125 results about "Human feces" patented technology

The present invention provides compositions that include an extract of human feces, and methods for using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and / or iatrogenic condition of the colon.

The present invention provides freeze-dried compositions that include an extract of human feces and a cryoprotectant, and methods for making and using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and / or iatrogenic condition of the colon.

The invention relates to a method for realizing harmless comprehensive treatment of human excrements through adopting biotechnology, in particular to a comprehensive technology used for the harmless centralized treatment of human excrements in urban areas. The method comprises the following steps that: (1) human excrements, aquatic plants and terrestrial plants are added in a fermenter, and then a composite strain mixed solution of microzyme, bacillus and lactobacillus is fed into the fermenter so as to carry out aerobic fermentation and anaerobic fermentation in turn; (2) the biogas generated during fermentation is fed into a biogas generating set so as to generate electricity; (3) the products after fermentation undergoes solid-liquid separation, and the solid fermentation products are made into solid organic fertilizer through dewatering, drying, preparation and regranulation; (4) the liquid fermentation products is obtained after solid-liquid separation undergo decoloring, filtration and settlement layering, in the liquid, the sublayer liquid in the solution is made into liquid organic fertilizer through coarse filtration, secondary filtration, sterilization and preparation and the supernatant is made into rinsing meeting effluent standard through aeration and settlement filtration. The method can realize clean, environment-friendly and energy-saving ecological production.

The invention relates to a device for rapid decomposition, sterilization, drying and sanitary treatment of solid / liquid feces, aiming to solve the problems of human feces including environmental pollution, disease transmission and body health damage. The device comprises a solid-liquid separator, a semi-solid finishing machine, a secondary solid-liquid separation spiral pump, a urine treatment tank, a microwave sterilization drying chamber, a dry matter storage tank, and a deodorizing aeration tower. The feces can be rapidly sterilized, deodorized and dried after leaving human body, so as to become sterile and odorless dried particles in a short time, which can be used as fertilizer. The inventive device prevents transmission of pathogenic bacteria from source and solves fecal pollution problem. The inventive device has the advantages of low volume, light weight, and low energy consumption; and can be used in any places. Except fixed places, the device is more suitable for being installed and used in personal vehicles such as passenger train, passenger long-distance bus and passenger ship.

The invention discloses a human feces DNA extraction method. The prior art is complicated and time-consuming; and the trace DNAs extracted by the previous sampling method is too little to perform molecular diagnosis. The extraction method uses selective sample collection PSC equipment for feces samples and adopts a low temperature preservation method. The extraction method comprises the following steps: picking a sample by using a sterilized gun head or sterilized blade and directly placing the picked sample on ice; and adding a feces lysis solution, fully cracking and evenly mixing, adding bovine serum albumin, centrifuging to remove the supernatant, adding protease K to perform a digestion reaction at 58 DEG C, then adding a protein precipitation solution to precipitate the protein, centrifuging to obtain the supernatant, adding a silica gel membrane binding solution to fully bind DNAs, centrifuging to obtain precipitates, adding a silica gel membrane washing solution to wash twice, centrifuging, and adding a TE (Tris + EDTA) buffer solution to finally obtain a preservation solution with DNAs. The human feces DNA extraction method has the advantages of high repeatability and stability, simpleness and short time.

The invention discloses a production method for a biological organic fertilizer containing medium trace element chelates of humic acid. The biological organic fertilizer belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, human feces, cow dung, rabbit manure, vinasse, cassava residue, leftovers in production of monosodium glutamate, trench mud, corn straw, soybean straw, vetch straw, a rapeseed cake, a tea seed cake, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium, actinomyces, a silicate bacterium, trichoderma sp., microzyme, a photosynthetic bacterium, calcium humate, zinc humate, magnesium humate, iron humate, copper humate, and manganese humate used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer containing medium trace element chelates of humic acid. The fertilizer has a complete range of nutrient elements and can fully satisfy needs of a crop for a fertilizer during growth.

