A lab-on-a-
chip (LOC) device for
pathogen detection,
genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte
dialysis section for dividing the sample into a leukocyte
stream, and a
pathogen and erythrocyte
stream containing cells and sample constituents smaller than a first size threshold, a
pathogen dialysis section for dividing the pathogen and erythrocyte
stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte
lysis section downstream of the leukocyte
dialysis section for lysing the leukocytes with a
lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte
lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis
reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, an
erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis
reagent to release proteins therein, a first leukocyte
nucleic acid amplification section downstream of the leukocyte incubation section for amplifying
nucleic acid sequences, a second leukocyte
nucleic acid amplification section downstream of the first leukocyte nucleic acid amplification section for amplifying nucleic acid sequences, a first pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, and, a second pathogen nucleic acid amplification section downstream of the first pathogen nucleic acid amplification for amplifying nucleic acid sequences, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the leukocyte incubation section, the pathogen incubation section, the first and second leukocyte nucleic acid amplification sections and the first and second pathogen nucleic acid amplification sections are all supported on the supporting substrate.