32 results about "Acid release" patented technology

The invention relates to the technical field of pathogenic microorganism detection, in particular to a pathogenic microorganism detection system based on nanopore sequencing, which comprises: a microflow sample introduction module, which uses nanopore sequencing, regulates and controls a microorganism liquid channel and adjusts the opening of a sample introduction valve at the same time, setting the flow rate of the trace liquid and comparing the ion concentration of the conductive pore channel with the pH value of the solution by using a sensor. According to the invention, liquid channel regulation and control are carried out through nanopore sequencing, and accurate control of a fluid environment is realized by combining opening adjustment of a sample introduction valve, flow velocity setting and comparison of ion concentration and pH value of a conductive pore channel, so that a microbial sample is ensured to enter a sequencing system under stable physical and chemical conditions, and the stability of sequencing data is improved. In the cell lysis process, the nucleic acid release rate is adjusted through temperature monitoring, and the particle removal operation is combined, so that the nucleic acid component in the sample is more uniform, and the reliability of subsequent detection is improved.

The invention relates to the technical field of organic solid acids, in particular to a preparation method of a controllable release type organic solid acid, which comprises the following steps: (1) preparing a pH and temperature dual-sensitive polymer carrier; (2) loading an acid precursor onto the polymer support; (3) carrying out surface modification on the nano silicon dioxide; and (4) mixing the polymer loaded with the acid precursor with the modified nano silicon dioxide, and preparing the composite microspheres by adopting a spray drying method. The pH-sensitive polymer PDEAEMA and the temperature-sensitive polymer PNIPAM are combined, so that the dual response to the pH and temperature of the reservoir is realized. A tertiary amine group in a PDEAEMA molecule is protonated in a low-pH environment, so that a polymer chain is stretched, and release of an acid precursor is promoted. Meanwhile, the PNIPAM is subjected to phase change when the LCST is higher than the LCST (about 32 DEG C) of the PNIPAM, the hydrophilic property is converted into the hydrophobic property, a polymer network is shrunk, and accurate control over acid release is achieved.

A fatty acid releasing contact lens and a method for making the same are described. The fatty acid releasing contact lens comprises a glycerophospholipid loaded into a polymeric lens body and sustains the release of C12-C26 fatty acids after 1 hour in a release medium comprising an sPLA2 enzyme solution. The fatty acid releasing contact lens can be comfortably worn by a contact lens wearer, can increase comfortable lens wear time, and / or can reduce lens sensation events in a contact lens wearer.

The present application relates to a novel long-acting solid coated slow-release acid and a preparation method. The solid coated slow-release acid has a core-shell structure, the shell layer comprises a polymer, and the core comprises an acid former; the acid former is at least one selected from chlorinated aromatic hydrocarbons, chlorinated acid esters and acid chlorides, and the polymer is at least one selected from polyesters, poly(meth)acrylates, epoxy resins, polyolefins, polyacrylonitriles, polyimides, polyvinyl alcohols. The solid coated slow-release acid has the advantages of small environmental pollution, long acid release time, excellent deep penetration performance, high acid generating efficiency, easy storage and transportation, good slow-release performance and simple preparation.

A composite element (6), e.g. a wind turbine blade, comprising a stack (20) of at least two fibre mats (13) of oriented fibres (18) embedded in a matrix of cured acid breakable resin (14) is disclosed. The fibres (18) of each fibre mat (13) are fixated relative to each other by means of first fixation means (19) made from a first material, and the at least two fibre mats (13) are fixated relative to each other by means of second fixation means (16) made from a second material. The second material of the second fixation means (16) is an acid breakable material. The composite element (6) is highly recyclable by exposing the composite element (6) to an acid containing release agent (11). Furthermore, a method for manufacturing such a composite element (6) and a method for disassembling such a composite element (6) are disclosed.

