663 results about "Immune microenvironment" patented technology

The invention provides a tumor detection data analysis method based on a peripheral blood immune marker, and belongs to the technical field of immunodetection.The tumor detection data analysis method comprises the steps that firstly, a peripheral blood sample of a patient is collected, and plasma and cell components are separated; analyzing various immune cell phenotypes by using flow cytometry; determining the concentration of key cell factors; analyzing the molecular expression level of the immune checkpoint; establishing an immune cell subset proportion empirical function; analyzing a cell factor expression mode by using an immune lineage transcriptome analysis model; establishing a tumor immune microenvironment score by combining the immune cell subset proportion and a model output result; the accuracy of the scoring system is verified through clinical follow-up visit and iconography examination; a standardized report template is formulated to provide a tumor early detection basis. Wherein the immune lineage transcriptome analytical model adopts a multi-modal attention fusion network architecture, three different immune data streams can be processed and integrated at the same time, and accurate evaluation of tumor-related immune states is realized.

The invention provides a tumor cell exosome, an anti-tumor immune drug delivery system and a preparation method and application of the anti-tumor immune drug delivery system, the tumor cell exosome is derived from starved and stimulated tumor cells, the tumor cell exosome is coated with nano drug particles for delivery, and the exosome has the effects of enhancing immune cell infiltration and remodeling an immune microenvironment; due to the homing effect of the exosome, the medicine and the exosome are more easily accumulated in the tumor, and the targeting property is improved; due to coating and camouflage of the biological membrane of the exosome membrane, the exosome membrane is prevented from being recognized, attacked and removed by an organism, and the biological safety is improved, so that the exosome and the nano-drug act on the tumor together to achieve a synergistic effect.

The invention discloses a lung adenocarcinoma prognosis risk prediction model and a construction method and application thereof, and belongs to the technical field of biomedical detection. The lung adenocarcinoma (LUAD) prognosis model is successfully constructed by systematically analyzing the immune infiltration related gene (CAFRG) of tumor-associated fibroblasts (CAF), and the application value of the lung adenocarcinoma (LUAD) prognosis model in prediction of patient prognosis is verified. The CAFRG risk score is negatively related to the immune microenvironment score and is remarkably related to immune checkpoint gene expression, which suggests that a high-risk patient may have the characteristic of immune escape. Furthermore, based on TIDE tool analysis, the patients in the low-risk group have more active T cell immune response. The risk score is also closely related to the sensitivity of antitumor drugs, especially Doramapimod. Therefore, a new theoretical basis is provided for personalized treatment of lung adenocarcinoma, and a new direction is provided for future clinical research.

The invention discloses a medicine for targeting senescent cells to stimulate endogenous immunity and application of the medicine, and relates to the field of biological medicine. The medicine provided by the invention can mediate the interaction between senescence cells and immune cells, enhance the endogenous immune function, and achieve the purposes of effectively removing senescence cells and improving senescence-related diseases. According to the functional molecule for targeted aging cell immune sensitization, one end of the molecule targets an aging cell membrane molecule, glutamic acid is used for marking the surface of an aging cell, coupling between an endogenous immune cell and the aging cell is mediated, and adhesion and removal of the aging cell by the immune cell are promoted. The molecule can regulate the immune microenvironment of senescence tissues, effectively reduce the number of positive senescence cells of senescence-related beta-galactosidase, reverse fibrosis and improve natural senescence phenotypes.

The invention relates to the technical field of tumor prognosis prediction, in particular to a chest tumor prognosis prediction method and system based on data analysis, and the method comprises the following steps: S1, data collection: collecting multi-modal data of a chest tumor patient; s2, data preprocessing: preprocessing the collected multi-modal data; s3, prediction of tumor immune cell infiltration: predicting the infiltration degree of tumor immune cells, and evaluating the influence of the infiltration degree on patient prognosis; and S4, prognosis analysis and prediction: performing comprehensive analysis through a multi-layer perceptron algorithm to generate prognosis prediction results of the tumor patient, including tumor metastasis risk, recurrence probability and immunotherapy response. According to the invention, the complexity of the tumor immune microenvironment can be better captured, so that more accurate prognosis evaluation is provided, and clinical doctors are helped to understand the potential influence of immune response on tumor metastasis, recurrence and immunotherapy response.

