16 results about "Boar semen" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The utility model discloses a boar semen storage device relates to the field of veterinary device, including the rotation connection's casing one and casing two, casing one inner ring array is equipped with several with the liquid collecting groove intercommunication's pipe connecting assembly, install the negative pressure adsorption subassembly of the semen in the liquid collecting groove in succession suction into several storage test tubes on the direction switch subassembly, the boar semen is introduced into the liquid collecting groove, and the semen in the liquid collecting groove is introduced into the storage test tube that inserts in the corresponding sleeve in the rotation direction switch subassembly, through the direction switch subassembly in succession boar semen is introduced into several storage test tubes, so this can complete the independent storage of multiple semen, and each sleeve forms the sealed cavity, and through the rotation control of the direction switch subassembly has realized the convenient switching of the separation process, and has ensured that the semen in each sleeve does not mix each other, and is saved separately, and adapts the requirement of the independence of semen, safety when subsequent storage or carrying.

The present application relates to a kind of full-automatic boar semen collection device and system, including prosthesis unit, vaginal unit, audio unit, collection unit and control unit. Wherein, prosthesis unit has inner cavity element;Vaginal unit is arranged at the tail of prosthesis unit;Audio unit is arranged at the head position of prosthesis unit, for simulating the sound that the sow handling estrus occurs;Collection unit is arranged in inner cavity element, for collecting the semen that flows from the end of vaginal element;Control unit is connected with vaginal unit, audio unit and collection unit communication respectively.Its advantages are that, through vaginal element simulation sow's real vaginal environment, additional operation difficulty of the whole semen collection process can be reduced;Semen of boar ejaculatory early stage and semen of ejaculatory middle and late stage can also be distinguished and collected, improve the quality of semen collection.

The application provides a boar semen cryopreservation agent and an application method; the boar semen cryopreservation agent comprises alpha-amylase, and the content of the alpha-amylase is 10-1000 mu g / L. The application method of the cryopreservation agent is as follows: centrifuging the boar semen after collection and pretreatment, removing supernatant after centrifugation; adding a refrigerant, re-suspending the sperm, and then placing the sperm in a 4 DEG C refrigerator and standing for 3-4 hours; adding a freezing liquid and fully mixing; filling the semen into a thin tube at 4 DEG C, uniformly laying the thin tubes in a freezing rack, and then precooling on a liquid nitrogen surface and then placing in liquid nitrogen for freezing. The application has the advantages that: the overall components of the cryopreservation agent are simple, the cost is low, and the overall cost performance is high; due to the addition of alpha-amylase in the cryopreservation agent, the storage quality of the boar frozen semen can be significantly improved; and the activity, acrosome integrity, DNA integrity, mitochondrial activity and other physical and chemical properties of the boar frozen semen are effectively improved.

The invention discloses a long-distance transportation low-density boar semen diluent which comprises a basic component and a stabilizer, and the stabilizer is bovine serum albumin; the pH value of the boar semen diluent is 6.3-7.6, the osmotic pressure of the boar semen diluent is 280-370 mOsm, and the ionic strength of the boar semen diluent is 0.2-0.5 mol / L. According to the invention, bovine serum albumin is taken as a core stabilizer, physical and chemical balance is realized through accurate proportioning, and the problems of weak anti-vibration capability, insufficient component synergism and the like of the existing diluent are solved. Researches show that by applying the diluent, the adverse effect of long-distance transportation on the low-density semen of the pigs can be resisted, the survival ability and fertilization potential of the low-density semen after being transported for 24-48 hours can be effectively improved, and the diluent is simple and convenient in preparation and use steps, does not need professional equipment, is controllable in cost, is adaptive to large-scale breeding and transportation scenes, and is suitable for large-scale breeding and transportation. Furthermore, the cross-regional utilization efficiency of the semen of the excellent breeding boars can be improved, the breeding boar feeding cost is reduced, the transmission range of excellent genes is accelerated, and the method has great application value in large-scale live pig breeding.

The utility model relates to the technical field of semen storage, in particular to a boar semen constant-temperature storage device which comprises a constant-temperature box body, a box cover is rotatably connected to the top of the constant-temperature box body, a control panel is arranged on the side wall of the constant-temperature box body, sliding grooves are formed in the two sides of the inner wall of the constant-temperature box body, and sliding plates are slidably connected into the sliding grooves. A supporting plate is fixedly connected between the opposite faces of the sliding plates, a shaking mechanism used for shaking a test tube storing boar semen is arranged at the top of the supporting plate, and the shaking mechanism comprises supporting frames fixedly connected to the two sides of the top of the supporting plate. According to the method, accumulation of metabolic waste and gathering and adhesion of sperms in a standing state are effectively reduced, so that sperm decline is delayed, a more stable and more efficient condition is provided for long-term preservation of the boar semen, long-term high-quality semen preservation is facilitated, and the utilization rate and effectiveness of the semen are improved.