The invention discloses a universal sample type nucleic acid releasing agent and application thereof. The nucleic acid releasing agent is prepared from the following components in molar concentration: 20 to 100 mM of Tris-HCl, 30 to 500 mM of sodium chloride, 30 to 200 mM of ammonium sulfate, 1 to 6 mM of EDTA (Ethylene Diamine Tetraacetic Acid), 1 to 8 mM of a reducing agent, 0.1 to 0.2 percent of sodium dodecyl sarcosinate, 0.1 to 3 mM of Surfactin, 5 to 30 percent of Chelex-100 and 5 to 15 percent of trehalose. According to the method, various samples can be mildly split, impurities such as impure proteins and lipids can be effectively removed, the complexity of nucleic acid samples is reduced, and the method is seamlessly jointed with subsequent PCR (Polymerase Chain Reaction); the kit has double effects of sample preservation and splitting decomposition, can strongly inhibit various nuclease, and can ensure the long-term storage stability of samples.

The microfluidic chip for detecting nucleic acid of microorganisms of the present invention has a first solenoid valve that controls the on-off of the inlet and outlet of the eluent, and the eluent flows out from the outlet through the sample chamber of the sample injection chip, and enters the degassing channel through the sample inlet; the second solenoid valve controls the on-off of the outlet of the degassing channel and the inlet of the lysis pool, and the sample completes the release of nucleic acid in the lysis pool; the third solenoid valve controls the on-off of the outlet of the lysis pool and the inlet of the reaction detection pool, and the reaction detection pool is provided with a plurality of reaction chambers, and the freeze-dried powder of the LAMP-CRISPR reaction system is pre-set in the reaction chamber, and the constant temperature amplification of nucleic acid is completed in the reaction chamber. The chip card box of the present invention is fully integrated, and has low requirements for operators; a swab is used for direct sample loading, and no manual extraction process is required; the chip card box is plug-in and unplug design, and is convenient for installation and replacement.

An oleic acid-releasing contact lens is described as well as method of manufacturing the same. The oleic acid-releasing contact lens contains oleic acid releasably adhered to the polymeric lens body, and releases from 2 μg to 25 μg oleic acid after 1 hour in a release media. The oleic acid-releasing contact lens can be comfortably worn by a symptomatic contact lens wearer, and can increase the duration of comfortable lens wearing time and / or reduce lens awareness events in a symptomatic contact lens wearer.

The invention provides a method for directly amplifying pathogens in a throat swab sample, which comprises the following steps of: preparing a throat swab sample nucleic acid releasing agent from NP-40, Tris-base, EDTA (Ethylene Diamine Tetraacetic Acid), Chelex-100 and KCl, collecting the throat swab sample in normal saline during direct amplification, fully oscillating, taking 1mL of the throat swab sample, centrifuging 12,000 g of the throat swab sample for 10 minutes, discarding supernatant, retaining precipitate, adding 100 microliters of the throat swab sample nucleic acid releasing agent according to claim 1, shaking for 30-60 seconds by hand, and detecting the pathogens in the throat swab sample. And standing for 1-2 minutes to obtain a nucleic acid template for PCR (Polymerase Chain Reaction) detection. The method greatly simplifies the nucleic acid purification process of a clinical sample, greatly shortens the detection time, and has the advantages of low cost and convenience in use.

The utility model discloses an acid amount control device for adding acid into a lead-acid battery, which comprises a rack, and the rack is provided with an acid feeding box, an acid feeding pump, an acid storage tank, an acid storage tank and an acid storage tank, and is characterized in that the acid feeding box is externally connected with the acid feeding pump; the acid adding quantitative cup is installed at the bottom of the acid feeding box, the bottom of the acid adding quantitative cup is connected with a communication hose, and the communication hose is communicated with an acid adding assembly used for adding acid to the battery; the acid amount control rod is inserted into the acid adding quantitative cup and can move in the vertical direction; the communicating hose penetrates through the acid discharging opening and closing assembly, and the acid discharging opening and closing assembly can control opening and closing of the communicating hose. The acid adding device has the beneficial effects that the acid adding amount is controlled by adjusting the length of the acid amount control rod extending into the acid feeding box, the control effect of the acid adding amount is good, and quantitative acid adding is ensured.

