Excrement preserving fluid and preparation method thereof

Abstract

The invention aims to provide an excrement preserving fluid which is applied to excrement preservation. The excrement preserving fluid can preserve an excrement sample for a long time at normal temperature, can effectively inhibit DNA degradation and cell damage in the excrement sample, maintains cell morphology, and has a good inhibiting effect on the activity of degradation enzymes and microbes.The invention also provides a preparation method of the excrement preserving fluid. The excrement preserving fluid prepared by the preparation method is used for preserving excrement. The excrement preserving fluid comprises 10-500 mmol / L of a chelating agent, 1-100 g / L of a bacteriostatic agent, 1-10 g / L of an ionic strength maintaining agent, 1-50% (v / v) of a cell protective agent and 1-50 g / Lof a denaturing agent.

Description

technical field [0001] The invention relates to the technical field of biological preparations, and relates to a feces preservation solution and a preparation method thereof. Background technique [0002] In recent years, the detection of exfoliated cells in human feces is often used in the primary screening of colorectal cancer and other diseases due to its non-invasive, painless and high specificity characteristics. It can better achieve the goal of "early detection, early diagnosis, and early treatment", and from the perspective of tertiary prevention of tumors, emphasizing primary prevention and secondary prevention is the most effective and cost-effective way to reduce the incidence and death of colorectal cancer rate method. [0003] However, the composition of feces is very complex, containing a large amount of digestive residues and various nucleic acid degrading enzymes produced by bacteria such as proteases, DNases (DNases) and RNases (RNases), various PCR inhibit...

AI Technical Summary

Problems solved by technology

However, the collection of stool samples is often not carried out in the laboratory, that is to say, the collected stool samples more or less need to be placed at room temperature for a period of time before they can be stored in the refrigerator at -20°C; A small amount of exfoliated cells is further lost, which affects subsequent detection

[0004] The current method of preservation of feces samples has a short storage time, a low pass rate of sample re-use, harsh storage conditions, and high costs.

Therefore, the current stool sample liquid and preservation methods still need to be improved.