159 results about "Dendrimer" patented technology

The invention discloses a nano degradation agent for targeted degradation of PI3Kalpha protein as well as a preparation method and application of the nano degradation agent. The nano degradation agent comprises a polyamidoamine dendritic polymer G3-NH2, a PI3Kalpha protein targeted receptor and an HSC70 protein targeted receptor, wherein the PI3Kalpha protein targeted receptor and the HSC70 protein targeted receptor are coupled to the surface of the polyamidoamine dendritic polymer G3-NH2. The prepared nano degradation agent enters cells in an endocytosis mode, specifically targets corresponding treatment targets after entering the cells, and then efficiently degrades PI3Kalpha protein through a molecular chaperone-mediated autophagy pathway of lysosome, so that the PI3Kalpha protein can be effectively degraded. The nano degradation agent provided by the invention can be applied to intervention of related diseases caused by PI3Kalpha protein abnormality.

The invention relates to exosome-coated dendrimer nanogel co-loaded with gold nanoparticles and antibiotics as well as a preparation method and application of the exosome-coated dendrimer nanogel, the dendrimer nanogel is taken as a carrier, the gold nanoparticles and drugs are co-loaded in the carrier, and the surface of the carrier is coated with exosome. The operation condition is simple, purification is easy, the bioavailability of antibiotics can be improved, targeted delivery and responsive release of the medicine at the focus position are achieved, and the good application prospect in pulmonary tuberculosis treatment is achieved.

A preparation method for a micro-vehicle formed by enveloping siRNA-loaded polypeptide dendrimer nanogel with a cell membrane of engineered MSCs, and the use of same. The method comprises: using lentivirus-mediated gene transfection technology for constructing MSCs that highly express CXCR4 and PSGL-1; using cytokinin B for stimulating a cell to generate vesicles, and then performing centrifugation to collect the vesicles; by means of the electrostatic attraction between polypeptide dendrimer nanoparticles and siRNAs, attaching the siRNAs of IL-6 and HIF-1α into the polypeptide dendrimer nanoparticles; and by means of using a membrane extruder, enveloping the siRNA-loaded polypeptide dendrimer nanogel with the cell membrane. The prepared micro-vehicle exhibits a good property of aggregating at inflammatory blood vessel sites and a targeting function for joint cavities with rheumatoid arthritis. In addition, the loaded siRNAs can inhibit the expressions of IL-6 and HIF-1α in the joint cavities and inhibit the cell migration and angiogenesis. The micro-vehicle targeting inflammation sites has the advantages of excellent biocompatibility, ideal siRNA loading ratio, good inflammation targeting function, etc.

The invention relates to an exosome-coated phosphorus-containing dendrimer nano-composite as well as a preparation method and application thereof. The nano-composite is prepared from exosome derived from mesenchymal stem cells, a hydroxyl-terminal phosphorus-containing dendrimer and quercetin. The exosome derived from the mesenchymal stem cells is used as a carrier, so that not only are good biocompatibility and anti-inflammatory activity achieved, but also the bioavailability of the phosphorus-containing dendrimer and quercetin can be improved. The phosphorus-containing dendrimer and quercetin exert anti-inflammatory and anti-oxidation effects by promoting microglial cells to be polarized to M2 type and removing active oxygen, so that neuronal cells are protected from apoptosis. The preparation method has the advantages of being simple, easy to operate, good in biocompatibility and the like, and the provided strategy has good development prospects and application values in treatment of Parkinson's disease or other neurodegenerative diseases.

The invention belongs to the technical field of biological medicine, discloses a novel venom polypeptide composite dendrimer as well as a preparation method and application thereof, and particularly discloses scorpion venom polypeptide, and the amino acid sequence of the scorpion venom polypeptide is FLGGSLSSIF. According to the invention, a novel scorpion venom polypeptide (marked as C9) is separated and identified, and a novel polypeptide-polyamidoamine dendritic compound G5C9 (i.e., nanoparticles) with a glioblastoma (GBM) targeting effect is constructed. Compared with the polypeptide C9, the cell uptake efficiency of the G5C9 is obviously improved. G5C9 can enter cells through endocytosis and target lysosome to destroy functions of the lysosome and mTORC1, so that nuclear translocation of TFEB is promoted, and autophagic death of the cells is induced. Therefore, the G5C9 provides a novel mode for targeting the anti-GBM in vitro and in vivo.

