation. Also described are methods of producing negatively charged, preferably sialylated oligosaccharides using the cells, and purification of the negatively charged, preferably sialylated oligosaccharides.

The invention belongs to the field of preparation of soil conditioners, and particularly relates to a soil loosening and root promoting soil conditioner and a preparation method thereof. A polymer formed by vinyltriethoxysilane, polyacrylic acid and coumarin under the catalytic action of citric acid is mixed with porous powder obtained after crushing and nutrient organic matter, and freeze-drying is performed to obtain the soil-loosening improved fermentation substrate which can be slowly released in soil; polyaspartic acid and acetylchitosamine are cross-linked under the catalytic action of N-hydroxysuccinimide, root-promoting nutrition powder and auxiliary root-promoting components are mixed, and the obtained composite root-promoting agent can significantly promote the growth of plant root systems; a porous structure contained in the protective stability maintaining agent can provide a relatively stable microenvironment for effective components, and the soil loosening and root promoting agent is mixed with the soil loosening and improving fermentation substrate and the composite root promoting agent, so that the prepared soil loosening and root promoting soil conditioner is remarkable in soil loosening and root promoting effect, long-acting and stable, small in dosage, obvious in effect and stable in conditioning effect after being stored.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

The disclosure relates, in part, to the use of compositions of poly N-acetylated glucosamine (PNAG), deacetylated PNAG (dPNAG), and antibodies and antibody fragments specific to PNAG in the prevention and treatment of filariasis.

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The application relates to a cartilage organoid based on a DNA-silk fibroin hybrid hydrogel sustained-release system and a preparation method and application thereof. The DNA-silk fibroin hybrid hydrogel sustained-release system is a hydrogel system after a DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution is printed through a digital light processing system, wherein the DNA-silk fibroin hybrid hydrogel sustained-release system premixing solution contains DNA, silk fibroin, acrylated RGD peptide, acrylated polyethylene glycol NHS ester, glucosamine, TD-198946 and 2,4,6-trimethylbenzoyl lithium phosphate. Compared with the prior art, the glucosamine and TD-198946 introduced in the DNA-silk fibroin hybrid hydrogel sustained-release system can significantly improve the cartilage differentiation efficiency of bone marrow mesenchymal stem cells; and the application can be transplanted to a cartilage defect as a cartilage graft to promote cartilage regeneration and repair.

The present invention relates to a highly soluble composition containing cosmetic actives, its preparation and application, belonging to the technical field of cosmetics. The highly soluble composition containing cosmetic actives of the present invention comprises cosmetic actives and a solvent; the cosmetic actives are selected from one or more of glycyrrhetinic acid, asiatic acid and madecassic acid; the solvent comprises hexylene glycol, methyl glucamine and water. Methyl glucamine has multiple hydroxyl groups in its structure and has good water solubility. By combining the amino group in its structure with the carboxyl group on the cosmetic actives, the hydrophilicity of the cosmetic actives is increased. Hexylene glycol has the characteristics of being amphiphilic for oil and water and can play a bridging role in dissolving the cosmetic actives, promoting the combination of the cosmetic actives and methyl glucamine, thus playing a synergistic effect. At the same time, the amount of solvent used can be greatly reduced, and the dissolution efficiency of the cosmetic actives can be greatly improved, thereby realizing the application value in cosmetics.

The invention discloses a fluorescent probe aiming at D-glucosamine as well as a preparation method and a detection method thereof. The specific chemical structural formula of the fluorescent probe is as follows: # imgabs0 # fluorescent probe has the advantages of simplicity in preparation, high detection sensitivity and good selectivity.

The invention relates to the technical field of medicinal chemistry, and provides a TLR4 agonist, application thereof and a vaccine composition. The TLR4 agonist is a compound with a structure as shown in a formula 1 or a pharmaceutically acceptable salt thereof, the compound is glucosamine aminoalkyl 4-phosphate series molecules derived from MPL-12 structure modification, and the series molecules have enhanced immunostimulatory activity and selective immune response induction capability.

