403 results about "Dividing cell" patented technology

An apparatus is provided for selectively destroying dividing cells in living tissue formed of dividing cells and non-dividing cells. The dividing cells contain polarizable intracellular members and during late anaphase or telophase, the dividing cells are connected to one another by a cleavage furrow. The apparatus includes insulated electrodes to be coupled to a generator for subjecting the living tissue to electric field conditions sufficient to cause movement of the polarizable intracellular members toward the cleavage furrow in response to a non-homogeneous electric field being induced in the dividing cells. The movement of the polarizable intracellular intracellular members towards the cleavage furrow causes the breakdown thereof which adversely impacts the multiplication of the dividing cells. Preferably, an intervening member is disposed between each insulated electrode and the skin surface and each intervening member includes a conductive “floating” plate that protects against the effects on the patient from a breakdown in the insulation of the electrode.

The present invention provides a method and apparatus for selectively destroying dividing cells in living tissue formed of dividing cells and non-dividing cells. The dividing cells contain polarizable intracellular members and during late anaphase or telophase, the dividing cells are connected to one another by a cleavage furrow. According to the present method the living tissue is subjected to electric field conditions sufficient to cause movement of the polarizable intracellular members toward the cleavage furrow in response to a non-homogenous electric field being induced in the dividing cells. The non-homogenous electric field produces an increased density electric field in the region of the cleavage furrow. The movement of the polarizable intracellular members towards the cleavage furrow causes the break down thereof which results in destruction of the dividing cells, while the non-dividing cells of the living tissue remain intact.

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol*I−1*pH−1 and an ionic strength of at least 200 mmol*I−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution.

A vector capable of transducing non-dividing and / or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the protein of interest in the cell. In addition, target cells containing the lentiviral LTR-deleted vector are provided.

The erasion level appending range of a file is instructed. According to this instruction, the erasion level is set. When this setting range does not agree with the cell unit of the current cell, the current cell is divided to set a sequence of divided cells. Playback management information of each of the divided cells and current cell is re-created, and erasion level information is appended to playback management information of the divided cell that belongs to the appending range.

Methods and compositions for modulating the FGF effect on the sensitivity of malignant and normal cells to anticancer agents are provided. In particular, methods and compositions for inhibiting FGF-induced resistance to a broad spectrum of anticancer agents in solid and soft-tissue tumors, metastatic lesions, leukemia and lymphoma are provided. Preferably, the compositions include at least one FGF inhibitor in combination with a cytotoxic agents, e.g., antimicrotubule agents, topoisomerase I inhibitors, topoisomerase II inhibitors, antimetabolites, mitotic inhibitors, alkylating agents, intercalating agents, agents capable of interfering with a signal transduction pathway (e.g., g., a protein kinase C inhibitor, e.g., an anti-hormone, e.g., an antibody against growth factor receptors), an agent that promotes apoptosis and / or necrosis, and interferon, an interleukin, a tumor necrosis factor, and radiation. In other embodiments, methods and composition for protecting a cell in a subject, from one or more of killing, inhibition of growth or division or other damage caused, e.g., by a cytotoxic agent, are provided. Preferably, the method includes: administering, to the subject, an effective amount of at least one FGF agonist, thereby treating the cell, e.g., protecting or reducing the damage to the dividing cell from said cytotoxic agent.

The present invention is related to methods in which an electric field pulse is applied to cells and tissue. Several embodiments of the present invention relate to the application of electric field pulses to cells to regulate the physiology and biophysical properties of various cell types, including terminally differentiated and rapidly dividing cells. Methods of regulating transcription of a gene in a cell, marking a cell for diagnostic or therapeutic procedures, determining cellular tolerance to electroperturbation, selectively electroperturbing a population of cells, reducing proliferation of rapidly dividing cells in a patient, and facilitating entry of a diagnostic or therapeutic agent into a cell's intracellular structures are also provided.

The invention discloses a day-dimension regional traffic index prediction method considering influences of multiple factors. The method comprises the steps that regions are divided and aggregated; regional traffic index original data preprocessing is carried out; the influences of multiple factors are considered, and regional traffic index prediction under the day dimension is carried out. According to the specific technical scheme of the method, on the basis of traffic cell division, traffic cells with the same aggregation property are aggregated, and regional traffic indexes are calculated;on the basis of road network operation early warning requirements, a prediction time period and a prediction cycle are determined; regional traffic data is extracted, made up for and removed, and preprocessing such as comprehensive building of a historical data factor attribute set from different angles is conducted on the data; on the basis of a decision tree theory, regional road network operation congestion state prediction is carried out; a final prediction result of the regional traffic indexes is determined by means of the square euclidean distance. By means of the method, on the one hand, monitoring and application of the urban road network operation state is deepened, and on the other hand, technical support is provided for early warning and forecasting work of the road network operation state.