The application relates to the field of supramolecular chemistry and discloses a dendrimer supramolecular gel factor and application thereof. The molecular structure of the gel factor is a polybenzyl ether dendrimer modified by peripheral halogen atoms (chlorine, bromine or iodine). The gelation performance of the gel factor can be precisely controlled by changing the type, substitution number and position of the peripheral halogen, and the type and generation of the dendrimer. The dendrimer gel factor provided by the application has very excellent gelation performance. The dendrimer gel factor can effectively gelate in dozens of solvents such as polar solvents, non-polar solvents and mixed solvents containing water, and the minimum gelation concentration CGC is 1.5 mg / mL, belonging to a super gel factor. In addition, the gel factor has a simple preparation method and high yield, and is very suitable for being developed into a general and commercial supramolecular gel factor and applied to the preparation of various soft material functional materials.

The invention relates to a tirapazamine-loaded phosphorus-containing dendrimer / protein compound for inducing tumor mineralization as well as a preparation method and application thereof, the compound takes diphosphonate-terminated phosphorus-containing dendrimer AK176 as a carrier, and the carrier is loaded with protein and medicine. The nano-drug can target tumor cells through FN and form a biomineralization layer in the peripheral area of tumor tissues, and a tumor hypoxic microenvironment is intensified, so that the pharmaceutical activity of tirapazamine is activated, and cooperative treatment of blocking treatment / chemotherapy of triple-negative breast cancer is realized.

The invention relates to a supramolecular nano gene vector as well as a preparation method and application thereof. The supramolecular nano-carrier is prepared from beta-cyclodextrin grafted polyethyleneimine, 8-adamantane grafted polyamide-amine dendrimer, adamantane grafted polyethylene glycol and adamantane grafted polyethylene glycol modified by using a cell penetrating peptide (TAT). A CRISPR / Cas9 system is loaded through self-assembly of a nano material monomer, the prescription is screened, and the subsequent research on cells and retinal organs proves that the transfection efficiency and the RS1 protein expression quantity are good. The supramolecular nanoparticle loaded gene editing system has a good potential of treating the X-linked retina cleavage disease. Moreover, the supramolecular nanoparticle gene vector is high in biological safety, high in transfection efficiency, simple and easily available in preparation mode, and good in clinical application potential in the future.

The invention relates to hydroxylated polyamide-amine dendrimer integrated drug-loaded nanogel as well as a preparation method and application thereof. The drug-loaded nanogel comprises third-generation polyamide-amine dendrimer integrated nanogel modified by double bonds and high-density hydroxyl groups, MnO2 nanoparticles and quercetin, wherein the nanogel is loaded with MnO2 nanoparticles in situ, and quercetin is physically wrapped in the nanogel. The drug-loaded nanogel disclosed by the invention can enhance a blood-brain barrier penetrating effect, regulate microglial cells and neurons in a double-target manner and remodel a brain inflammation microenvironment, so that neuroinflammation and dyskinesia of a Parkinson's disease model mouse are relieved, and the curative effect of the Parkinson's disease is improved. The compound has good development prospect and application value in treatment of Parkinson's disease or other neurodegenerative diseases.

The application provides a kind of smart parent tree macromolecule based on DASA and a preparation method thereof.Hydrophilic alkoxy ether dendritic unit, combined with hydrophobic visible light responsive DASA, constructs a new type of smart parent tree macromolecule based on DASA.The parent tree macromolecule of the application can self-assemble in aqueous solution and its self-assembly behavior can be controlled by light and temperature.The unique topological structure of alkoxy ether dendritic unit, crowding synergistic effect and temperature-induced multi-stage dehydration collapse mechanism can construct a hydrophobic microenvironment, which can be regulated by changing the end group structure of alkoxy ether unit, alkoxy chain length and alkoxy ether arm number, so as to realize the regulation of the reversible isomerization behavior of DASA in aqueous solution.In addition, the raw materials are environmentally friendly, cheap, low toxicity and have good biocompatibility, which shows great application potential in the fields of biomedicine and photosensitive materials.

