Composition For Diagnosing Skin Damage Caused By Fine Dust, And Composition Comprising Galangin As Active Ingredient

A technology of composition and fine dust, which is applied in the direction of medical preparations containing active ingredients, screening of compounds, organic active ingredients, etc., can solve the problems of relaxation, inactivity, and lower water content of the stratum corneum, and achieve the purpose of strengthening the skin barrier, Effect of improving skin damage and promoting differentiation of keratinocytes

Active Publication Date: 2018-03-13
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to various factors under environmental or lifestyle conditions, including artificial temperature control by heating/cooling, skin stress caused by various social pressures and environmental ...

Method used

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  • Composition For Diagnosing Skin Damage Caused By Fine Dust, And Composition Comprising Galangin As Active Ingredient
  • Composition For Diagnosing Skin Damage Caused By Fine Dust, And Composition Comprising Galangin As Active Ingredient
  • Composition For Diagnosing Skin Damage Caused By Fine Dust, And Composition Comprising Galangin As Active Ingredient

Examples

Experimental program
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Effect test

Embodiment 1

[0113] [Example 1] Collection and extraction of fine dust.

[0114] Dust was collected using a low volume sampler (Sensidyne, Gillian, Low Volume Air Sampler, FL, USA). Sampling was continued for about 24 hours, and the filter and the diffusion tube of the filter group were replaced at about 10 am on the day of sampling. From February 1, 2014 to February 28, 2014, fine dust was collected daily in the downwind area of ​​Seoul (Yongin City, on the roof of a six-story building), Korea. Sampling time was recorded by using a timer to detect when the vacuum pump was on. At the start and finish of sampling, a flow meter (Model 4143, TSI Inc.) was used to measure the sampling rate set at 16.7 L / min. Before and after sampling, the Teflon filter (Teflon filter) loaded in the filter group was weighed. Before weighing the Teflon filter, it was set in a desiccator (Nikko, Japan) with a relative humidity of 40%. The weight was measured twice using an electronic balance (DVG215CD, Ohaus)...

Embodiment 2

[0115] [Example 2] Culture of human normal keratinocytes

[0116] Human normal keratinocytes (epidermal new keratinocytes) purchased from Lonza, Inc. (Walkersville, MD, USA) were incubated at 37°C and 5% CO 2 conditions, in CO 2 Cultivated in an incubator. Cells were cultured according to the protocol of Lonza, Inc. Using KGMTM-2Bullet Kit CC-3107 (ingredients: BPE (bovine pituitary extract), human epidermal growth factor (hEGF), insulin, hydrocortisone, transferrin, epinephrine and GA-1000 (gentamycin sulfate + Amphotericin B)), wherein KGM-2Bullet kit CC-4152 was added to 500 m of KBM-2 (KBMTM-2, CC-3103) medium.

Embodiment 3

[0117] [Example 3] Treatment of human normal keratinocytes with fine dust and measurement of cytotoxicity

[0118] In order to investigate the cytotoxicity of fine dust, MTT assay was performed using human normal keratinocytes according to the method of Mossman et al. (J. Immunol. Methods, 65, 55-63, 1983).

[0119] Specifically, a 24-well plate was used, and the fine dust with a particle diameter of 10 μm and the fine dust with a particle diameter of 2.5 μm obtained in Example 1 were respectively dispersed in purified water. The 2.5x10 cultivated under the condition of embodiment 2 5 Each cell of individual normal keratinocytes was treated with the prepared microdust dispersion, cultured for 24 hours, and added 5 mg / mL MTT (3-4,5-dimethylthiazole-2,5-diphenyltetra After the azole bromide salt, the cells were further incubated at 37°C for 3 hours. Then, the medium was removed, and the formed formazan crystals were dissolved in 500 μL of DMSO. The dissolved formazan crystals w...

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Abstract

The present specification discloses a biomarker for diagnosing skin cell damage caused by fine dust, a kit using the same, and a novel use of a composition comprising galangin as an active ingredient.Specifically, it is possible to conveniently check skin damage caused by fine dust by measuring and comparing the expression amounts of the biomarker in normal cells and cells damaged by fine dust. In addition, a composition of the present invention comprising galangin, an isomer thereof, a pharmaceutically acceptable salt thereof, a prodrug thereof, a hydrate thereof or a solvate thereof as an active ingredient can normalize the gene expression of skin damaged by fine dust and promote differentiation of skin keratinocytes, thereby having a skin moisturizing or skin-barrier strengthening effect. Accordingly, since the composition can be used to prevent, treat, or alleviate skin diseases such as atopy, psoriasis, etc. the composition is useful.

Description

technical field [0001] The present invention discloses a biomarker that can be used for diagnosing skin damage caused by fine dust, a composition containing the biomarker, a kit containing the biomarker; and a novel composition containing galangin as an active ingredient. use. 【Background technique】 [0002] In the skin, the epidermis plays an important role in preventing water from evaporating from the body. The epidermis of the skin is composed of the stratum corneum, granular layer, spinous layer, and basal layer from the outside. The cells of the stratum corneum, or keratinocytes, are embedded like bricks in a lipid matrix, which acts like a mortar, thereby constituting the skin barrier (J. Invest. Dermatol. 80 (Suppl.)), 44-49.1983). In the keratinocytes of healthy people, natural moisturizing factors are present in high concentrations to keep the skin moisturized. For example, water-soluble substances such as amino acids easily absorb moisture and prevent skin dryne...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68A61K8/49A61Q19/00A61Q17/00A61K31/352A61P17/00
CPCA61K8/498A61K31/352A61Q17/00A61Q19/00C12Q1/6883C12Q2600/158G01N33/68A61K8/49C12Q1/68G01N33/50A61P17/00A61Q19/10C12Q2600/136G01N33/6893G01N2500/00G01N2800/20A23L33/10A23V2002/00A23V2200/318A23L33/30
Inventor 金亨俊车娜悧朴主烈郑昌朝权里畊申东旭李泰龙
Owner AMOREPACIFIC CORP
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