32results about How to "Cracking is effective" patented technology

The invention provides a lysis solution for extracting nucleic acid through a magnetic bead method. The lysis solution is prepared from 0.2-0.4 N sodium hydroxide, 0.3-0.6 M potassium chloride, 0.01-0.05% sodium n-lauroyl sarcosinate, 5mM EDTA, 0.3-0.6 M Tris-HCL and 1-2% triton X-100. The lysis solution for extracting nucleic acid through the magnetic bead method can fully and effectively lyse cells to accelerate the lysis progress and also can protect nucleic acid against oxidation to prevent formation of DNA dimmers. Besides, the lysis solution is stable in property and free of influences of seasons, temperature, saline ion concentration and the like and can carry out nucleic acid adsorption by fully cooperating with magnetic beads to achieve the optimal nucleic acid extraction effect. The scrubbing solution for extracting nucleic acid through the magnetic bead method can effectively remove residual impurities and avoid loss of nucleic acid, and a small quantity of the residual scrubbing solution does not influence the PCR application effect, so that the accuracy of detection results is guaranteed.

The invention provides an erythrocyte lysis buffer. The erythrocyte lysis buffer comprises the following components: 4.5-5.5 mmol / L of sodium chloride, 0.8-1.3% (v / v) of triton 100, 300-340mmol / L of glucose and 0.008-0.012 mol / L of trishydroxymethyl aminomethane. The erythrocyte lysis buffer can effectively lyse the erythrocyte in a whole blood sample, separate and enrich leucocyte in the blood, and facilitate the subsequent nucleic acid extraction operation of the leucocyte, so as to obtain the leucocyte DNA in the whole blood sample and the DNA of virus in the leucocyte.

The invention discloses a lysate, nucleic acid extraction kit, and nucleic acid extraction method for nucleic acid extraction. The lysate for the nucleic acid extraction is prepared from 0.5 to 2M ofsodium salt, 2 to 5M of guanidine salt, 1 to 5mM of a metal ion complexing agent, 0.25% to 1% of a nonionic surfactant, and 1% to 3% of PEG, and the molecular weight of the PEG is 6000 to 10000 Da, preferably 8000 Da. The lysate has a reasonable ratio, cells can be fully and effectively lysed, the lysis efficiency is high, so that the nucleic acids in the cells can be fully released, and the nucleic acids with higher concentration and purity can be obtained, and the quality and extraction efficiency of the nucleic acids are improved advantageously; and according to the lysate and the kit, digestion and removal of protein can be conducted without the aid of proteinase K during the entire nucleic acid extraction process, alcohols are not usedfor precipitate the nucleic acids,the obtained nucleic acids have high purity, and fragments are complete.

The invention provides a mammal blood genome DNA extraction kit which is composed of an erythrocyte lysis buffer, a leukocyte lysis buffer, a protein precipitation liquid and DNA precipitation liquid. Compared with the prior art, the mammal blood genome DNA extraction kit has the following technical effects: (1) no organic reagent is used, and the mammal blood genome DNA extraction kit is friendly to the environment and harmless to a human body; (2) DNA with relatively high concentration and purity can be obtained, and stretches of DNA are preserved; (3) one-step removing method is used, and is simple to operate and applicable to a wide crowd; (4) the operation time is short, a DNA sample needed by an experiment can be obtained in a short time, so that the efficiency of the experiment is greatly improved; and (5) the cost is low and the price is low.

The invention discloses high-mixing-amount rubber powder-SBS composite modified asphalt and a preparation method thereof, and belongs to the technical field of road modified asphalt materials. The composite modified asphalt is prepared from, in parts by weight, 100 parts of matrix asphalt, 30-50 parts of waste tire rubber powder, 1-4 parts of SBS, 1-3 parts of a rubber powder cracking agent and 1-6 parts of aromatic oil, wherein the rubber powder cracking agent is ferric oxide. The preparation method mainly comprises the steps of raw material dehydration, modifier pre-activation, pre-shearing, formal shearing and heat preservation development. According to the high-mixing-amount rubber powder-SBS composite modified asphalt provided by the invention, the mixing amount of the rubber powder in the composite modified asphalt is increased, the modification effect of the rubber powder and the SBS is exerted to the maximum extent, the performance of the asphalt is further improved, and the composite modified asphalt has good high and low temperature performance, elastic recovery and storage stability.