The invention relates to the technical field of oil exploitation, and relates to an alcohol-based fracturing fluid containing modified nanoparticles and a preparation method thereof, the alcohol-based fracturing fluid comprises the following components: an alcohol solvent, modified nanoparticles, an anti-swelling agent, a thickening agent, a polyamide-amine dendritic polymer, a pH regulator, an optional suspending agent and water; the modified nano-particles are prepared by modifying nano-particles with an alkenyl silane coupling agent, then copolymerizing the modified nano-particles with alkenyl-containing imidazolium salt, and grafting polyimidazolium salt on the surfaces of the nano-particles in situ. The preparation method comprises the following steps: modifying nanoparticles by using an alkenyl silane coupling agent, copolymerizing with alkenyl-containing imidazolium salt to form polyimidazolium salt, and grafting the polyimidazolium salt on the surfaces of the nanoparticles in situ to form modified nanoparticles; the anti-swelling property, the cross-linking property and the sand-carrying capacity of the alcohol-based fracturing fluid are remarkably improved through the combined action of the modified nanoparticles and the polyamide-amine dendritic polymer, and the gel forming and hanging time is 60-90 s, so that the alcohol-based fracturing fluid is particularly suitable for medium-high-temperature deep wells.

The invention relates to a cationic carbosilane dendrimer comprising a cyclotetrasiloxane core, wherein each silicon of the core is bonded by means of a carbosilane structure (CH2)2{Si(Me)2}(CH2)2S(CH2)2 to a peripheral structure comprising an ammonium group bonded by means of an alkyl chain (CH2)2 to a hydroxyl group. The invention also relates to the use of the dendrimer to remove at least one pathogenic species, either in suspension or adhered to a surface, forming a biofilm, and to a transport vehicle of at least one drug. The invention further relates to the use of the dendrimer as a drug or in the prevention and / or treatment of at least one disease caused by at least one pathogen.

The invention belongs to the technical field of medical polymer materials, and discloses hydrogel as well as a preparation method and application thereof. The hydrogel comprises the following raw materials: a macromonomer, a polyamidoamine dendrimer, a photoinitiator, a delayed photosensitizer and a solvent, the mass ratio of the macromonomer to the polyamidoamine dendrimer is (1: 10)-(10: 1). The hydrogel has high transparency and excellent puncture resistance, and a needle passage left after the hydrogel is punctured can be quickly self-repaired, so that no trace is left after repeated puncture, and ultrasonic imaging is prevented from being interfered.

The application relates to the field of supramolecular chemistry, and discloses a peripheral trifluoromethyl functionalized polybenzyl ether dendrimer gelator, a preparation method and application thereof. The peripheral trifluoromethyl functionalized polybenzyl ether dendrimer gelator is shown in the following formula, can form stable supramolecular organic gels or supramolecular self-assembly systems in various organic solvents or mixed solvents through the synergistic effect of multiple pi-pi interactions between peripheral aromatic rings and F...H weak hydrogen bonds, has a minimum gelation concentration CGC of 3.2 mg / mL, and is easy to be functionally modified, for example, a gel with an aggregation-induced emission performance is prepared by introducing 1-pyrene or 9-anthracene functional groups. The preparation method of the gelator is efficient, high-yield and high-purity.

The present disclosure provides for a method of producing an amine from an alcohol that includes supplying a heterogeneous supported metal catalyst, where the heterogeneous supported metal catalyst comprises nanoparticles of an alloy encapsulated with a dendrimer on a solid support, where the alloy is of Formula I: where Pd is palladium; x is an integer of 20 to 30; y is an integer of 10 to 20, and M is selected from the group consisting of nickel (Ni), cobalt (Co), ruthenium (Ru) and rhodium (Rh), with the caveat that when y is 10, M is not Ni; mixing the heterogeneous supported metal catalyst with a first reagent containing an alcohol moiety and a second reagent containing an amine moiety to form a mixture; and heating the mixture to a predetermined temperature for a predetermined time to produce an amine compound.

The invention provides a dendrimer-modified phthalocyanine photosensitive compound and application thereof, the dendrimer-modified phthalocyanine photosensitive compound has any one structure as shown in formulas I-IV, and the photosensitive compound has the advantages of simple and controllable synthesis, clear structure, strong light killing ability and the like. The water solubility, tumor targeting and biocompatibility of the zinc phthalocyanine photosensitizer can be improved, the defects of a traditional nano photosensitizer delivery system can be overcome, and the zinc phthalocyanine photosensitizer has a good application prospect in the aspect of photodynamic therapy of tumors.

