90 results about "Dividing cell" patented technology

The invention discloses a preparation technology of a special cistanche inoculation block with a seed dormancy breaking self-induction function by combining a cistanche seed dormancy breaking inducer and hydrogel, and relates to the technical field of cistanche cultivation. The specific technical process is as follows: preparation of a dormancy breaking inducer solution, preparation of a hydrogel block containing the dormancy breaking inducer and cistanche seeds, and preparation of an inoculation block. According to the method, gibberellin and cytokinin are used as dormancy breaking inducers, and a yeast aqueous extract is used as an auxiliary agent. The problems of seed dormancy breaking, germination promoting and water retention inoculation are effectively solved, seed dormancy breaking does not need to be conducted in advance before inoculation, meanwhile, hydrogel has high water absorption and water retention capacity, the problem that dormancy breaking seeds are inactivated due to the fact that the dormancy breaking seeds cannot germinate and parasitize in time after being inoculated due to water shortage is effectively solved, and the survival rate of the seeds is increased. The inoculation efficiency is improved to 75% or above from about 20% of existing inoculation paper inoculation, and the method has a good popularization prospect and has very important significance in promoting the industrial development of cistanche deserticola.

The invention concerns methods for predicting changes in a tissue, comprising obtaining snap shot information on individual cells of at least one cell type in a section of a tissue biopsy, wherein the information comprises: (i) spatial localization, (ii) cell type and (iii) cell division state at a specific point in time; calculating cell division probabilities for each of said individual cells; determining cell population dynamics based on the cell division probabilities and predicting future changes in the tissue based thereon. Particularly, the method concerns predicting tissue dynamics in cancer biopsies relevant for prognosis, and assessment of response to therapy.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

Systems and methods for destroying or inhibiting cancer cells and other rapidly-dividing cells including an alternating polarity (AP) magnetic field generator and one or more AP electromagnetic coil adapted to be coupled to at least a portion of a patient's body, and a controller to control the AP magnetic field generator and at least one AP electromagnetic coil and cause the field generator and coil to apply AP magnetic field having a frequency of 0.5-500 KHz and a field strength of 0.5-5 mT to the at least a portion of the patient's body area to achieve a desired inhibiting effect on cancer cells or other rapidly-dividing cells. Treatments provided by the system may be co-administered with an anti-cancer therapy such as a chemotherapy drug, a hormone therapy drug, targeted therapy drugs, immunotherapy drugs, an angiogenesis inhibitor drug, or tumor treatment field therapy.

Described herein are devices for applying an alternating electric field to a living subject or an in vitro medium at a frequency between 100 kHz and 500 kHz. Also described herein are methods of using the described devices for applying an AC electric field to a target region comprising rapidly dividing cells, e.g., cells associated with a variety of disorders or conditions. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The disclosure relates to compositions and methods for enhancing asymmetric division of satellite cells and promoting muscle cell / tissue regeneration, for treatment of muscle tissue injuries and muscle diseases, including muscular dystrophies.

The application provides an analysis method, device and equipment based on single-cell transcriptome sequencing data, which comprises the following steps: performing quality control, downstream analysis and visual display on single-cell transcriptome sequencing expression quantitative data, performing cell filtering by using Grubbs test method to obtain effective single-cell transcriptome sequencing quantitative data, performing initialization clustering analysis on the data to obtain single-cell subgroup classification results; performing screening on the single-cell subgroup classification results to obtain target single-cell subgroups, and performing re-clustering analysis to obtain single-cell sub-subgroup classification results; performing significant difference gene screening analysis on the single-cell transcriptome sequencing quantitative data between single-cell subgroups; and performing regression analysis based on characteristic genes of cell cycles to predict cell division periods corresponding to different cell types. The application effectively solves the technical complex problems of existing single-cell transcriptome sequencing quantitative data analysis, makes data analysis more simple and reliable, and reduces the difficulty of data analysis.

