403 results about "Normal cell" patented technology

The invention relates to the field of molecular targeted therapy, and discloses an application of an SLC16A5 (MCT6) small-molecule inhibitor MCT6-Ai7-2 in preparation of a medicine for treating acute myeloid leukemia (AML). The inhibitor takes an SLC16A5 protein structure predicted by Alphafold as a target spot, and is obtained through compound database screening, molecular docking and druggability optimization. An in-vitro experiment proves that MCT6-Ai7-2 can remarkably inhibit proliferation of AML cell lines such as U937 and MOLM-13, induce cell apoptosis and retard a cell cycle, has an inhibiting effect on a primary AML patient specimen and is relatively low in toxicity to normal cells; in-vivo experiments show that the compound is effective and has good safety in AML model mice. In addition, the MCT6-Ai7-2 and the vinca can be combined to synergistically enhance the inhibition effect on vinca drug-resistant cells, and by reducing the expression of anti-apoptotic protein MCL-1, the activation of pro-apoptotic factors BIM and tBID is promoted to play a role. The invention provides a novel targeting drug and strategy for treatment of AML (especially drug-resistant or recurrent patients).

The invention discloses a multiple myeloma bone marrow image recognition system based on computer vision, relates to the technical field of cell recognition, solves the problem that traditional manual microscopic examination is large in limitation, and accurately recognizes burr change segments by conducting angle analysis on the edge contour of a feature area, so that the accuracy of recognition is improved. According to the comprehensive proportion of the burr change section on the overall edge contour, reliably judging whether the to-be-detected cell is a tumor cell or not, and calibrating and displaying the tumor cell in a gray image; according to the mode, the obvious difference of the tumor cells and normal cells in cell nucleus morphology is effectively utilized, rapid and accurate identification of the tumor cells is achieved, a visual and clear result is provided for clinical diagnosis, doctors are assisted to accurately judge multiple myeloma in time, the diagnosis efficiency and the treatment effect are improved, mean value processing and radial ratio analysis are conducted on contour area gray values, and the diagnosis accuracy is improved. The contour region associated with the cell nucleus can be accurately locked from the to-be-detected cell and is calibrated as the feature region, and a key cell nucleus feature basis is provided for tumor cell recognition.

Methods and systems for processing a scanned tissue section include locating cells within a scanned tissue. Cells in the scanned tissue are classified using a classifier model. A tumor-cell ratio (TCR) map is generated based on classified normal cells and tumor cells. A TCR isoline is generated for a target TCR value using the TCR map, marking areas of the tissue section where a TCR is at or above the target TCR value. Dissection is performed on the tissue sample to isolate an area identified by the isoline.

The invention discloses an early diagnosis marker for B-cell lymphoma, a cross-species screening method based on lamprey and application, and relates to the technical field of molecular diagnosis. In the prior art, tissue biopsy is strong in invasiveness, and a traditional marker is low in early detection rate; in order to solve the problems that a large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large- When the expression quantity of the gene in a sample is greater than or equal to 1.8 times of that of a normal B cell (HMY2. CIR), the lymphoma is judged to be positive, and the minimally invasive early diagnosis efficiency is remarkably improved.

The invention relates to a three-level lymphoid structure model and a preparation method thereof, and belongs to the technical field of cell biology. The method for preparing the three-stage lymphoid structure model comprises the following steps: S1, inducing and activating peripheral blood mononuclear cells by using an extracellular matrix to obtain activated peripheral blood mononuclear cells; s2, the peripheral blood mononuclear cells and the mesenchymal stem cells activated in the step S1 are coated with hydrogel, and hydrogel microspheres are obtained; and S3, culturing T cells and B cells by using the hydrogel microspheres obtained in the step S2 to obtain the three-stage lymphatic structure. The three-level lymphoid structure model obtained by the invention maintains long-term stability in vitro, the form is normal, and the activity of T cells and B cells is strong. The method can be used for dynamically observing the three-level lymphoid structure in the subsequent research immunotherapy and drug screening process, is used for high-throughput experiments, effectively improves the efficiency of drug screening experiments, and is high in accuracy.

