115 results about "Boar semen" patented technology

The invention discloses a health-care full-price feed for improving semen quality and sexual desire of a breeding boar. The health-care full-price feed comprises the following components in parts by weight: 43-74 parts of corns, 5-20 parts of wheat brans, 10-30 parts of bean pulps, 2-5 parts of fish powder, 2-4 parts of corn protein powder, 1-5 parts of corn germ meal, 0.2-0.5 part of lysine hydrochloride (98.5 percent), 0.2-0.4 part of salt, 0.2 part of a plurality of vitamins, 0.2 part of a plurality of mineral substances, 0.3-0.5 part of calcium hydrophosphate, 0.6-0.9 part of mountain flour, and 0.6 part of Chinese herbal medicine powder. The Chinese herbal medicine has the functions of warming the kidney yang, nourishing the kidney yin, benefiting semen blood, strengthening the bones and muscles, improving a sex function, adjusting immunization, promoting growth of osteoblast, resisting stress, resisting oxidization, delaying senescence and facilitating hematopoiesis, and can improve the semen quality of the breeding boar and increases the sexual desire of the breeding boar.

The invention discloses an antifreeze used for freezing and preserving boar semens as well as preparation and application thereof. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, contains pigeon egg yolk and concretely comprises the following components in parts by volume: 82 to 90 of freezing base liquid and 10 to 18 of pigeon egg yolk. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, has simple preparation method, easy material acquirement, reliable effect and easy popularization, is suitable for insemination after long-time preservation and long-distance transportation after the artificial insemination of the frozen semens of the tubules of boars and the ultra-low temperature freezing of the semens of the boars, can obviously enhance the survival rate of the semens, the integrity of the acrosomes and the integrity of the plasmalemma after the boar semens are frozen and unfrozen, and keeps the insemination activity of the boar semens.

The invention discloses a boar semen refrigerating method and dedicated refrigerating fluid thereof. The method comprises the following steps: (1) diluting dense boar semen according to a volume ratio of 1:(0.5-3), stewing at 26-30 DEG C for 25-40 minutes, and centrifuging 800g of boar semen for 10 minutes to obtain sperm precipitation; (2) adding refrigerating fluid I into the sperm precipitation according to a volume ratio of 1: (1-1.5), stewing at 17-20 DEG C for 1-2 hours, and stewing at 4-7 DEG C for 2 hours to obtain a system I; (3) adding refrigerating fluid II containing glycerin into the system I to obtain a system II with final glycerin concentration of 3%; (4) stewing the system II at 4 DEG C for 1-2 hours, and adding caffeine to obtain a system III with final caffeine concentration of 0.2g / L; and (5) stewing the system III at 4 DEG C for 0.5-2 hours, dropwise refrigerating at -120 DEG C, stewing and balancing for 5-10 minutes, and submerging the system III in liquid nitrogen for cryopreservation. After the boar semen refrigerated by the method is thawed, the sperm survival rate of the boar semen is higher than 0.45, and the in vitro survival time of the boar semen is longer than 6 hours, thereby reaching the production application level. The boar semen refrigerating method disclosed by the invention is used for providing scientific basis for the application and popularization of the refrigerated boar semen in production, and providing a technical method and standard for boar semen refrigerating.

The invention relates to an antifreeze agent for diluting pig sperm, pertaining to the technical field of animal breeding. The antifreeze agent is prepared by N-acetyl-D-dextrosamine, hyaluronic acid, amido-sodium-lauryl sulfuric ester, glucose, trihydroxymethyl aminomethane, sodium citrate, sodium bicarbonate, potassium chloride, bactericide, distilled water and the like, wherein, the N-acetyl-D-dextrosamine can stimulate the emulsification function of the amido-sodium-lauryl sulfuric ester, enhances the protection function of the amido-sodium-lauryl sulfuric ester and yolk, the hyaluronic acid and the amido-sodium-lauryl sulfuric ester can effectively inhibit the damage rate of freezing and thawing sperm plasma membrane and DNA and improve the quality of freezing and thawing sperm, the glucose is the energy source for metabolism in vitro of the sperm, and the sodium citrate can maintain the osmotic pressure balance between diluted antifreeze agent environment and the internal environment of the sperm. The antifreeze agent for diluting pig sperm can effectively prolong the survival time of pig sperms for ultra-low temperature cryopreservation, and improve the liveliness of the freezing and thawing pig sperms, the integrity of plasma membrane and the integrity of acrosome.