The invention relates to compounds which inhibit Cyclin-Dependent Kinases such as CDK2 (Cyclin-Dependent Kinase 2 or Cell Division protein Kinase 2) and CDK4 (Cyclin-Dependent Kinase 4 or Cell Division protein Kinase 4), and to processes for the preparation of said compounds, pharmaceutical compositions comprising said compounds, and use of said compounds in the treatment of conditions, diseases and disorders mediated by CDK2 and / or CDK4.

The embodiment of the present application provides a kind of semiconductor integrated device, including cell, the shape of the cell is positive K polygon, wherein, the value of K is 4 or 6 or 8;The diagonal of the cell divides cell into K positive triangle structure, and one positive triangle structure contains one integrated HJD MOSFET or one integrated SBD MOSFET;Integrated HJD MOSFET is n, and integrated SBD MOSFET is m;Wherein, n and m are positive integer and the sum of n and m is K.The embodiment of the present application solves the technical problem that the reliability and turn-on capability of the conventional integrated schottky diode device are limited.

The invention discloses an identification method, device and system for a high gene module scoring area, and relates to the technical field of space transcriptomics. The identification method comprises the following steps: acquiring space transcriptome data, dividing cells into a plurality of unit grids according to space coordinate information of the cells, and determining corresponding grid space coordinates for each unit grid; calculating a module score value of each cell and determining a high-score cell set; calculating an integrated gene set scoring index; and carrying out region division on the unit grid and determining a high gene module scoring region. According to the method, a plurality of genes are analyzed as a functional whole, the limitation that traditional single-gene analysis cannot reveal collaborative regulation is overcome, spatial quantization of a gene set synergistic effect is realized, and the problem that a gene set enrichment region cannot be positioned in the prior art is solved.

The purpose of the present invention is to provide a fuel cell stack which has high output and high reliability by excluding divided cells that adversely affect the power generation output in cases where divided cells are constituted by dividing a fuel cell on a substrate. A fuel cell according to the present invention comprises a porous substrate, a first electrode layer, a solid electrolyte layer, and a second electrode layer. At least one of the first electrode layer and the second electrode layer is divided so as to form divided cells, and at least some of the divided cells each have an insulating buffer part that insulates the electrode layer from a collector (see Fig. 6).

The invention provides a physical experiment simulation system, and relates to the technical field of material mechanics tests, the physical experiment simulation system is based on an actual stress-strain curve, a simulation test piece three-dimensional model with a gap is established, cells are divided, a typical plane is selected, a groove cell, a slope bottom cell, a stretching cell and a gap cell are divided, and the physical experiment simulation system is obtained. The method comprises the following steps of: performing accurate simulation analysis on each position conveniently, directly simulating a real stretching effect in a stretching cell, acquiring radial stress at a groove cell according to a rotation trend generated by an intermolecular acting force, judging whether a notch cell and a slope bottom cell which serve as stress concentration points are in an elastic deformation stage or not through a vonMises yield criterion, and judging whether the notch cell and the slope bottom cell are in an elastic deformation stage or not. And the deformation condition of each cell caused by the influence of the stress is visualized, and the process and the result of the abnormal test result caused by the gap are intuitively displayed.

The present invention relates to a method for the in vitro characterization and quantification of proliferative cells in an epidermal fragment, comprising the following steps: 1) Culturing the epidermal fragment on a support, 2) Detaching the epidermal fragment from the support, 3) Performing immunostaining on the epidermal fragment, 4) Acquiring images of the basal layer of the epidermal fragment over its entire surface, 5) Reconstructing an image representing the entire basal layer of the epidermal fragment, 6) Selecting the immunostained nuclei of the proliferative cells by image processing, 7) Quantifying cell division by counting the nuclei through image analysis. The present invention also relates to using the method to evaluate the effect of a product or active ingredient on epidermal cell regeneration. Figure 1

The invention provides a fibroblast storage device and method, and relates to the technical field of microbiological devices.The device comprises a monitoring module, a control module and a storage module, the storage prediction module performs cell stage division according to the storage label and the real-time cell state data, establishes stage constraints, performs cell storage environment prediction by using the stage constraints and the real-time cell state data, and establishes a target prediction result; the environment detection module performs real-time acquisition of a storage environment of fibroblasts and establishes a storage environment data set; and the intelligent adjustment module takes the storage environment data set as an environment starting point, takes the target prediction result as a following target, executes search optimization of environment control under the constraint of the adjustment granularity identifier, and performs intelligent adjustment of the storage environment according to a search optimization result. The technical problem that the fibroblast storage reliability is poor in the prior art can be solved, and the technical effect of improving the storage reliability is achieved.