The present disclosure relates in some aspects to methods, systems, and kits for using diphosphonic acid to promote N—O bond cleavage in a 3′ O—NH2 nucleotide in a cell or tissue sample. In some aspects, diphosphonic acid is used to promote N—O bond cleavage in a 3′ O—NH2 blocked reversible terminator during multiple cycles of a nucleic acid sequencing reaction in a cell or tissue sample. In some embodiments, the chemical reaction of the 3′ O—NH2 blocked reversible terminator with diphosphonic acid releases free alcohols and phosphoric acid derivatives, which are not damaging to the cell or tissue sample.

The invention discloses a corn softening method based on composite biochemical synergistic treatment, and relates to the technical field of food processing, and the method comprises the following steps: raw material pretreatment: soaking corn kernels in cold water at 10-15 DEG C for 10-14 hours, and carrying out salting-out treatment according to the proportion that 0.4-0.6 kg of edible salt is added into 100 kg of corn; the biological-chemical synergistic treatment comprises the following steps: a) compound alkali treatment: adding edible sodium carbonate accounting for 0.4-0.6% of the dry weight of the corn, adding food-grade baking soda accounting for 0.3-0.7% of the dry weight of the corn, mixing, and maintaining the pH value at 8.5-9.2 for 1-2 hours; b) compound enzyme treatment: 0.002%-0.005% of cellulase, 0.001%-0.003% of hemicellulase and 0.0005%-0.0015% of lignin peroxidase are synchronously added; and nicotinic acid release: carrying out alkali treatment on 0.5 jin / 100 jin of corn to destroy combined nicotinic acid, and further releasing vitamin B groups by combining microbial fermentation pine fungus spores and coprinus comatus enzyme to improve the biological accessibility.

This invention belongs to the field of extraction and separation technology, specifically involving the preparation of extracted cinnamaldehyde into microspheres for controlled release. First, a responsive silica-polymer porous particle is prepared. After cinnamaldehyde is adsorbed onto the responsive silica-polymer porous particle, the cinnamaldehyde-releasing nanoparticles are then encapsulated with modified amino-modified hydrophobic porous starch particles to form double-encapsulated cinnamaldehyde-releasing particles. Finally, a cinnamon crude oil with controlled release of cinnamaldehyde is produced. This double-encapsulated cinnamaldehyde-releasing particle has a high loading capacity and excellent controlled release effect on cinnamaldehyde. It is stable under light and high temperature and can achieve acid-induced release. Furthermore, the obtained cinnamon crude oil is close to natural cinnamon crude oil, with superior components, good biocompatibility, and good antioxidant, antibacterial, and anti-inflammatory effects.

The invention relates to the field of biological detection, in particular to a nucleic acid releasing agent and a preparation method and application thereof. The invention provides a nucleic acid releasing agent. The nucleic acid releasing agent comprises chaotropic salt, polyethylene glycol, a surfactant, an antifreeze agent, a penetrant, a metal chelating agent, an antioxidant and a buffer solution. According to the invention, the extraction-free rapid nucleic acid releasing agent is researched and developed, and an ultrasonic treatment scheme is introduced to carry out rapid pretreatment on the sample, so that the problems of tedious nucleic acid extraction process, long time consumption and low efficiency in the prior art are successfully solved, and an efficient, convenient and low-cost nucleic acid releasing and detecting scheme is provided for clinical detection and scientific research.

This invention discloses a high-temperature acid-responsive degradable microencapsulated acid for acid fracturing and its preparation method, belonging to the technical field of chemical materials for acid fracturing in oil and gas fields. The high-temperature acid-responsive degradable microencapsulated acid for acid fracturing includes an acid core and a microcapsule shell encapsulating the acid core. The microcapsule shell is made of a high-temperature acid-responsive degradable polymer material, which is polymerized from five monomers: acrylamide, tert-butyl methacrylate, 2-acrylamide-2-methylpropanesulfonic acid, diallylamine, and allyl polyoxyethylene ether. The microencapsulated acid of this invention can achieve delayed acid release and shell degradation in high-temperature acid solutions, effectively reducing reservoir damage caused by shell residue while ensuring etching capability. It also exhibits strong temperature and shear resistance, making it suitable for acid fracturing operations in high-temperature / ultra-high-temperature carbonate reservoirs.