The invention discloses an anti-CD24 antibody and an application thereof. Specifically disclosed is an anti-CD24 antibody or antigen-binding fragment thereof, comprising a heavy chain variable region (SEQ ID NO: 1, 5, 9 or 13) and a light chain variable region (SEQ ID NO: 3, 7, 11 or 15). The anti-CD24 antibody (including a mouse monoclonal antibody and three humanized monoclonal antibodies) is obtained through a hybridoma technology and a CDR transplantation method. The anti-CD24 antibody has high affinity, can significantly inhibit the growth of tumors, has a tumor inhibition rate of 82.3%, has a significant anti-tumor curative effect, has a strong binding capacity with various tumor cells and tissues, does not bind with normal cells, and has good tumor selectivity. The antibody can be prepared into prevention and treatment drugs, diagnosis drugs, detection kits and the like for CD24 target related diseases, and has very wide clinical application prospects in the fields of tumor treatment and diagnosis and the like.

The invention relates to the technical field of biological medicines, and particularly discloses a transcription factor HOMEZ phosphorylation site specific antibody as well as a preparation method and application thereof. The specific antibody provided by the invention can specifically recognize phosphorylation of the 451st site threonine of HOMEZ in a tumor specimen, so that the prognosis condition of the tumor is judged, and a potential action target is provided for clinical diagnosis and treatment of the tumor; moreover, the specific antibody disclosed by the invention can be used for detecting the expression difference between normal cells and tumor cells in a Western blot experiment, and is beneficial to research on the effect of phosphorylation modification of HOMEZ protein in the occurrence and development process of tumor diseases; and the kit can also be used for detecting the phosphorylation level of the HOMEZ protein in a clinical specimen through experiments such as immunofluorescence and immunohistochemical staining, and has a wide clinical application prospect in the aspects of disease diagnosis, treatment, prognosis judgment and the like.

The invention relates to the technical field of image processing, in particular to a stomach cancer tissue typing auxiliary recognition method based on digital pathological sections. According to the method, morphological feature recognition is carried out on edge information in a grayscale image of a slice, and normal cell edges and abnormal cell edges are screened out according to the size of morphological feature indexes and the morphology of a cell edge curve. A first abnormal area and a second abnormal area are screened out according to the proximity relation, then enhancement coefficients of the two abnormal areas are calculated and enhanced, and a gastric cancer tissue typing recognition result is obtained according to the enhanced stomach pathology image. According to the invention, the object of the enhancement process is only limited to the abnormal region, and different degrees of enhancement are realized for different types of abnormal regions, so that the calculation amount of the enhancement process is reduced, the enhancement process has pertinence, excessive enhancement cannot be caused, and a clear slice image is obtained for tissue typing identification; and the tissue typing recognition efficiency is improved.

The application discloses a cell detection method and system based on deep learning and machine vision, which comprises the following steps: cutting a cervical liquid-based cell pathology digital image into a plurality of sub-images to form a sub-image set; inputting the sub-image set into a normal cell detection model and a lesion cell detection model respectively, and identifying normal cells and lesion cells and their categories; selecting an overlapping area of the normal cells and the lesion cells, and performing secondary classification on the overlapping area through a classification model; performing threshold segmentation on granulocytes in the cervical liquid-based cell pathology digital image to obtain all possible granulocyte targets, filtering to determine final granulocyte targets, and counting and determining the number of the granulocytes. The application solves the problem that the detection model in the prior art has poor detection effect due to the imbalance of normal cell and lesion cell categories, improves the accuracy of cell identification, and realizes accurate detection and identification of cervical liquid-based cells in combination with accurately identified granulocytes.

