Preparation method of RNA nucleic acid releasing agent and PCR amplification reagent freeze-dried microspheres and application

Abstract

The invention discloses a preparation method of RNA nucleic acid releasing agent and PCR amplification reagent freeze-dried microspheres and application. The invention relates to the technical field of biology, and solves the problems that an RNA nucleic acid releasing agent and a PCR amplification reagent cannot be transported and stored at normal temperature, and the operation is tedious duringuse. The preparation method comprises the following steps: respectively adding the RNA nucleic acid releasing agent and the PCR amplification reagent into a ribozyme-free centrifugal tube in proportion, uniformly mixing to obtain a mixed solution, pouring liquid nitrogen into a sterile medicine cup, dropping the mixed solution into the liquid nitrogen in a volume of 10mu L by using a pipette, condensing into round balls, and using a sterile film to seal the medicine cup, puncturing a plurality of holes in the sterile film, placing the medicine cup in a pre-cooled freeze dryer, setting the coldtrap temperature of the freeze dryer to be -69 DEG C, performing vacuum freeze drying for 6 h or above, and obtaining the RNA nucleic acid releasing agent freeze-dried microsphere and the PCR amplification reagent freeze-dried microsphere respectively. The freeze-dried microspheres are good in stability, convenient to use, the time can be saved, the efficiency is improved, and the cost is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing freeze-dried microspheres of RNA nucleic acid releasing agent and PCR amplification reagent and application thereof. Background technique [0002] Due to its high sensitivity, strong specificity, easy operation, and short time required, nucleic acid detection has been widely used in many fields such as clinical medicine, inspection and quarantine, especially in polymerase chain reaction (English: Polymerase Chain Reaction, referred to as: PCR), isothermal amplification and other nucleic acid amplification techniques. However, nucleic acid amplification technology is more sensitive to interfering substances in clinical or directly obtained specimens, which may cause false negative test results. Therefore, sample nucleic acid extraction and purification are required before nucleic acid amplification. However, the extraction and purification of nucleic acids in s...

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Problems solved by technology

[0005] The present invention provides a method for preparing freeze-dried microspheres of nucleic acid releasing agent and PCR amplification reagent and its application, so as to solve the problem that RNA nucleic acid releasing agent and PCR amplification reagent cannot be transported and stored at room temperature, and the operation is cumbersome during use

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