The invention discloses a three-section self-assembly composite polypeptide nanoparticle for targeted repair of endothelial barrier and regulation and control of immune recruitment cascade and a preparation method and application thereof, and the preparation method comprises the following steps: (1) preparation of a hydrophilic section head multifunctional peptide solution, (2) preparation of a middle section connector ROS response thioketal solution, and (3) preparation of a three-section self-assembly composite polypeptide nanoparticle for targeted repair of endothelial barrier and regulation and control of immune recruitment cascade. (5) preparing a three-section type compound of a hydrophilic section, the hydrophobic section and the middle section, and (6) mixing the connection compound solutions of the three types of polypeptide of the hydrophobic section, the middle section and the hydrophilic section, and dialyzing to remove DMSO (Dimethylsulfoxide), so that the compound solution is changed from an organic phase to a water phase, and the three-section type compound of the hydrophobic section, the middle section and the hydrophilic section is obtained. And promoting amphiphilic self-assembly of the compound and co-assembly of the three polypeptide compounds, and freeze-drying to obtain the three-section type self-assembly composite polypeptide nanoparticles. According to the invention, inflammatory endothelial cells are targeted, local high ROS microenvironment is responded, endothelial barrier repair peptide is released to repair endothelial barrier, complement receptor antagonistic peptide is released to regulate complement cascade reaction so as to recover immune microenvironment, and body inflammation and immune disorder are cooperatively regulated.

The invention discloses an experimental method for relation between cytokine level and tumor immune microenvironment in circulation, which comprises the following steps of: S1, collecting colorectal cancer patient data and normal data for contrast, and preferably screening a gene expression data set from a Gene Expression Omnibus (GEO) database; according to the method, a plurality of gene expression data sets from the GEO database are collected and analyzed, so that differential expression genes between colorectal cancer and normal tissues can be systematically identified, and a solid foundation is provided for subsequent research. The process not only covers batch correction, normalization and batch effect elimination of data, but also visually presents data distribution changes through principal component analysis, ensures the accuracy and reliability of analysis results, confirms that IL6 and IFN gamma are one of the most critical factors affecting the colorectal cancer immune microenvironment, and participate in formation of immune infiltration.

The method and system for evaluating the curative effect of hepatocellular carcinoma based on immune microenvironment characteristics are high in accuracy and reliability of evaluating the curative effect of hepatocellular carcinoma. The method comprises the following steps: (1) preparing a sample; (2) carrying out multiple immunohistochemical staining; (3) image acquisition and analysis; (4) analyzing spatial distribution characteristics; (5) multiplying the input image voxel signal numerical value by the weight vector of the convolution kernel, transmitting the multilayer convolution kernel, and finally obtaining a calculation result output by each network; and (6) establishing a prediction model: based on the spatial distribution characteristics and clinical characteristics of immune cells, constructing the prediction model by using the positive rate of CD8 + PD-L1 + and CD103 + CD8 + cells in the total area and the number of CD103 + cells within 30 microns around tumor cells so as to predict the immunotherapy curative effect of the HCC patient.

The invention provides lipidosome wrapping L-arginine / Prussian blue nano particles and a preparation method and application thereof, and belongs to the technical field of biological agents. L-arginine is deposited on hPB through iron chelation, then the lipidosome is coated to avoid leakage of water-soluble L-arginine in circulation, and thus the L-arginine / Prussian blue nano particles are obtained. The liposome coated with the L-arginine / Prussian blue nano particles is obtained. Due to the EPR effect, the hPLL-coated Lipo can be efficiently transferred and distributed into tumor tissues, heat is generated under the near-infrared light irradiation condition to ablate a large number of tumor cells, the utilization degree of the tumor cells to L-arginine is greatly limited, meanwhile, immunogenic death is induced, DAMPS is released in situ, infiltration and activation of anti-tumor T cells are further promoted, and the anti-tumor effect is achieved. The tumor immunotherapy is enhanced. And under illumination, the liposome disintegrates and releases the L-arginine, so that nutritional support is provided for immunological competence cells in competitive disadvantage, and activation of an immune microenvironment is promoted.