In various embodiments novel biodegradable acid-activated acid releasing nanoparticles (acNPs) are provided that are used as a targeted strategy to manipulate lysosomal acidity and autophagy. These acNPs based, in certain embodiments, on fluorinated polyesters are degraded at pH 6.0 (pH reported in dysfunctional lysosomes), and release component acids that further lower the lysosomal pH, and thereby increasing autophagic flux and cellular function of hepatocytes under LT. The acNPs can serve as a therapeutic in restoring liv- er-diseases.

The invention belongs to the technical field of extraction and separation, and particularly relates to a method for preparing a controlled release microsphere from extracted cinnamyl aldehyde, which comprises the following steps: firstly, preparing a response type silica polymer porous particle, adsorbing cinnamyl aldehyde by the response type silica polymer porous particle, and then preparing the controlled release microsphere. And wrapping the cinnamyl aldehyde slow-release nanoparticles with modified aminated hydrophobic porous small starch particles to form double-wrapped cinnamyl aldehyde slow-release particles, and finally preparing the cinnamon crude oil capable of controllably releasing cinnamyl aldehyde. The double-coated cinnamyl aldehyde sustained-release particle is high in loading capacity, has an excellent controlled-release effect on cinnamyl aldehyde, is stable under illumination and high temperature, and can be released when encountering acid. And the prepared cinnamon crude oil is close to natural cinnamon crude oil, has better components and good biocompatibility, and has good antioxidant, antibacterial and anti-inflammatory effects.

The quantitative feeding device comprises a rotating disc, feeding assemblies and weighing sensors, the multiple feeding assemblies are arranged in the circumferential direction of the rotating disc, each feeding assembly comprises a storage hopper and a material blocking plate, a feeding opening is formed in the upper portion of each storage hopper, and a discharging opening is formed in the lower portion of each storage hopper; the striker plate is arranged at the discharge hole and is connected with the storage hopper; the multiple weighing sensors are in one-to-one correspondence with the multiple feeding assemblies, and the weighing sensors are arranged between the storage hopper and the rotating disc. Methanesulfonic acid is quantitatively weighed through the weighing sensor, then the methanesulfonic acid is transferred to a designated position through the rotating disc, then the material blocking plate moves, methanesulfonic acid in the storage hopper is released, and the steps are repeated after releasing is completed, so that quantitative feeding of methanesulfonic acid is achieved, manual participation is not needed in the whole process, and the body health of operators is effectively guaranteed.

The utility model discloses a vacuumizing assembly for acid adding of a lead-acid battery, which comprises a rack, the rack is provided with an acid adding assembly, the acid adding assembly is provided with a plurality of acid adding cups, the upper ends of the acid adding cups are communicated with an acid amount control assembly, the lower ends of the acid adding cups are connected with acid adding hoses, and the lower end of the acid adding assembly is provided with an acid adding nozzle assembly capable of longitudinally moving; the acid adding nozzle assembly is communicated with the acid adding hose, and the acid adding assembly is provided with an acid discharging opening and closing mechanism capable of opening and closing the acid adding hose; the vacuum pipeline is externally connected with a vacuum pump, the vacuum pipeline is connected with a vacuum hose, the vacuum hose is communicated with the upper end of the acid adding cup, and the vacuumizing assembly is provided with a vacuum opening and closing mechanism capable of opening and closing the vacuum hose. The vacuum-pumping assembly has the beneficial effects that the acid adding assembly is integrated in the vacuum-pumping assembly, so that the vacuum-pumping operation is carried out before acid adding, the vacuum-pumping and the acid adding share the same pipeline, the pipeline arrangement is convenient, meanwhile, the vacuum-pumping and acid adding effects can be ensured, and the production and processing efficiency and effect are improved.

The colorimetric indicator 5 includes a UV radiation-driven acid releasing agent, a pH indicator that changes color between the deprotonated form and the acid form, a proton scavenger, and a UV stabilizer.

