Preparation and application of double-fluorescent polymer quantum dot material

Abstract

The invention discloses a double-fluorescent polymer quantum dot material. A hydrophobic TPE blue chromophore with an AIE (Aggregation-Induced Emission) property and amphiphilic PNIPAM (Poly(N-isopropylacrylamide)) are covalently connected together to self-assemble a polymer quantum dot TPE-monoPNIPAM with a nano-scale; then the polymer quantum dot is coordinated with Eu(III) to prepare TPE-monoPNIPAM-Eu(III); the polymer quantum dot has very good double-fluorescent property, aggregation-induced emission property and temperature-sensitive property. A Hela cell staining result shows that the polymer quantum dot can be used for staining cytoplasm very well and is aggregated in the cytoplasm so as to generate high-brightness fluorescent light; blue and red composite light can be generated under different excitation wavelengths; especially, bright blue light can be generated under the excitation of light with the wavelength of 365nm and high-brightness red light is generated under the excitation of light with the wavelength of 395nm; the double-fluorescent polymer quantum dot material can be applied to the fields of biology better, such as cell imaging and cell tracking.

Description

technical field [0001] The present invention relates to the preparation of a polymer quantum dot, in particular to the preparation of a dual fluorescent polymer quantum dot material; the dual fluorescent polymer quantum dot material can gather in Hela cytoplasm and exhibit high fluorescence, so it can be used as Cell tracking agents, etc., have been better applied in the biological field. Background technique [0002] Polymer Dots (Polymer Dots) are organic fluorescent nanoparticles prepared from polymer materials with fluorescent effects. Different from inorganic semiconductor quantum dots, they have stronger fluorescence brightness, more stable optical properties, and can control and many other advantages. Polymer quantum dots generally have both hydrophilic and lipophilic ends, and can self-assemble in water-oil systems. Tetraphenylethylene is a typical aggregation-induced luminescence (AIE) lipophilic small molecule. In dilute solution, the tetraphenylethylene molecule...

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Problems solved by technology

However, in cell imaging, many organelles and proteins will produce green background fluorescence, which seriously affects the application and deve...

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