96 results about "Glucosamine" patented technology

The present invention relates to matrix-forming cross-linked carboxyalkyl chitosan, to compositions comprising same, to a process for the preparation thereof and to various uses thereof, in particular in therapeutic, rheumatology, ophthalmology, cosmetic medicine, plastic surgery, open surgery, dermatology, gynecology or cosmetic fields. In particular, the invention relates to a substrate comprising at least one carboxyalkyl chitosan having glucosamine units, N-acetylglucosamine units and glucosamine units substituted with a carboxyalkyl group, expressed in the number of moles of N-acetyl groups relative to the number of moles of total glucosamine units, the acetylation degree of the carboxyalkyl chitosan is 40%-80%, and the carboxyalkyl chitosan is crosslinked through covalent bonds between carboxyalkyl chitosan chains.

The invention relates to the field of agricultural bactericides, in particular to a suspending agent containing fenaminstrobin and validamycin A and application of the suspending agent. The bactericidal composition comprises a suspending agent containing fenaminstrobin and validamycin A, an active component, a stabilizer, an auxiliary agent and water, the active component accounts for 1-70% of the mass of the preparation, and the stabilizer accounts for 0.1-10% of the mass of the preparation; wherein the active ingredients are fenaminstrobin and validamycin A, and the stabilizer is meglumine (N-methyl-D-glucosamine). The suspending agent prepared by the technical scheme of the invention effectively inhibits the increase of the particle size of a sample in the thermal storage process of a preparation, effectively improves the suspension rate of fenaminstrobin after the thermal storage of the preparation, improves the utilization rate of a pesticide, and ensures the normal exertion of the efficacy of the pesticide at the same time. Meanwhile, the quality of the active components of the stabilizer-containing bactericide is stable within the shelf life, the addition of the stabilizer does not cause the decomposition of the active components, and the preparation does not have obvious physical or chemical change.

A hydrogel for treating body cavity injury is derived from a chitosan derivative obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan by dehydration condensation. The hydrogel for treating the body cavity injury is granular, can be injected into a body cavity by utilizing an injector, and is used for treating the wound of the body cavity. A body cavity injury treatment kit is provided with a syringe, a tube connected to the syringe, and a granular body cavity injury treatment hydrogel filled into the syringe, the body cavity injury treatment hydrogel being derived from a chitosan derivative, the chitosan derivative is obtained by bonding aldonic acid to an amino group of a glucosamine unit of chitosan through dehydration condensation.

A modified hyaluronic acid derivative is described having the —CH2-OH group of the N-acetyl-D-glucosamine unit (GlcNAc) modified to an aldehyde group having the structure —CH2-O—CH2-CHO where the degree of modification is 1.0% to 15.0% for preparing a crosslinked hydrogel for use in aesthetic applications. The modified hyaluronic acid derivative is prepared by reacting a glycerol-modified hyaluronic acid with an oxidizing agent such as periodate.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The present invention relates to a method for producing α-sialyl-β-D-galactoside saccharides, particularly α-sialyl-(2→3)-β-D-galactoside saccharides and α-sialyl-(2→6)-β-D-galactoside saccharides, from a β-D-galactoside saccharide, a sialic acid donor, and an enzyme with β-galactoside α-sialyltransferase activity. The enzymes with β-galactoside α-sialyltransferase activity used herein do not exhibit catalytic activity towards the hydrolysis of cytidine 5'-monophospho-N-acetyl-neuraminic acid to cytidine and N-acetyl-neuraminic acid. The method can be performed in vitro and in vivo using a genetically engineered cell comprising a nucleic acid encoding said enzyme. Further, said process may be adapted to produce the sialic acid donor CMP-Neu5Ac from low-cost substrates N-acetyl-D-glucosamine (GlcNAc), pyruvate, a cytidine phosphate (CMP, CDP or CTP) and polyphosphate in a single reaction mixture with a set of optionally immobilized or optionally co-immobilized enzymes comprising N-acylglucoamine 2-epimerase (AGE), an N-acetylneuraminate lyase (NAL), an N-acylneuraminate cytidylyltransferase (CSS), optional a uridine kinase (UDK), a uridine monophosphate kinase and a polyphosphate kinase 3 (PPK3).