The invention relates to the technical field of animal breeding, in particular to a boar semen improved feed additive based on a herba epimedii compound as well as a preparation method and application of the boar semen improved feed additive. The improved feed additive is prepared from the following active ingredients in parts by weight: 15 to 35 parts of herba epimedii extract, 10 to 25 parts of radix salviae miltiorrhizae extract, 8 to 20 parts of radix rehmanniae extract, 5 to 15 parts of caulis sinomenii extract, 0.1 to 0.8 part of vitamin E and 0.05 to 0.2 part of organic zinc. According to the invention, multiple active ingredients such as the herba epimedii extract, the organic zinc, the radix salviae miltiorrhizae, the radix rehmanniae and the caulis sinomenii are systematically matched with a preferred carrier, so that multi-path synergy in the aspects of spermatogenesis promotion, oxidation resistance, inflammation resistance, endocrine regulation, preparation delivery and the like is realized, and the semen quality of boars is remarkably improved.

The utility model relates to a boar semen collection frame for live pig breeding. Comprising a base plate, a fake sow table and an auxiliary climbing mechanism. A height adjusting electric telescopic rod is arranged at the right end of the base plate, a fake sow table is arranged on a telescopic rod of the height adjusting electric telescopic rod, a front leg lapping rod capable of moving left and right is slidably installed on the fake sow table, and the front leg lapping rod is driven by a left-right adjusting electric telescopic rod arranged in the fake sow table to move; the base plate is provided with an auxiliary climbing mechanism located on the left side of the fake sow table. According to the artificial sow platform, boars can be helped to climb the artificial sow platform through the auxiliary climbing mechanism, meanwhile, the artificial sow platform can ascend and descend, the artificial sow platform can be suitable for boars of different sizes to use, meanwhile, the boars can climb at a short position, then the artificial sow platform is lifted to the designated height, workers can conveniently conduct semen collection work, and in addition, the artificial sow platform is convenient to use. The boars can be assisted to climb towards the right side of the fake sow table through the front leg lapping rods capable of moving left and right, and it is ensured that the boars can climb in place.

The utility model discloses a boar semen incubator, and relates to the technical field of semen storage. The boar semen incubator comprises a box body, a cover is movably installed on one side of the top of the box body through a hinge, an auxiliary fixing mechanism used for conducting auxiliary fixing on boar semen storage test tubes is fixedly installed on the top of the cover, and an extending protection mechanism is fixedly installed on one side of the bottom in the box body. The horizontal state of the device is monitored in real time through power-on operation of an inclination sensor, and when the inclination sensor monitors that the horizontal state of the device exceeds a preset value of the inclination sensor, the inclination sensor controls a first electromagnetic valve pipe to be powered on to be opened, so that high-pressure air in an air tank is conveyed into an air bag, and the air bag expands in volume; the outer side of the device can be protected through the air bag with the expanded volume, the effect of buffering the impact force generated by falling and toppling of the device is achieved, and the function of safety protection of the boar semen test tube is achieved.

The invention belongs to the technical field of biology. The invention relates to an antioxidant composition, in particular to an antioxidant composition and application thereof in normal-temperature preservation of boar semen. Researches find that the activity of boar sperms in the normal-temperature preservation period can be effectively improved by independently adding lycopene or antioxidant SKQ1, the combined use effect of lycopene and antioxidant SKQ1 shows a synergistic effect, and when the boar sperms are preserved to the seventh day, the average sperm activity value exceeds 70%. In addition, through combined treatment, the integrity of sperm acrosome and plasma membrane is remarkably maintained, the antioxidant enzyme activity of sperms is improved, the active oxygen and malonaldehyde level are effectively reduced, meanwhile, the mitochondrial membrane potential and the adenosine triphosphate content are remarkably improved, and the expression level of sperm P-AMPK and Nrf2 protein is improved. In conclusion, the lycopene and the SKQ1 are combined for use, oxidative stress and energy metabolism are synergistically regulated and controlled, the quality and function stability of the boar sperms in the normal-temperature preservation process are remarkably improved, and the lycopene and the SKQ1 have good application value and application prospects.

