17 results about "Cytoprotective Agent" patented technology

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention provides an organ perfusate and a preparation method thereof. The Na ion concentration of the organ perfusate is 110-130 mmol / L, and the K ion concentration of the organ perfusate is 20-30 mmol / L; the organ perfusate is prepared from 0.024 mmol / L to 0.036 mmol / L of a colloid osmotic pressure substance, 100 mmol / L to 103 mmol / L of an impermeable substance, 0.001 mmol / L to 0.025 mmol / L of a cell protecting agent, 23 mmol / L to 38 mmol / L of a buffering substance, 3.015 mmol / L to 8.06 mmol / L of calcium and magnesium inorganic salt, 2.5 mmol / L to 6.5 mmol / L of an antioxidant and the balance of an energy substrate. Wherein the molar osmotic pressure of the organ perfusate is 290-320 mOsm / kg, and the viscosity of the organ perfusate at a preset temperature is lower than 2.0 cP; the colloid osmotic pressure substance is Pluronic F-68; and the cell protective agent is thyroxine.

The invention relates to the technical field of biology, and particularly discloses a supercooled cell preserving fluid as well as a preserving method and application thereof. The preserving fluid is mainly prepared from RPX basic cell preserving fluid, an energy substrate, a calcium ion channel blocker, a Na < + > / H < + > exchange channel inhibitor, a colloid impermeable cell protective agent, a cell apoptosis inhibitor, an antioxidant, an inflammation inhibitor, a colloid osmotic pressure regulator, a PH regulator and the like according to a certain proportion. According to the preservation method, the cells are preserved under the subcooling condition of-1 DEG C to-7 DEG C, the cell metabolism rate is reduced to the maximum extent, meanwhile, structural and functional damage caused by freezing to the cells is eliminated, and the effect that the survival rate of the recovered cells exceeds 80% after the cells are preserved for more than 18 months under the non-freezing condition is achieved; the application opens up a way for long-term non-freezing preservation of special functional cells of cells and genetic engineering and clinical cell treatment products, provides an effective method, and lays a foundation for starting a new stage of non-freezing long-term preservation of the cells.

The present invention aims to provide a cytoprotective agent against ferroptosis that contains DHMBA as an active ingredient, by verifying the effect of DHMBA in suppressing ferroptotic cell death using DN model mice and human renal tubular cells HK-2, for each useful agent containing DHMBA as an active ingredient. [Solution] The present invention provides a cell agent for ferroptosis that has a cytoprotective effect against ferroptosis, comprising 3,5-dihydroxy-4-methoxybenzyl alcohol as an active ingredient.

The invention belongs to the technical field of biological medicine, and provides an iron-based nanodot capable of guiding MRI and delivering medicine and a preparation method and application of the iron-based nanodot. The iron-based nanodot is used as a chemotherapeutic drug carrier, and is prepared from a cell protective agent amifostine (AT) and key nutritional metal iron ions (Fe < 3 + >) for inducing ferroptosis through a supramolecular self-assembly technology. The iron-based nanodot can efficiently load the chemotherapeutic drug, improves pharmacokinetic behaviors such as short in-vivo circulation period and fast metabolism of the chemotherapeutic drug, accurately delivers the chemotherapeutic drug to a tumor site, and improves the drug effect of the chemotherapeutic drug; in addition, the iron-based nanodots can realize efficient ferroptosis treatment under the guidance of MRI to induce immunogenic cell death and inhibit growth, metastasis and diffusion of tumor cells.

This invention provides a cell culture additive composition for preventing antibody disulfide bond reduction and its application, relating to the biopharmaceutical field. It comprises the following raw materials: a redox regulator, a metal ion chelator, an antioxidant, an adjuvant, and a cell protectant. This invention inhibits antibody disulfide bond reduction from multiple aspects through the synergistic effect of the redox regulator, metal ion chelator, antioxidant, and buffer. Specifically, the redox regulator maintains a suitable redox potential, the metal ion chelator removes harmful metal ions, the antioxidant removes free radicals, and the buffer maintains pH stability. The components work together synergistically to enhance the effect.

