122 results about "Human feces" patented technology

The present invention provides compositions that include an extract of human feces, and methods for using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and/or iatrogenic condition of the colon.

The present invention provides freeze-dried compositions that include an extract of human feces and a cryoprotectant, and methods for making and using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and/or iatrogenic condition of the colon.

The invention relates to a device for rapid decomposition, sterilization, drying and sanitary treatment of solid/liquid feces, aiming to solve the problems of human feces including environmental pollution, disease transmission and body health damage. The device comprises a solid-liquid separator, a semi-solid finishing machine, a secondary solid-liquid separation spiral pump, a urine treatment tank, a microwave sterilization drying chamber, a dry matter storage tank, and a deodorizing aeration tower. The feces can be rapidly sterilized, deodorized and dried after leaving human body, so as to become sterile and odorless dried particles in a short time, which can be used as fertilizer. The inventive device prevents transmission of pathogenic bacteria from source and solves fecal pollution problem. The inventive device has the advantages of low volume, light weight, and low energy consumption; and can be used in any places. Except fixed places, the device is more suitable for being installed and used in personal vehicles such as passenger train, passenger long-distance bus and passenger ship.

The invention discloses a human feces DNA extraction method. The prior art is complicated and time-consuming; and the trace DNAs extracted by the previous sampling method is too little to perform molecular diagnosis. The extraction method uses selective sample collection PSC equipment for feces samples and adopts a low temperature preservation method. The extraction method comprises the following steps: picking a sample by using a sterilized gun head or sterilized blade and directly placing the picked sample on ice; and adding a feces lysis solution, fully cracking and evenly mixing, adding bovine serum albumin, centrifuging to remove the supernatant, adding protease K to perform a digestion reaction at 58 DEG C, then adding a protein precipitation solution to precipitate the protein, centrifuging to obtain the supernatant, adding a silica gel membrane binding solution to fully bind DNAs, centrifuging to obtain precipitates, adding a silica gel membrane washing solution to wash twice, centrifuging, and adding a TE (Tris + EDTA) buffer solution to finally obtain a preservation solution with DNAs. The human feces DNA extraction method has the advantages of high repeatability and stability, simpleness and short time.

The invention discloses a production method for a biological organic fertilizer containing medium trace element chelates of humic acid. The biological organic fertilizer belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, human feces, cow dung, rabbit manure, vinasse, cassava residue, leftovers in production of monosodium glutamate, trench mud, corn straw, soybean straw, vetch straw, a rapeseed cake, a tea seed cake, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium, actinomyces, a silicate bacterium, trichoderma sp., microzyme, a photosynthetic bacterium, calcium humate, zinc humate, magnesium humate, iron humate, copper humate, and manganese humate used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer containing medium trace element chelates of humic acid. The fertilizer has a complete range of nutrient elements and can fully satisfy needs of a crop for a fertilizer during growth.

Disclosed is a model human-source flora litter model construction and method for detecting flora in litter intestinal tract. The construction steps contain: (1) prepare healthy people fecal bacteria suspension; (2) paunch to farrowing sow to fetch litters; (3) breed the litters in 24-hour aero-ultra filter positive-pressure draft isolation room; (4) inoculate. The detecting steps contain: (1) extract the fecal microbe genome DNA; (2) analyze ERIC-PCR finger spectrum and community DNA crossing; (3) planting analyze bacillus in HFA litter intestinal tract flora; (4) planting analyze HFA bacteroide in HFA litter tract flora. This invention successfully plant human donator's bacillus and bacteroide in litter model intestinal tract. The finger spectrums comparing shows that the flora finger spectrum in litter model intestinal tract has homologous height with that of the human donator. This invention can be used to study the cross-correlation between intestinal tract flora and its host physiology and immune specificity, also it can be used to evaluate regulation and metabolism effect of functional foods or drugs to intestinal tract flora.

The invention discloses a human feces leukocyte automatic recognition method based on machine learning and relates to processing technologies based on digital images, in particular to an automatic recognition and detection method for leukocytes in human feces based on the machine learning technology. According to the method, feature extraction is performed on a sample image, extracted features areadopted to train an SVM (support vector machine), and then the trained vector machine is applied to leukocyte recognition. The method has the advantages of being accurate in recognition, high in speed, high in efficiency and small in calculation amount.