Disclosed is a model human-source flora litter model construction and method for detecting flora in litter intestinal tract. The construction steps contain: (1) prepare healthy people fecal bacteria suspension; (2) paunch to farrowing sow to fetch litters; (3) breed the litters in 24-hour aero-ultra filter positive-pressure draft isolation room; (4) inoculate. The detecting steps contain: (1) extract the fecal microbe genome DNA; (2) analyze ERIC-PCR finger spectrum and community DNA crossing; (3) planting analyze bacillus in HFA litter intestinal tract flora; (4) planting analyze HFA bacteroide in HFA litter tract flora. This invention successfully plant human donator's bacillus and bacteroide in litter model intestinal tract. The finger spectrums comparing shows that the flora finger spectrum in litter model intestinal tract has homologous height with that of the human donator. This invention can be used to study the cross-correlation between intestinal tract flora and its host physiology and immune specificity, also it can be used to evaluate regulation and metabolism effect of functional foods or drugs to intestinal tract flora.

The invention discloses a human feces leukocyte automatic recognition method based on machine learning and relates to processing technologies based on digital images, in particular to an automatic recognition and detection method for leukocytes in human feces based on the machine learning technology. According to the method, feature extraction is performed on a sample image, extracted features areadopted to train an SVM (support vector machine), and then the trained vector machine is applied to leukocyte recognition. The method has the advantages of being accurate in recognition, high in speed, high in efficiency and small in calculation amount.

The apparatus and method for detecting blood in urine or feces includes a photodetector configured to detect a transient light emitted in a toilet bowl by luminol and an oxidizer catalyzed by iron in the blood. The apparatus may include dispensers for the luminol, the oxidizer and a base. The apparatus may include a microprocessor and a network connection and may perform statistical analyses, store data and alert the patient or a healthcare provider if blood is detected. The photodetector may be configured to detect light emitted in the toilet bowl by a fluorophore present in the water and excited by the transient light from the luminol and oxidizer.

The invention discloses a lactobacillus strain and application thereof. The lactobacillus CGMCC6495 is separated from pickled vegetable wastewater and stored in a common microorganism center of China Committee for Culture Collection of Microorganisms with the collection date of September 3, 2012 and the collection number of CGMCC No.6495. The strain can be applied to biological deodorization of excrement through the conventional activation culture and enlargement fermentation cultivation; the lactobacillus CGMCC 6495 can be used as a single strain for in-situ deodorization of the human excrement, so that the deodorization effect is stable and the temperature influence is small. Under the normal-temperature condition, the lactobacillus strain has a better deodorization effect in both a laboratory environment and a public toilet site environment.

The invention discloses a method for processing human excrement by utilizing black solider flies to prepare an organic fertilizer, which belongs to the technical field of environmental protection. The method comprises the following steps: filtering excrement dregs through a mesh screen with an aperture of 2-10 cm, and then, adding auxiliary materials to the filtered excrement dregs to prepare the mixture into a culturing material, inoculating ova of the black solider flies on the surface of the culturing material, culturing the ova at 25-35 DEG C for 20-30 days, collecting the culturing material, drying the culturing material, and preparing the culturing material into the organic fertilizer, wherein the auxiliary materials are feather powder, meat and bone meal, saw powder and straw powder. The method provided by the invention has strong processing capability, less occupied field and low energy consumption in a processing process; in the processing process of the human excrement dregs, a biological organic fertilizer and the biologic proteins of maggots of the black solider flies are obtained synchronously; the resource utilization of the human excrement dregs is achieved; waste is changed into valuable articles; not only is the environmental sanitation improved, but also the organic fertilizer and the biologic proteins are produced simultaneously; and the method has quite good application foreground.

The present invention relates to the field of detection of colorectal cancer (CRC), Specifically it relates to methods for the early detection, risk screening and monitoring of CRC and / or adenomatous polyps in a human subject based on the quantification of one or more 18S rDNA bacterial sequences in feces. It further relates to the use of said bacterial sequences as biomarkers of colorectal cancer and / or adenomatous polyps; to a kit comprising a reagent and instructions for the quantification of said bacterial sequences; and to nucleic acids for the quantification of said 16S rDNA bacterial sequences.

