28 results about "Cell tracking" patented technology

This invention provides a long-afterglow compound, system, preparation method, and application based on covalent bond coupling. The long-afterglow compound absorbs photons and transfers the absorbed photon energy within the molecule, undergoing a photochemical reaction that releases the energy as light, thus achieving afterglow luminescence. The structure of the long-afterglow compound is shown in Formula I, where X is selected from O or S; R is selected from a photosensitizer; and n is 1–15. The long-afterglow system includes a carrier medium and a long-afterglow compound, wherein the carrier medium is used to dissolve, disperse, or adsorb the long-afterglow compound. This invention can achieve near-infrared luminescence at 660 nm and 718 nm, and can be applied in fields such as bioimaging, immune detection, cell tracking, biosensing, and information encryption.

According to exemplary embodiments, a cell tracking controller can be provided. The cell tracking controller may comprise: a cell ID collection unit for collecting a cell ID, which is identification information of a battery cell; a data collection unit for collecting at least one from among inspection data of the battery cell, measurement data of the battery cell, and equipment data collected from equipment of a battery cell manufacturing process or an equipment controller for controlling the equipment; and a cell ID mapping unit for mapping, to the cell ID, at least one from among the inspection data, the measurement data, and the equipment data corresponding to the cell ID, wherein the cell ID collection, the data collection and the data mapping can be performed in real time.

A memory apparatus includes memory cells connected to word lines and configured to store a threshold voltage corresponding to data states. The memory cells are disposed in memory holes coupled to bit lines. A control means applies program pulses to selected ones of the word lines. The control means determines when the threshold voltage of each of the memory cells exceeds a lower verify level of one of the data states targeted and maintains a respective count of a number of the program pulses subsequently applied thereto before reaching a lockout state. Each of the program pulses is divided into phases. The control means sets a voltage of one of the bit lines coupled to each of the memory cells at a stepped up level as a function of the respective count and according to each of the phases in which the voltage is set to the stepped up level.

A cell tracking method according to an embodiment of the present disclosure may comprise the steps of: detecting a semi-finished cell on a process line; generating a virtual ID of the semi-finished cell on the basis of the detection of the semi-finished cell; acquiring a cell ID of the semi-finished cell by detecting a code object formed on the semi-finished cell; and matching the virtual ID and the cell ID.

The present application relates to a suicide gene system and its use as part of immunotherapy. In some embodiments, the suicide gene system is expressed in a CAR T-cell, optionally along with armoring proteins. The present application also discloses uses of the suicide gene system, such as in cell tracking, immune regulation, rapid elimination of CAR T-cells, and as part of a therapeutic treatment.

The invention discloses a pathological section three-dimensional scanning system. The system comprises a cell pathological section double-plane full-automatic scanning subsystem, a double-plane reflection type digital holographic tomography module and a cell online real-time tracking analysis module. Wherein the cell pathological section double-plane full-automatic scanning subsystem is used for controlling synchronous imaging of the upper surface and the lower surface of a target section; the biplane reflection type digital holographic tomography module is used for performing multi-angle illumination on a target slice to obtain a multi-angle hologram, and further realizing unmarked three-dimensional imaging by adopting a three-dimensional reconstruction algorithm combining a physical model and a deep learning model to obtain a reconstructed high-resolution three-dimensional image; and the cell online real-time tracking analysis module realizes real-time analysis by using deep learning based on the intensity and transmission coefficient graph of the reconstructed high-resolution three-dimensional image, realizes colony area detection and cell type identification, and locates the spatial position of an abnormal cell. According to the invention, the overall efficiency and resolution of pathological section imaging are improved.

The invention discloses a cell tracking probe as well as a preparation method and application thereof. The retention time of the existing dye is relatively short (generally only several hours); the dye is not tolerant to fixation treatment, so that the dye leaks from cells, and the problem of efflux also exists; more seriously, the novel cell tracking and cell viability fluorescent dye has fluorescence, and aims to provide the novel cell tracking and cell viability fluorescent dye which is longer in retention time, does not need an additional cleaning step and is smaller in toxicity aiming at the technical problems existing in the existing main calcein orange red dye.

According to exemplary embodiments, a battery cell tester may be provided. The battery cell tester may be configured to: generate test data on the basis of a test signal of a battery cell; receive a cell ID of the battery cell; generate mapping data by mapping, to the cell ID, the test data corresponding to the cell ID; and transmit the mapping data to at least one upper server. According to exemplary embodiments, a cell tracking controller may be provided.

According to an exemplary embodiment, a cell tracking data management method is provided. The method comprises the following steps that battery cell tracking data including unidentified roll core IDs and roll core IDs adjacent to the unidentified roll core IDs are loaded, the roll core IDs are used for distinguishing roll cores, and the roll cores are of a winding structure of a positive electrode, a negative electrode and a diaphragm; and recovering the unidentified roll core ID based on the roll core ID.