The invention discloses a lactobacillus strain and application thereof. The lactobacillus CGMCC6495 is separated from pickled vegetable wastewater and stored in a common microorganism center of China Committee for Culture Collection of Microorganisms with the collection date of September 3, 2012 and the collection number of CGMCC No.6495. The strain can be applied to biological deodorization of excrement through the conventional activation culture and enlargement fermentation cultivation; the lactobacillus CGMCC 6495 can be used as a single strain for in-situ deodorization of the human excrement, so that the deodorization effect is stable and the temperature influence is small. Under the normal-temperature condition, the lactobacillus strain has a better deodorization effect in both a laboratory environment and a public toilet site environment.

The invention discloses a method of using firedamp and solar energy as the power resource of thermoelectric generating system. Wherein, using solar energy collector to attain the hot water above 100Deg. C; using heat exchange tube to heat the firedamp generator to keep the ferment temperature inside the firedamp generator at 55-60Deg. C, to attain the high-quality firedamp while the firedamp generate rate is 2.5-3.5m3/m3 each day; the firedamp can be used as the power resource of thermoelectric generator. Said device comprises a solar energy collector, a firedamp reactor, and a steam generator. The invention realizes generating firedamp at high temperature to confirm the energy of ferment pool, while the firedamp yield of people shit is 0.43m3/kg, the firedamp of raise yield is 0.12-0.35m3/kg; and the firedamp yield at high temperature as 45-55Deg. C inside the firedamp ferment pool (digest device) is 2.5-3 times of ferment pool at lower temperature (<35Deg. C). The invention can be used at the industry workshop and the country.

The invention provides a nucleic acid extraction lysate for cast-off cells in human feces. The nucleic acid extraction lysate comprises 1-8 mol/L guanidinium, a 1-20 mM metal ion chelating agent and a100 mM Tris-HCl buffer solution with the pH of 7.5-8.0, wherein guanidinium is selected from at least one of guanidine hydrochloride, guanidinium isothiocyanate and guanidine thiocyanate; the metal ion chelating agent is selected from at least one edetic acid and water soluble salt of edetic acid. Preferably, the nucleic acid extraction lysate further comprises a surfactant with the volume of 1-20%, the surfactant is a non-ionic detergent, and the non-ionic detergent is selected from at least one of Tween-20, Tween-80, Brij-35, NP40 and Triton-100. The invention further provides a related preparation method and application. The nucleic acid extraction lysate can quickly and efficiently realize cell lysis, fully releases nucleic acid, meets the consumption demand for follow-up nucleic aciddetection and is suitable for large-scale popularization.

The invention discloses a winter wheat recommended fertilization method and relates to the technical field of crop fertilization. The method includes the steps that after summer crops are harvested, soil is deeply ploughed or deeply scarified, chicken manure, cow dung and human feces are mashed and mixed to be uniform, the mixture is scattered to the earth surface, and the soil is subjected to rotary tillage and soil preparation; winter wheat seeds are dressed with bactericide or/and insecticide; 3-5 days after rotary tillage and soil preparation, the soil is irrigated before sowing is carried out; 2-3 days after irrigation before sowing is carried out, the soil is subjected to sowing, fertilization and rotary tillage integrated operation; on the last ten days of December, the soil is subjected to warming irrigation; 2-3 days after warming irrigation, straw smashed segments are laid on the earth surface; at the elongation stage of winter wheat, the soil is topdressed, and mepiquat chloride, gibberellins and plant cell cytokinin are sprayed. According to the method, the earth temperature is increased through winter irrigation and laying of the straw smashed segments, water and fertilizer are retained, it is avoided that the wheat is subjected to frost bites and frost damage, so that the normal growth period of the wheat is affected, and the yield of the wheat is stably increased.

The purpose of the present invention is to prepare a pellet by using biomass comprising: food waste to be mass-discharged from regular households or restaurants and the like; feces and urine such as human feces and excrement to be produced by domestic animals; waste wood; and the like, and the present invention relates to a pellet preparation system and preparation method using biomass obtained by sorting plastics, such as a plastic bag into which various waste and food waste are to be put, plastic waste, iron scraps and the like.

