10 results about "Galangin" patented technology

The present invention relates to a composition for protecting cells from endoplasmic reticulum (ER) stress and oxidative stress induced by ultrafine dust, comprising galangin as an active ingredient. Galangin, which is an active ingredient of the composition for cell protection of the present invention, alleviated an increase in reactive oxygen species, intracellular calcium, and ER stress-related proteins induced by ultrafine dust PM2.5. In addition, galangin counteracted PM2.5-induced cell cycle arrest at the G0 / G1 phase and restored cell cycle regulators and matrix metalloproteinases. As a result, galangin can enhance cell protection against PM2.5-induced ER stress and cell cycle arrest via reactive oxygen species. Therefore, it is suggested that the composition comprising galangin as an active ingredient of the present invention has great potential as a candidate for a preventive agent or a therapeutic agent for diseases related to ER stress and oxidative stress induced by PM2.5.

The invention relates to the technical field of biological medicines, in particular to a galangal-sourced nano-vesicle as well as a preparation method and application thereof. The method specifically comprises the following steps: crushing rhizoma alpiniae officinarum rhizomes, mixing the crushed rhizoma alpiniae officinarum rhizomes with a buffer solution, soaking in a water bath, and filtering to remove solids so as to obtain a crude extract; and sequentially carrying out centrifugal treatment on the crude extract for four times, so as to obtain the galangal-derived nano-vesicles. The invention provides a method for extracting nano-vesicles from galangal, and finds for the first time that the obtained vesicles can promote osteogenic differentiation of human bone marrow mesenchymal stem cells with high efficiency and low toxicity, the effect of the vesicles is obviously superior to that of known active component galangin, and the cytotoxicity is obviously lower; the invention provides raw materials and methods with great potential for developing novel and safe anti-osteoporosis drugs or inducers.

The invention belongs to the technical field of nano biomedicine, and particularly discloses a preparation method of a galangin-loaded bionic nano dendritic cell (DCs) vaccine and application of the galangin-loaded bionic nano dendritic cell (DCs) vaccine in ovarian cancer treatment. The inner core of the vaccine is galangin (GA)-loaded polylactic acid-glycolic acid copolymer (PLGA) nanoparticles, and the shell of the vaccine is formed by coating a DCs film carrying tumor antigens. The nano DC vaccine retains the antigen presentation function of natural DCs, and the GA loaded on the nano DC vaccine can induce tumor cells to generate immunogenic cell death. By means of the design, the vaccine can efficiently load specific tumor-associated antigens, and the homing capacity of the vaccine to lymph nodes and tumor microenvironments is remarkably enhanced. Experiments show that GA-NPs (at) DCV is used for treating subcutaneous tumor and in-situ ovarian cancer mouse models.

The invention relates to the technical field of ultrasonic extraction, and discloses galangin ultrasonic extraction equipment which comprises an extraction tank and an ultrasonic vibration rod, the extraction tank comprises an outer tank body and an inner filtering tank, the inner filtering tank is located in the outer tank body, a rotating cover is arranged on the inner filtering tank, the ultrasonic vibration rod is arranged below the rotating cover, and the ultrasonic vibration rod is arranged below the outer tank body. The ultrasonic vibration rod can move relative to the rotating cover, in the ultrasonic extraction process, the rotating cover drives the ultrasonic vibration rod to rotate in the inner filtering tank, and the ultrasonic vibration rod can move along the diameter of the rotating cover relative to the rotating cover; according to the technical scheme, the ultrasonic vibration rod is arranged in the inner filtering tank, the movement track of the ultrasonic vibration rod can spread all over the whole cavity of the inner filtering tank, meanwhile, the ultrasonic vibration rod can be attached to the inner wall of a tank body of the inner filtering tank, and the inner filtering tank is driven to vibrate through the ultrasonic vibration rod. Meanwhile, the filtering efficiency after extraction can be accelerated.

The invention belongs to the technical field of biological medicines, and particularly relates to application and a method of flavonoid compounds in enhancing nucleic acid molecule delivery efficiency of lipid nanoparticles. The invention aims to solve the problems of limited LNP in-vivo and in-vitro delivery efficiency, toxic risk and the like. According to the invention, seven low-toxicity and biocompatible natural compounds such as genistein, glabridin, rhoifolin, luteolin, hesperetin, galangin or pinocembrin are used as an LNP high-efficiency expression enhancer, the compounds and target cells are co-incubated, and then LNP containing nucleic acid molecules is added. According to the scheme, the LNP-mediated gene expression efficiency can be remarkably improved, the cytotoxicity can be reduced, and in-vivo experiments prove that LNP biological distribution can be optimized, target tissue expression can be enhanced, and collaborative optimization of LNP safety and high efficiency is achieved.