The present invention discloses a glucosamine derivative nanoparticle and preparation method and use thereof. By self-assembling glucosamine derivatives and ethanol into the form of nanoparticles, the skin penetration rate and cell absorption rate of glucosamine are improved, and at the same time the toxicity to cells and organisms is reduced. The problem of poor absorption of glucosamine is improved by using glucosamine derivative nanoparticles. Furthermore, the glucosamine derivative nanoparticles can be used as a delivery carrier to cover and bring the specified ingredients into the cells or stratum corneum, and to increase the skin penetration rate and cell absorption rate of the specified ingredients.

The present invention is a disinfecting composition includes a glucosamine-based saccharide polymer, a carboxylic acid, an amino acid-based cationic surfactant, and a non-ionic surfactant. The disinfecting composition includes a carboxylic acid to glucosamine-based saccharide polymer ratio of at least about 1. A viscosity of the glucosamine-based saccharide polymer is less than about 50 cps. Upon drying, the disinfecting composition forms a substantially clear and non-tacky film.

The invention discloses sialylated-N-acetyl lactosamine and synthesis thereof, sialic acid (Neu5Ac) and N-acetyl lactosamine (LacNAc) or sialic acid (Neu5Ac), galactose (Gal), N-acetyl glucosamine (GlcNAc) and the like are used as raw materials, and the sialylated-N-acetyl lactosamine is synthesized through a one-step method. The method for catalytically synthesizing 6 '-SLN and 3'-SLN through a novel one-pot multi-enzyme catalytic system containing an NTP cyclic regeneration system is developed, the method is low in production cost and high in synthesis efficiency, and a foundation is laid for industrialization of sialic acid oligosaccharides such as 6 '-SLN and 3'-SLN.

The hydrochloric acid hydrolysis reaction device comprises a kettle body, a jacket is arranged at the kettle body, a heating pipe is arranged in the jacket, a steam inlet and a steam outlet are formed in the kettle body, a discharging opening is formed in the bottom of the kettle body, a motor is installed at the kettle body, and an output shaft of the motor is connected with a stirring rod. The kettle body is provided with a feeding hole, an acid adding interface, a vacuumizing interface and a tail gas interface, a feeding barrel which is positioned in the kettle body and is fixedly connected with the kettle body is mounted at the feeding hole, openings are formed in the top and the bottom of the feeding barrel, and a feeding cover unit is detachably mounted at the feeding hole. According to the reaction device, the vacuum pump connector is arranged, so that a vacuum pump is conveniently connected, the vacuum pump is opened while the feeding opening is opened, acid mist is prevented from overflowing from the feeding opening, and the damage of the acid mist to feeding personnel is prevented. A feeding cover unit is arranged at the feeding opening, and when the feeding cover unit is opened, a bottom baffle can seal the feeding barrel, so that acid mist is prevented from overflowing.

The invention relates to lentinan as well as a preparation method and application thereof, monosaccharide of the lentinan comprises glucose, galactose, mannose, fucose and glucosamine, and the molar ratio of the glucose to the galactose to the mannose to the fucose to the glucosamine is (85.0 to 88.0) to (5.5 to 6.5) to (3.5 to 4.1) to (1.8 to 2.2) to (1.0 to 1.2). According to the preparation method disclosed by the invention, a composite filler consisting of AB-8 macroporous resin and acidic aluminum oxide is adopted, static adsorption decoloration is carried out when the optimal mass ratio is 2: 3, an extraction process of combining graded hot water extraction with negative pressure concentration is optimized, and on the premise of avoiding using an organic solvent, efficient purification of lentinan is realized, so that the retention rate of the lentinan reaches 22.38%, and the yield of the lentinan is improved. And the beta-triple helix active structure can be well reserved.

The present disclosure provides methods and kits for detecting Group A Streptococcus in biological samples. More particularly, the present disclosure provides methods for enhancing the specificity and sensitivity of Group A Streptococcus immunoassays by including N-propionyl-D-glucosamine, 2-N-butanoyl-D-glucosamide, Bis-(2-(D-2-deoxy-glucosaminyl))-PEG3-amide, m-PEG4-glucosamine, m-PEG6-glucosamine, or m-PEG10-glucosamine. The methods and kits disclosed herein are thus useful for reliable and early diagnosis of streptococcal infections in a subject.