A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.

The invention discloses a cell division detection method based on deep learning, relates to the technical field including cytobiology and the like, and solves the problem of inaccurate cell division detection results. The cell division detection method comprises the following steps: obtaining an image in a cell culture process, and forming a continuous image sequence according to the images obtained at certain time intervals; judging and comparing all cell division processes in the image sequence, and labelling a position where a process that a paternal cell is divided into two daughter cell membranes can be clearly observed in cell division, and forming a labelled dataset; constructing a full convolutional neural network model, determining the parameter of the full convolutional neural network model, loading the labelled dataset into the full convolutional neural network model, using a deep learning method to directly carry out feature learning on an image sequence, and obtaining the trained full convolutional neural network model; and deploying the trained full convolutional neural network model for automatically detecting the cell division of the cell culture data of a similar type. The cell division detection method is used for cell division detection.

The invention provides methods, reagents and systems to preferentially mark fast-proliferating cells / tissues (such as cancer), by incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to for a number of uses, such as imaging of cancer cells, targeting drugs to preferentially kill cancer cells, and proteomic analysis in the context of large scale or high throughput screening for candidate drug leads that affects the proliferation of a target cell, etc.

A method including: a dividing step (A) of dividing external data into a plurality of cells (13) having boundaries orthogonal to each other, the external data including boundary data of an object which contacts incompressible viscous fluid; a cell classifying step (B) of classifying the divided cells into an internal cell (13a) positioned inside or outside the object and a boundary cell (13b) including the boundary data; a cut point determining step (C) of determining cut points in ridges of the boundary cell on the basis of the boundary data; a boundary face determining step (D) of determining a polygon connecting the cut points to be cell internal data for the boundary face; and a analyzing step (E) of applying a cut cell finite volume method combined with a VOF method to a boundary of a flow field to analyze the flow field.

Disclosed herewith is a multi-plane cell switch fabric system in which each switching unit functions asynchronously with others. The system executes distribution / restoration operations without lowering the switching capacity to reorder cells that arrive in random order from a plurality of switches just as they were in original flows and packets respectively with a small hardware capacity. In the system, the distribution unit divides each variable length packet addressed to the same destination into fixed length cells and sends those divided cells by a unit of integer multiple of the number of switches. On the other hand, the reordering unit, while holding cells that arrive in random order from each switching unit (switching units 1 to M) in a receive buffer, separates only the header information from each cell and holds the header information in a retry queue. The reordering unit, upon finding the header information of a head cell of a flow in an ordering check at the time of receiving or in a retry check in the retry queue, extracts the corresponding cell bodies from the receive buffer to restore the original order of those cells in the subject packet.

The invention discloses a regional rail network UAV cruise method based on dynamic cell division. The method comprises the following steps; grading roads, dividing all the roads into important parts and unimportant parts according to grades so that there are differences when the cruise is arranged; carrying out preliminary flight cell division to the whole area according to a UAV cruise radius and a ground control center, wherein one UAV cruises one flight cell; carrying out UAV flight path optimization to the roads with different grades in the each flight cell and performing cruise time constraint examination according to a UAV path optimization result; if a condition is satisfied, determining the path as the optimal path; otherwise, turning into a next step; if the cruise time constraint is not satisfied, using a cluster analysis method to further dividing the each cell; repeating the above steps till all the constraints are satisfied so as to obtain the final optimization cell and the optimization path and determine a cruise scheme.

A method including: a dividing step (A) of dividing external data into a plurality of cells (13) having boundaries orthogonal to each other, the external data including boundary data of an object which contacts incompressible viscous fluid; a cell classifying step (B) of classifying the divided cells into an internal cell (13a) positioned inside or outside the object and a boundary cell (13b) including the boundary data; a cut point determining step (C) of determining cut points in ridges of the boundary cell on the basis of the boundary data; a boundary face determining step (D) of determining a polygon connecting the cut points to be cell internal data for the boundary face; and a analyzing step (E) of applying a cut cell finite volume method combined with a VOF method to a boundary of a flow field to analyze the flow field.

The present invention relates to a cell division inhibitor comprising various dehydrodiketopiperazines such as dehydrophenylahistin, or analogs thereof as an active ingredient, and a dehydrogenase and a method for producing the same inhibitor.