The invention discloses a special concentrated feed for improving the quality of boar semen, which comprises the following components in parts by weight: 200-300 parts of corn flour, 300-360 parts of low-antigen soybean meal, W3 fatty acid, 60-100 parts of mountain flour, 80-100 parts of calcium hydrophosphate, 30-50 parts of salt, 1-5 parts of complex vitamins, 20-25 parts of complex minerals, 1-5 parts of VC, 1-5 parts of VE, 1-5 parts of yeast selenium, 1-5 parts of sweetener, 1-5 parts of antioxidant, 1-5 parts of flavoring agent and 1-10 parts of complex enzyme preparation, wherein the concentration of the yeast selenium is 0.1%. The special feed of the invention can improve the quality of the boar semen, improve the conception rate of sows and reduce the breeding return rate, thereby reducing the nonproductive number of days of the sows and improving the litter size and the total number born of each sow per year.

The invention relates to a method for preparing boar semen dilution powder, comprising the following steps: taking 45-55g of dextrose powder, 2.5-3.5g of sodium citrate dehydrate, 0.8-1.2g of sodium ethylene diamine tetracetate, 500-800 thousand units of penethamate, 500-1000 thousand units of dihydrostreptomycin and 2.5-3g of egg yolk dry powder; and evenly mixing the dextrose powder, the egg yolk dry powder, the sodium citrate dehydrate, the sodium ethylene diamine tetracetate, the penethamate and the dihydrostreptomycin to obtain boar semen dilution powder. The method for preparing the egg yolk dry powder comprises the following steps: cooking fresh eggs, taking out yelk and drying the yelk into egg yolk dry powder with a water content of 7-10percent. In the invention, the egg yolk dry powder has rich phosphatidylcholine to ensure the normal metabolism of the semen and has the functions of nutrition and semen shock prevention after a lower limit period at a preserving temperature. The boar semen diluted by adopting the invention has a preserving period not less than five days, ensures a fertility rate above 95percnet and a birth number of 11.6.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.

The invention discloses a feed for improving the quality of boar semen. In the feed, organic trace elements and vitamins which are matched with one another are added to satisfy the needs of boar mating; and an exogenously synthesized amino acid is provided and a balanced amino acid mode is adopted to make main restrained amino acid of daily ration tend to a balanced state. The feed aims to increase feed intake of boars, improve the quality of boar semen and boar mating ability and prolong the life of the boars.

The present invention discloses a swine gender-controlling sperm and preparation method and application thereof, which comprises diluting the original boar semen with the original semen diluent containing seminal plasma, genistein and cow urine phenol, separating the diluted original boar semen by the flow cytometry and recycling the obtained swine gender-controlling sperm into the freezing diluent containing seminal plasma, genistein and cow urine phenol. The method provided by the present invention has simple operation, which can effectively prevent the separation and the cryopreservation of pig X, Y sperm from damaging, thereby improving the sperm quality and the fertilization ability, and prolonging the survival time of sperm, wherein the separated sperm can be used in low dose insemination, so as to reduce the production cost. The method can be widely applied to commercial promotion with broad market prospects.

