Excrement preserving fluid and preparation method thereof

A technology for preserving liquid and feces, which is applied in the field of feces preservation liquid and its preparation, and can solve the problems of affecting detection, short storage time, loss of exfoliated cells, etc.

Inactive Publication Date: 2020-05-22
广州维帝医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of stool samples is often not carried out in the laboratory, that is to say, the collected stool samples more or less need to be placed at room temperature for a period of time before they can be stored in the refrigerator at -20°C; A small amount of exfoliated cells is ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The stool preservation solution includes the following components:

[0078]The chelating agent is tripotassium ethylenediaminetetraacetic acid, the concentration is 60mmol / L; the antibacterial agent is ethanol, the concentration is 230mL / L; the ionic strength maintenance agent is sodium chloride, the concentration is 9g / L; sulfoxide, the concentration is 30% (v / v); the denaturant is sodium lauryl sulfate, the concentration is 10g / L, the buffer solution is Tris-HCl, and the pH of the feces preservation solution is 7.5.

[0079] The preparation method of feces preservation solution comprises the following steps:

[0080] (1) the tripotassium edetate of 60 molar parts, the ethanol of 230 parts by volume, the sodium chloride of 9 parts by weight, the dimethylsulfoxide of 30 parts by volume and the lauryl sulfuric acid of 10 parts by weight Sodium was dissolved in ultrapure water, and the buffer solution Tris-HCl was used to adjust the pH of the feces preservation solution ...

Embodiment 2-7

[0084] The difference between Examples 2-7 and Example 1 is that the concentration of each component in the obtained feces preservation solution is different, as shown in Table 1 for details.

[0085] The concentration of each component in the excrement preservation liquid of table 1 embodiment 2-7.

[0086] Chelating agent mmol / L Antibacterial agent g / L Sodium chloride g / L Denaturant g / L cell protectant Example 2 10 100 1 50 50％ Example 3 500 1 10 1 20％ Example 4 20 90 5 30 30％ Example 5 300 10 10 5 10％ Example 6 100 10 8 20 40％ Example 7 30 20 10 5 1％

Embodiment 8-11

[0088] The difference between Examples 8-11 and Example 1 is that the pH of the obtained feces preservation solution is different, as shown in Table 2 for details.

[0089] The pH of the feces preservation solution of table 2 embodiment 8-11

[0090] Example 8 Example 9 Example 10 Example 11 pH 6.0 11.0 7.0 8.0

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PUM

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Abstract

The invention aims to provide an excrement preserving fluid which is applied to excrement preservation. The excrement preserving fluid can preserve an excrement sample for a long time at normal temperature, can effectively inhibit DNA degradation and cell damage in the excrement sample, maintains cell morphology, and has a good inhibiting effect on the activity of degradation enzymes and microbes.The invention also provides a preparation method of the excrement preserving fluid. The excrement preserving fluid prepared by the preparation method is used for preserving excrement. The excrement preserving fluid comprises 10-500 mmol/L of a chelating agent, 1-100 g/L of a bacteriostatic agent, 1-10 g/L of an ionic strength maintaining agent, 1-50% (v/v) of a cell protective agent and 1-50 g/Lof a denaturing agent.

Description

technical field [0001] The invention relates to the technical field of biological preparations, and relates to a feces preservation solution and a preparation method thereof. Background technique [0002] In recent years, the detection of exfoliated cells in human feces is often used in the primary screening of colorectal cancer and other diseases due to its non-invasive, painless and high specificity characteristics. It can better achieve the goal of "early detection, early diagnosis, and early treatment", and from the perspective of tertiary prevention of tumors, emphasizing primary prevention and secondary prevention is the most effective and cost-effective way to reduce the incidence and death of colorectal cancer rate method. [0003] However, the composition of feces is very complex, containing a large amount of digestive residues and various nucleic acid degrading enzymes produced by bacteria such as proteases, DNases (DNases) and RNases (RNases), various PCR inhibit...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 赖晓冬邓昕卢月儿
Owner 广州维帝医疗技术有限公司
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