The invention discloses a water-absorbing non-woven fabric and a manufacturing process thereof, and belongs to the technical field of non-woven fabrics, the water-absorbing non-woven fabric is formed by compounding a fiber base material and a functional water-absorbing filler, and the water-absorbing filler mainly comprises alginate, an acid releasing agent and modified zinc oxide with a core-shell structure; wherein the modified zinc oxide is of a microcapsule structure taking nano zinc oxide as a core and a tannic acid-metal coordination compound as a shell; through a core-shell shielding technology, premature reaction among the components is blocked in the storage period, and the storage stability of the material is remarkably improved; when liquid absorption is used, the shell layer disintegrates to release zinc ions and tannic acid, alginate is induced to be subjected to in-situ crosslinking, and a flexible toughening network is constructed. The obtained non-woven fabric not only has high liquid absorption rate and excellent wet strength, can effectively prevent liquid reverse osmosis, but also has remarkable synergistic antibacterial and antioxidant functions, and can greatly prolong the refreshing time of fresh food.

The application belongs to the technical field of DNA extraction, and particularly relates to a field rapid extraction method of DNA in plant bacterial disease lesion, which comprises the following steps: mixing plant disease leaf lesion tissue and a lysis solution, and heating in a water bath in a thermos cup to complete nucleic acid release; the lysis solution comprises 0.05-0.2 mol / L NaOH; preferably, the lysis solution comprises 0.05-0.2 mol / L NaOH, 0.01% SDS and 0.1 mmol / L EDTA. The DNA extraction method established by the application does not need precise instruments and toxic reagents, is simple to operate, short in time consumption, and can realize field rapid extraction of plant lesion bacterial DNA, thereby providing technical support for early diagnosis and accurate prevention and control of plant bacterial diseases in the field.

The application discloses a kind of polyamide acid release sampling waste liquid recovery method.Method: the viscosity of the waste liquid collected together is adjusted by dianhydride solution, so that it is consistent with the viscosity of spinning dope, then the adjusted waste liquid is sent to spinning;The mass concentration and mass of the added dianhydride solution are calculated by the following formula: the required added dianhydride weight T=m*K* (n1*(C-c1)+n2(C-c2)+n3(C-c3)+n4(C-c4)+n5(C-c5)) / (1+C), the added solvent amount L=T*(1+C)*(1-K) / (C*K), the required configuration dianhydride solution mass concentration S=T / (T+L);The application accurately adjusts the viscosity of the collected waste liquid, so that it can continue to spin to realize the reutilization of release sampling waste liquid, can be used for industrialized continuous production to recover release sampling waste liquid in different stages, reduce the discharge of solid waste.

The present invention provides an apparatus and method for grooving after printing a mask in a single-sided electroplating process, including: a water vapor chamber, a first drying chamber, an acidic gas chamber, a first pure water cleaning tank, an alkali cleaning tank, a second pure water cleaning tank, and a second drying chamber that are sequentially arranged for processing silicon wafers; conveying rollers are arranged in the water vapor chamber, the first drying chamber, the acidic gas chamber, the first pure water cleaning tank, the alkali cleaning tank, the second pure water cleaning tank, and the second drying chamber, and the conveying rollers are configured to place and transport silicon wafers. The beneficial effect of the present invention is that the passivation film in the area not covered by the mask is removed by gaseous acid mist, thereby greatly reducing the requirement of the mask material for acid resistance; the HF gas will dissolve into the part of the silicon wafer where water is present to form HF, and HF corrodes the passivation film. The corrosion rate can be controlled by controlling the dissolution amount of HF, and the amount of acid released can be relatively accurately controlled, reducing the consumption of acid.

The invention discloses a pH response type multifunctional slow-release soil conditioner based on raffinate acid and phosphate tailings as well as a preparation method and application of the pH response type multifunctional slow-release soil conditioner, and belongs to the technical field of soil remediation. The method comprises the following steps: carrying out gradient leaching on phosphate tailings by utilizing raffinate acid, and adding a heavy metal curing agent to synchronously realize extraction of effective phosphorus, calcium and magnesium and in-situ curing of heavy metals, so as to obtain eutrophic leachate and detoxified tailing slag; the detoxified tailings are subjected to nanocrystallization and mixed with the leachate, sodium alginate, polyglutamic acid and optional functional additives, granulation and drying are performed through an ionic cross-linking reaction, and the conditioner is prepared. The synergistic high-value utilization of the raffinate acid and the phosphate tailings is realized, and the prepared conditioner has the pH intelligent response slow-release characteristic, can act on the acid soil in a targeting manner, regulates the acid for a long time and releases nutrients. The product has multiple functions of improving the soil structure, supplementing nutrients, passivating heavy metals and the like, realizes treatment of wastes with wastes and change of wastes into valuables, and has a wide application prospect in the fields of environmental protection and agriculture.