The invention relates to a novel saliva preserving fluid comprising the following components: 1-10mmol / L of EDTA, 1-50mmol / L of Tris-HCl, 10-100 mmol / L of sodium chloride; 0.1-3% (w / v) of sodium sorbate, 0.1-3% (w / v) of sodium diacetate, 0.01-0.1% (v / v) of proclin 300, 0.1-1% (v / v) of TWEEN20, 0.1-1% (w / v) of NP40 and 0.1-2% (w / v) of SDS. The novel saliva preserving fluid is used for preservingsaliva samples, can effectively inhibit nuclease activity, prevent microbial pollution, guarantee DNA fragment integrity and purity, simplify pretreatment steps, is suitable for centrifuge-free and low-temperature environments, can guarantee transport stability of samples among different places, and is suitable for molecular epidemiological large-scale population investigation and research.

The invention relates to a waste tyre cracking process and device thereof. The process includes steps of cleaning tyre, cracking tyre by electrical energy heating, and reclaiming combustible gas, mixed oil, steel wire and crude carbon black. The device used for cracking process includes cracking furnace, condenser and control cabinet. The method reduces correlative devices such as burning equipment, burning chamber and smoke discharging pipe by using electrical energy as heating source; depresses energy loss to lowest by putting electrothermal heater in the cracking furnace; and is environment friendly due to not having smoke, noise, leakage, sewage, waste gas and castoff in cracking process.

The invention discloses a nucleic acid extraction method and a kit for nucleic acid extraction, and relates to the technical field of nucleic acid extraction.The extraction method comprises the steps that a sample is split through a lysis solution, and the lysis solution comprises strong ionic chaotropic salt with the working concentration being 0.5-6 M, a surfactant with the working concentration being 0.5-10 v / v% and an alcoholic solution with the working concentration being 10-50 v / v%; the lysate can effectively and stably lyse a sample under the condition that protease K is not needed, heating is not needed in the lysing process, effective extraction of nucleic acid can be completed under the normal-temperature reaction condition, in addition, magnetic beads are added in the sample lysing process in the extraction method, lysing and combining are completed in one step, the extraction quality is guaranteed, and meanwhile the extraction efficiency is improved, and the operation process is simplified.

The invention provides an extraction method of a microbe DNA for high-throughput sequencing in a sample and belongs to the field of a genome DNA extraction technology. According to the invention, DNA of a microbe in a sample is extracted by a SDS method; an inhibitor is removed after precipitation by the use of a sodium chloride solution and a aluminum ammonium sulfate solution, and then a supernatant is taken; and separation and purification are carried out by a silica gel film-column to obtain soil microbe genome DNA for high-throughput sequencing. The aluminum ammonium sulfate solution is prepared from 50-200 mM of Al2(SO4)3 and 50-200 mM of (NH4)2SO4 according to volume ratio of 1:1. The sample's microbe genome DNA extracted by the above method has high purity, good universality and fast extraction speed, can be directly used in a downstream high-throughput sequencing experiment and has a good application prospect.

The invention discloses a method for quickly extracting plasmodium DNA in a high through-put mode and application of the method. Particularly, the template extracting method comprises the steps that release, enrichment, separation and purification of DNA are conducted through alkaline lysis and magnetic beads. The method is simple and fast in operation, low in cost, high in through-put, low in contamination risk, high in quality of the extracted DNA, and capable of achieving automation and being widely used in molecular detection, the detection through-put and the operation convenience can be remarkably improved, the false positive rate of detection is obviously reduced, and the detection sensitivity and accuracy and the detection efficiency are improved. The visible and automatic sample diagnosis technology that extraction and detection of a mass of DNA on a perforated plate are integrated can be achieved through series connection of an automatic nucleic acid extractor and a PCR instrument and the PCR technology which is visible in optimizing result. The problems that in an existing plasmodium detection technology, the preparation of the target DNA is complex, wastes time, is low in through-put and the like are solved, and the method has a wide application prospect.

