7 results about "Paraffin embedded tissue" patented technology

A rapid, label-free, non-destructive imaging method and system (100) for unprocessed biological tissue, which is based on microscopy with ultraviolet single plane illumination (MUSI). The present system (100) or method employs a selective two-axis planar illumination configuration, which decouples the illumination from the detection path, and utilize the intrinsic fluorescence of certain endogenous fluorophores from biological tissues as a natural source compared to deep UV illumination sources. In contrast to images produced by clinical standard methods (i.e. H&E staining of formalin-fixed and paraffin-embedded tissues), images generated by MUSI reveal comparable or even better diagnostic features, providing clinicians, pathologists, and surgeons with greater potential as post-operative and intra-operative diagnostic tools.

The invention discloses a safe, non-toxic and environment-friendly dewaxing liquid for dewaxing a paraffin-embedded tissue slice, and relates to the technical field of biological tissue slice preparation. The specific preparation method comprises the following steps: firstly, weighing Isoparl H, Isoparl M and Isoparl L in a reaction container, uniformly mixing, then weighing amyl alcohol and hexyl alcohol, uniformly mixing, finally adding castor oil, and uniformly mixing, and the components comprise, by weight, 20-30% of Isoparl H, 30-50% of Isoparl M, 10-20% of Isoparl L, 1-10% of castor oil, 1-5% of amyl alcohol and 2-10% of hexyl alcohol. The prepared dewaxing liquid is safe, non-toxic and friendly to human bodies, the health risk caused by long-term exposure when an operator uses a traditional dewaxing agent is reduced, the dewaxing efficiency is high, paraffin can be rapidly and thoroughly removed within 10-20 minutes under the condition of room temperature or slight heating (30-37 DEG C), the tissue form is well kept, high-temperature treatment is not needed, the dewaxing liquid is friendly to the environment, the formula is stable, and the dewaxing liquid is suitable for industrial production. The method is suitable for large-scale production, popularization and application.

Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

The invention discloses a slice treating fluid and method for preserving antigen activity in a slice, and belongs to the technical field of immunodetection. Wherein the section is obtained by utilizing a pathological section preparation technology, and the section treatment liquid comprises a pentacyclic triterpenoid compound. By utilizing the section treating fluid disclosed by the invention, the antigen activity of the paraffin-embedded tissue section can be kept for at least 9 months when the paraffin-embedded tissue section is stored at normal temperature, and the storage life of the antigen activity in the cell slide at normal temperature can be effectively prolonged.

The invention relates to a tool (1) for collecting a sample, suitable for molecular analysis, comprising a tube (2), a rod (3) comprising a rod body (3-1), an ejection end and a gripping end (3-2) extending out of the first end of the tube (2), so as to allow a movement of the rod (3) along a longitudinal axis of the tube (2), the sampling tool (1) being arranged so as to pass from a sampling configuration in which the ejection end of the rod body (3-1) partially occupies the second end (2-2), to an ejection configuration in which the ejection end of the rod body (3-1) extends out from the second end (2-2) of the tube (2). The invention further relates to a device (6) and a method for collecting an associated sample, suitable for molecular analysis.

The application discloses a paraffin-embedded tissue section sample scraping device, which comprises a machine box, a section conveying device, a section scraping knife scraping device and a controller, the section conveying device is fixedly arranged in the machine box, the section conveying device comprises a section clamp and a section clamp conveying piece, the section clamp is used for placing a paraffin-embedded tissue section, and the section clamp is fixedly arranged on the section clamp conveying piece; the section scraping knife scraping device is horizontally and slidably arranged in the machine box, the section scraping knife scraping device comprises a section scraping knife driving piece and a section scraping knife, the section scraping knife driving piece is drivingly connected with the section scraping knife and is used for driving the section scraping knife to move up and down so as to scrape the paraffin-embedded tissue section on the section clamp; and the controller is electrically connected with the section conveying device and the section scraping knife scraping device, so as to control the section conveying device and the section scraping knife scraping device to work. The device can automatically scrape sections, reduces the biological safety risk caused by manual sectioning, and improves sectioning efficiency.

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.