The application discloses an electroporation transfection protection fluid suitable for abalone embryo cell gene editing and application thereof, and belongs to the technical field of gene editing. The electroporation transfection protection fluid comprises a basic fluid and a cell protection agent; the basic fluid comprises NaCl, MgSO4, CaCl2, KCl, NaHCO3, NaBr, K2HPO4, glucose and glutathione; and the cell protection agent comprises polyethylene glycol 400. The electroporation transfection protection fluid can significantly reduce the deformity rate and mortality rate of abalone cells after electroporation technology, improve the gene transfection and gene editing efficiency, and reduce the cost of gene editing experiments, thereby laying a foundation for accelerating marine biological research and promoting environmental protection, species conservation and aquaculture.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.

The invention relates to the technical field of biology, and discloses a recovery liquid for improving recovery efficiency and vitality of organoids, which comprises a metal ion chelating agent, a dissociation agent and a cell protective agent. According to the recovery liquid, matrigel is mildly disintegrated through cooperation of the metal ion chelating agent and the dissociation agent, the organoid can be separated from the matrix within 30 minutes, and the recovery rate of the organoid reaches 90% or above and is far higher than that of a pure mechanical blowing method; the dissociation agent is used for replacing pancreatin and other strong digestive enzymes, and is matched with the cell protective agent, so that the survival rate of the recovered organoid is greater than or equal to 95%, the three-dimensional structure and the intercellular connection are kept complete, and the biological function is real; all the components are clear, the batch is stable, the operation is simple and convenient, the storage is convenient, the culture medium is suitable for passage, collection, downstream cell counting, molecular detection, immunofluorescence, living cell imaging and the like of various sources of organoids such as gastrointestinal tracts, livers and lungs, and the standardization and repeatability of organoid culture are remarkably improved.

[Problem] The purpose of the present invention is to provide, as each useful agent that contains DHMBA as an active ingredient, a cytoprotective agent against ferroptosis, the cytoprotective agent containing DHMBA as an active ingredient and having a cytoprotective action against ferroptosis by verifying the effect of suppressing ferroptosis cell death of DHMBA using a DN model mouse and a human renal tubular cell HK-2. [Solution] The present invention provides a cell agent against ferroptosis, the cell agent containing 3,5-dihydroxy-4-methoxybenzyl alcohol as an active ingredient and having a cytoprotective action against ferroptosis.

The invention provides a lipid composition, a culture medium and a method for multidirectionally differentiating human induced pluripotent stem cells to three germ layers and application of the lipid composition. The culture medium comprises a basic culture medium as well as a lipid combination, a cell protective agent, an antioxidant, a cell nutrition supplement, a non-essential amino acid supplement, an insulin-transferrin-selenium-ethanolamine supplement, a reducing agent, an anti-apoptosis agent and the like which are added into the basic culture medium, the method sequentially comprises the steps of embryoid body formation, multidirectional differentiation and re-paving and adherent culture. According to the lipid composition, the culture medium and the method, the embryoid body with regular form and high yield can be efficiently and stably formed, so that the differentiation of the induced pluripotent stem cells to the trigerm cells is promoted, and the expression level of the trigerm characteristic markers is remarkably improved. The method shows good repeatability and applicability in different induced pluripotent stem cell lines, and provides a reliable tool for standardized evaluation and downstream application of stem cell multidirectional differentiation potential.

The present application relates to a kind of mesenchymal stem cell digestive juice and its preparation method and application, belong to stem cell technical field.The mesenchymal stem cell of the present application includes trypsin, cell protective agent and phosphate buffer solution, in the digestive juice, the concentration of trypsin is 0.02-0.07wt%, the concentration of cell protective agent is 2.01005-7.05005wt%;The cell protective agent is the mixed solution of vitamin C, epidermal growth factor and serum albumin solution.The mesenchymal stem cell digestive juice of the present application, low concentration trypsin is combined with cell protective agent, can be fully dissociated outside mesenchymal stem cell while ensuring that cell is not over-digested by trypsin, and need not to add other reagent to terminate digestion, avoid other animal source serum to contaminate mesenchymal stem cell, reduce the culture cost of mesenchymal stem cell, simplify the culture step of mesenchymal stem cell.

