89 results about "Bioassay" patented technology

The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.

This utility model relates to the field of experimental chamber technology and discloses a multi-angle observation pest biological measurement experimental chamber device, including a supporting base plate and four supporting bases. The top of the supporting base plate has a mounting groove, and a servo motor is fixedly installed on the inner bottom wall of the mounting groove. A supporting box is fixedly installed at the output end of the servo motor. A first hydraulic rod is fixedly installed on the inner top wall of the supporting box, and a fixing ring is fixedly installed at the bottom end of the first hydraulic rod. A supporting disc is detachably connected to the bottom of the fixing ring, and a fixing disc for storage is inserted into the inner bottom wall of the supporting box. This multi-angle observation pest biological measurement experimental chamber device, through the setting of the servo motor, allows the supporting box to rotate when the servo motor is working, thereby facilitating observation of the internal conditions of the supporting box from multiple angles, thus improving the applicability of the device.

The present invention relates to the field of optical nano-imaging bioassays and discloses a bioassay and optical imaging method based on a one-dimensional nanophotonic platform. The method comprises the following steps: (1) a beam of white light is irradiated along a non-periodic direction on a one-dimensional nanostructure, and the generated scattering and diffraction signals are received using a microscope or mobile phone; (2) defects in the one-dimensional nanostructure disrupt the distribution of the local field, reduce the scattering intensity of the nanostructure, and change the color of the one-dimensional nanostructure; (3) in the visible light region, in addition to a scattering peak, another new diffraction peak can improve the sensitivity of the spectral response, allowing the measurement of size differences of 20 nanometers; (4) by modifying antibodies on the surface of the one-dimensional nanostructure, the detection of a single virus can be achieved, which is beneficial for the early diagnosis of in vitro diseases, especially in underdeveloped areas and areas with harsh biological experimental conditions. The technology is simple to operate, low-cost, and conducive to the rapid and high-throughput detection of nanoscale samples.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

Devices and methods for performing rapid low-cost bioassays, including some embodiments for multiplexed detection of multiple amplified biomolecules for identifying one or more diseases, disorders, or infections, are disclosed herein. Such methods can include isolating a first biomolecule in a first detection chamber, isolating a second biomolecule in a second detection chamber, illuminating the first detection chamber with a first light source, and illuminating the second detection chamber with a second light source, where the illumination induces the production of an emitted light signal.

This invention belongs to the field of microbial pesticides and discloses a highly effective Bacillus subtilis strain for killing spider mites and its applications. The Bacillus subtilis strain described in this invention has the preservation number CCTCC NO: M 20251959. Indoor bioassay data show that Bacillus subtilis NBIF-051 has high toxicity against major agricultural mites such as the two-spotted spider mite, the citrus red spider mite, and the cotton red spider mite. Pot experiments showed that after spraying the fermented broth of NBIF-051 for 7 days, the control efficacy against the two-spotted spider mite was 65.18%. Field trials showed that after spraying the fermented broth of this strain for 15 days, the control efficacy against the citrus red spider mite was 63.45%~76.85%, comparable to commercially available chemical acaricides. Furthermore, the acaricidal activity of the fermented broth of this strain exhibits certain tolerance to high temperatures and ultraviolet radiation, showing broad application prospects in agricultural production.

The utility model discloses a test appliance for pesticide soil pretreatment of termites, which relates to the technical field of termite bioassay and comprises a transparent tube body, a first box body and a second box body which are positioned at two ends of the transparent tube body, and connecting mechanisms which are fixedly connected to the outer walls of the first box body and the second box body respectively, the transparent pipe body comprises an outer-layer hollow pipe and an inner-layer hollow pipe, a filter screen is arranged between the outer-layer hollow pipe and the inner-layer hollow pipe, the connecting mechanism comprises connecting boxes which are fixedly connected to the first box body and the second box body respectively, and inserting rods are arranged in the connecting boxes. The transparent tube body is respectively and quickly connected with the first box body and the second box body, complex alignment or tool assistance is not needed, the assembly efficiency is improved, the insertion rod penetrating type connection can ensure that the device is stably connected during movement or vibration, test interruption caused by looseness or failure of a traditional frosted opening is avoided, and the experiment success rate is improved.