The invention discloses a method for extracting nucleic acid from a paraffin-embedded tissue section. The method includes the following steps: S1, performing paraffin embedding slicing; S2, performingcracking, taking out a standby section tissue in S1, adding novel lysate into the section tissue, the volume ratio of the section tissue to the novel lysate being 1 : 50-100, performing centrifugal layering after performing mixing and uniform shaking for 10-15 min under a temperature of 65-75 DEG C, performing standing after layering, and taking an intermediate layer after standing so that mixedliquor can be obtained; S3, performing combination; S4, performing washing; and S5, performing elution. Cells can be fully and effectively cracked by using the novel lysate to perform the cracking ofnucleic acid on the section tissue, so that a cracking process can be accelerated, the nucleic acid can be protected from oxidizing, and the formation of DNA self-dimer can be avoided; and the methodhas the characteristics of being short in using time, high in extraction efficiency and high in extraction quality, so that the method is suitable for being popularized and used.

The invention relates to the expression of reforming bovine insulin ribonucleic acid hydrolytic enzyme A (RNaseA) and a depurant method, the genetic expression and the albumen purification technology in modern biology are adopted to perform an improvement to the preparation method of the reforming RNaseA, and the invention provides the preparation method of the reforming RNaseA which can be widely popular in biological medicine, is safe and feasible, and can greatly reduce the manufacturing cost.

The invention provides an extraction method of metagenome of microbes in seawater. According to the invention, the special sample, seawater, undergoes saline ions removal treatment, double-membrane filtration and recovery processing. The invention aims to provide an efficient and rapid method for extracting metagenome of microbes in seawater. The method comprises the following specific steps: removal of impurities in seawater, cracking of microbes in seawater, dissociation and adsorption of DNA and DNA elution. Interference of many saline ions in seawater can be effectively eliminated; and microbial cells in seawater are fully cracked to fully release DNA. According to the DNA extracted by the method, A260 / 280 ratio of the DNA is maintained between 1.8 and 2.0. The obtained DNA has good quality and high purity, can meet requirements of gene library construction and qPCR and high-throughput sequencing, and provides good foundation for the research on heritable variation diversity of microbial genomes.

The invention discloses a lysate for nucleic acid extraction, a nucleic acid extraction kit and a nucleic acid extraction method. The nucleic acid extraction lysate of the present invention comprises 0.5-2M sodium salt, 2-5M guanidinium salt, 1-5mM metal ion complexing agent, 0.25%-1% non-ionic surfactant and 1%-3% PEG, wherein the The molecular weight of the PEG is 6000-10000Da, preferably 8000Da. The lysate of the present invention has a reasonable proportion, can fully and effectively lyse cells, and has high lysis efficiency, so that nucleic acids in cells can be fully released, and nucleic acids with higher concentration and purity can be obtained, which is conducive to improving the quality and extraction efficiency of nucleic acids; and the present invention The inventive lysate and kit do not require proteinase K to digest and remove proteins during the entire nucleic acid extraction process, and do not use alcohols to precipitate nucleic acids, so the obtained nucleic acids have high purity and complete fragments.

The invention provides polypeptide as well as a preparation method and an application thereof in inhabitation of HIV. The polypeptide comprises an amino acid sequence shown in X-W / A-E / A-A / E-L / A-X-Y / A-L / A-W / A-N / A-L / A-L / A-Q / A-Y / A-W / A. The invention also provides nucleic acid coding the polypeptide, a carrier containing the nucleic acid, a host, a preparation or use method and an application. The invention further provides fusion protein, a pharmaceutical composition or conjugated containing the polypeptide.

The invention provides a thermal cracking treatment device and method for radioactive waste resin, and belongs to the technical field of nuclear industry. The thermal cracking treatment device comprises a microwave resonance and heating system 1, a microwave generation and control system 2, a thermal cracking gas treatment system 3, a nitrogen input system 4 and a residual carbon collection cavity5. According to the method, the radioactive waste resin is heated through microwaves, the method has the advantages of being safe, clean and efficient, dehydration and thermal cracking of the radioactive waste resin can be achieved within a short time, on one hand, organic matters in the resin are cracked into gases, the size of the waste resin is greatly reduced, and radionuclide is enriched inresidual carbon; on the other hand, the residual carbon obtained after resin pyrolysis is a microwave absorbing material, and the heating efficiency in the microwave pyrolysis process can be effectively improved. According to the present invention, the thermal cracking gas treatment system 3 can treat the gas generated by thermal cracking so as to avoid the emission of radionuclide-containing solid particles and harmful gases.