Described herein are devices for applying an alternating electric field to a living subject or an in vitro medium at a frequency between 100 kHz and 500 kHz. Also described herein are methods of using the described devices for applying an AC electric field to a target region comprising rapidly dividing cells, e.g., cells associated with a variety of disorders or conditions. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Provided are a method for producing a target molecule on the basis of a condensation-segregation technique, a recombinant strain and the use thereof. By means of regulating the formation of biological condensates, high-concentration enrichment of target molecules at the cell end is achieved. By means of this step, highly-enriched target molecules are obtained and can be further accumulated by means of cell division. Compared with traditional methods, the method not only effectively improves the concentration of target molecules in a specific region within cells, but also realizes further accumulation of the enriched molecules by means of the mechanism of cell division.

The application provides a kind of marine structure flow field simulation method, the method comprises: generating calculation grid and object surface grid for flow field simulation;Initialize object surface grid level set function field;According to flow field information, calculate the force condition of object surface, calculate object translation vector and rotation vector;Complete data mapping of object surface grid to calculation grid, obtain the level set function field of calculation grid;According to the obtained calculation grid level set function field, divide cell type;Carry out geometric calculation to cut cell, based on cut cell flow field reconstruction strategy, complete fluid cell, cut cell estimated speed update;Solve pressure Poisson equation, obtain pressure field, pressure gradient, update velocity field according to the obtained pressure gradient, obtain the final velocity field satisfying continuity condition.The marine structure flow field simulation method of the application greatly improves the numerical simulation efficiency of complex structure flow field, and reduces the implementation difficulty.

The invention provides a preparation method of a dimer amino acid compound. The preparation method comprises the following steps: providing L-serine, an indole derivative, Tm9D8 * protein, PmLAAD protein and dimer synthetase, and culturing the L-serine, the indole derivative, the Tm9D8 * protein, the PmLAAD protein and the dimer synthetase; wherein the dimer synthetase comprises an amino acid sequence which is shown as any one of SEQ ID NO: 1-4 or has at least 90% of identity with the dimer synthetase. The invention also provides a cell division inhibitor containing the dimeric amino acid compound, and a composition containing the dimeric amino acid compound or the cell division inhibitor.

The present disclosure provides methods for inducing cell cycle reentry of postmitotic cell. The present disclosure further provides cells and compositions for treating diseases, such as cardiovascular diseases, neural disorders, hearing loss, and diabetes.

The application provides a traffic cell division method based on resident travel characteristics. Administrative division boundaries and hierarchical road network vector data are acquired and data preprocessing is performed; basic clustering units are divided based on the road network and the administrative division; a clustering index system is established, and resident travel characteristics in the units are acquired through mobile phone signaling in combination with the clustering units; according to the location of the clustering units in the administrative division, a k-means clustering algorithm with spatial characteristics is adopted for preliminary clustering, and clustering results and clustering centers corresponding to each clustering number are obtained; clustering effectiveness indexes of the clustering results of each clustering number are calculated; the clustering effectiveness indexes of each clustering number are compared to determine a proper clustering number; final clustering division is performed through a FCM clustering algorithm with comprehensive spatial characteristics; the clustering results are modified in combination with main hierarchical roads, and traffic cell division is completed. The application improves the robustness of the traffic cell division results and can support the construction of subsequent traffic planning models.