The invention provides a preparation method of long-acting boar semen diluting powder. According to the method, 26-36 g of glucose, 3-7 g of dry chicken egg-yolk powder, 3-6 g of sodium citrate, 1.5-2.5 g of aminoacetic acid, 1.0-2.0 g of disodium hydrogen phosphate, 1.0-2.0 g of ethylene diamine tetraacetic acid disodium, 2.0-4.0 g of potassium sorbate, 1.0-2.0 g of calcium propionate, and 0.005-0.02 g of gentamicin powder are taken; the powdery raw materials taken according to the previous quantities are evenly mixed together so that the boar semen diluting powder is obtained. The preparation method of the dry chicken egg-yolk powder comprises the following steps: cooking a fresh chicken egg, taking out the egg yolk and drying the egg yolk to obtain dry chicken egg-yolk powder with the moisture content of 1-10%. The nutrient solution for diluting boar semen prepared by the raw materials provided in the method and distilled water contains a nutritional agent, a diluting agent, a buffering agent, a pH regulator and an antibiotic, as well as a preservative and a bacteriostatic agent, and can be preserved for 0-12 days under the condition of a constant temperature ranging from 15 to 20 DEG C.

The invention relates to a traditional Chinese medicine compound boar feed capable of improving the quality of boar semen. The traditional Chinese medicine compound boar feed comprises a feed main material and a traditional Chinese medicine additive, wherein the feed main material comprises dried corns, 43% soybean meal, 46% soybean meal, bran, DDGS, a liquid enzyme preparation and a boar feed premix; and the traditional Chinese medicine additive comprises cortex acanthopanacis radicis, cortex eucommiae, herba epimedii, semen cuscutae and radix rosae laevigatae. The traditional Chinese medicine compound boar feed provided by the invention can be prepared according to a reasonable and innovative formula and is low in cost, and the quality of the boar semen can be improved.

The invention discloses a freezing and unfreezing method for boar semen. The method includes the steps of: (1) collecting excellent breeding boar semen, conducting semen detection, then carrying out dilution in proportion, and then performing balancing for 0.5-1h; (2) transferring the semen into a 15DEG C condition, and carrying out cooling balancing for 3h; (3) conducting centrifugation under a 15DEG C condition (800g, 10min), adding a glycerol-free I solution, slowly lowering the temperature to 5DEG C, performing balancing for 2h, then adding a glycerol-containing II solution, and then conducting subpackaging; (4) carrying out the freezing procedure, then putting the product into liquid nitrogen for preservation; (5) carrying out unfreezing on the contrary to the freezing principle; and (6) detecting the quality of the unfrozen semen. With the method provided by the invention, the semen can be preserved for a long time, the utilization rate of boars is effectively improved, the cost of living body breed conservation is reduced, excellent gene drift and infectious diseases can be prevented, thus facilitating in-depth development of pig joint breeding so as to achieve greater genetic progress. The method provided by the invention has important research value and wide application prospects in protection of local excellent varieties, acceleration of breed improvement and other aspects.

Identification of a pig as resistant or non-resistant to enterotoxigenic E. Coli (EIEC). Particularly, there is provided methods, probes and DNA molecules involved in identifying a pig as resistant or non-resistant to ETEC. There is also provided methods for breeding pigs using the information of resistance / non-resistance, mixed boar semen, and methods for developing drugs to compensate for non-resistance to ETEC.

The invention provides a boar semen collection table and a semen collection method thereof. The boar semen collection table comprises a boar body, a bottom plate, a sound effect device, a rotary propand a lifting support column. Support legs are installed on the lower part of the boar body, the sound effect device is installed inside the boar body, the boar body is installed on the bottom plate through the rotary prop and the lifting support column, a soft wrap layer and a simulation layer are arranged on the outer wall of the boar body in sequence, and the outer wall of the front end of theboar body is sleeved with a soft wrap layer. Through simulation on the appearance of the semen collection table, a boar gets satisfied visually, through the induction of sound made by a sow in estrus,the boar induction time is shortened, the pleasant sensation of the boar in semen collection is improved, and the semen quality and quantity of the boar are improved; workers can collect semen conveniently through a lifting support.