The invention relates to a gastric acid-resistant sustained-release intestinal bacteria capsule based on bionic mucous membrane-enzymolysis response and a preparation method thereof. The gastric acid-resistant sustained-release intestinal bacteria capsule comprises a core layer, an adhesive layer, an outer-layer composite film and a protective coating from inside to outside, the core layer consists of intestinal flora and a multifunctional freeze-drying protective agent; the adhesive layer is composed of trehalose, N-acetyl glucosamine and MUC2 recombinant protein; the outer-layer composite film is composed of a base material and a functional filler; the protective coating is composed of beewax and chitosan. The anti-gastric acid sustained-release intestinal bacteria capsule based on bionic mucosa-enzymolysis response provided by the invention has excellent gastric acid resistance, and the gastric acid resistance is remarkably improved; good slow release performance is achieved, and accurate slow release of enzymatic hydrolysis response can be achieved; the colonization efficiency of the intestinal bacteria can be effectively improved; the storage stability is good. The preparation method provided by the invention has the advantages of simplified steps, short time consumption, controllable core operation time and obviously shortened whole process, and is beneficial to keeping the activity of the flora.

The invention discloses a water quality conditioning agent based on plant extracts and a preparation method of the water quality conditioning agent, and belongs to the technical field of aquaculture. The water quality conditioning agent is prepared from the following raw materials in parts by weight: 14 to 18 parts of fructus schisandrae chinensis extract, 4 to 5 parts of fructus mume extract, 10 to 13 parts of rhizoma coptidis extract, 8 to 10 parts of glucosamine, 5 to 8 parts of chitosan, 3 to 6 parts of nitrifying bacteria, 2 to 4 parts of thiobacillus thiooxidans and 3 to 6 parts of zeolite powder. Through a four-dimensional synergistic mechanism of'plant active ingredient-chitosan slow release-microbial immobilization-zeolite adsorption ', the pH value, ammonia nitrogen, nitrite, hydrogen sulfide and bottom mud COD in tail water can be reduced and the dissolved oxygen amount can be increased under the condition of one-time addition, which indicates that the water quality regulator has a good effect of improving water quality.

The present invention relates to matrix-forming cross-linked carboxyalkyl chitosan, to compositions comprising same, to a process for the preparation thereof and to various uses thereof, in particular in therapeutic, rheumatology, ophthalmology, cosmetic medicine, plastic surgery, open surgery, dermatology, gynecology or cosmetic fields. In particular, the invention relates to a substrate comprising at least one carboxyalkyl chitosan having glucosamine units, N-acetylglucosamine units and glucosamine units substituted with a carboxyalkyl group, expressed in the number of moles of N-acetyl groups relative to the number of moles of total glucosamine units, the acetylation degree of the carboxyalkyl chitosan is 40%-80%, and the carboxyalkyl chitosan is crosslinked through covalent bonds between carboxyalkyl chitosan chains.

This invention belongs to the field of intestinal microbiome technology, specifically relating to a capsular polysaccharide derived from Bacteroides salorlis CSP6, its preparation method, and its applications. The monosaccharide composition of the capsular polysaccharide is mainly composed of fucose, glucose, and glucosamine, with a molecular weight of 7.139 × 10⁻⁶. 5 The strain described is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M20221993. In vitro studies have found that the capsular polysaccharide derived from Bacteroides Salieris CSP6 can inhibit α-glucosidase; in vivo animal experiments have demonstrated that this capsular polysaccharide can inhibit the rise in postprandial blood glucose and has a hypoglycemic effect.

The invention relates to the field of agricultural bactericides, in particular to a suspending agent containing fenaminstrobin and validamycin A and application of the suspending agent. The bactericidal composition comprises a suspending agent containing fenaminstrobin and validamycin A, an active component, a stabilizer, an auxiliary agent and water, the active component accounts for 1-70% of the mass of the preparation, and the stabilizer accounts for 0.1-10% of the mass of the preparation; wherein the active ingredients are fenaminstrobin and validamycin A, and the stabilizer is meglumine (N-methyl-D-glucosamine). The suspending agent prepared by the technical scheme of the invention effectively inhibits the increase of the particle size of a sample in the thermal storage process of a preparation, effectively improves the suspension rate of fenaminstrobin after the thermal storage of the preparation, improves the utilization rate of a pesticide, and ensures the normal exertion of the efficacy of the pesticide at the same time. Meanwhile, the quality of the active components of the stabilizer-containing bactericide is stable within the shelf life, the addition of the stabilizer does not cause the decomposition of the active components, and the preparation does not have obvious physical or chemical change.