The invention relates to pH-ROS (potential of hydrogen) double-response type drug-loading dendrimer nanogel as well as a preparation method and application thereof. According to the pH-ROS double-response type drug-loading dendrimer nanogel, G3-PBA-OH is obtained after a G3 PAMAM (polyamidoamine) dendrimer is subjected to functional modification; cross-linking with polyethylene glycol PBA-PEG-PBA with two ends grafted with phenylboronic acid to obtain dendrimer nanogel DNG, and further loading hypoxia activated chemotherapeutic drugs and cytokines on the DNG to obtain the hypoxia activated chemotherapeutic drug-loaded dendrimer nanogel. The DNG prepared by the invention has the characteristics of good biocompatibility, high drug loading rate, pH-ROS dual response and blood brain barrier penetration, provides a new thought for constructing a brain drug delivery nano platform, and can significantly improve the bioavailability of TPZ and IFN-gamma.

A dendrimer that includes: (a) a central core; and (b)(i) at least one 2-deoxyglucose-surfaced branching units conjugated to the central core; or (b)(ii) at least one trehalose-surfaced branching units conjugated to the central core; or (b)(iii) at least one 2-deoxyglucose-surfaced branching units conjugated to the central core and at least one trehalose-surfaced branching units conjugated to the central core.

The present invention discloses an electrochemical sensor based on dendrimer functionalized Ni-MOFs nanocomposite material, a preparation method and an application thereof. First, the dendrimer functionalized Ni-MOFs nanocomposite material is prepared by using polyamidoamine dendrimer PAMAM, HITP ligand and NiCl2. Then, the obtained dendrimer functionalized Ni-MOFs nanocomposite material is used to modify a glassy carbon electrode to obtain a functionalized electrode. The functionalized electrode is used as a working electrode, a platinum electrode is used as a counter electrode, and an Ag / AgCl electrode is used as a reference electrode to form a three-electrode system, i.e., an electrochemical sensor based on the dendrimer functionalized Ni-MOFs nanocomposite material. The present invention is a Pb 2+ 、Cu 2+ The simultaneous quantitative detection of β-catenin provides an analytical method that is simple, effective, low-cost and highly sensitive.

The present invention relates to the field of polymer compounds, relating to a water-dispersible copolymer, a preparation method therefor, and a use thereof. The water-dispersible copolymer provided by the present invention comprises repeat units generated from a monomer I, repeat units generated from a monomer II, and repeat units generated from a monomer III. Monomer I is a carbosiloxane dendrimer monomer, monomer II is a cationic monomer having a tertiary amine group, and monomer III is a hydrophilic monomer. The copolymer, or a composition containing said polymer, can be used for surface treatment of paper, and imparts strong water and oil repellent properties to the paper surface.

The invention discloses a preparation method and application of a tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug, and belongs to the technical field of biological medicines. According to the present invention, M2 type TAM targeting peptide CRVLRSGSC modified dendrimer PAMAM is adopted to jointly load small activation RNA (sa-p38, sa-TFEB) of p38 and TFEB, and acid-sensitive liposome is adopted to wrap and protect the compound (CRV-PAMAM / saRNA) to prepare the tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug, and the phenotype and the metabolic mode of M2 type TAM can be reprogrammed into M1 type TAM so as to kill tumor cells, such that the tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug can be used for preparing the tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug, and the tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug can be used for preparing the tumor microenvironment response type cascade targeting small activation nucleic acid nano-drug. And a tumor immune microenvironment is remodeled, and finally, tumor growth is inhibited and even tumors are ablated. The invention has a very wide application prospect in gene therapy and immunotherapy of cancers, hereditary diseases, infectious diseases and the like.

Dendrimer formulations comprising one or more fantastic or fantastic agents and methods of using the same are described. Preferably, the dendrimer conjugates selectively bind to one or more receptors on the surface or within the target cell. The formulations are suitable for enteral and / or parenteral administration for the treatment of one or more receptor-mediated diseases, including psychological, cognitive, behavioral and / or mood disorders.

The invention relates to amphiphilic Janus dendrimers which may form nanoparticles. The invention also relates to methods of delivering an agent to a target of interest (e.g., lung, liver, lymph nodes, and spleen) using said nanoparticle. In some aspects, the present invention also relates to methods of inducing an adaptive immune response in a subject comprising administering to the subject an effective amount of a composition comprising at least one nucleoside-modified RNA encoding at least one antigen and at least one amphiphilic Janus dendrimer.