The invention discloses an establishment method and application of a mouse gastric cancer orthotopic transplantation model, and the establishment method comprises the following steps: injecting a mouse gastric adenocarcinoma cell line MKC1 (Mus musculus) into a mouse gastric tissue, and feeding to obtain the mouse gastric cancer orthotopic transplantation model. Compared with the prior art, the mouse gastric cancer in-situ model established in the invention has the advantages of strong repeatability in the construction process and good passage stability, especially can form tumor subcutaneously in a C57BL / 6 mouse with a sound immune system, has a therapeutic response to immunotherapeutic drugs such as anti-PD-1 monoclonal antibody, and is suitable for research on gastric cancer immune microenvironment and drug resistance mechanism of immunotherapeutic drugs.

The invention discloses a tetravalent platinum autophagy inducer with a structure of a general formula I as well as a preparation method and application of the tetravalent platinum autophagy inducer. The compound disclosed by the invention has an induction effect on cell autophagy, and can be used for degrading immunosuppressive protein tenascin-C (Tenascinin-C) through autophagy so as to improve a tumor immunosuppressive microenvironment. The tetravalent platinum complex induces tumor cells to generate autophagic death through unique structural characteristics, promotes immune killer cells to infiltrate a tumor immune microenvironment, can be used for preparing novel chemical immunotherapy drugs with multiple anti-tumor mechanisms, and has broad application prospects. The problems that the traditional treatment effect is poor and a small molecule immunotherapeutic agent is lacked are solved. # imgabs0 #

The invention belongs to the technical field of biological detection, and particularly relates to a prediction model for predicting prognosis after neoadjuvant immunotherapy of non-small cell lung cancer (NSCLC) through primary tumor (PT) and a construction method of the prediction model. According to the invention, a plurality of spatial high-throughput sequencing bioinformatics technologies are utilized to analyze a key immune cell subset, an immune microenvironment topological feature structure and a three-level lymphosome-related functional cell subset of a primary focus after neoadjuvant immunotherapy; the prediction model for predicting prognosis after neoadjuvant immunotherapy aiming at the NSCLC through the primary focus and the construction method of the prediction model are more in line with post-treatment immune microenvironment spatial histological characteristic changes, and compared with a previous transcriptome sequencing method without spatial information, the prediction model has the advantages that the prediction model is more efficient and more efficient; the change of a space tumor immune microenvironment after immunotherapy is more accurately represented, and the prognosis after NSCLC neoadjuvant immunotherapy can be more accurately predicted through cell targets really influencing the immune curative effect.

The invention relates to application of a GATA3 inhibitor and a PD1 inhibitor in preparation of a medicine for treating breast cancer, and belongs to the technical field of biological medicine. The invention discloses application of a GATA3 inhibitor and a PD1 inhibitor in preparation of a medicine for treating breast cancer. The GATA3 regulates the transcription regulation of IFNbeta by inhibiting the enhancer activity of IFNB1, down-regulates an I-type interferon signal channel, inhibits the secretion of an MHC-I antigen presentation channel and a chemotactic factor CCL5 of breast cancer cells, reduces immune recognition, and achieves the purpose of immune escape. The immunotherapy for inhibiting GATA3 combined with the PD1 inhibitor to treat breast cancer effectively treats breast cancer mice, improves the IFN beta level in the mice, promotes the MHC-I antigen presentation pathway of the mice, improves the CCL5 level in the mice, and improves the CD8 + T cell level in a tumor immune microenvironment, thereby reducing tumor occurrence and inhibiting tumor growth.