This invention relates to the fields of inorganic chemical engineering and textile auxiliaries, and discloses a surface modification process for textile-grade sodium metaantimonate to improve dispersibility. The process includes: adding sodium aluminate to a sodium metaantimonate suspension and stirring until dissolved to create an alkaline liquid environment in which tetrahydroxyaluminate ions are stably present; adding an aqueous solution of phenylphosphonic acid dropwise to this alkaline system for acid-base neutralization, controlling the hydrolysis of aluminum species using the acid release rate, and inducing in-situ generated aluminum hydroxyl species to coordinate with phenylphosphonate ions. This invention constructs an antimony-oxygen-aluminum-oxygen-phosphorus chemical hybrid coating layer without a phase interface on the surface of sodium metaantimonate through a specific acid-base coupling synchronous mineralization mechanism. This kinetically blocks the self-condensation aging path of aluminum species, improves the interfacial bonding force and thermal stability of the modified layer under high temperature and high shear conditions, and effectively solves the problems of thermal desorption and secondary agglomeration of sodium metaantimonate during melt spinning.

The utility model provides a microbe nucleic acid detection micro-fluidic chip and an automatic detection box, a first solenoid valve controls the connection and disconnection of an eluent inlet and an eluent outlet, the eluent flows out of the outlet, passes through a sample chamber of a sample introduction module, and enters a degassing flow channel through the sample inlet; the second electromagnetic valve controls connection and disconnection of an outlet of the degassing flow channel and an inlet of the lysis tank, and nucleic acid release of the sample is completed in the lysis tank; the third electromagnetic valve is used for controlling connection and disconnection between an outlet of the cracking tank and an inlet of the reaction detection tank, the reaction detection tank is provided with a plurality of reaction chambers, freeze-dried powder of an LAMP-CRISPR reaction system is preset in the reaction chambers, and nucleic acid isothermal amplification is completed in the reaction chambers. According to the utility model, the chip card box is fully integrated and has low requirements on operators; a swab is adopted for direct sample loading, and a manual extraction process is not needed; and the chip card box is convenient to install and replace due to the plugging design.

The application relates to the field of textiles, and particularly discloses a water-saving dyeing and finishing process for fluorescent red brocade double-faced cloth, which comprises the following steps: S1, oil removal pretreatment: adding the brocade double-faced cloth base cloth into a treatment liquid added with an oil removal agent, heating, keeping warm, cooling, overflowing, draining water, washing the cloth after water feeding, and draining water to obtain the oil removal base cloth; S2, immersing the oil removal base cloth into a dyeing liquid containing fluorescent red dye and a leveling agent, adding an acid releasing agent after operation treatment, heating, keeping warm, cooling, continuously adding the acid releasing agent, then heating, keeping warm, cooling, draining water, then adding glacial acetic acid and warm water to wash the cloth, draining water, washing the cloth, and preparing primary dyed cloth; and S3, treating the primary dyed cloth in a fixing liquid added with a fluorescent fixing agent, heating, keeping warm, then draining water, washing the cloth, feeding water, and taking out the cloth. The application has the characteristics of improving the fixing effect and providing the water-saving dyeing and finishing process for reducing the production cost.

A package comprising: a sterile barrier packaging film; a self-sterilizing component comprising: a plurality of chlorite ions and water, wherein the self-sterilizing components are substantially free of energy-activated catalysts and acid-releasing compounds; a package interior formed by hermetically sealing the sterile barrier packaging film; and a headspace within the package interior comprising carbon dioxide present in an amount greater than or equal to 5% by volume of the headspace. When the package is exposed to ultraviolet (UV) light having a wavelength of 254 nm, the chlorite ions react with water to produce chlorine dioxide (ClO2), which is released into the headspace. In the absence of any UV light, chlorine dioxide (ClO2) is not produced.