The invention discloses a borneol modified erythrocyte membrane coated dendritic macromolecule bionic nano drug delivery system and preparation and application thereof, the bionic nano drug delivery system comprises an erythrocyte membrane and a drug loaded dendritic macromolecule coated in the erythrocyte membrane, the surface of the erythrocyte membrane is modified with targeted molecule borneol, the drug is an anti-tumor drug, such as bufotalin. In combination with a nasal drop administration mode, not only can the brain targeting efficiency of the glioma treatment drug be improved, but also the glioma treatment drug has immune escape and tumor microenvironment response capabilities, the in-vivo circulation time of the drug is effectively prolonged, and the glioma treatment effect is further improved. The design that the dendritic macromolecules are coated with the modified erythrocyte membranes combines the biocompatibility of natural cell membranes and the multifunctionality of nanoparticles, and the dendritic macromolecules have huge application potential in the fields of drug delivery, immune regulation, detoxification, targeted therapy and the like.

The invention relates to ionizable amphiphilic Janus dendrimers with different linker lengths. In some embodiments, the different linkers enable delivery of the agent to a specific target of interest (e.g., kidney, heart, lung, liver, lymph nodes, spleen, or any combination thereof). In some embodiments, the invention relates to nanoparticles comprising said ionizable amphiphilic Janus dendrimers. In some embodiments, the invention relates to compositions comprising said ionizable amphiphilic Janus dendrimers. In some aspects, the present invention also relates to methods of inducing an adaptive immune response in a subject comprising administering to the subject an effective amount of said nanoparticle or said composition.

The present invention discloses a collagen matrix flap covalently bound to fibroblast growth factor and a preparation method thereof. The collagen matrix flap is prepared by cross-linking oxidized sodium alginate (OSA) and bovine type I collagen. Subsequently, polyamide-amine dendrimer (PAMAM) is used as a connection carrier to covalently graft basic fibroblast growth factor (bFGF), thereby constructing a scaffold system capable of long-term sustained release of growth factors. The scaffold has good biocompatibility. Experiments have shown that the scaffold of the present invention exhibits clinical operability, superior biological activity to other growth factor-loaded scaffolds, and a more sustained growth factor release effect.

The invention particularly relates to an endogenous and endogenous dual nano sensitizer, a preparation method of the endogenous and endogenous dual nano sensitizer and application of the endogenous and endogenous dual nano sensitizer in preparation of brain glioma drugs. The endogenous and exogenous dual nano sensitizer comprises a glycosylated dendrimer Gal-G5 modified compound norcantharidin (DMC) and nano particles internally wrapped with gold (Au). The preparation method and the application comprise the following steps: preparation of Gal-G5, preparation of Gal-G5-DMC, and preparation of the internal and external source dual nano sensitizer Gal-G5 (Au)-DMC. The preparation method is simple, the required raw materials are easy to obtain, the synthesis process is simple and convenient, separation and purification are easy, and the synthesized endogenous and endogenous dual nano sensitizer has the capability of crossing a blood brain barrier to reach brain glioma, can be used for dual sensitization of brain glioma radiotherapy, can also be combined with an immune checkpoint inhibitor (anti-PD-L1 antibody) for combined application of radiation-immunotherapy, and has broad application prospects. And potential application prospects are realized in the aspects of radiotherapy and immunotherapy.

To provide a hematoxylin formulation comprising racemic hematoxylin.SOLUTION: A first aspect of the present disclosure is a hematoxylin formulation including racemic hematoxylin and at least one additive (e.g., at least one additive selected from a solvent, a chemical oxidant, a mordant, a stabilizer, and an antioxidant). Another aspect is a method for staining a biological sample, comprising contacting the biological sample with a racemic hematoxylin formulation. In some embodiments, the racemic hematoxylin formulation includes a solvent, hematoxylin, an amount of a chemical oxidant sufficient to convert at least a portion of the hematoxylin into hematein, a mordant, an antioxidant, and a stabilizer. In some embodiments, the stabilizer is selected from the group consisting of a polysaccharide, a cryptand, a cryptophane, a cavitand, a crown ether, a dendrimer, a nanotube, a calixarene, a valinomycin, and a nigericin.SELECTED DRAWING: None

The invention provides an application of a dendrimer modified perovskite nanocrystal probe in prediction of mammary gland lymph node metastasis. The probe is formed by coupling a biological material of a targeted EPN3 gene and a water-soluble perovskite nanocrystal through electrostatic interaction, the water-soluble perovskite nanocrystal is formed by packaging perovskite quantum dots and a dendritic macromolecular packaging material through the coordination bonding effect of chemical bonds. The EPN3 modified water-soluble perovskite nanocrystalline probe provided by the invention can be used for high-sensitivity rapid detection of tumor tissues or cells with breast cancer lymph node metastasis.