The invention relates to the technical field of cancer treatment, and provides application of a lactyltransferase inhibitor in pancreatic cancer and immunotherapy thereof. According to the present invention, the histone lactosyltransferase inhibitor and the anti-programmed death receptor-1 antibody are combined to form the composition for pancreatic cancer and immunotherapy thereof, such that the recognition and the removal of the immune system on the cancer cells can be enhanced, the growth of the pancreatic cancer tumor can be effectively inhibited, and the survival time of the pancreatic cancer patient can be prolonged. The histone lactosyltransferase inhibitor reduces the infiltration level of tumor-associated neutrophil, increases the infiltration level of CD8 positive T cells, reverses the composition of a pancreatic cancer inhibitory immune microenvironment, and generates a synergistic anti-tumor effect with an anti-programmed death receptor-1 antibody. The traditional Chinese medicine composition is suitable for early-stage, middle-stage and late-stage treatment of pancreatic cancer, improves the survival rate and the treatment effect of pancreatic cancer patients, remarkably reduces the side effects of treatment and improves the living quality of the pancreatic cancer patients.

The invention belongs to the technical field of biotechnology and animal models, and discloses a double-person-derived mouse model for simulating a tumor immune microenvironment and a construction method and application thereof. The method comprises the following steps: firstly, pretreating NSG immunodeficient mice by adopting low-dose whole-body irradiation in combination with double-antibody targeted bone marrow depletion, and transplanting CD34 + hematopoietic stem cells from the same human donor to complete human immune system reconstruction; separating tumor primary cells, tumor-related fibroblasts and tumor vascular endothelial cells of the same donor, performing three-dimensional co-culture to obtain homologous human tumor organs, and performing in-situ inoculation to immune reconstruction mice to obtain a target model. The core defects of MHC mismatching, low immune reconstruction efficiency, poor tumor immune microenvironment simulation degree, low clinical consistency and the like of an existing model are overcome, and the method can be used for tumor immune treatment drug screening, microenvironment mechanism research and personalized tumor treatment scheme verification.

The invention discloses a recurrence-resistant cell strain for colorectal cancer large segmentation radiotherapy and application, and relates to the technical field of cell biology. The colorectal cancer large segmentation radiotherapy recurrence-resistant cell strain is preserved in the China Center for Type Culture Collection on January 2, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C202533. By constructing the colorectal cancer large segmentation radiotherapy recurrence-resistant cell strain, a new biological material is provided for studying the radiotherapy recurrence-resistant colorectal cancer. The cell strain is screened in a mouse body with a sound immune system, has better physiological correlation, and is beneficial to research on the influence of an immune microenvironment on tumor cell radiotherapy resistance and the remodeling effect of radiotherapy resistant cells on the immune microenvironment. The cell strain can also monitor tumor recurrence after radiotherapy in real time through fluorescence imaging.

The invention discloses a mouse-derived gastric adenocarcinoma cell line MKC1 and application thereof. The mouse gastric adenocarcinoma cell line MKC1 (Mus musculus) is preserved in the China Center for Type Culture Collection, the preservation date is June 15, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024183. The cell line belongs to a murine gastric adenocarcinoma cell line, can be stably passaged, is high in growth speed, and can form primary tumors in a C57BL / 6 mouse body with a sound immune system. In addition, the cell line has the advantages of being high in repeatability, stable and single in property and the like, and is suitable for related research of gastric cancer immune microenvironment, tumor drug resistance, invasion and metastasis and the like.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention discloses application of docosapentaenoic acid or medicinal salt thereof in preparation of a medicine for preventing and / or treating systemic lupus erythematosus. The invention aims to provide novel application of docosapentaenoic acid in treatment of systemic lupus erythematosus. In view of various immune abnormalities existing in the systemic lupus erythematosus morbidity process, the docosapentaenoic acid has potential application value in the field of systemic lupus erythematosus treatment due to the effects of regulating immune cell functions and improving immune microenvironment.