The invention discloses combustible gas wet type purification equipment in the new energy field. The combustible gas wet type purification equipment structurally comprises a gas inlet pipeline, a dustremoval box, a connecting pipeline, a tar splitting reactor, a first-level gas outlet pipeline, a purification tower, a control box, a gas outlet and a second-level gas outlet pipeline. The arrangedtar splitting reactor is combined with the purification tower, tar hard to decompose in combustible gas can be split, and purification is conducted in a spraying purification manner of the purification tower. A first spraying mechanism and a second spraying mechanism arranged in the purification tower can achieve the dual spraying purification effect. The second spraying mechanism can conduct selective spraying according to the gas inlet amount of the combustible gas, the purification best degree is guaranteed, and environment friendliness is achieved under the situation that materials are notwasted.

The invention discloses a human hair rapid lysate for trace drug detection and application thereof. The human hair rapid lyse for trace drug detection has a raw material component comprising: 50-100nM alkaline protease AH-101 , 0.5‑2% thioglycolic acid, 25‑75 mM glycine, 30‑60 mM tris hydrochloride buffer, 40‑80 mM sodium chloride‑sodium hydroxide buffer, and 0.5‑1.5% polyethylene glycol octane phenyl phenyl ether (Triton X-100); wherein, nM is nanomoles per liter, mM is millimoles per liter, and % is volume percent. The present invention can quickly extract trace drug small molecules from human hair samples, has high release rate of drugs, little influence of impurities, non-toxic and pollution; Quickly, the whole cracking time only needs 90 seconds, so that the drug molecules are effectively released, compared with the cracking method in the prior art, the operation time is greatly shortened, and the authenticity and reliability of the experimental results are guaranteed.

The invention provides a cervical cell lysis kit and a cell lysis method, belonging to the technical field of cell lysis. The kit includes a lysis buffer and proteinase K. The lysis buffer comprises the following components in concentration: 10-500mM of Tris-HCl, 1-80 mM of EDTA, 0.05-2 wt% of SDS and 100-250 mM of NaCl. The cell lysis method comprises the following steps: after cleaning a cervical cell sample solution with PBS buffer, uniformly mixing the solution with lysis buffer, adding proteinase K, and digesting at 53-58 DEG C for 10-14 hours; After 30-60 s of vortex vibration, digestingthe cells at 53-58 DEG C for 1.5-2.5 h to complete cell lysis. The kit and the lysis method can fully lyse cervical cells in the sample, dissociate high-concentration and high-quality DNA, and facilitate subsequent DNA extraction and amplification.

The invention provides polypeptides, their preparation methods and uses for inhibiting HIV. The polypeptide contains X‑W / A‑E / A‑A / E‑L / A‑X‑Y / A‑L / A‑W / A‑N / A‑L / A‑L / A‑Q / A The amino acid sequence represented by ‑Y / A‑W / A. Also provided are nucleic acids encoding polypeptides, vectors, hosts, methods of making or using the nucleic acids, and uses. Fusion proteins, pharmaceutical compositions or conjugates comprising polypeptides are also provided.

The invention relates to a method for simply, conveniently and quickly detecting nucleic acid molecules in exosomes. The method comprises the following steps: cracking the exosomes in a sample to release the nucleic acid molecules of the exosomes; and performing reverse transcription reaction: after the exosomes are cracked, the nucleic acid molecules are not extracted, or after the nucleic acid molecules are extracted, reverse transcription reaction of all RNA is carried out, namely RT-For-All, so that all RNA in the exosomes is simultaneously subjected to reverse transcription in the same reaction; and a reverse transcription buffer comprising a universal adapter (e.g., miRNA Adapter) and a random primer is used in the reverse transcription reaction. The invention also provides a kit for detecting the nucleic acid molecules in the exosomes. Contained components are released by directly dissolving the exosomes, RNA and DNA do not need to be extracted, and reverse transcription of all RNA is directly carried out in the same reverse transcription reaction. Various RNA and DNA can be detected from trace exosomes.

The present invention provides a new light green balloon bacteria phage AVP, which is preserved in the China Center for Type Culture Collection, the preservation number is: CCTCC NO: M 2018155; the preservation time is: March 25, 2018; the new light green balloon bacteria phage AVP The bacteriophage AVP lysate that can lyse light green balloon bacteria, the phage has a wide lysis spectrum, not only can lyse light green balloon bacteria, but also can lyse Staphylococcus aureus and Staphylococcus epidermidis, can lyse standard strains, can also lyse Clinical strains isolated from the environment or feces of diseased animals can be used alone or in combination with other substances to provide a safe and non-toxic phage disinfecting product for disinfection and purification of the environment.

