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Mammal blood genome DNA extraction kit and method for extracting mammal blood genome DNA

A mammal and genome technology, applied in the biological field, can solve problems such as long time, yield, and unsatisfactory quality, and achieve the effects of short operation time, simple and efficient formula ingredients, and low price

Inactive Publication Date: 2014-07-02
江苏佰龄全基因生物医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this purification method also requires multiple resuspension operations, resulting in a longer time
[0013] Simultaneously, other DNA extraction methods involved in published patents have unsatisfactory yield and quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The mammalian blood genomic DNA extraction kit of this embodiment is composed of the following components: erythrocyte lysate, leukocyte lysate, protein precipitation solution and DNA precipitation solution. The components of the erythrocyte lysate and the final concentrations of each component are as follows: Tris 10mM, NH 4 Cl 100mM, EDTA disodium salt 5mM; pH 7.6; leukocyte lysate composition and final concentration of each component are as follows: Tris 10mM, EDTA disodium salt 5mM, SDS 0.1% (w / v), proteinase K 0.5g / L, The pH is 7.6; the composition and final concentration of protein precipitation solution are as follows: NaCl 5M; the composition and final concentration of each component of DNA precipitation solution are as follows: NaAC 1M, isopropanol 50% (v / v).

[0061] The preparation method of each component in the kit is as follows:

[0062] The preparation method of erythrocyte lysate is as follows: weigh 1.21g (10mmol) of Tris, NH 4 Cl 5.35g (100mmol), EDTA...

Embodiment 2

[0084] The mammalian blood genomic DNA extraction kit of this embodiment is composed of the following components: red blood cell lysate (RLB), white blood cell lysate (TB), protein precipitation solution and DNA precipitation solution, the components of red blood cell lysate and the final concentration of each component As follows: Tris 50mM, NH 4 Cl 200mM, EDTA disodium salt 50mM; pH 7.8; leukocyte lysate composition and the final concentration of each component are as follows: Tris 50mM, EDTA disodium salt 50mM, SDS 1% (w / v), proteinase K 2g / L, pH The composition and final concentration of protein precipitation solution are as follows: NaCl 5.5M; the composition and final concentration of each component of DNA precipitation solution are as follows: NaAC 2M, isopropanol 60% (v / v).

[0085] The preparation method of each component in the kit is as follows:

[0086] The preparation method of erythrocyte lysate is as follows: weigh 6.05g (50mmol) of Tris, NH 4 Cl 10.7g (200mmo...

Embodiment 3

[0107] The mammalian blood genomic DNA extraction kit of this embodiment is composed of the following components: red blood cell lysate (RLB), white blood cell lysate (TB), protein precipitation solution and DNA precipitation solution, the components of red blood cell lysate and the final concentration of each component As follows: Tris 150mM, NH 4 Cl 300mM, EDTA disodium salt 100mM; pH 8.0; leukocyte lysate composition and the final concentration of each component are as follows: Tris 150mM, EDTA disodium salt 100mM, SDS 2% (w / v), proteinase K 4g / L, pH The composition and final concentration of protein precipitation solution are as follows: NaCl 6M; the composition and final concentration of each component of DNA precipitation solution are as follows: NaAC 3M, isopropanol 70% (v / v).

[0108] The preparation method of each component in the kit is as follows:

[0109] The preparation method of erythrocyte lysate is: weigh Tris 18.17g (150mmol), NH 4 Cl 16.05g (300mmol), EDTA ...

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PUM

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Abstract

The invention provides a mammal blood genome DNA extraction kit which is composed of an erythrocyte lysis buffer, a leukocyte lysis buffer, a protein precipitation liquid and DNA precipitation liquid. Compared with the prior art, the mammal blood genome DNA extraction kit has the following technical effects: (1) no organic reagent is used, and the mammal blood genome DNA extraction kit is friendly to the environment and harmless to a human body; (2) DNA with relatively high concentration and purity can be obtained, and stretches of DNA are preserved; (3) one-step removing method is used, and is simple to operate and applicable to a wide crowd; (4) the operation time is short, a DNA sample needed by an experiment can be obtained in a short time, so that the efficiency of the experiment is greatly improved; and (5) the cost is low and the price is low.

Description

technical field [0001] The invention relates to a mammalian blood genomic DNA extraction kit and a method for extracting mammalian blood genomic DNA, belonging to the field of biotechnology. Background technique [0002] DNA extraction is the basic technology of molecular biology research, and a variety of methods for extracting DNA from blood samples have been developed. There are a lot of proteins and salts in the blood, and their properties are very similar to those of nucleic acids. In the process of blood sample DNA extraction, improper handling is easy to co-precipitate with DNA, and it is difficult to separate, which will greatly affect the quality of blood sample DNA extraction. [0003] In order to reduce or eliminate the influence of proteins and salts on DNA extraction from blood samples, organic reagents are often used in the existing DNA extraction process from blood samples, and multi-step operations are required to remove proteins and salts as much as possi...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 潘海波邢楠楠谢阳华琴
Owner 江苏佰龄全基因生物医学技术有限公司
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