The application discloses a manhole cover hidden danger integrated recognition method and system fusing target detection and segmentation, which firstly carries out pretreatment on manhole cover picture data, and divides cells and anchor frames.Secondly, multi-scale features of the pretreated manhole cover picture are extracted through YOLO v8x, and a manhole cover prediction result is outputted.A Bagging integrated learning strategy is adopted, and based on the manhole cover prediction result, a weighted voting mechanism is used to obtain a discrimination result of a manhole cover hidden danger category.Then, the pretreated manhole cover picture is inputted into an encoder module composed of PVTv2-B4 to extract manhole ring features, and a manhole ring prediction result is outputted.Meanwhile, based on the manhole ring prediction result, the manhole ring is analyzed again to obtain a discrimination result of a manhole ring hidden danger category.Finally, the discrimination results of the manhole cover and the manhole ring are combined to obtain a hidden danger category.The application improves the fine recognition ability of the manhole cover state in a complex scene, and realizes accurate outlining and positioning of the manhole ring boundary.

The invention particularly relates to the technical field of stem cell preparation and tissue engineering, and discloses a placenta mesenchymal stem cell multi-source extraction system based on an integrated treatment platform. Comprising a multi-region synchronous pretreatment module, a zoning adherent culture module, a differentiated cell harvesting module, a cell characteristic collection module, a functional cell division module and a whole-process closed-loop control module, the multi-region synchronous pretreatment module adopts an integrated design, and the zoning adherent culture module realizes centralized culture through an independent marking region; the differentiation cell harvesting module is used for carrying out merging amplification; the cell characteristic acquisition module is used for acquiring cell characteristic data; the functional cell division module is used for realizing cell type and function judgment; the whole-process closed-loop control module is used for realizing whole-process quality tracing; through the integrated design, the cost is reduced, the cell quality stability is ensured, and the whole-process quality tracing is realized.

The present invention provides a culture medium for amplifying hematopoietic cells having cell division ability, the culture medium containing a compound (I) represented by formula (1) or a physiologically acceptable salt thereof: in the formula (1), R1, R2, and R3 are each independently any one of hydrogen, a linear or branched C1-C4 alkyl group, bromine, and iodine, R4 is any one of a linear or branched C1-C4 alkyl group, bromine, and iodine, and R1, R2, and R3 are each independently any one of hydrogen, linear or branched C1-C4 alkyl groups, bromine, and iodine. R5 is any one of oxygen, methylene and a chemical bond, R6 is hydrogen or-NH2, R7 is hydrogen or-COOH, and R6 and R7 are not hydrogen at the same time.

The present invention relates to a method of generating an in vitro modular 3D brain tissue model (3D BTM) comprising: a. mixing 50,000 to 1,000,000 freshly splitted, single neurons (NE) and astrocytes (AS) or their precursor cells in a ratio of NE:AS = 1:3 to 10:1, followed by spinning down to introduce self-aggregation in low attachment conditions, without addition of exogenous matrices; b. treating cells obtained in step a. with a NOTCH signaling inhibitor for driving terminal neuron differentiation for at least 2 days; c. treating the cells obtained in step b. with at least one mitotic inhibitor to remove residual dividing cells for at least 3 days, thereby yielding a post-mitotic aggregated 3D culture of neurons and astrocytes; d. adding differentiated microglia (MG) to the culture obtained in step c. in a ratio of NE:MG = 1:1 to 20:1, to initiate migration of MG into the culture for 3-10 days; e. culturing the culture obtained in step d. in neuron media with addition of TGFβ1 and cytokines selected from the group comprising CSF1-R agonists to support microglia maintenance and proliferation for at least 2 weeks, thereby inducing formation of a modular in vitro 3D BTM culture.

The application discloses a kind of urban high-density road network mixed traffic flow cell transmission simulation prediction methods, comprising: with urban high-density road network area as modeling object, with road boundary end as traffic flow input position and output position, the road section between input position and output position includes urban expressway basic road section, access ramp and interchange, and urban non-expressway basic road section and intersection, the road section between input position and output position is divided into cell, and mixed mixed traffic flow cell transmission model is established to the cell, and the state of urban road network is simulated and predicted.The application comprehensively considers the traffic flow of urban expressway and urban non-expressway, effectively connects the cell of urban expressway with the cell of urban non-expressway, and designs and improves the cell division and modeling method of interweaving area and intersection, which is beneficial to the comprehensive study of traffic flow change in urban road network area and the traffic flow state simulation prediction for traffic flow control.

