11 results about "Paraffin embedded tissue" patented technology

The invention discloses a differentiated thyroid cancer lung metastasis risk prediction model construction method and system. 4D Label-free quantitative proteomics analysis and PRM and IHC dual verification are carried out on primary focus paraffin embedded tissue samples of lung metastatic DTC patients and non-lung metastatic DTC patients, and it is found that multiple key differential expression proteins DEPs may become potential biomarkers for predicting the DTC lung metastasis risk. In addition, the sample size is enlarged, three single and combined DTC lung metastasis risk prediction models are respectively constructed based on clinical features and key DEPs expression, then internal verification is performed on the efficiency of the three fitted models, and the optimal model is taken to construct Nomogram. According to the method, help is provided for risk stratification of lung metastasis of DTC patients, and an important basis is provided for formulating clinical individualized treatment schemes and follow-up visit strategies for high-risk patients.

Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.

A rapid, label-free, non-destructive imaging method and system (100) for unprocessed biological tissue, which is based on microscopy with ultraviolet single plane illumination (MUSI). The present system (100) or method employs a selective two-axis planar illumination configuration, which decouples the illumination from the detection path, and utilize the intrinsic fluorescence of certain endogenous fluorophores from biological tissues as a natural source compared to deep UV illumination sources. In contrast to images produced by clinical standard methods (i.e. H&E staining of formalin-fixed and paraffin-embedded tissues), images generated by MUSI reveal comparable or even better diagnostic features, providing clinicians, pathologists, and surgeons with greater potential as post-operative and intra-operative diagnostic tools.

The invention discloses a safe, non-toxic and environment-friendly dewaxing liquid for dewaxing a paraffin-embedded tissue slice, and relates to the technical field of biological tissue slice preparation. The specific preparation method comprises the following steps: firstly, weighing Isoparl H, Isoparl M and Isoparl L in a reaction container, uniformly mixing, then weighing amyl alcohol and hexyl alcohol, uniformly mixing, finally adding castor oil, and uniformly mixing, and the components comprise, by weight, 20-30% of Isoparl H, 30-50% of Isoparl M, 10-20% of Isoparl L, 1-10% of castor oil, 1-5% of amyl alcohol and 2-10% of hexyl alcohol. The prepared dewaxing liquid is safe, non-toxic and friendly to human bodies, the health risk caused by long-term exposure when an operator uses a traditional dewaxing agent is reduced, the dewaxing efficiency is high, paraffin can be rapidly and thoroughly removed within 10-20 minutes under the condition of room temperature or slight heating (30-37 DEG C), the tissue form is well kept, high-temperature treatment is not needed, the dewaxing liquid is friendly to the environment, the formula is stable, and the dewaxing liquid is suitable for industrial production. The method is suitable for large-scale production, popularization and application.

Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

The invention discloses a slice treating fluid and method for preserving antigen activity in a slice, and belongs to the technical field of immunodetection. Wherein the section is obtained by utilizing a pathological section preparation technology, and the section treatment liquid comprises a pentacyclic triterpenoid compound. By utilizing the section treating fluid disclosed by the invention, the antigen activity of the paraffin-embedded tissue section can be kept for at least 9 months when the paraffin-embedded tissue section is stored at normal temperature, and the storage life of the antigen activity in the cell slide at normal temperature can be effectively prolonged.

The utility model provides an automatic demoulding device for pathological paraffin embedding tissues, which comprises a dewaxing table, the upper end of the dewaxing table is sunken downwards to form a dewaxing groove matched with the shape of a bottom membrane of an embedding box, an electric heating sheet is arranged on the outer wall of the dewaxing groove in the dewaxing table, a support column is upwards arranged at the upper end of the dewaxing table, and the lower end of the dewaxing table is connected with the support column. A mounting table located above the dewaxing groove is horizontally arranged at the upper end of the supporting column, a telescopic motor is downwards mounted at the upper end of the mounting table, a connecting strip is arranged at the lower end of a telescopic shaft of the telescopic motor, clamping arms capable of vertically rotating are rotationally connected to the two ends of the connecting strip, and a torsional spring is connected between each clamping arm and the rotating part of the connecting strip; each torsion spring drives the corresponding clamping arm to rotate outwards, an electromagnet is installed at the lower end of the connecting strip, and the inner side of each clamping arm is connected with an iron sheet matched with the electromagnet through a connecting block. The automatic dewaxing device can replace manual work to carry out automatic dewaxing, is convenient and fast, and is not easy to damage a wax block.

The invention relates to a tool (1) for collecting a sample, suitable for molecular analysis, comprising a tube (2), a rod (3) comprising a rod body (3-1), an ejection end and a gripping end (3-2) extending out of the first end of the tube (2), so as to allow a movement of the rod (3) along a longitudinal axis of the tube (2), the sampling tool (1) being arranged so as to pass from a sampling configuration in which the ejection end of the rod body (3-1) partially occupies the second end (2-2), to an ejection configuration in which the ejection end of the rod body (3-1) extends out from the second end (2-2) of the tube (2). The invention further relates to a device (6) and a method for collecting an associated sample, suitable for molecular analysis.

The application discloses a paraffin-embedded tissue section sample scraping device, which comprises a machine box, a section conveying device, a section scraping knife scraping device and a controller, the section conveying device is fixedly arranged in the machine box, the section conveying device comprises a section clamp and a section clamp conveying piece, the section clamp is used for placing a paraffin-embedded tissue section, and the section clamp is fixedly arranged on the section clamp conveying piece; the section scraping knife scraping device is horizontally and slidably arranged in the machine box, the section scraping knife scraping device comprises a section scraping knife driving piece and a section scraping knife, the section scraping knife driving piece is drivingly connected with the section scraping knife and is used for driving the section scraping knife to move up and down so as to scrape the paraffin-embedded tissue section on the section clamp; and the controller is electrically connected with the section conveying device and the section scraping knife scraping device, so as to control the section conveying device and the section scraping knife scraping device to work. The device can automatically scrape sections, reduces the biological safety risk caused by manual sectioning, and improves sectioning efficiency.

The invention discloses a solid-phase chemical enzyme method for enriching and identifying glycosylated DNA (Deoxyribose Nucleic Acid) and sites thereof. The efficient enrichment of glycosylated deoxynuclear nucleic acid (DNA) fragments is realized through the condensation reaction of aldehyde groups and hydrazide and the specific action of enzyme. The method aims at filling the blank of the prior art, and further reveals the potential effect of DNA glycosylation in the life process and the disease mechanism through deep research on DNA glycosylation. The method can be simultaneously suitable for various types of biological samples, including paraffin embedded tissues, fresh tissues and blood / cell samples, has wide applicability, and can be used for analyzing DNA glycosylation sites and sequences in multiple steps. According to the method, complete DNA glycosylation site information can be obtained, an important technical means is provided for researching DNA epigenetic modification, and the method has important application value in the fields of tumor marker screening, disease diagnosis, drug research and development and the like.

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.