The invention belongs to the technical field of analytical chemistry, and relates to a method for identifying and detecting endogenous mercaptan of a high-activity oxidized nano-enzyme based on machine learning, which comprises the following steps: firstly, preparing a Mnx (DTPMP) nano-enzyme, then constructing a sensor array of one nano-enzyme, four reaction times, three targets and six parallel samples, and detecting the endogenous mercaptan in the Mnx (DTPMP) nano-enzyme. Constructing a sensor array of one nano enzyme, four reaction times, 7 concentrations and 6 parallel samples for each endogenous mercaptan; and finally, analyzing the data through hierarchical clustering analysis, linear discriminant analysis and a support vector machine to obtain an LDA score chart, an HCA map, an SVM confusion matrix and a concentration prediction map of the endogenous mercaptan. After a sample to be detected is subjected to a chromogenic reaction, the absorbance is measured, and the type and concentration of the endogenous mercaptan of the sample to be detected can be distinguished according to the obtained spectrum. The kit has good anti-interference performance, normal cells and cancer cells can be accurately identified according to the content of glutathione in the cells, and the severity of diseases can be judged according to the content of homocysteine in serum.

The invention discloses a transparent culture device supporting cell orientation and electrophysiological signal in-situ monitoring and a preparation method. The transparent culture device comprises a transparent substrate, a microelectrode structure is prepared on the transparent substrate, oriented biological fibers are prepared on the microelectrode structure, and a cell culture cavity used for containing cell culture fluid is formed above the oriented biological fibers and the microelectrode structure; the directional biological fiber and microelectrode structure arrangement mode is designed according to requirements and is printed and formed, and a transparent substrate material is selected according to requirements; according to the invention, cell orientation and electrophysiological signal in-situ monitoring are supported on the transparent culture surface through electrostatic printing, and normal cell observation is not affected.

Machine learning techniques of characterizing senescence in an immobilized patient cell are disclosed. The techniques include: obtaining a patient cell image; passing the patient cell image to a trained machine learning system, where the trained machine learning system is trained using a training corpus that includes a plurality of training cell images, where the plurality of training cell images include a plurality of images of normal cells and a plurality of images of cells with senescence; obtaining an output from the trained machine learning model in response to the passing, where the output is indicative of a senescence property of the patient cell, and where the senescence property is non-binary; and providing an indication of the senescence property of the patient cell based on the output.

A memory device includes a memory cell array including normal cell blocks and at least one error correction code (ECC) cell block, which include a plurality of rows; a scrub control circuit configured to perform an error check operation on each of the plurality of rows; and a write-back prevention circuit configured to store, based on local error signals for the normal cell blocks and the at least one ECC cell block during the error check operation, a severe address for a row in which a severe error occurs, and selectively perform a write-back operation on the row corresponding to the stored severe address during the error check operation or a read-modify-write (RMW) operation.

The invention discloses an application of a reagent for detecting the expression of a biomarker AKT3 in preparation of a product for auxiliary diagnosis or diagnosis of a head and neck cancer object, an application of AKT3 as a target in screening of a medicine for treating head and neck cancer, and an application of an AKT3 expression inhibitor in preparation of a medicine for treating head and neck cancer. The invention provides a novel head and neck cancer diagnosis marker AKT3, and compared with normal tissues and normal cells, the marker shows that the expression in the head and neck cancer tissues and cells is obviously up-regulated, and can be used for diagnosing the head and neck cancer; research experiments prove that knock-down of the AKT3 can inhibit migration and invasion of head and neck cancer cells and can inhibit growth of subcutaneous transplantation tumors in mice, and the AKT3 can be used as a treatment target of the head and neck cancer; and a new target and a treatment strategy are provided for the treatment of the head and neck cancer.

The invention discloses a CAIX targeted degradation agent. The CAIX targeted degradation agent comprises a CAIX target protein ligand, an integrin ligand and a connector, the CAIX target protein ligand and the integrin ligand are connected through the connecting body. As the carbonic anhydrase IX is enriched in the tumor region, the CAIX targeted degradation agent can specifically target the tumor region; the compound can effectively induce degradation of CAIX and inhibit survival of tumor cells under the hypoxia condition, has the functions of targeting a tumor area and degrading carbonic anhydrase IX by utilizing integrin and lysosome, further changes hypoxia and subacid environments of the internal environment of a tumor patient, and plays a role in inhibiting survival of hypoxia tumor cells; the tumor targeting property is high, the toxic and side effects on normal cells are relatively small, and a relatively good effect can be obtained by using a relatively small dosage.