The invention provides glutathione-contained boar semen cryopreservation liquid and a cryopreservation method thereof. The boar semen cryopreservation liquid uses diluent which is generally suitable for boar semen cryopreservation as a substrate and also comprises 2-6 mg / ml of glutathione, 5-11 mg / ml of lactose, 10-30 percent (v / v) of yelk, 5-10 percent (v / v) of glycerin and 0.5-2 mg / ml of sodium lauryl sulfate. The invention applies the glutathione to the boar semen cryopreservation, can effectively remove redundant free radicals generated in a refrigeration process, solves the problem of low survival rate of the prior boar semen cryopreservation, has lower economic cost and is convenient to popularize and apply in production.

The present invention relates to a porcine semen freezing and storing tube and a porcine semen freezing and storing method thereof. The porcine semen freezing and storing tube comprises a storing tube and is characterized in that the storing tube is a double-layer tube; the two vessel openings of the double-layer tube are opposite. The key points of the porcine semen freezing and storing method are that the frozen and diluted porcine semen after the pretreatment and the cooling treatment is cased into the annular vessel of the porcine semen freezing and storing tube of the present invention; then the porcine semen freezing and storing tube is inserted at liquid nitrogen to be suffocated for a plurality of minutes and directly put into the liquid nitrogen for long time storage, which can increase the contacting area of the semen and the liquid nitrogen during the process of freezing, accelerate semen freezing velocity, reduce the damage to the semen caused by freezing and improve the live-rate of frozen semen. The present invention also has the advantages of low cost, convenient operation, high efficiency, ensuring fecundation rate, etc.

The present invention relates to the technical field of culture and particularly discloses a simple method for comprehensively evaluating the quality of boar semen. The method comprises the following steps of: firstly, detecting four indexes, namely sperm density, semen quantity, sperm viability and malformation rate; secondly, calculating the total number of active sperms according to a calculation formula; and thirdly, evaluating the quality of the boar semen according to the total number of the active sperms. According to the method, the quality of the boar semen can be objectively and accurately evaluated.

A method for preparing an antioxidant diluent for low-temperature storage of pig seminal fluid is disclosed. The method includes preparing a yeast cell liquid, crushing yeast cells, adding an organic solvent, performing extraction to obtain a supernatant liquid, concentrating the supernatant liquid, and finally adding an auxiliary material into the concentrate. Yeast is extracted from waste beer's yeast mud and crushed, a yeast extract liquid is adopted as a base solution, and other active components are added to prepare the diluent. The power of microwaves and the concentration of added ethanol are controlled. A certain amount of yeast trehalose is extracted, a certain amount of procyanidin is successfully extracted from added grape powder, and the yeast trehalose and the procyanidin are proved to be good antioxidant compounds in the frozen-semen diluent, and can effectively improve pig seminal fluid storage effects of the diluent.

The invention discloses a traditional Chinese medicine feed additive for breeding boars and a preparation method thereof. The feed additive is prepared by the following steps: weighing epimedium, cistanche, morinda officinalis, okra, codonopsis pilosula, astragalus membranaceus, angelica, common yam rhizome and liquorice in parts by weight; adding a 70% ethanol solution; boiling with high flame and heating with slow fire for 30-60 minutes; cooling and filtering; collecting filter liquor; and concentrating the filter liquor to dryness to obtain the traditional Chinese medicine extract, wherein 1%-3% of traditional Chinese medicine extract is added into a daily ration of the breeding boars. The feed additive can be used for improving the immunity of a boar body and disease resistance of the boars and has a relatively good effect of improving the semen quality and the sexual desire of the breeding boars. The feed additive is easy to prepare, convenient to use, environmentally friendly, safe, and free of toxic and side effects and residues; the preparation method is suitable for industrialized production.