This invention discloses the use of an E-selectin antagonist and a mobilizer of hematopoietic stem cells or progenitor cells in methods and compositions for treating or preventing immunocompromised conditions resulting from medical treatment. The present invention is particular relevant for prophylaxis and / or treatment of hematopoeitic disorders including neutropenia, agranulocytosis, anemia and thrombocytopenia in individuals receiving or proposed to receive treatments that target rapidly dividing cells or that disrupt the cell cycle or cell division.

The invention discloses an automatic detection method of abnormal cervical cells based on a depth convolution neural network, which mainly comprises the following steps: (1) manual labeling of a TCT cervical cell picture; (2) segmentation of labeled cell images; (3) multi-classification of cells after segmentation; (4) training of cell images in migration model; (5) segmentation, recognition and localization of the pictures of the cells to be detected; (6) re-segmentation and recognition of abnormal cell clusters. The automatic detection method for abnormal cervical cells of the invention classifies the divided cells into nine types, trains the cells by migration learning mode, and obtains a better fitting multi-classification model. The model can identify, screen and accurately locate theunlabeled source cervical cell images, and re-segment and identify the abnormal cells, which improves the detection accuracy. The invention has good auxiliary effect in the field of cervical cell pathological diagnosis.

A system and method for modeling a geological structure may include, in an initial model, computing a first function for a geological structure including a first set of iso-surfaces. A processor may detect if the first set of iso-surfaces intersect a set of geological markers within a threshold proximity. If not, the initial model may be corrected using an induced mesh having an increased cell resolution compare to the initial model for computing a second function for the geological structure including a second set of iso-surfaces that intersect the geological markers within the threshold proximity. A processor may insert the second set of iso-surfaces into a second model to locally increase its resolution relative to the initial model by dividing cells in the second model along the second set of iso-surfaces. For each new geological structure, the above steps may be repeated using the second model as the initial model.

The invention belongs to the technical field of road design, and relates to a method for determining the minimum distance of road intersections in a harbor district. In the method, frequent lane-changing area of harbor district roads connected with the intersections and the intersections are subjected to two-dimensional cell division, and other parts are one-dimensional cells, and a cell model for harbor district roads near the intersections is constructed. The method comprises the following steps of: (1) constructing a driver sensing module; (2) constructing a behavior module; and (3) setting appearing ratios of various vehicles in a main road, speeds of the various vehicles, and departure frequencies of various vehicles, performing simulation experiments under the conditions of different distances of the intersections and different departure frequencies, and analyzing the simulation to obtain a traffic flow space-time map and vehicle delay data so as to obtain the minimum distance of proper intersections under different traffic conditions. By the method, the influence of the distance of the intersections on the road capacity of the harbor district can be accurately reflected, sothat a method for determining the distance of the road intersections in the harbor district under different conditions is provided.

The present invention provides an electronic terminal having a screen division display function, the terminal including a memory for storing at least one document file and a viewer program for opening and showing the contents of the document file; and a control unit for executing the viewer program to divide a page of the document file stored in the memory into a plurality of cell, display one of the divided cells in one area of a screen window and display one or more cells adjacent to the cell displayed in the one area of the screen window in other areas of the screen window. According to the present invention, movement among the divided cells can be easily implemented using the direction keys provided on the electronic terminal such that the number of movements of a cursor required for displaying the entire document can be drastically decreased even when a document is enlarged such that it may be recognized with the naked eye.

The present invention relates to retroviral vectors which will infect and confer efficient gene transfer to non-dividing cells including the cells of the central nervous system. The vector system of the present invention is useful as a gene transfer vehicle for gene therapy, i.e. of the central nervous system.

The invention discloses a method for differential counting of white blood cells based on morphology. The method includes the steps that after Wright stain is conducted on peripheral blood smears, a microscope is used for collecting an image; after the image is processed by a computer system, morphology parameter analysis is conducted on the white blood cells, and the white blood cells are divided into a lymphocyte area, a monocyte area, a granulocyte area and a cell extinction area; the morphology parameter analysis is conducted on the area cells which are divided in the previous step, and the cells are further divided into monocytes, lymphocytes, eosinophilic granulocytes and neutrophile granulocytes precisely; the differential counting statistics are carried out on the variety of the divided cells. The computer system is used for analyzing the processed image, several simple morphology parameters are used for making a two-dimensional scatter diagram, all the cells are presented in a statistical diagram mode, the white blood cells can be classified rapidly and precisely, white blood cell counting is automatic, compared with manual counting, the procedures are simple and fast, the diagnose speed is greatly increased, and accuracy is improved substantially.