The invention relates to a memory including a sense amplifier and a method of operating the memory. The operating method of the memory may include: receiving a first activation command and a first row address; initiating a first mismatch compensation operation in a sense amplifier array for a normal cell array corresponding to the first row address and in a redundant sense amplifier array for a redundant cell array corresponding to the normal cell array; determining whether to access the normal cell array based on the first row address and the repair information; in response to a determination to access the normal cell array, deactivating the redundant sense amplifier array for the redundant cell array; activating a word line of the normal cell array corresponding to the first row address; and sensing and amplifying data of a memory cell corresponding to the activated word line by a sense amplifier array for the normal cell array.

The embodiment of the present disclosure discloses a method for editing the layout of a frozen column of a table, creating a normal table in a webpage, setting a title row as a sticky layout to be fixed in the first row of the normal table, setting a first normal cell as a sticky layout to be fixed in the first column of the normal table, creating a floating row, setting the floating row as an absolute positioning, adding an additional element to a cell of the floating row, adjusting a top offset position of the floating row to be consistent with a top offset position of a second normal cell, adjusting a side offset position of the additional element to be consistent with a side offset position of the second normal cell, setting the additional element as a sticky positioning if the second normal cell is a sticky layout, which can realize code decoupling, reduce the development difficulty of the additional element, make the additional element more accurately attached to the second normal cell of the normal table, and correctly handle the hierarchical coverage relationship between the additional element and the frozen column of the normal table.

The invention relates to a bispecific antibody-cytokine fusion protein as well as a preparation method and application thereof. The fusion protein comprises a TAA binding unit, a T cell binding unit and a cytokine unit, the TAA binding unit comprises an anti-HER2 structural domain, the T cell binding unit comprises an anti-CD3 structural domain, and the cell factor unit comprises IL-2; the anti-CD3 structural domain and the IL-2 structural domain can act on T cells at the same time, further amplification of the T cells is promoted while the T cells are activated to generate cytotoxic killing, the anti-CD3 structural domain and the IL-2 structural domain can be directionally migrated to the periphery of HER2 positive tumor cells through the anti-HER2 structural domain, the efficiency of promoting proliferation and differentiation of tumor infiltrating T cells is improved, and the T cells can be effectively degraded. The effect of killing solid tumor cells by immune cells is greatly improved, the non-specific killing effect of the fusion protein on normal cells and the accompanying release of cytokines in peripheral blood can be reduced to the minimum, and the toxic and side effects of the fusion protein in clinical treatment are reduced.

This application relates to a method, apparatus, and memory for detecting memory failures. The memory failure detection method performs error detection on read data from the memory array to identify failed cells. When repair triggering conditions are met, a repair operation is performed on the failed cell. The success of the repair is verified. If successful, the failed cell is marked as a normal memory cell and continues to be used. If not, redundant cells are used to replace the failed cell. Performing repair operations on failed cells can repair soft-failure cells, avoiding the replacement of a large number of normal cells by redundant cells, thus consuming redundant resources. This allows limited redundant cells to replace unrepairable hard-failure cells, improving the effective utilization rate of redundant cells and preventing redundant resources from being prematurely consumed by repairable soft-failure cells, thus delaying the depletion rate of redundant resources and extending the working life of the memory.

The invention discloses an application of an METTL3 small-molecule inhibitor STM2457 in preparation of a medicine for treating gastric cancer. The STM2457 specifically induces ferroptosis of gastric cancer cells through the m6A-GPX4 axis, compared with RSL3, the STM2457 has the advantages that the toxicity of the STM2457 to normal cells is reduced by about 30%, the targeting property is clear, the curative effect and the safety are both achieved, and a theoretical basis and experimental data support are provided for clinical development of METTL3 targeted drugs.