A hybrid liver cancer prognosis model, prognosis system and medium, the model takes independent influence factors as input and outputs hybrid liver cancer postoperative risk scores, the hybrid liver cancer postoperative risk scores are used for dividing risk levels, and the risk levels are used for hybrid liver cancer prognosis evaluation. And / or is used for postoperative adjuvant chemotherapy decision-making of mixed liver cancer. The hybrid liver cancer prognosis model is jointly constructed by combining the traditional clinical pathological characteristics and immune microenvironment indexes, the key effect of the tumor immune microenvironment in CHC prognosis is indicated, and the constructed hybrid liver cancer prognosis model has good CHC prognosis prediction performance; in addition, decisions of postoperative adjuvant chemotherapy can be provided for patients with different risk levels, precise treatment of the CHC patients is achieved, and long-term survival prognosis of the CHC patients is improved.

The invention belongs to the technical field of disease drugs, and particularly relates to application of a recombinant attenuated coxsackie B3 virus loaded with an anti-inflammatory factor in preparation of drugs for treating traumatic craniocerebral injury. The virus can regulate and control a peripheral immune phenotype capable of inhibiting inflammation, after severe craniocerebral injury of a mouse, a host immune system is remodeled through single intraperitoneal injection treatment, a treatment effect is achieved in improving an immune microenvironment in the acute stage of TBI, the neuroprotective effect is observed in the early stage and the late stage of TBI, and safety is achieved.

The invention relates to the technical field of cell biology, in particular to a construction method and application of intestinal organs for children with Crohn's disease, the intestinal organs for children with Crohn's disease are successfully constructed by utilizing intestinal tissues from children with Crohn's disease, and the intestinal organs have various characteristic cells of the intestinal tissues; besides, immune cells are extracted from peripheral blood of a child patient homologous with intestinal tissues, a co-culture system is established with the organ-like model, the intestinal immune microenvironment of the child patient suffering from the Crohn's disease can be reproduced, and the system provides a new model for related mechanism exploration and drug screening of the child Crohn's disease.

The invention relates to a pathological pulmonary fibrosis model and a construction method and application thereof. The pathological pulmonary fibrosis model provided by the invention is good in stability and controllability and high in maturity, not only can better simulate the function and structural characteristics of pulmonary alveoli-capillary barrier, but also has the pathological characteristics and immune microenvironment of pulmonary fibrosis lesions, has the advantage of high simulation degree, and has good application prospects in simulation and research of pulmonary fibrosis diseases. Good application prospects are realized in the aspects of drug detection, screening, evaluation, research and development and the like.

The invention provides a preparation method and application of a modified chitosan derivative and nanoparticles thereof. The modified chitosan derivative is prepared by modifying chitosan with nitrogen-containing heterocyclic ring small molecules; the modified chitosan derivative nano-particles are prepared by electrostatic compounding of a modified chitosan derivative on an electronegative substance. The modified chitosan derivative and the nano-particles thereof have relatively strong immune activation capability on immune cells, and also can enhance the immunogenicity of tumor cells and effectively regulate the immune microenvironment of solid tumors. When the modified chitosan derivative and the nano-particles thereof are used in combination with radiotherapy, the modified chitosan derivative and the nano-particles thereof have relatively high tumor treatment efficiency.

The invention discloses a CBLN1 monoclonal antibody and an application thereof. The CBLN1 monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the light chain variable region comprises three complementary determining regions CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is shown as SEQ ID NO.1, the amino acid sequence of the CDR2 is KVS, and the amino acid sequence of the CDR3 is shown as SEQ ID NO.2; the amino acid sequences of three complementary determining regions CDR1, CDR2 and CDR3 of the heavy chain variable region are respectively as shown in SEQ ID NO. 3, SEQ ID NO. 4 and SEQ ID NO. 5. The invention also discloses a preparation method of the monoclonal antibody and application of the monoclonal antibody in preparation of drugs for treating breast cancer and prostate cancer, especially castration-resistant prostate cancer and androgen receptor dependent prostate cancer. The CBLN1 specific monoclonal antibody can be used for functionally blocking the CBLN1 and improving the inhibition of a tumor immune microenvironment.