The invention relates to the technical field of food processing, in particular to a preparation method and application of zein-citrus pectin nanoparticles loaded with galangin. The preparation method comprises the following steps: providing a zein solution, and carrying out first mixing treatment on galangin powder and the zein solution to obtain a galangin-zein solution; dropwise adding the galangin-zein solution into a continuously stirred water phase, and concentrating to remove ethanol, so as to obtain a galangin-loaded zein nanoparticle dispersion liquid; providing a citrus pectin solution, carrying out second mixing treatment on the citrus pectin solution and the zein nanoparticle dispersion liquid loaded with galangin, and then carrying out post-treatment to obtain the zein-citrus pectin nanoparticles loaded with galangin, the nanoparticles are applied to the sauced products for the first time, the heterocyclic amine content in the sauced meat products is effectively reduced, and the safety of the sauced meat products is improved.

The invention provides application of gallic acid combined galangin in improving body color and muscle flavor of plectropomus latifolius, and belongs to the technical field of development of aquatic feed additives. After gallic acid and galangin are combined according to the mass ratio of (1-3): (1-3), the body color of the red pink bass can be effectively improved, the content of flavor amino acid in muscular tissue of the red pink bass is increased, and on the basis, the invention provides a feed additive, a red pink bass feed and a method for breeding the red pink bass by adopting the red pink bass feed. The feed cost and the breeding cost can be effectively reduced, and the body color and the muscle flavor of the red plectropomus japonicus can be improved.

The present application relates to the technical field of honey detection, and particularly relates to a vitexin, a preparation method thereof and a method for identifying vitex honey. The vitexin has a structure shown in formula 1: The present application finds a new compound, vitexin, in vitex honey through research on the vitex honey. The present application also finds that when the to-be-tested honey contains vitexin (p-hydroxybenzoic acid 1-O-gentio-bioside-5-hydroxy-3,7-diene-[2,6] cyclopentanopyranyl-3-methyl ester), or contains vitexin and new chlorogenic acid, 4-hydroxybenzoic acid, chlorogenic acid, caffeic acid, p-coumaric acid, red flower phaseolus acid, trans, trans-epiabscisic acid, cis, trans-epiabscisic acid, 5-methoxy pinocembrin, pinocembrin, kaempferol, pinocembrin, benzyl caffeate, kojic acid, galangin at the same time, the to-be-tested honey can be determined as vitex honey. The present application also provides a new method for constructing a vitex honey authenticity and quality evaluation system.

The application discloses a self-assembled nano drug of berberine and galangin, which comprises berberine and galangin, and the molar ratio of the berberine and the galangin is 1:1-2. The berberine and the galangin can be self-assembled through a pi-pi bond, electrostatic force and a hydrogen bond to form the self-assembled nano drug, so that the problem of poor water solubility of single berberine and single galangin is improved. Meanwhile, when the self-assembled nano drug is applied to a mouse model of ulcerative colitis, compared with single berberine or single galangin, the self-assembled nano drug significantly relieves the colon shortening, the increase of disease activity index and the intestinal pathological damage phenomenon of the mouse with ulcerative colitis caused by sodium sulfate.

The application discloses a propolis extract and a production process thereof. According to mass percentage, the propolis extract comprises 25-40% of total flavones, 15-30% of total phenolic acids, 10-20% of propolis anthracene acid compounds, ≤3% of moisture, ≤2% of ash, and the balance of other natural propolis components; and the sum of acacetin, galangin and pinocembrin in the total flavones accounts for 30-50% of the mass of the total flavones. The production process comprises freezing and crushing, complex enzymolysis, ultrasonic-assisted ethanol extraction, dewaxing, macroporous adsorption resin purification and drying. The application combines enzymolysis and ultrasonic to significantly improve the extraction rate and purity of active ingredients, and the obtained propolis extract has clear components, high active ingredient content, few impurities, and the process is green and efficient, and is suitable for industrial production.