1.The name of the design product: packaging bottle (glucosamine orange-flavored effervescent tablet). 2.The use of the design product: the design product is used for packaging. 3.The design points of the design product: the combination of shape, pattern and color. 4.The picture or photo that best indicates the design points: front view. 5.The design claimed contains color.

This application relates to the technical field of vaccine quality testing, specifically to a quantitative detection method for 3D-MLA in liposome adjuvants. The method includes the following steps: taking a test sample solution, adding hydrochloric acid for acid hydrolysis to completely hydrolyze 3D-MLA into D-glucosamine and D-glucosamine-6-phosphate; adding an internal standard solution and a derivatizing reagent to the hydrolyzed sample to perform a derivatization reaction, converting the glycosamine and internal standard into fluorescent derivatives; detecting the derivatized sample using high-performance liquid chromatography-fluorescence detector; and calculating the 3D-MLA content in the test sample using a standard curve method based on the ratio of the total peak area of ​​glucosamine to the peak area of ​​the internal standard. The detection method provided in this application is highly specific, sensitive, easy to operate, and suitable for large batches of samples.

The invention belongs to the field of gene engineering, and particularly relates to a construction method of a gene engineering strain for producing glucosamine, a product and application. According to the method, intestinal probiotics with colonization ability are taken as an original strain, and multi-round CRISPR-Cas9 and Red homologous recombination technology combined transformation is carried out: on the basis of knockout of manX, ptsG is knocked out, a gna1 gene cluster derived from saccharomyces cerevisiae is inserted, then nagE is knocked out, and glmS driven by a strong promoter is inserted. In cell co-culture, the strain can safely and effectively promote phenotype transformation of a murine microglial cell line from BV2 to M2. In an animal model, the strain can colonize intestinal tracts in a short time and produce glucosamine, the effective concentration duration of glucosamine in serum is remarkably prolonged, inflammation is continuously inhibited, and the effect of enhancing immunity is achieved. And the strain does not have obvious toxicity, so that a new way is opened up for treating inflammatory diseases of engineering probiotics.

This invention discloses a highly hydrophilic organic solvent reverse osmosis membrane, its preparation method, and its applications. The organic solvent reverse osmosis membrane comprises a cross-linked polyimide porous support layer, a polyamide separation layer, and a surface grafting layer. The surface grafting layer is formed by the amidation reaction of D-glucosamine with residual acyl chloride groups on the surface of the polyamide separation layer via its primary amino group. This invention utilizes a D-glucosamine / ethanol solution to modify the surface of the nascent polyamide membrane, grafting D-glucosamine through a secondary interfacial reaction to avoid hydrolysis of residual acyl chloride groups, while simultaneously introducing a large number of hydroxyl groups onto the membrane surface. The prepared membrane exhibits significantly enhanced hydrophilicity, greatly increasing the permeation flux of polar organic solvents while maintaining a high solute rejection rate. The preparation method of this invention is simple, low-cost, and easy to scale up, showing broad application prospects in the field of polar organic solvent separation and recovery.

The invention belongs to the technical field of medicines, and particularly relates to forsythiaside glucosamine, a preparation method thereof and application of the forsythiaside glucosamine in treatment of osteoarthritis. The invention relates to design and synthesis of forsythiaside glucosamine, and researches on the treatment effect of forsythiaside glucosamine on an osteoarthritis mouse model. In the structural general formula of the compound, R1 is an amino group, a phthaloyl amino group or an allyloxy amido group, R2, R3 and R4 groups represent a hydroxyl group or an alkanoyloxy group, and the configurations of different head positions are alpha and beta. Wherein the alkanoyloxy group is a C1-C6 alkanoyloxy group or a deuterated alkanoyloxy group. The synthesized forsythiaside glucosamine is used as a twin drug, and shows better cartilage generation promoting activity and anti-inflammatory activity compared with combined use of forsythiaside and glucosamine on an osteoarthritis mouse model. In addition, under the same administration dosage, the activity of promoting cartilage generation and the like is obviously superior to that of forsythiaside acetylglucosamine in the prior art.

The present invention relates to novel Poly(D-glucosamine) co-crystals of Saroglitazar. Specifically, the present invention provides co-crystal of Saroglitazar of formula (I) and Chitosan, process for the preparation of said co-crystal, use thereof and pharmaceutical compositions comprising the same.