The invention provides Bacillus velezensis and a screening culture method and application thereof. The classification name of the Bacillus velezensis is Bacillus velezensis B3-4, the strain is preserved in the China Center for Type Culture Collection on May 29 , 2020, and the preservation number is CCTCC NO: M 2020160. The screening culture method comprises the following steps that a sample iscollected and preserved at low temperature; a beef extract peptone culture medium is coated with diluent of the sample through a gradient dilution method, and constant-temperature culture is conducted at 15-50 DEG C; after the culture medium grows out bacterial colonies, the bacterial colonies with different forms are selected for streak culture; and then, a circle of bacterial colonies are selected for repeated streak culture, and microscopic observation is combined till the purified strain is obtained. The Bacillus velezensis can effectively degrade and remove organic matter and COD in human excrement and domestic sewage.

The invention discloses a method of using firedamp and solar energy as the power resource of thermoelectric generating system. Wherein, using solar energy collector to attain the hot water above 100Deg. C; using heat exchange tube to heat the firedamp generator to keep the ferment temperature inside the firedamp generator at 55-60Deg. C, to attain the high-quality firedamp while the firedamp generate rate is 2.5-3.5m3 / m3 each day; the firedamp can be used as the power resource of thermoelectric generator. Said device comprises a solar energy collector, a firedamp reactor, and a steam generator. The invention realizes generating firedamp at high temperature to confirm the energy of ferment pool, while the firedamp yield of people shit is 0.43m3 / kg, the firedamp of raise yield is 0.12-0.35m3 / kg; and the firedamp yield at high temperature as 45-55Deg. C inside the firedamp ferment pool (digest device) is 2.5-3 times of ferment pool at lower temperature (<35Deg. C). The invention can be used at the industry workshop and the country.

The invention provides a nucleic acid extraction lysate for cast-off cells in human feces. The nucleic acid extraction lysate comprises 1-8 mol / L guanidinium, a 1-20 mM metal ion chelating agent and a100 mM Tris-HCl buffer solution with the pH of 7.5-8.0, wherein guanidinium is selected from at least one of guanidine hydrochloride, guanidinium isothiocyanate and guanidine thiocyanate; the metal ion chelating agent is selected from at least one edetic acid and water soluble salt of edetic acid. Preferably, the nucleic acid extraction lysate further comprises a surfactant with the volume of 1-20%, the surfactant is a non-ionic detergent, and the non-ionic detergent is selected from at least one of Tween-20, Tween-80, Brij-35, NP40 and Triton-100. The invention further provides a related preparation method and application. The nucleic acid extraction lysate can quickly and efficiently realize cell lysis, fully releases nucleic acid, meets the consumption demand for follow-up nucleic aciddetection and is suitable for large-scale popularization.

The invention discloses a winter wheat recommended fertilization method and relates to the technical field of crop fertilization. The method includes the steps that after summer crops are harvested, soil is deeply ploughed or deeply scarified, chicken manure, cow dung and human feces are mashed and mixed to be uniform, the mixture is scattered to the earth surface, and the soil is subjected to rotary tillage and soil preparation; winter wheat seeds are dressed with bactericide or / and insecticide; 3-5 days after rotary tillage and soil preparation, the soil is irrigated before sowing is carried out; 2-3 days after irrigation before sowing is carried out, the soil is subjected to sowing, fertilization and rotary tillage integrated operation; on the last ten days of December, the soil is subjected to warming irrigation; 2-3 days after warming irrigation, straw smashed segments are laid on the earth surface; at the elongation stage of winter wheat, the soil is topdressed, and mepiquat chloride, gibberellins and plant cell cytokinin are sprayed. According to the method, the earth temperature is increased through winter irrigation and laying of the straw smashed segments, water and fertilizer are retained, it is avoided that the wheat is subjected to frost bites and frost damage, so that the normal growth period of the wheat is affected, and the yield of the wheat is stably increased.