The invention discloses trophoblasts for amplification of clinical-grade tumor infiltrating lymphocytes and a preparation method and application of the trophoblasts, and the preparation method comprises the following steps: 1) placing tumor tissues in a complete culture medium, placing the complete culture medium in a 5% CO2 incubator at 37 DEG C, and carrying out wet culture for 2-3 weeks; after finishing, sorting the lymphocytes by magnetic beads; (2) adding the lymphocytes into an amplification culture medium for culturing, transferring the lymphocytes into a cell culture bag after the total number of the cells is greater than 107-108, culturing until the total number of the cells reaches 109-1011, and centrifugally collecting the cells to obtain tumor infiltrating lymphocytes TIL; 3, 10-50 Gy X rays or gamma rays are used for irradiating the tumor infiltrating lymphocytes to damage DNA of the tumor infiltrating lymphocytes to prevent cell division, and the trophoblasts are obtained.According to the method, a large number of amplified TIL is used for replacing PBMC to serve as the trophoblasts for immune cell proliferation, and the lymphocyte trophoblasts which are easier to obtain and better in nourishing effect are provided.

The invention relates to the technical field of growth regulators, and particularly discloses a plant growth regulator containing alkenyladenine and oxyenyladenine and application of the plant growth regulator in crop stress resistance. The plant growth regulator is prepared from 0.000125% of alkenyladenine, 0.000875% of oxyenyladenine, 1 to 10% of surfactant, 0.1 to 0.5% of stabilizer, 2 to 5% of cosolvent, 0.1 to 0.5% of defoaming agent, 0.1 to 0.5% of synergist, 0.01 to 0.1% of pH regulator, 0.01 to 0.05% of preservative and the balance of water. Compared with the prior art, the prepared plant growth regulator is more stable in property and not prone to deterioration and precipitation. By compounding alkenyladenine and oxyenyladenine, cell division is synergistically promoted, cold resistance, drought resistance and other capacities are enhanced, and growth quality improvement, stress resistance and disaster reduction are both considered.

The present disclosure relates to compounds of the formulae herein (e.g, Formula (II)), and pharmaceutically acceptable salts, solvates, hydrates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled compounds, or prodrugs thereof, which are inhibitors of a GTPase (e.g., cell division control protein 42 homolog (Cdc42)). The present disclosure also provides pharmaceutical compositions comprising the compounds, pharmaceutically acceptable salts, solvates, hydrates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled compounds, or prodrugs thereof, and methods of treating diseases (e.g., malignant neoplastic diseases, diseases associated with aging) by administering to a subject in need thereof the compounds, pharmaceutically acceptable salts, solvates, hydrates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled compounds, or prodrugs thereof.

This disclosure provides a dimer amino acid compound, a cell division inhibitor comprising the dimer amino acid compound, and a composition comprising the dimer amino acid compound or the cell division inhibitor.

The invention relates to the field of fasteners, in particular to a method and a system for simulating impact resistance of a fastener in a low-temperature environment. The method comprises the steps of performing coarse cell division on a constructed fastener three-dimensional model to obtain cells, and calculating a global thermal stress gradient mean value and a global impact stress gradient mean value of all the cells; calculating a stress distance between any two cells to obtain an evaluation distance, clustering all the cells, and updating the side length of the cells in a cluster to obtain an updated side length; calculating the surface gradient tensor of the mark lattice, calculating the stress dominant weight of any surface of the mark lattice according to the surface gradient tensor, weighting the normal form equivalent stress to obtain adaptive normal form stress, constructing a model, and completing the evaluation of the low-temperature impact resistance of the fastener. According to the technical scheme, the precision of the impact resistance evaluation result of the fastener can be improved.