A cell tracking method according to one embodiment of the present disclosure may comprise the steps of: acquiring, on the basis of a first sub-process included in a secondary battery manufacturing process, an electrode ID corresponding to a unit electrode; acquiring, on the basis of a second sub-process included in the secondary battery manufacturing process, a cell ID corresponding to a semi-finished cell including the unit electrode; and matching the electrode ID and the cell ID.

Compositions and methods are directed to a diverse library of barcoded constructs expressing biologies that are known to be challenging to express. A high throughput screening assay is also disclosed.

The invention discloses a sperm cell detection-based processing method and a storage medium, a microscope camera is used to shoot sperm to be detected at fixed time intervals, and an improved YOLO target detection model is used to carry out sperm cell detection, so that accurate detection of sperm cells is realized; according to the method, sperm cells are tracked by using a sperm cell tracking model, sperm cell information is recorded into a database, data support is provided for subsequent analysis, and a method for judging the motion trail and length of target sperm cells is established according to the sperm cell information database. According to the method, the number of the sperm frame images is set, the motion trail length corresponding to the number of the sperm frame images and the sperm cell activity degree judgment threshold value are set, the sperm activity degree can be objectively evaluated, a scientific basis is provided for sperm quality evaluation, the sperm quality can be efficiently and accurately evaluated, and important data support is provided for reproductive medicine research.

This invention relates to a method for the directional induction of iPSCs into notochord cells. By precisely simulating embryonic development signaling pathways and combining innovative cell tracking tools, it achieves precise navigation of the differentiation path from pluripotent state to notochord cells, thereby significantly improving the differentiation yield and purity of iNCs.

The invention relates to the technical field of image processing, in particular to an image processing method for dynamically monitoring cell proliferation. The method comprises the steps that an original microscopic image is acquired and preprocessed, a structure three-channel tensor is constructed based on the preprocessed microscopic image, a disturbance mechanism driven by structure response is introduced, and a disturbance tensor of the preprocessed microscopic image is obtained; projecting the disturbance tensor of the preprocessed microscopic image to a combined space-frequency domain to obtain a multi-scale frequency response; superposing the multi-scale frequency responses to obtain a space-frequency joint image response; and performing brightness compression and edge smoothing on the space-frequency combined image response to obtain a reconstructed microscopic image, and performing cell tracking and behavior recognition to realize dynamic monitoring of cell proliferation. The problems that in the cell proliferation dynamic monitoring process, image processing is not accurate, cell tracking is prone to being interrupted in a complex scene, and behavior recognition capacity lacks time sequence dynamic modeling capacity are solved.

According to exemplary embodiments, a battery manufacturing system may be provided. The system may comprise: a cell tracking controller configured to collect a cell ID, which is identification information of a battery cell; a data collection device configured to collect high-frequency data generated according to the progress of a process for the battery cell; and a mapping device configured to map the high-frequency data corresponding to the cell ID to the cell ID.

The application belongs to the technical field of cell tracking, and in particular discloses a cell tracking method based on deep learning and an ant colony algorithm, wherein multiple cells are accurately detected in a deep learning manner, double determination of the ant colony algorithm and Kalman filtering avoids target loss caused by irregular movement of cells, and accurate determination is made on cells with complex behaviors. The ant colony algorithm cost formula adopted by the application comprehensively considers the centroid information and the contour information, so that not only the centroid coordinate position is considered when interframe matching is performed, but also the cell morphological information is considered, and more accurate tracking effect is achieved when the cell has complex behaviors; the target detection method based on yolov7 has a good detection effect on similar targets of different sizes by using a mosaic enhanced data enhancement method, and the performance is not reduced due to adjustment of the microscope magnification; the method combining yolov7 and U-Net enhances the visibility of the system.

A cell tracking method for performing cell tracking on the basis of a plurality of cell images captured in time series, including an extraction process of extracting a tracking region corresponding to the cell for each cell images; a tracking process of calculating a change information in a position of the extracted tracking region and tracking the cell on the basis of the change information; a determination process of determining whether or not the cell as a tracking target is in a cell division state on the basis of the cell image; and an analysis process of analyzing a movement state of the cell on the basis of the change information from which the change information in a period of the cell division state is excluded when the cell is determined to be in the cell division state in the determination process.

A polynucleotide comprising a nucleotide sequence encoding an epidermal growth factor receptor (EGFR) extracellular epitope operably linked to: (a) an NGFR or EGFR SFR alpha signal peptide; (b) an EGFR or NGFR transmembrane domain; and / or (c) an NGFR or EGFR cytoplasmic tail.