The invention discloses a feed additive and a mixed batch for improving the semen quality of breeding boars. The feed additive disclosed by the invention contains the following components in part by weight: 1-2 parts of broken-walled haematococcus pluvialis powder, 2.5-5 parts of small peptide proteins and 2-4 parts of organic zinc. The feed additive is adopted for feeding adult breeding boars, the sexuality of the breeding boars can be improved, and especially the semen quality can be increased in summer, for example, the semen production quantity, the semen vitality and the semen density are improved, the semen deformity rate is reduced, and the like.

The invention discloses a diluent for in-vitro low temperature preservation of boar semen and a preparation method thereof. The diluent comprises following components: broad-spectrum antibiotic bacteriostatic agents of D-fructose, 2-amino-2-hydroxymethyl-1,3-propanediol, trisodium citrate, sodium bicarbonate, citric acid, disodium ethylene diamine tetraacetate, N-acetyl-cysteine, skim milk powder, yolk freeze-dried powder, amikacini sulfatis and the like. The diluent for the in-vitro low temperature preservation of the boar semen is suitable for preserving the fresh boar semen at low temperature (4-5 DEG C). The diluent significantly inhibits injury caused to sperm structure during the low temperature preservation process, maintains glucose metabolism and oxidative phosphorylation metabolism processes of boar sperm, effectively prolongs survival time of the boar sperm in the low temperature preservation, and significantly improves use efficiency of the high quality breeding boar semen and economic benefit of breeding.

The invention discloses freezing and unfreezing methods of boar semen. The methods comprise a boar semen freezing method, a boar semen unfreezing method and a boar semen detecting method. The boar semen freezing method comprises the following steps: collecting breeding boar semen, determining the density of the breeding boar semen, and diluting the breeding boar semen; allowing the diluted semen to stand, and carrying out cooling balancing; centrifuging the cooled semen at 14-15 DEG C; sequentially adding a dilution solution I and a dilution solution II, and packaging the obtained semen; and freezing the packaged semen, and carrying out freeze preservation by using liquid nitrogen. The unfreezing method comprises the following steps: rapidly taking the frozen boar semen, heating the frozen boar semen to 5 DEG C from -140 DEG C at a rate of 50 DEG C / min, and heating the heated boar semen to 5 DEG C from -5 DEG C according to a rate of 3 DEG C / min in order to carrying out unfreezing. The invention provides a technology for promotion of the application of the frozen boar semen to production.

The invention belongs to the technical field of livestock artificial insemination, and specifically, relates to a boar semen room-temperature preservation dilution powder and diluent, and an application of the dilution powder and the diluent. The boar semen room-temperature preservation dilution powder comprises the components in parts by weight: 28-34 parts by weight of glucose, 1.5-2.5 parts by weight of sodium bicarbonate, 6.5-8.5 parts by weight of sodium citrate, 1.8-2.4 parts by weight of EDTA, 2.0-3.5 parts by weight of citric acid, 4.0-6.0 parts by weight of Tris, 3.0-5.0 parts by weight of Hepes, 1.0-2.5 parts by weight of PVP, 2.0-4.0 parts by weight of BSA, 0.05-0.10 part by weight of cysteine, and an antibiotic. The invention provides the brand-new boar semen room-temperature preservation dilution powder and diluent; an agglomeration phenomenon in the semen preservation process can be effectively reduced, the semen preservation time is prolonged, and the utilization efficiency of excellent breeding boars is expanded. Boar semen is diluted and subpacked by the whole-component diluent prepared by the formula and is preserved at the temperature of 17 DEG C, 3 days later, the semen vigor is 0.6 or more, and the pig breeding and production needs can be met.

The invention relates to nutrient premix improving semen quality of service boars. The nutrient premix comprises L-carnitine, organic chromium, N- carbamylglutamate, vitamin E and carotene, and defatted rice bran is used as a carrier. The nutrient premix is reasonable and innovative in formula and conforms to growth, development and physiological characteristics of boars; after application, semen quality of the boars can be remarkably improved, ejaculation of the boars is increased, sperm mobility is improved, sperm density is increased, and teratospermia rate is decreased. In addition, the nutrient premix is low in cost and complies with actual production of a pig farm.