The invention provides a p53 fusion protein based on a targeted colorectal cancer marker CEA and application of the p53 fusion protein in preparation of medicines for inhibiting colorectal cancer, and relates to the field of biological medicines. The fusion protein comprises any one of the following components: p28-p53, MBP-TEV-p14ARF (1-63)-linker-p28, p28-p53-CEABP1, CEABP1-p28-p53, and CEABP2-p28-p53, and the fusion protein comprises any one component selected from the group consisting of the following components: a protein A, a protein B, a protein A, a protein B, a protein C and a protein B, according to the application, p53 and p14 ARF proteins for inhibiting cell proliferation in a human body, cell-penetrating peptide and designed protein CEABP1 or CEABP2 of a targeted binding colorectal cancer marker CEA are fused for the first time, and cell experiments and mouse experiments prove that the protein has a relatively high function of inhibiting growth of colorectal cancer cells, does not influence normal cell growth and has a wide application value.

The invention relates to a nano AIE probe as well as a preparation method and application thereof. The probe is synthesized by taking a compound A as a raw material and F-127 as a surfactant through a nano precipitation method; the structural general formula of the compound A is # imgabs0, wherein R is # imgabs1 # imgabs2 #, and the preparation method comprises the following steps: S1, preparing the compound A; and S2, preparing the nano AIE probe. The nano AIE probe has AIE characteristics, excellent phototoxicity, good cancer cell specificity, low cell dark toxicity and good biocompatibility, can well distinguish cancer cells from normal cells, can be applied to tumor treatment as a photosensitizer, and has a good application prospect.

Use of a BCL2 inhibitor in the preparation of a drug or medical instrument for treating giant congenital melanocytic nevi (GCMN). The use of the BCL2 inhibitor in GCMN has been validated to exert a therapeutic effect by means of reversing anti-apoptotic response of GCMN and inducing apoptosis of GCMN. In addition, good killing effects have been achieved in both primary GCMN cells from multiple patients and animal experiments, indicating that the BCL2 inhibitor has a strong and specific killing effect on giant nevus cells while exerting minimal impact on other normal cells, thereby achieving precise therapeutic and killing effects to address the predicament of the lack of effective treatment methods for GCMN.

The invention discloses a preparation method and application of programmable DNA hydrogel for inhibiting breast cancer tumor activity. The DNA hydrogel is composed of an RCA long chain AL containing a multivalent AS411 aptamer sequence and an RCA long chain CL hybridized with a cholesterol single chain ss-chol. Through reasonable DNA programming sequence design, a three-dimensional DNA structure can be formed on the surface of the cell. The DNA hydrogel specifically targets cancer cells with high expression of nucleolin receptors through the synergistic effect of multivalent AS1411 and chol, and is weak in targeting to normal cells with low expression of nucleolin, so that toxic and side effects on the normal cells are effectively avoided. The DNA hydrogel has excellent programmability, hydrogel with high, medium and low pore densities can be simply prepared through flexible DNA sequence design, and the breast cancer resistance activity of the DNA hydrogel is in positive correlation with the pore density of the DNA hydrogel. In animal in-vivo experiments, the DNA hydrogel under three-dimensional and high pore density significantly inhibits breast cancer activity, and a new strategy is provided for tumor treatment.

The invention relates to a preparation method and application of selenium-doped carbon quantum dots. The selenium-doped orange fluorescent carbon quantum dots are successfully prepared by treating a mixture of mangosteen peel, ethanol and sodium selenite through a hydrothermal method. Based on the excellent fluorescence property of the carbon quantum dots, the Cr < 2 + > can quench the fluorescence of the selenium-doped carbon quantum dots, so that the Cr < 2 + > can be detected. Meanwhile, the carbon quantum dot is found to be responsive to disease micromolecule glutathione, that is, glutathione can enhance fluorescence of the carbon quantum dot. By utilizing the concentration difference of glutathione in normal cells and cancer cells, the carbon quantum dots can realize the recognition of tumor cells. The orange fluorescent carbon quantum dot provided by the invention is prepared from easily available raw materials, and is safe and non-toxic. The prepared carbon quantum dot has stable fluorescent property, uniform particle size of 4.2 + / -0.5 nm, and relative quantum yield of 34.35%, can be imaged in cells, has GSH detection limit of 27.16 umol / L, and can identify tumor cells and normal cells.