The invention discloses a preparation method of a novel composite carbon source. The preparation method comprises the following steps: S1, preparing a solution; s2, preparing a culture medium; s3, extracting a genome; s4, extraction of plasmid DNA (deoxyribonucleic acid); s5, preparation of agarose gel electrophoresis; s6, constructing a linearized vector plasmid; s7, preparing an electrotransfection competent cell; and S8, electric conversion treatment. According to the preparation method of the novel composite carbon source, pfkA and pfkB can be respectively, independently and simultaneously knocked out when the carbon source is prepared by utilizing a gene editing technology, the consumption of a glucosamine precursor 6-fructose phosphate in an EMP pathway can be reduced, more glucose is used for synthesizing a target product, and by optimizing the preparation process of the composite carbon source, the yield of the target product is improved. The shake-flask fermentation yield of the glucosamine can be obviously improved, the conversion rate of the composite carbon source is also improved, and cell growth and synthesis of the target product glucosamine can be reasonably balanced, so that the yield of the glucosamine is improved.

To provide a compound and the use thereof for the treatment for regeneration of connective tissue following the degeneration process of articular cartilage due to osteoarthritis, in the case of humans, the degeneration process of articular cartilage caused by excessive stress, for the treatment of skin aging, and for the prevention of skin cancer.SOLUTION: For example, an amino acid derivative of glucosamine having the following structure is provided.SELECTED DRAWING: None

The invention relates to a plant vaccine lead compound based on carbohydrates and a synthesis method thereof, glucosamine groups are modified by an organic synthesis process, and a derivative with a tetrasaccharide structure, namely a compound 12, with induction activity is obtained by a glycosylation reaction. And then removing part of functional groups to obtain a chitosan oligosaccharide analogue with a clear structure and a single component, namely a compound 14. Through experimental research, optimization of a technology for inducing plant disease resistance by the chitosan oligosaccharide is realized. Results show that the plant vaccine lead compound provided by the invention can obviously promote the growth and development of plants at a lower concentration, so that the product provided by the invention has a good popularization and application prospect in the related field of agriculture.

The present invention discloses a relay aquaculture method for Penaeus japonicus, and relates to the field of biological aquaculture. The method employs a mixture of a protein source, a fat source, a complex vitamin, a complex inorganic salt, an attractant, and (-)-caryophyllene oxide-modified xanthan gum to prepare a bait for the relay aquaculture of Penaeus japonicus, resulting in a good survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus. The method also employs glucosamine-modified humic acid, which is used in the preparation of an amino acid fertilizer paste, which is then used in the relay aquaculture of Penaeus japonicus, resulting in an even better survival rate, specific growth rate, and feed conversion coefficient for the Penaeus japonicus.

A hydrogel for treating body cavity injury is derived from a chitosan derivative obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan by dehydration condensation. The hydrogel for treating the body cavity injury is granular, can be injected into a body cavity by utilizing an injector, and is used for treating the wound of the body cavity. A body cavity injury treatment kit is provided with a syringe, a tube connected to the syringe, and a granular body cavity injury treatment hydrogel filled into the syringe, the body cavity injury treatment hydrogel being derived from a chitosan derivative, the chitosan derivative is obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan through dehydration condensation.

A collagen-boosting supplement that contains at least 4, optionally at least 10, collagen building block amino acids and is substantially free of histidine. The building block amino acids may be selected from: Glycine, L-Proline, L-Hydroxyproline, L-Glutamic Acid, L-Alanine, L-Arginine, L-Aspartic Acid, L-Serine, L-Leucine, L-Lysine, L-Hydroxylysine, L-Valine, L-Phenylalanine, L-Threonine, L-Isoleucine, L-Methionine, and L-Tyrosine. The supplement may further comprise: vitamin C; one or more mast cell stabilisers which may be selected from: quercetin, molybdenum, bromelain, DAO (diamine oxidase), Urtica Dioica (stinging nettle), Nigella Sativa (black cumin), Zingiber (ginger), Scutellaria baicalensis (Chinese skullcap), Sambucus (Elderberry), Aloe Vera, Syzygium aromaticum (clove), and matcha; one or more collagen-strengthening herbs which may be selected from: Bilberry, Centella, and Astragulus; one or more Glucosaminoglycans (GAGs) which may be selected from: Hyaluronic acid, glucosamine, and chondroitin; one or more antioxidants which may be selected from: astaxanthin and resveratrol; and / or one or more cofactors which may be selected from: zinc, biotin, silicon, manganese, magnesium, Vitamin D, Vitamin A, Vitamin E, and iron. The supplement may be a foodstuff, medicament, nutraceutical, or cosmetic. The supplement may be used to treat hypermobile Ehlers-Danlos syndrome (hEDS) while reducing the likelihood of mast cell degranulation through histamine activation.