The invention discloses a rapid PCR amplification kit for yeast colonies and a method for performing PCR amplification on the yeast colonies by using the kit. The kit comprises: 10 x cell lysis buffer, 10 x PCR reaction solution, dNTP and hot-start Taq enzyme. Compared with the prior art, the kit does not need the genome extraction steps of heating, boiling, phenol / chloroform extraction, and the like, so that the toxicity of toxic substances to experimental personnel is reduced. The method has quick and simple operation and saves a great deal of time. The method directly lyses monoclonal colonies, reduces cross pollution, and avoids false positivity. By using the cell wall lysis buffer with high pH value, the cell wall of the yeast can be effectively lysed in a short time, the yeast genomecan be fully released, the pH value of the released supernatant after being mixed with the matched PCR reaction solution is within a proper range of PCR amplification, and the rapid PCR amplificationcan be realized.

The invention discloses a kit and a method for real-time fluorescence quantitative detection of hepatitis B virus (HBV) deoxyribonucleic acid (DNA) in serum. By using dodine to replace the traditionalguanidine salt and part of a decontamination agent in the lysate, the lysate can effectively lyse a serum sample containing HBV particles so as to release the HBV DNA in the serum sample; therefore,the kit and the method remarkably increases the lysis efficiency and greatly shortens the detection time.

The invention provides an extraction method of metagenome of microbes in seawater. According to the invention, the special sample, seawater, undergoes saline ions removal treatment, double-membrane filtration and recovery processing. The invention aims to provide an efficient and rapid method for extracting metagenome of microbes in seawater. The method comprises the following specific steps: removal of impurities in seawater, cracking of microbes in seawater, dissociation and adsorption of DNA and DNA elution. Interference of many saline ions in seawater can be effectively eliminated; and microbial cells in seawater are fully cracked to fully release DNA. According to the DNA extracted by the method, A260 / 280 ratio of the DNA is maintained between 1.8 and 2.0. The obtained DNA has good quality and high purity, can meet requirements of gene library construction and qPCR and high-throughput sequencing, and provides good foundation for the research on heritable variation diversity of microbial genomes.

The invention provides a thermal cracking treatment device and method for radioactive waste resin, belonging to the technical field of nuclear industry. The device provided by the present invention includes a microwave resonance and heating system 1 , a microwave generation and control system 2 , a pyrolysis gas treatment system 3 , a nitrogen gas input system 4 and a carbon residue collection chamber 5 . The present invention uses microwaves to heat radioactive waste resin, which has the characteristics of safety, cleanliness and high efficiency, and can realize dehydration and pyrolysis of radioactive waste resin in a relatively short period of time. The volume of the waste resin and enrich the radionuclide in the residual carbon; on the other hand, the residual carbon after resin pyrolysis is also a microwave absorbing material, which can effectively improve the heating efficiency in the microwave pyrolysis process. In the present invention, the pyrolysis gas treatment system 3 can process the gas generated by pyrolysis, avoiding the emission of solid particles containing radionuclides and harmful gases.

The invention relates to a method for preparing clean combustible gas and active coke powder through coal pyrolysis. The method includes the following steps: 1, preparing coal dust: processing coal dust to prepare coal dust with required fineness; 2, carrying out pyrolysis: sending the coal dust obtained in step 1 into a pyrolysis furnace, and carrying out instant cracking in anaerobic closed environment to generate active coke powder and clean combustible gas; and 3, separating and recovering: discharging the active coke powder and the clean combustible gas from the pyrolysis furnace, and recovering. The whole process is instantly completed in a closed loop system without water, coal combustion or loss, or pollution emission, so the efficient clean conversion of coal is realized, and the energy utilization efficiency is improved.

The invention discloses a real-time fluorescent quantitative detection kit of HBV DNA in serum and a detection method thereof. In the lysate used, Duofruit is used instead of traditional guanidinium salt and a part of detergent to effectively detect HBV-containing viruses. The serum sample of the particles is lysed to release the HBV DNA, which significantly improves the lysis efficiency of the HBV virus and greatly shortens the detection time.