The invention discloses a method for preparing micro-particle-size W/O/W multiple emulsion carrying a medicine, which belongs to the technical field of medicinal preparations. The method comprises three steps: 1, dissolving the medicine to be carried and electrolyte for providing a osmotic pressure condition in a water phase together, emulsifying the water phase and an oil phase containing lipophilic emulsion stabilizer, and obtaining W/O initial emulsion; 2, mixing the initial emulsion and an external water phase containing a hydrophilic emulsion stabilizer, roughly dispersing and obtaining coarse multiple emulsion with a big particle size; and 3, diluting the coarse emulsion with deionized water, stirring gently, and obtaining the multiple emulsion product with a micro particle size. The principle of the preparation method is to promote emulsion drops of the coarse multiple emulsion to expand and break under a diluted condition by regulating the osmotic pressure of the internal water phase of the multiple emulsion so as to produce the multiple emulsion drops with a particle size of 1 to 10 micro meters. In the invention, the operation conditions are mild, the particle sizes of the multiple emulsion drops are small, the particle size range is narrow, the medicine entrapment rate is high, the stability of the preparation is high, the controlled-release and sustained-release effects on the carried medicine are good, and the lymph affine effect and targeted release effect of the emulsion are played more fully.