The utility model discloses a glucosamine particle grinding device, and relates to the technical field of grinding equipment, the glucosamine particle grinding device comprises a rack, the top of the rack is fixedly provided with a pedestal, and a grinding mechanism is arranged above the pedestal; the grinding mechanism comprises a lower shell which is fixedly installed at the upper end of the pedestal, and an upper shell is fixedly installed above the lower shell. In the grinding process, screening can be directly carried out in the equipment, ground particles do not need to be guided out and screened again through parts such as a filter screen, the operation process is greatly simplified, the machining efficiency is improved, the grinding mechanism adopts the semicircular cavity design, the grinding gap between the grinding roller and the filter plate is adjustable, and the grinding efficiency is improved. Efficient grinding can be carried out according to different particle sizes, the grinding quality is ensured, meanwhile, the filter plate is tightly attached to the lower shell, powder flying is effectively reduced, and the working environment is improved.

A modified hyaluronic acid derivative is described having the —CH2-OH group of the N-acetyl-D-glucosamine unit (GlcNAc) modified to an aldehyde group having the structure —CH2-O—CH2-CHO where the degree of modification is 1.0% to 15.0% for preparing a crosslinked hydrogel for use in aesthetic applications. The modified hyaluronic acid derivative is prepared by reacting a glycerol-modified hyaluronic acid with an oxidizing agent such as periodate.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The present invention relates to a method for producing α-sialyl-β-D-galactoside saccharides, particularly α-sialyl-(2→3)-β-D-galactoside saccharides and α-sialyl-(2→6)-β-D-galactoside saccharides, from a β-D-galactoside saccharide, a sialic acid donor, and an enzyme with β-galactoside α-sialyltransferase activity. The enzymes with β-galactoside α-sialyltransferase activity used herein do not exhibit catalytic activity towards the hydrolysis of cytidine 5'-monophospho-N-acetyl-neuraminic acid to cytidine and N-acetyl-neuraminic acid. The method can be performed in vitro and in vivo using a genetically engineered cell comprising a nucleic acid encoding said enzyme. Further, said process may be adapted to produce the sialic acid donor CMP-Neu5Ac from low-cost substrates N-acetyl-D-glucosamine (GlcNAc), pyruvate, a cytidine phosphate (CMP, CDP or CTP) and polyphosphate in a single reaction mixture with a set of optionally immobilized or optionally co-immobilized enzymes comprising N-acylglucoamine 2-epimerase (AGE), an N-acetylneuraminate lyase (NAL), an N-acylneuraminate cytidylyltransferase (CSS), optional a uridine kinase (UDK), a uridine monophosphate kinase and a polyphosphate kinase 3 (PPK3).

Provided are methods for producing polysaccharides in bacteria by expressing in a bacterium one or more coding sequences selected from the group consisting of a pglF dehydrogenase coding sequence, a wbpP UDP-N-acetyl-d-glucosamine C4 epimerase coding sequence, a wcfR aminotransferase coding sequence, and a wcfS phospho-glycosyltransferase coding sequence, a wcfQ glycosyltransferase coding sequence, a wcfO pyruvyltransferase coding sequence, a wcfP glycosyltransferase coding sequence, a wcfM UDP-galactopyranose mutase coding sequence, a wcfN glycosyltransferase coding sequence, a wza polysaccharide export protein coding sequence, a wzx fippase coding sequence, a wzy polymerase coding sequence, and a wzz coding sequence, wherein at least one of the coding sequences is heterologous to the bacterium. Also provided are expression cassettes with one or more of the disclosed coding sequences, recombinant bacteria that harbor one or more of the expression cassettes, and methods for producing immunogenic compositions using the polysaccharides produced by the recombinant bacteria.

To provide antimicrobial vaccine compounds.SOLUTION: Provided is antimicrobial vaccine compounds and compositions comprising oligosaccharide β-(1→6)-glucosamine groups having from 3 to 12 glucosamine units and linked through a linker group to tetanus toxoid.SELECTED DRAWING: Figure 1

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.