The invention relates to bubble deflection within a microfluidic channel. The invention relates to preventing undesirable interference of bubbles with a substance / object of interest in a microfluidic system. In one aspect, the invention provides a microfluidic device (1) for bioassays, comprising: a microfluidic channel, the microfluidic channel is used for guiding a process liquid from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel in a fluid flow direction (F) via at least one region of interest (16) in the microfluidic channel for bioassay; a bubble deflection structure (10) arranged in the microfluidic channel and adapted to deflect bubbles (20) entering the microfluidic channel via the fluid inlet, the bubbles (20) being deflected by the bubble deflection structure (10) when process liquid entering the microfluidic channel via the fluid inlet is allowed to flow through a region of interest (16); such that the bubbles (20) are directed through the microfluidic channel along at least one bubble path (22) formed outside the region of interest (16).

The invention belongs to the technical field of microbial fermentation, and discloses a fermentation medium for improving the live spore number and mosquito killing activity of lysinibacillus sphaericus and application of the fermentation medium. 15 g / L of cottonseed meal; 3 g / L of yeast powder; naCl: 3 g / L; 0.2 g / L of CaCO3; 0.6 g / L of MgSO4. 7H2O; and 0.13 g / L of FeSO4. 7H2O, wherein the initial pH (Potential of Hydrogen) is 8.0. The effect of the optimized culture medium shows that the number of live spores after fermentation of the lysinibacillus sphaericus is in positive correlation with the mosquito killing activity, so that when the formula is screened and optimized, the counting of the live spores is used for replacing bioassay as an evaluation index, and the effects of simplifying the process and shortening the time can be achieved.

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to indomethacin derivatives, their synthesis methods, and applications. The derivatives possess the chemical structures described in formulas (I), (II), and (III). This invention is the first to propose a series of novel indomethacin derivatives. Furthermore, this invention also proposes a synthesis method for indomethacin derivatives, using 5-hydroxy-1-indanone as a base material, to obtain the indomethacin derivatives of this invention through a series of reactions. This synthesis method yields high-yield products that are easily separated, making it the optimal method for preparing the indomethacin derivatives of this invention. Bioassays have confirmed that the indomethacin derivatives provided by this invention exhibit good toxic activity against aphids, spider mites, and armyworms, and can be applied to the control of plant pests.

The utility model relates to a biological assay device for arthropods, and discloses a device for carrying out biological assay on spider mites on living plant leaves, which comprises a base (1), a telescopic device (2) and a storage platform (3). A fixing hole (11) is formed in the base (1); the telescopic device (2) is connected with the base (1) through a bolt, the telescopic device (2) comprises an outer pipe (22) and an inner pipe (21), and the outer pipe (22) is provided with a fixing bolt used for fixing the relative position of the inner pipe (21); the storage platform (3) is fixedly connected with the upper part of the inner pipe (21); the utility model has the advantages that the leaf is not easy to damage, the system stability is good, the position of the culture dish is adjustable, the whole device is easy to move, the observation is easy, and the like, and can better complete the biological assay test of the tetranychid on the living plant leaf.

Described herein are devices and methods for improved detection and quantitation of biomolecules. Devices and methods may include a microfluidic biosensing platform for measuring electrical impedance changes in the presence of target analytes from sinusoidal input voltages to improve the sensitivity of immunoassays. In one embodiment, the described devices and methods may provide a point-of-care immunoassay platform for quantitative diagnostics using signal-enhancing microparticles to improve sensitivity and the limit-of-detection.

The invention discloses a rapid preliminary screening evaluation method for chemical prevention and control active components of meadow moth, which comprises the following steps: (1) evaluating the activity of chemical prevention and control active components to be detected on larvae of meadow moth moth through indoor bioassay to obtain basic score data of the chemical prevention and control active components to be detected; (2) selecting other farmland pests which are damaged with the meadow moth in the same period, and evaluating the activity of the chemical prevention and control active components to be detected through indoor bioassay to obtain additional score data; (3) selecting a main environmental non-target organism, and determining acute toxicity data of the to-be-detected effective component to the environmental non-target organism through public database query or test to obtain score deduction data; (4) comprehensively scoring the chemical prevention and control active components to be detected; and (5) grading according to the comprehensive score, and giving a recommendation conclusion to the chemical prevention and treatment active components to be detected. By means of the evaluation method, the efficient and low-toxicity emergency chemical prevention and control active ingredients of the meadow moth can be rapidly and preliminarily screened, and therefore the purpose of guiding emergency chemical prevention and control of the meadow moth is achieved.

The present invention pertains to methods and systems for automated regulatory-compliant assays. In particular, the present invention pertains, in part, to fully-automated methods and systems for conducting GMP-compliant cell-based bioassays.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.