The purpose of the present invention is to prepare a pellet by using biomass comprising: food waste to be mass-discharged from regular households or restaurants and the like; feces and urine such as human feces and excrement to be produced by domestic animals; waste wood; and the like, and the present invention relates to a pellet preparation system and preparation method using biomass obtained by sorting plastics, such as a plastic bag into which various waste and food waste are to be put, plastic waste, iron scraps and the like.

The invention provides a method for transforming and purifying total DNA sulfite in human feces. The method comprises the following steps: carrying out thermal denaturation at 95 DEG C for 10-20 minutes, then immediately placing in an iced environment for 5-10 minutes to prevent renaturation, then transforming DNA at 75 DEG C for 45-60 minutes, and adopting immunomagnetic beads and a binding solution containing PEG6000 / 8000 for purifying the transformed DNA to obtain methylated DNA with high transformation rate and high quality, thereby being conducive to standardized and fully automated operation of methylation detection, and being used for analyzing the level of methylation of genes in human DNA in subsequent samples of feces.

The invention relates to the biological technical field, and in particular, relates to a kit for detecting calprotectin in human feces and a preparation method thereof. The kit uses a specific microsphere conjugate sealing reagent instead of a traditional sealant, and can accurately, quantitatively, quickly and sensitively detect the calprotectin in the human feces.

The invention relates to a complete plant for eliminating city dung pollution, wherein the city dung, food residue and the like are conveyed to a maggots keeping factory as protein carriers; after being transformed to high protein feeds, fertilizers, fuels and the like by a complete plant major in keeping maggots, the city dung, food residue and the like are eliminated. The complete plant is composed a dry toilet, a dung warehouse, a dung plastic bag breaking machine, a dung storage warehouse, a packaged multi-layer groove belt maggots keeping frame, a mobile dung vehicle, a maggots slag primary separating device, a dirt maggot cleaning groove, a strong maggot pupating device, an adult maggot spawning dish and the like; the mobile dung vehicle and the maggots slag primary separating device are in fit with the packaged multi-layer groove belt maggots keeping frame; a manner that manures are promoted to important resources can avoid the manures remove the pollution source as wastes. If the complete plant based on protein circulation and used for treating city dung after expanding scale by human optimization is popularized to rural areas again, human feces, straws, weeds, leaves and the like can be included in the optimized protein circulation so as to eliminate organic pollution sources, such as manures and the like; and the tasks of the dietary structure changing to meat from vegetables and large-scale planting of bioenergy, etc are solved.

The invention discloses an intercropping planting cultivation method of oil peony and oil paeonia lactiflora. The method comprises the following steps: (1) selecting a land which is deep, thick and loose in earth, rich in organic substances, sufficient in sunshine, good in draining performance, and low in groundwater level and exposed to the sun; applying mature farmyard manure and slowly-released nutrition particles to the land of each mu, and then deeply ploughing; (2) selecting three-year-old disease-free and healthy seedlings of peony and paeonia lactiflora; (3) digging holes in the prepared earth based on the planting spacing of 40-50 cm*20-25 cm; planting a three-year-old oil peony seedling in each hole; digging holes with the depth of 15 to 20 cm for paeonia lactiflora among the row spacing of the oil peony, and planting a three-year-old oil paeonia lactiflora seedling in each hole; heaping with earth; slightly upwards lifting the seedlings to enable the roots to be in close connection with the earth; covering with earth and compressing; pouring little human feces and urine to fix the roots; covering with fine earth which is higher than the ridge surface; (4) loosening the earth and weeding three to four times each year, and timely hoeing after raining.

The invention discloses green and environment-friendly organic fertilizer capable of effectively improving soil components. The green and environment-friendly organic fertilizer is prepared by, by weight, 16.5 parts of human feces, 18-20 parts of chicken manure, 14.5-16 parts of pig manure, 11.5-13 parts of sheep manure, 10-12 parts of wheat straw, 8.5-10 parts of corn straw, 3.5-5 parts of biogas residue, 2-4 parts of alcohol residue, 1-2 parts of humic acid, 1-2 parts of azotobacteria inoculant, 1.5-2 parts of phosphobacteria inoculant, 2-3 parts of silicate bacteria inoculant, 1.5-2 parts of actinomycetes inoculant, 1-2 parts of bone meal, 1-2 parts of bentonite and 2-4 parts of deodorizing agent.