The invention provides a method for transforming and purifying total DNA sulfite in human feces. The method comprises the following steps: carrying out thermal denaturation at 95 DEG C for 10-20 minutes, then immediately placing in an iced environment for 5-10 minutes to prevent renaturation, then transforming DNA at 75 DEG C for 45-60 minutes, and adopting immunomagnetic beads and a binding solution containing PEG6000/8000 for purifying the transformed DNA to obtain methylated DNA with high transformation rate and high quality, thereby being conducive to standardized and fully automated operation of methylation detection, and being used for analyzing the level of methylation of genes in human DNA in subsequent samples of feces.

The invention relates to a complete plant for eliminating city dung pollution, wherein the city dung, food residue and the like are conveyed to a maggots keeping factory as protein carriers; after being transformed to high protein feeds, fertilizers, fuels and the like by a complete plant major in keeping maggots, the city dung, food residue and the like are eliminated. The complete plant is composed a dry toilet, a dung warehouse, a dung plastic bag breaking machine, a dung storage warehouse, a packaged multi-layer groove belt maggots keeping frame, a mobile dung vehicle, a maggots slag primary separating device, a dirt maggot cleaning groove, a strong maggot pupating device, an adult maggot spawning dish and the like; the mobile dung vehicle and the maggots slag primary separating device are in fit with the packaged multi-layer groove belt maggots keeping frame; a manner that manures are promoted to important resources can avoid the manures remove the pollution source as wastes. If the complete plant based on protein circulation and used for treating city dung after expanding scale by human optimization is popularized to rural areas again, human feces, straws, weeds, leaves and the like can be included in the optimized protein circulation so as to eliminate organic pollution sources, such as manures and the like; and the tasks of the dietary structure changing to meat from vegetables and large-scale planting of bioenergy, etc are solved.

A method for the differentiation of inflammatory bowel disease (IBD) from irritable bowel disease (IBS) followed by distinguishing ulcerative colitis and Crohn's disease from other gastrointestinal illnesses. This highly differential method first uses the presence of elevated lactoferrin as a marker of intestinal inflammation to differentiate IBD from IBS. Patients suspected of IBD are then analyzed for fecal anti-Saccharomyces cerevisiae antibodies (ASCA) as an indicator of Crohn's disease and fecal anti-neutrophil cytoplasmic antibodies (ANCA) as an indicator of ulcerative colitis. IBD patients are further monitored for intestinal inflammation using fecal lactoferrin to evaluate the effectiveness of medical therapy and to predict relapse. The apparatus comprises either a qualitative enzyme-linked immunoassay or other immunoassay that utilizes antibodies specific to human immunoglobins for the measurement of total endogenous lactoferrin, ASCA and ANCA in human feces. The method and apparatus can be used by healthcare providers to identify IBD and distinguish ulcerative colitis from Crohn's disease.

The invention relates to a feces distributing device for storing feces and excrements in toilets, which can evenly store excrement such as feces discharged from the human body in a storage bin, especially can remove the stench caused by excrement in the storage bin, and prevent The reproduction of various bacteria and insects can greatly improve the internal environment of the bathroom. The feces distributing device is arranged between the closet and the storage bin, and can diffuse the feces and excreta falling from the closet under the action of centrifugal force and store them in the storage bin. It includes a turntable arranged between the closet and the storage bin for receiving feces and excrements falling from the closet; a rotating shaft arranged on the turntable; and a driving device for driving the rotating shaft to rotate. The rotation of the turntable under the action of the driving device can make the feces and excreta falling from the toilet diffuse and fall to a position corresponding to the storage space of the storage bin.

The invention discloses a preparation method of farmyard manure for corn planting. The preparation method includes mixing cow dung with pig manure, chicken manure, human feces and composting and leavening agents, and performing aerobic composting and fermenting to obtain first manure; performing aerobic fermentation on water, wheat straw, oil tea cakes, rapeseed oil cakes, distillers' grains, vegetable leaves, fruit peels, dried locusts and EM (effective microorganism) bacteria to obtain second manure; mixing pond mud with nutrient soil and fine sand, and mixing the mixture with the first manure, the second manure and nano carbon powder to obtain third manure; adding seashells and crab shells into a sulfuric acid solution, performing filtration to remove solids, evaporating filter liquor to dryness, adding natural zeolite for mixing, adding the third manure and clear water, stirring for 30 minutes, and adding live earthworms to obtain the farmyard manure for corn planting. The preparation method has simple process, and by the farmyard manure prepared according to the preparation method, yield of corn can be increased effectively.