The invention discloses a method for promoting endothelialization of a heart valve and an artificial heart valve.The method is characterized in that circular DNA is modified on the surface of a decellularized valve (DHV), DNA hydrogel of targeted thrombin is obtained on the surface of the DHV through rolling circle amplification, the DNA hydrogel plays a role of a heme catcher while playing a role of the heme catcher, and heme is recruited from blood; heme is further converted into biliverdin (BV) through heme oxygenase (HO1) loaded in DNA, so that continuous production of biliverdin is ensured, anti-inflammatory immune regulation and active oxygen removal are promoted, a regenerative immune microenvironment is created, and endothelialization of the valve is promoted.

The invention relates to the technical field of biomedical materials, in particular to engineered small extracellular vesicle hydrogel enriched with PD-L1 and Siglec-15 as well as a preparation method and application of the engineered small extracellular vesicle hydrogel. The engineering small extracellular vesicle hydrogel is enriched with PD-L1 and Siglec-15 at the same time, the engineering small extracellular vesicle hydrogel comprises a hydrogel base body and PDL1-Siglec15-sEVs loaded in the hydrogel base body, and the PDL1-Siglec15-sEVs are small extracellular vesicles with the PD-L1 and the Siglec-15 in an overexpression mode. Compared with natural small extracellular vesicles and a traditional wound surface treatment strategy, the engineered small extracellular vesicle hydrogel has the advantages that PD-L1 and Siglec-15 can be enriched in the vesicles, so that more accurate immune microenvironment regulation and control can be realized on the local part of a wound surface, excessive inflammatory response is moderately inhibited, phenotype transformation of macrophages to be beneficial to tissue regeneration is promoted, and the wound surface treatment effect is improved. The hydrogel is used as a carrier and can provide a moist healing environment and a three-dimensional scaffold structure, so that the residence time of the engineered small extracellular vesicles on the local wound surface is remarkably prolonged, and slow release is realized.

The invention belongs to the technical field of high polymer material synthesis, and particularly relates to an artificial antioxidant enzyme material with an interlayer structure and a preparation method of the artificial antioxidant enzyme material. The preparation method of the artificial antioxidant enzyme material comprises the following steps: dissolving an aldehyde monomer and an amine monomer in a reaction solvent, performing thermal polymerization under the action of an acid catalyst, and then loading interlayer coordination metal, and further defining an aldehyde monomer and an amine monomer as well as an interlayer coordination metal. According to the present invention, the treatment-level oxidation resistance can be achieved through the space-optimized catalysis center and the fully-conjugated frame structure, and the innovative strategy is provided for solving the inflammation-driven tissue damage through the precise oxidation-reduction regulation and the immune microenvironment engineering.

The invention relates to a combined traditional Chinese medicine composition for treating tumors by improving a tumor immune microenvironment and application thereof. The combined traditional Chinese medicine composition comprises rhein and hesperidin. Rheinic acid and hesperidin have stronger antitumor activity than single components in combined application, and the combined pharmaceutical composition can significantly inhibit the growth of cancer-related fibroblasts, reduce CCL2 secreted by cancer-related adipocytes and increase infiltration of CD8 + T cells at tumor sites, so that the growth of tumors is significantly inhibited, and the antitumor effect is improved. And a new method and thought are provided for combined treatment of cancers.

The invention provides a preparation method for preparing hydrogel in a water phase and application of the hydrogel in tumor immune microenvironment evaluation ultrasonic detection, and relates to the technical field of organic synthesis. The preparation method comprises the following steps that chitosan is dissolved in water, a chitosan solution with a first preset concentration is prepared, and chitosan has carboxyl groups and amino groups; dissolving a condensing agent in water to prepare a reaction solution with a second preset concentration; mixing the chitosan solution with the reaction solution, adding glacial acetic acid, and reacting for preset time at preset temperature and preset pressure to obtain a mixed solution; carrying out dialysis treatment on the mixed solution to obtain hydrogel; wherein the ratio of the first preset concentration to the second preset concentration ranges from 10: 1 to 1: 1. According to the invention, the hydrogel with amido bonds is prepared in a water phase, and the hydrogel has the characteristics of low toxicity, good biocompatibility and good biodegradability.