The invention relates to a harmless treatment and resource utilization method for human feces. The method comprises the following steps: 1, mounting a toilet stool which can separately collect feces and urine in a toilet when a human goes to the toilet, wherein the toilet stool comprises a feces through hole and a urine through hole; 2, connecting the feces through hole to a feces storage device, and connecting the urine through hole to a urine storage device; and 3, separately conveying feces and urine in the feces storage device and the urine storage device to a fermenting system and a urine treatment system for treatment and utilization. According to the method provided by the invention, human excreta can be effectively treated separately, so that the harmless treatment and resource utilization rate of human excreta is increased, and pollution is reduced.

A method for the differentiation of inflammatory bowel disease (IBD) from irritable bowel disease (IBS) followed by distinguishing ulcerative colitis and Crohn's disease from other gastrointestinal illnesses. This highly differential method first uses the presence of elevated lactoferrin as a marker of intestinal inflammation to differentiate IBD from IBS. Patients suspected of IBD are then analyzed for fecal anti-Saccharomyces cerevisiae antibodies (ASCA) as an indicator of Crohn's disease and fecal anti-neutrophil cytoplasmic antibodies (ANCA) as an indicator of ulcerative colitis. IBD patients are further monitored for intestinal inflammation using fecal lactoferrin to evaluate the effectiveness of medical therapy and to predict relapse. The apparatus comprises either a qualitative enzyme-linked immunoassay or other immunoassay that utilizes antibodies specific to human immunoglobins for the measurement of total endogenous lactoferrin, ASCA and ANCA in human feces. The method and apparatus can be used by healthcare providers to identify IBD and distinguish ulcerative colitis from Crohn's disease.

The invention relates to a production method and equipment for conversion of human faeces into a fertilizer and the fertilizer produced thereby. The method comprises the following steps: A, extracting biogas in a septic tank by an extraction machine, using solid and liquid faeces in the septic tank as original slurry; B, filtering the original slurry by a filtering device to remove debris to obtain preliminary faece slurry; C, adding a desiccant into the preliminary faece slurry to stir for mixing in a stirrer to obtain half-dry faece slag; D, drying the biogas by a gas water separation device; E, burning the dried biogas to use the heat of biogas combustion to heat and evaporate the half-dry faece slag obtained by the step C in an evaporation device to obtian dry faece slag. The production method and equipment for conversion of the human faeces into the fertilizer has the advantages of saving resources and reducing pollution, and has good economic benefits and social benefits.

The invention provides a method for dung ecological treatment and resource recycle. Microorganism decomposition is adopted, thus dung dregs can be converted into scentless organic fertilizer resource with extremely farm-oriented value, and dung water can be treated to standard discharging water. Over 95% of reduction treatment can be realized, the dung is released in a mode of carbon dioxide and water. Special bacteria can decompose organisms in the dung into scentless organic fertilizer and water, thus the targets of reduction, harmless and reclamation (a matrix of agricultural organic fertilizer and agriculture in greenhouse) of human dung can be achieved.

The invention discloses a bacterium AD05 which is separated from a human intestinal tract and can generate anti-cancer active metabolites and application of the bacterium AD05. Feces of healthy persons is used as a sample and is screened by the aid of first Gauserime solid culture media with potassium dichromate, so that the bacterium which can generate the anti-cancer active metabolites can be ultimately separated from the human intestinal tract and is named as AD05. The bacterium AD05 and the application have the advantages that the bacterium AD05 has the closest genetic relationship with Fimbriimonas ginsengisoli Gsoil 348 as discovered in 16S rRNA (ribosomal ribonucleic acid) comparison, and obvious anti-tumor activity of the bacterium AD05 and the metabolites of the bacterium AD05 is sufficiently proved by experiments; the bacterium is sourced from the human intestinal tract and accordingly has little or zero side effects for human bodies; a novel technical means is provided for tumor therapy, and important effects can be realized for development of biological tumor therapy.