The invention relates to the technical field of microalgae biology, in particular to a chlamydomonas reinhardtii strain with high yield of exosomes, which is named as Chlamydomonas reinhardtii YYAW026 and preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.46629, the preservation date is November 10, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain, the chlamydomonas reinhardtii cell provided by the invention cannot form a complete cell wall structure, so that the exosome can be quickly secreted into a culture solution; although the cell structure of the strain is incomplete, the strain can still perform normal cell activity, the growth curve is basically consistent with that of a wild type, the yield of the exosome of the strain is greater than that of the wild type when the strain reaches a plateau phase, and the particle size distribution width of the exosome is more concentrated. The chlamydomonas reinhardtii disclosed by the invention has a relatively good application prospect in the basic research fields of cosmetics, medical beauty industry and algae source exosomes.

The invention discloses an enzyme-activated fluorescent molecular precursor as well as a preparation method and application thereof. The enzyme-activated fluorescent molecular precursor has a chemical structure as shown in a formula I which is described in the specification. The invention develops an enzyme-activated fluorescent molecular precursor which can be used for real-time imaging of tumors by targeting exogenous enzyme to the tumors and specifically activating a probe at the tumors; according to the invention, the exogenous enzyme is introduced as an activation trigger, and normal cells do not have the exogenous enzyme, so that the problem of co-expression of the endogenous enzyme in non-tumor tissues can be avoided, background fluorescence signals can be reduced to the greatest extent, and the imaging specificity and accuracy are remarkably improved.

A memory device includes a memory cell array including normal cells and per row activation count (PRAC) cells coupled with a plurality of word lines corresponding to a plurality of rows, a refresh control circuit configured to refresh the plurality of word lines corresponding to the plurality of rows based on a refresh command, and an activation generating circuit configured to generate a row activation signal based on an activation command. The row activation signal is configured to activate the plurality of word lines corresponding to a row address of the plurality of rows. Each of the PRAC cells is configured to store row activation information. Each of the normal cells is configured to store data.

The invention discloses an immortalized rabbit liver cell line as well as a construction method and application thereof, and the construction method is characterized in that an exogenous oTERT gene is introduced into rabbit embryo liver cells, so that the rabbit embryo liver cell line is immortalized. The successfully immortalized rabbit embryo liver cell line can be stably subcultured for more than 10 generations, and does not have an aging phenomenon. Compared with a traditional method (introducing exogenous immortalized genes such as cancer suppressor genes p53 and SV40TAg into cells), the TERT-mediated cells belong to normal cells, the growth characteristics of the TERT-mediated cells are kept consistent with those of the normal cells, the TERT-mediated cells belong to immortalization in a real sense, and a scientific and stable cell material can be provided for subsequent RHDV research. The construction method is simple, easy to implement and easy to popularize.

The present invention relates to a preparation method of a manganese tetraoxide / GSDME complex and its application, which can effectively solve the problem of tumor cell re-proliferation and normal tissue damage after radiotherapy. The technical solution is to firstly prepare a manganese tetraoxide / GSDME complex in the presence of Mn 3 O 4 The surface of the nanoparticles is modified with GSDME protein, and a manganese tetraoxide / GSDME complex is prepared by cross-linking tannic acid with the GSDME protein. The preparation method of the invention is stable and reliable, and is easy to operate. 3 O 4 The / GSDME complex has the advantages of good biocompatibility and low toxicity and side effects. It can achieve efficient intracellular delivery of GSDME protein, activate the Caspase-3 / GSDME signaling pathway, convert radiotherapy-induced tumor cell apoptosis into pyroptosis to inhibit tumor regeneration; and utilize the difference in intracellular GSH content between tumor cells and normal cells to inhibit the expression of Mn in tumor cells. 3 O 4 It can enhance radiosensitization by downregulating GSH, while Mn 3 O 4 It is an innovation in tumor radiotherapy drugs to exert its nanozyme activity to eliminate ROS produced by radiotherapy and achieve radiation protection for normal tissues.