The invention provides a preparation method and application of a mesenchymal stem cell preparation. The preparation method of the mesenchymal stem cell preparation comprises the following steps: S1, taking and separating mesenchymal stem cells; s2, pretreatment of the mesenchymal stem cells; s3, purification and amplification of the mesenchymal stem cells; s4, carrying out induced differentiation on the mesenchymal stem cells to pancreas islet beta-like cells; and S5, preparation preparation. According to the preparation method of the mesenchymal stem cell preparation, the placenta is used as a mesenchymal stem cell source; carrying out pretreatment on the mesenchymal stem cells; sorting the mesenchymal stem cells through immunomagnetic beads; adding a plurality of growth factors and an inducer into an induced differentiation culture medium; and adopting a compound cell protective agent. According to the preparation method disclosed by the invention, the mesenchymal stem cell preparation which is high in purity, strong in activity, good in stability and capable of effectively treating diabetes mellitus can be obtained.

The present invention relates to a method for increasing the activity of NK cells after cryopreservation and recovery and its application. The method comprises: mixing and culturing the NK cells to be frozen with L-proline and β-mercaptoethanol before freezing, and then mixing with NK cell freezing solution to obtain a cell suspension, placing the cell suspension in a sterile freezing tube, and freezing in liquid nitrogen. The present invention creatively finds that adding L-proline and β-mercaptoethanol to the culture system during the NK cell culture stage before freezing can maintain a high cell survival rate and killing ability after freezing and recovery. This method provides an NK cell preservation strategy other than the improvement of the cryopreservation agent formula, which can reduce the amount of cell protectants such as dimethyl sulfoxide in the cryopreservation agent, greatly reduce the toxicity of the cryopreservation solution to the frozen cells and the human body, and reduce costs.

The invention provides a preparation method and application of a CAR-T cell composition targeting a tumor antigen, the composition comprises CAR-T cells, an immunomodulatory factor, a cell protective agent and an effect-prolonging carrier, the mass ratio of the immunomodulatory factor to the CAR-T cells is 0.1-1: 100, the volume ratio of the cell protective agent is 5-15%, and the effect-prolonging carrier is 20-50%. The preparation method comprises the following steps: resuscitating and washing the CAR-T cells, dissolving the immunoregulatory factors, ultrasonically treating the cell protective agent, filtering, mixing according to a specific sequence, and standing. Experiments show that the composition can significantly improve the survival rate of CAR-T cells, enhance the killing activity on tumor cells, reduce the tumor volume and prolong the lifetime in an animal model, and is suitable for preparation of tumor treatment drugs.

The invention provides a separation and purification method, a purity identification method and a component identification and data set construction method of cryptocaryon irritans cyst walls, and relates to the technical field of cryptocaryon irritans detection. The separation and purification method of the cryptocaryon irritans cyst wall comprises the following steps: treating a cyst by using low-concentration sodium hypochlorite and a cell protective agent (DMSO and sterile seawater) containing 1-3wt.% of PMSF, and separating and purifying the cyst wall by using a hypotonic buffer solution and a differential centrifugation and density gradient centrifugation method; a purified capsule wall with a clean surface and complete structure and components is obtained, and the problem that an existing capsule wall is not pure and complete in separation is solved. According to the method for identifying the purity of the capsule wall, a form and molecule two-dimensional verification standard is provided, and the blank of lack of systematic purity identification and control in the prior art is filled. The capsule wall protein data set constructed in the component identification and data set construction method can be used for quickly retrieving the capsule wall components, and an intelligent platform is provided for subsequent diagnosis and drug target screening.