The present application relates to the technical field of image processing, and more particularly to an image processing method for dynamic monitoring of cell proliferation. The content includes: obtaining an original microscopic image and performing preprocessing, constructing a structural three-channel tensor based on the preprocessed microscopic image, and introducing a disturbance mechanism driven by a structural response to obtain a disturbance tensor of the preprocessed microscopic image; projecting the disturbance tensor of the preprocessed microscopic image to a joint space-frequency domain to obtain a multi-scale frequency response; superimposing the multi-scale frequency response to obtain a space-frequency joint image response; performing brightness compression and edge smoothing on the space-frequency joint image response to obtain a reconstructed microscopic image, and performing cell tracking and behavior recognition to realize dynamic monitoring of cell proliferation. The problems of inaccurate image processing, easy interruption of cell tracking in complex scenarios, and lack of time sequence dynamic modeling ability in behavior recognition ability in the process of dynamic monitoring of cell proliferation are solved.

A cell tracking method, according to one embodiment of the present disclosure, may comprise the steps of: generating a virtual ID of a target semi-finished cell on a process line; acquiring process data corresponding to the target semi-finished cell; and mapping the virtual ID and the process data.

According to one embodiment of the invention, the battery cell tracking method comprises the following steps: acquiring a battery cell ID corresponding to a semi-finished battery cell; obtaining a retainer ID corresponding to a retainer for arranging the semi-finished battery cell; and matching the ID of the battery cell with the ID of the retainer.

The present application relates to a method for improving the accuracy of in vivo cell tracking using iron chelators, comprising the following steps: S1, preparing drug materials; S2, preparing animals and cells: preparing bone marrow cells, S3, removing free iron chelators in vivo 89 Zr and 68 Ga, S4, radiolabeling mesenchymal stem cells and bone marrow cells; S5, performing Micro-PET imaging: S6, performing Micro-PET imaging data processing and analysis: S7, in vitro biodistribution; S8, performing pharmacokinetic studies; S9, extracting hAlu genomic DNA and performing qPCR detection; S10, establishing a standard curve of cell number and cycle threshold; S11, performing absorbed dose estimation; S12, performing in vitro cell experiments; S13, final statistical analysis. The present application provides a method for improving the accuracy of in vivo cell tracking using iron chelators, which realizes the effect of removing free radioactive metal ions in vivo using iron chelators, and ensures the accuracy of in vivo tracking labeled cells.

A cell tracking method executed by a cell tracking system including a sensor subsystem (110,130) and a signal processing subsystem, the method comprising: detecting, by the sensor subsystem (110), a semifinished cell (306) on a process line (300); generating, by the signal processing subsystem, a virtual identification, ID, for the detected semifinished cell (306) upon detection of the semifinished cell (306); acquiring a cell ID of the semifinished cell (306) by detecting, by the sensor subsystem (130), a code object formed on the semifinished cell (306); and matching, by means of the signal processing subsystem, the virtual ID and the cell ID.

As an exemplary embodiment, a cell tracking controller may be provided. The cell tracking controller may include: a cell ID collection unit configured to collect a cell ID which is identification information about a battery cell; a data collection unit configured to collect at least one of inspection data of the battery cell, measurement data of the battery cell, or facility data of a battery cell manufacturing process; a cell ID mapping unit configured to map, to the cell ID, at least one of the inspection data, the measurement data, or the facility data corresponding to the cell ID; and a data conversion unit configured to convert binary data mapped to the cell ID into string data. According to exemplary embodiments, a cell tracking controller may be provided.

Methods for the development and integration of multiple apparatuses and methods for achieving administration of stem cell therapies include precision manufacturing of tissue scaffolds and / or bioreactor substrates. The nano / microscale fiber material extrusion typifying the electrospinning process is married with the fiber alignment and layering characteristic of an additive manufacturing process. The method generates porous fibrous 3-D meshes with precision controlled structures from biopolymer melts and solutions and gels, blends, and suspensions with and without cells. A method of tracking the migration histories and shapes of stem cells on scaffold surfaces relies on immunofluorescent imaging and automated algorithms based on machine learning. The combination of the precision manufacturing method and the method of cell tracking and cell shape statistics, along with understanding of the intimate relationship between the cell shape / phenotype and scaffold architecture leads to an integrated method for cultivating and harvesting cells having desired phenotypes.

This invention discloses a method and system for cell trajectory tracking repair and purification. The method includes: during frame-by-frame tracking, real-time logical interception of connection edges in the current frame is performed through an iterative closed loop including verification, elimination, and recalculation to generate a preliminary trajectory; for remaining trajectory breaks after tracking, a dual-track detection mechanism is used to locate candidate breaks, generate virtual repair patches, and perform pixel-level competitive segmentation of the spatial overlap area between the patches and existing cells to achieve physical trajectory completion; the generated preliminary trajectory is globally purified, including downgrading abnormal divisions, performing spatiotemporal source auditing on debris candidate targets for merging or elimination, and eliminating logically distorted branches through a recursive clearing operator, outputting the final lineage data. This invention solves the problem of low lineage construction quality caused by error accumulation, under-segmented broken links, and over-segmented debris, significantly improving the accuracy and biological consistency of cell tracking.