Provided is a method for purifying an organic solvent and a device for purifying an organic solvent, with which it is possible to reduce the amount of impurities such as metal impurities of as many kinds as possible from the organic solvent. The method for purifying an organic solvent includes an anion exchanger contact step for bringing an organic solvent to be purified into contact with an anion exchanger A and an anion exchanger B, wherein: the anion exchanger A is an anion exchanger having at least one ionic form that is selected from the group consisting of an OH form, a free base form, a carbonate form, and a bicarbonate form; and the anion exchanger B is a chelate resin that contains a glucamine-type ion exchange group.

The invention belongs to the technical field of marine polysaccharides, and relates to an anti-hypoxia urechis unicinctus body cavity fluid sulfated polysaccharide as well as a preparation method and application thereof. The weight-average molecular weight of the polysaccharide is 1000-50000 Da, the mass fraction of sulfate radicals is 10.0%-30.0%, and monosaccharide is prepared from galactose, ribose, fucose, glucosamine, mannose and glucose. The preparation method comprises the following steps: inducing controlled degradation of a main chain through high-temperature hot water extraction, carrying out proteolysis deproteinization, carrying out graded ethanol precipitation interception and carrying out anion exchange chromatography purification. The polysaccharide shows a remarkable protective effect in a nerve cell hypoxia simulation model, has excellent free radical scavenging activity, and can be used for preparing medicines, antioxidants, functional foods and the like for preventing or treating hypoxia-related diseases.

The invention provides a micro-fat calcium supplement carrier, which is prepared from the following raw materials in parts by weight: 30 to 35 parts of standard index calcium, 1 to 5 parts of chondroitin, 1 to 5 parts of glucosamine, 8 to 12 parts of pure camel milk, 0.7 part of vitamin D, 2 to 70.3 parts of vitamin K, 1 to 3 parts of phospholipid complex and 8 to 10 parts of excipient. According to the invention, standard calcium, chondroitin, glucosamine, pure camel milk, vitamin D, vitamin K2-7, a phospholipid complex and an excipient are organically mixed by adopting a liposome carrier technology, so that the digestion and absorption of calcium in a body and the distribution of calcium in a specific region are effectively improved, and further the effects of rapid bone growth, increase of bone length (promotion of height increase) and increase of bone density are achieved; the health-care food has the health-care functions of reducing the fracture probability, preventing tooth decay, reducing the death rate of cardiovascular diseases and the like, so that the calcium absorption rate is remarkably improved and reaches up to 95%.

This invention discloses a coltsfoot flower extract, its preparation method, and its uses, belonging to the field of pharmaceutical technology. The coltsfoot flower extract is a coltsfoot flower polysaccharide, comprising fructose, glucose, arabinose, galactose, and glucosamine, with a molecular weight of 1.21 × 10⁻⁶. 2 -6.58×10 6 Da; The deproteinized polysaccharide obtained from coltsfoot flower extract contains fructose, arabinose, glucose, and galactose, with a molecular weight of 4.86 × 10⁻⁶. 3 -4.34×10 6 Da; The decolorized polysaccharide obtained from coltsfoot flower extract contains fructose, arabinose, glucose, and galactose, with a molecular weight of 1.42 × 10⁻⁶. 2 -1.05×10 7 The above-mentioned polysaccharides have good effects in preventing and treating asthma, rhinitis, cough, and chronic obstructive pulmonary disease.

The embodiment of the application discloses an acid-responsive oxygen-releasing chitosan / polycaprolactone core-shell microsphere and a preparation method and application thereof. The microsphere comprises an oxygen-releasing inner core taking polycaprolactone and calcium peroxide as base materials, and a pH-responsive outer shell modified by chitosan through N-acetyl-d-glucosamine and loaded with a natural medicine 6-Gin; the microsphere is disintegrated in the outer shell under the gastric acid environment and rapidly releases 6-Gin, and the inner core continuously releases oxygen by reacting with water. The microsphere provided by the application can effectively inhibit bacterial adhesion, destroy a biofilm and directly kill H. pylori, and solves the problem of poor curative effect caused by a microenvironment in traditional therapy, and provides a new effective strategy for anti-H. pylori therapy.