The invention discloses trace element pre-mixed feed for a breeding boar. The trace element pre-mixed feed comprises glycine-iron complexes, copper sulfate, zinc methionine complexes, manganese methionine complexes, calcium iodate, yeast selenium, cobalt chloride, yeast chromium, antioxidants and medical stone. The trace element pre-mixed feed for the breeding boar can fully meet a special trace element nutrition requirement of the breeding boar, improves the health level of the breeding boar, prolongs the breeding life of the breeding boar, and besides, obviously improves the semen quality, the fertility rate and the embryo survival rate of the breeding boar.

The invention relates to an application of schisandrin B in the preparation of a diluted liquid for preserving boar semen. The invention also relates to a diluted liquid for preserving boar semen. The diluted liquid comprises glucose, sodium bicarbonate, sodium citrate, EDTA, critic acid, potassium chloride, gentamycin, and schisandrin B. The effect of the provided diluted liquid for preserving boar semen is good and reliable. The high quality semen stored at a room temperature can be provided for pig artificial insemination. After the boar semen is preserved in the diluted liquid at a temperature lower than 17 DEG C for five days, the semen activity is still above 0.6.

The invention discloses a nutritional antifreeze diluting agent for improving sperm in-vitro storage and a preparation method of the nutritional antifreeze diluting agent. The nutritional antifreeze diluting agent is prepared from carbohydrates, a cytomembrane stabilizing agent, amino acids, Tris, glycerin, citric acid, penicillin, disodium edetate and double distilled water, wherein the pH is 6.9-7.2, and the osmotic pressure is 300mOsM. Sperm motility, acrosome integrity and plasmolemma permeability are all improved to different extents while the sperm malformation rate is decreased, and remarkable effects are achieved. By application of the nutritional antifreeze diluting agent to boar semen, farrowing rate and live farrowing quantity of sows can be increased, and reproductive performances of the sows are improved.

The invention discloses a normal-temperature preservation diluent for seminal fluid of a Guanzhong black pig. The diluent mainly contains the following components: glucose, sodium citrate, ethylenediamine tetraacetic acid, sodium hydrogen carbonate, potassium chloride, kojic acid and epsilon-polylysine. By utilizing the normal-temperature preservation diluent, the seminal fluid of the Guanzhong black pig can be preserved at room temperature for 1-5 days and can meet the artificial insemination requirement. Kojic acid added into the diluent has good antioxidant and protective effects to semens, meanwhile, the use amount of antibiotics during pig-raising production is reduced, and the problem of the drug resistance of bacteria can be effectively alleviated, so that the problem of the preservation of the seminal fluid of the Guanzhong black pig in an artificial insemination technique adopted in a large-scale cultivation process is solved, the feeding of boars is reduced, and the utilization ratio of excellent boar semens is increased. A preparation method of the diluents is simple in operation, low in production cost and convenient for large-scale popularization and application.

The invention discloses a mathematical model for evaluating fertilization abilities of landrace boars and an establishing method for the mathematical model. The establishing method comprises the steps of: firstly, performing data description on predictive variables of data samples of the landrace boars; secondly, performing system clustering, and dividing the landrace boars with different fertilization abilities according to sample data related with fertilization abilities of boar seminal fluids into a boar group with high fertilization ability and a boar group with low fertilization ability; and thirdly, performing discriminant analysis and Logistic regression analysis after clustering to generate a specific mathematical function. The coefficient and prediction accuracy of the mathematical model established by the method are reliable; a function model suitable for evaluating the fertilization abilities of the in-service landrace boars in Guangdong province can be provided; the function model can serve as a basis for evaluating the fertilization abilities of the landrace boars; and the mathematical model is of great significance on reduction of a gradient change interval of the fertilization abilities of the boars and accurate prediction of the fertilization abilities of the landrace boars.