The invention relates to an additive of a diluent for preserving boar semen at 17 DEG C and an application method thereof. Myoinositol with the concentration of 1 mg / mL is added into a boar semen diluent, so that the activity and forward movement proportion of boar sperms under the preservation condition of 17 DEG C can be remarkably improved, and meanwhile, the lipid oxidation state and acrosome integrity of the boar sperms are not influenced. The method is easy and convenient to operate, low in cost, suitable for the large-scale pig farm artificial insemination technology and beneficial to prolonging the pig semen preservation time and improving the breeding efficiency.

The invention discloses a diluent for normal-temperature preservation of boar semen and a preservation method. Selenomethionine is selected as a protective agent in the diluent for preserving the boar semen at the normal temperature to improve the semen quality, the diluent for preserving the semen at the low temperature is a Modena basic diluent, and the addition amount of the selenomethionine in the diluent for preserving the semen at the normal temperature is 0.1-10 mg / L, preferably 1 mg / L. According to the invention, selenomethionine is added into the diluent for preserving the boar semen at normal temperature, so that the motility, motility rate, plasma membrane integrity rate and acrosome integrity rate of the boar semen can be remarkably improved. If the method is applied to actual production, the normal-temperature preservation time of the boar semen can be prolonged, the semen preservation quality is improved, and the production cost is reduced.

The application relates to an automatic boar semen collection device and system, which comprises a sow prosthesis, a bionic vagina, a sound simulation device, a cleaning device, a semen collection device and a control device. The bionic vagina is arranged at the tail of the sow prosthesis, and the tail end of the bionic vagina extends into a cavity. The sound simulation device is arranged at the head of the sow prosthesis and is used for simulating the sound generated by the sow during estrus. The cleaning device is arranged at the tail of the sow prosthesis and is used for flushing the penis of the boar. The semen collection device is arranged in the cavity and is used for collecting the semen flowing out of the tail end of the bionic vagina. The control device is in communication connection with the bionic vagina, the sound simulation device, the cleaning device and the semen collection device. The device has the advantages that the real vaginal environment of the sow can be simulated through the bionic vagina, the difficulty of the whole semen collection process can be reduced, and the semen in the initial ejaculation stage and the semen in the middle and later ejaculation stages of the boar can be collected separately, so that the quality of the semen collection is improved.

The invention relates to the technical field of livestock breeding, and discloses a novel pig semen cryopreservation technology and a special low-temperature protective agent thereof. The formula of the special low-temperature protective agent comprises the following components: basic liquid: glucose, sodium citrate, Tris, sodium bicarbonate, potassium chloride, a pullulan grafted manganese-based complex, taurine and deionized water; the antioxidant is prepared from vitamin E, glutathione, melatonin and a rosemary extract; the cryoprotectant comprises glycerol and dimethyl sulfoxide; nutritional additives: fresh egg yolk and trehalose; and antibiotics: penicillin sodium, streptomycin sulfate and lactoferrin. The cryopreservation technology comprises the following steps of semen collection and pretreatment, protective agent addition and balance, freezing and preservation, and unfreezing and insemination.

The invention discloses a semen diluent for improving the production efficiency of pig semen, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises an antioxidant and a composite antibacterial agent, the nutritional agent is composed of glucose and fructose, the antioxidant is glutathione, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. Accordingly, a corresponding preparation method and a using method are also established. Researches show that the diluted boar semen can survive at 17 DEG C for more than or equal to 144 hours, the activity is more than or equal to 60%, 1 part of the original semen with the sperm density of 200 mL and the sperm density of 1 billion / mL can cover 40 sows, the utilization rate of the original semen is increased by 150%, the breeding cost is remarkably reduced, and the method is suitable for large-scale popularization.

The invention discloses application of BSA (Bovine Serum Albumin) in improving the performance of low-density boar semen, and the BSA is particularly suitable for 0.5 billion / 60ml low-density boar semen. Accordingly, the inventor also develops a boar semen diluent, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises a core film protecting agent, an antioxidant and a composite antibacterial agent, the core film protecting agent is BSA, the nutritional agent is composed of glucose and fructose, the antioxidant is composed of glutathione and arbutin, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. By accurately optimizing the BSA addition concentration and cooperating with glucose and fructose (dual-energy supply), a high-quality sperm protection microenvironment system of'targeted film protection-environment stabilization-energy supply 'is constructed, the problems of short preservation time and poor sperm form integrity when low-density sperms are treated by conventional diluents are solved, and cost reduction and efficiency improvement of large-scale pig farms can be realized.