The present invention relates to crosslinked carboxyalkyl chitosan forming a matrix, to compositions comprising the same, to methods for the preparation thereof and to various applications thereof, in particular in the therapeutic, rheumatological, ophthalmic, cosmetic, plastic surgery, open surgery, dermatological, gynecological or aesthetic fields. The present invention in particular relates to a matrix comprising at least one carboxyalkyl chitosan having glucosamine units, N-acetylglucosamine units and glucosamine units substituted with carboxyalkyl groups, the degree of acetylation of said carboxyalkyl chitosan being comprised between 40% and 80%, expressed in number of moles of N-acetyl groups relative to the number of moles of total glucosamine units, said carboxyalkyl chitosan being crosslinked by covalent bonds between carboxyalkyl chitosan chains.

This invention belongs to the field of biomedicine or food technology, specifically relating to a compound wine with anti-tumor effects. The main raw materials of this composition are N-acetyl-D-glucosamine and taurine, wherein the weight ratio of N-acetyl-D-glucosamine to taurine is 19.5-20.5:2.9-3.1. Studies have shown that when the proportion of taurine in the composition exceeds a certain level, the stability of N-acetyl-D-glucosamine decreases, while when the proportion of taurine in the composition is reduced to a certain level, the two do not have a synergistic effect in the anti-tumor model.

Disclosed are methods for the fermentative production of a sialylated saccharide and genetically engineered microbial cells for use in said method, wherein the genetically engineered microbial cells comprise (i) a sialic acid biosynthesis pathway comprising a glucosamine-6-phosphate N-acetyltransferase, (ii) a cytidine 5′-monophospho-(CMP)-N-acetylneuraminic acid synthetase; and (iii) a sialyitransferase, for producing sialylated saccharides, as well as the use of said sialylated oligosaccharides for providing nutritional compositions.

Provided are a hyaluronic acid derivative and a hyaluronic acid derivative cross-linked gel which have excellent in vivo swelling inhibition performance after gelling, and a pharmaceutical composition using the hyaluronic acid derivative and the hyaluronic acid derivative cross-linked gel. The present invention relates to: a hyaluronic acid derivative in which a steroid group is introduced to at least some of the carboxyl groups in the glucuronic acid moiety of hyaluronic acid, and a maleimide group is introduced to at least some of the hydroxyl groups in the N-acetylglucosamine moiety or the carboxyl groups in the glucuronic acid moiety of hyaluronic acid; and a hyaluronic acid derivative cross-linked gel which is a gel-like substance in which the hyaluronic acid derivative is chemically cross-linked by a cross-linking agent having two or more cross-linkable groups.

This invention relates to the field of glucose preparation technology and relates to an unsaturated glucosamine with retained amino groups, its preparation method, and its applications. It solves the problems of low polymer drug loading, severe drug burst release, and poor biocompatibility caused by the blocking of amino groups in existing polymerizable monomers of glucosamine, as well as the cumbersome, low-yield, and poor-purity problems of existing methods for retaining amino groups. The preparation method includes: adding acetyl chloride to 3-buten-1-ol, stirring evenly at 0-5°C, then adding D-glucosamine hydrochloride in batches, heating to 60-80°C, and reacting for 2-4 hours. After the reaction is complete, post-treatment is performed to obtain an intermediate compound; dissolving the intermediate compound in deionized water, adding barium hydroxide hydrate, heating to 90-110°C, and stirring for 12-20 hours, after which post-treatment is performed to obtain unsaturated glucosamine with retained free amino groups. This invention can be applied to the preparation of drug carriers and has broad application prospects in the biomedical field.

The invention provides a method for determining the content of sodium hyaluronate. The method comprises the following steps: (1) preparing D-glucosamine hydrochloride standard curve solutions with series concentrations; (2) preparing a sample solution; (3) respectively taking the standard curve solution and the sample solution, adding a PMP-methanol solution and a sodium hydroxide solution, reacting at a constant temperature, extracting, separating and filtering to respectively obtain a derivatization standard solution and a derivatization sample solution; (4) performing gradient elution on the derivatization solution to obtain data of a corresponding relation between the concentration and the peak area of the derivatization standard solution and data of the peak area of a target peak of the glucosamine-PMP derivative in the derivatization sample solution; and (5) calculating the content of sodium hyaluronate according to the standard curve. PMP derivatization enables glucosamine to be converted into a strong absorption derivative, and gradient elution realizes efficient separation. And the effects of small impurity interference in the complex matrix and high accuracy of low-content sample determination are realized.