Carboxylation chitosan nano-fluorescence probe with aggregation-induced emission characteristic and preparation method thereof

A nano-fluorescent probe, aggregation-induced luminescence technology, applied in chemical instruments and methods, luminescent materials, fluorescence/phosphorescence, etc., can solve the problems of fluorescent probe light instability, achieve uniform size, wide pH soluble, sensitivity high effect

Inactive Publication Date: 2016-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, traditional fluorescent probes have defects such as photoinstability, photobleaching, and aggregation-induced quenching effects, whi

Method used

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  • Carboxylation chitosan nano-fluorescence probe with aggregation-induced emission characteristic and preparation method thereof
  • Carboxylation chitosan nano-fluorescence probe with aggregation-induced emission characteristic and preparation method thereof
  • Carboxylation chitosan nano-fluorescence probe with aggregation-induced emission characteristic and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Weigh 1g of carboxylated chitosan (viscosity-average molecular weight is 10,000, degree of acetylation is 20%, degree of carboxylation 20%) is added into an eggplant-shaped bottle, dispersed in 10mL DMSO, swelled at 50°C for 12h, and then added to Add TPEITC in the eggplant-shaped bottle, the molar ratio of TPEITC and the amino group in chitosan is 1%, react for 24h, obtain solution A and pour it into a 250mL beaker;

[0018] 2) Add 100mL tetrahydrofuran to solution A, stir evenly, let it stand until the solution is separated, remove the supernatant, centrifuge, dissolve the obtained precipitate with 20mL absolute ethanol and ultrasonically disperse for 20min, centrifuge, and dissolve the precipitate with 5mL triple distilled water Dissolve to obtain solution B;

[0019] 3) Put solution B into a dialysis bag with a molecular weight cut-off of 3500 and dialyze in three-distilled water for 4 days, freeze-dry to obtain fluorescently labeled carboxylated chitosan TPE-NCS...

Embodiment 2

[0023] 1) Weigh 1g of carboxylated chitosan (viscosity-average molecular weight is 50,000, degree of acetylation is 15%, degree of carboxylation 30%) is added into an eggplant-shaped bottle, dispersed in 10mL DMSO, swelled at 50°C for 12h, and then added to Add TPEITC to the eggplant-shaped bottle, the molar ratio of TPEITC and amino groups in chitosan is 5%, react for 24h, obtain solution A and pour it into a 250mL beaker;

[0024] 2) Add 100mL tetrahydrofuran to solution A, stir evenly, let it stand until the solution is separated, remove the supernatant, centrifuge, dissolve the obtained precipitate with 20mL absolute ethanol and ultrasonically disperse for 20min, centrifuge, and dissolve the precipitate with 5mL triple distilled water Dissolve to obtain solution B;

[0025] 3) Put solution B into a dialysis bag with a molecular weight cut-off of 3500 and dialyze in three-distilled water for 4 days, freeze-dry to obtain fluorescently labeled carboxylated chitosan TPE-NCS; ...

Embodiment 3

[0030] 1) Weigh 1g of carboxylated chitosan (viscosity-average molecular weight is 200,000, degree of acetylation is 10%, degree of carboxylation is 35%), add it into an eggplant-shaped bottle, disperse in 10mL DMSO, swell at 50°C for 24h, and then add to Add TPEITC to the eggplant-shaped bottle, the molar ratio of TPEITC and amino groups in chitosan is 10%, react for 36h, obtain solution A and pour it into a 250mL beaker;

[0031] 2) Add 100mL tetrahydrofuran to solution A, stir evenly, let it stand until the solution is separated, remove the supernatant, centrifuge, dissolve the obtained precipitate with 20mL absolute ethanol and ultrasonically disperse for 20min, centrifuge, and dissolve the precipitate with 5mL triple distilled water Dissolve to obtain solution B;

[0032] 3) Put solution B into a dialysis bag with a molecular weight cut-off of 3500 and dialyze in three-distilled water for 4 days, freeze-dry to obtain fluorescently labeled carboxylated chitosan TPE-NCS;

...

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Abstract

The invention discloses a carboxylation chitosan nano-fluorescence probe with aggregation-induced emission characteristic. A preparation method of the probe comprises the following main steps: labeling tetraphenylethylene fluorescence molecule (TPE) to a carboxylation chitosan chain (NCS) to obtain TPE-NCS with aggregation-induced emission (AIE) characteristic; and using sodium polyphosphate by an ion-crosslinking method to prepare TPE-NCS nanoparticles. The prepared nano-fluorescence probe has good dispersity in an aqueous solution, and has aggregation-induced emission characteristic. In comparison with a traditional fluorescence probe, the nano-fluorescence probe has advantages of high sensitivity, good light stability, no fluorescence spectra shift, stable and uniform nanoparticle size, easy storage and the like. Due to inherent characteristics of carboxylation chitosan, the probe of the invention has good biocompatibility, long retention of body circulation and good dispersity within a wide pH range, and could be used in fields of long-period cell tracking, pathological monitoring, drug metabolism detection, etc.

Description

technical field [0001] The invention relates to a carboxylated chitosan nanometer fluorescent probe with aggregation-induced luminescent properties and a preparation method thereof. Background technique [0002] Fluorescent probes use fluorescent substances as indicators, and under the excitation of a certain wavelength of light, the indicator will produce fluorescence, and the qualitative or quantitative analysis of the detected substance can be realized by detecting the generated fluorescence. At present, fluorescent probes are mainly used in the fields of biology, medicine, and environmental monitoring. A large number of corresponding probes have been explored for different needs. Generally speaking, they can be divided into three categories: traditional fluorescent probes, inorganic quantum Point probes, new fluorescent probes. Among them, traditional fluorescent probes have defects such as photoinstability, photobleaching, and aggregation-induced quenching effects, whi...

Claims

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Application Information

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IPC IPC(8): C08B37/08C09K11/06G01N21/64
CPCC08B37/003C09K11/06C09K2211/145G01N21/6486
Inventor 王征科刘亚蓝胡巧玲唐本忠
Owner ZHEJIANG UNIV
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