Provided herein are bioassays to measure the synergistic antibody-dependent enhancement (ADE) effect of SARS-CoV-2 neutralizing antibodies. In particular, the disclosure relates to a method for determining whether two or more SARS-CoV-2 neutralizing antibodies have synergistic ADE effect comprising contacting a combination of said two or more SARS-CoV-2 neutralizing antibodies with FcγR-expressing cells infected with a pseudovirus pseudotyped with SARS-CoV-2 antigen; and determining whether the combination increases the internalization of the pseudovirus into the FcγR-expressing cells synergistically.

The invention discloses fipronil missible oil and a preparation method thereof. Relates to the technical field of pesticide preparations. Comprising the following steps: stirring odorless kerosene and tributyl phosphate; adding nonylphenol polyoxyethylene ether, and stirring; adding dodecylbenzene sulfonic acid calcium salt, and stirring; adding polyoxyethylene nonylphenol phosphate, and stirring; and finally, adding fipronil, and continuously stirring to obtain the fipronil missible oil. The finished product is low in odor, clear, stable and good in water dilution and emulsification; through sand-based bioassay, under the same soil target concentration (g / g), the LC50 of the missible oil for 12 hours and 24 hours is obviously lower than that of the missible oil sold in the market, and the missible oil has a better lethal effect.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

The utility model relates to the technical field of bioassay, and discloses plate scribing equipment and a bioassay system.The plate scribing equipment is characterized by comprising an inoculating loop, a first strain storage area, a plate supporting device, a movable scribing device and a first lifting device; and the inoculating loop is suitable for inoculating strains. The first strain storage area is suitable for placing vessels in which strains are stored; the plate supporting device is suitable for placing a plate. The inoculating loop is connected with the movable lineation device, and the movable lineation device is suitable for driving the inoculating loop to move horizontally so as to drive the inoculating loop to move between the position above the plate supporting device and the position above the first strain storage area and complete the lineation action. The device can replace most manual operation to carry out scribing operation, and does not need to worry that the working efficiency is reduced when the number of measured items is large and tasks are heavy. Compared with complete manual marking operation, the marking operation is faster, more timely and more accurate.

Systems and methods for sorting and imaging insects, including egg and larval life stages, useful for automated high throughput bioassays.

The utility model belongs to the technical field of insect immersion bioassay, and discloses an insect body clamping device for insect immersion bioassay, which comprises a metal cover plate and a hollow square ring, a 60-mesh iron gauze is fixedly arranged on the outer side of the hollow square ring, and handles are fixedly arranged on the right sides of the metal cover plate and the hollow square ring. The two handles rotate mutually through a pin shaft arranged in an inserted mode, an isolation plate is fixedly arranged in the hollow square ring, a 60-mesh iron gauze insect net grid is fixedly arranged in the isolation plate, a pressure spring is fixedly arranged between the two handles, and the clamping device solves the problems that in an insect soaking bioassay experiment, the dosage is large, insect bodies cannot absorb water in time, and an insect soaking cage is inconvenient to clean; the insect immersion bioassay device is simple in structure, low in cost, convenient and fast to use, simple to clean, capable of avoiding cross contamination and reducing experimental errors in insect immersion bioassay, and suitable for popularization and application.

MODULAR LABORATORY APPARATUS FOR PHYTOCHEMICAL EXTRACTION, BIOASSAY AND PHARMACEUTICAL FORMULATION

The invention discloses a holographic three-dimensional bioassay method based on depth label coding and an attention mechanism. The invention relates to a holographic biometric platform employing depth label coding and end-to-end attention-enhanced three-dimensional reconstruction. The platform can enhance the depth of field and volume detection capability, and significantly improve the detection sensitivity and accuracy. A depth label coding strategy is integrated into a neural network architecture based on an attention mechanism, and three-dimensional volume reconstruction is converted into a dual-task framework combining bounding box detection and depth classification. And the limitation of a traditional generation type holographic reconstruction algorithm is overcome, so that accurate real-time three-dimensional positioning is realized. The holographic three-dimensional space positioning is beneficial to expanding field depth application and improving the density of the countable microspheres, chloramphenicol can be detected in a wide dynamic range, and compared with the traditional bioassay based on a two-dimensional hologram, the sensitivity is improved.

This invention discloses a waste glass bottle processing device, including an EBI (Electronic Bioassay) detection device, a crusher, a storage hopper, and a distribution center. The crusher includes a base, a machine body, a feeding hopper, a first drive motor, a transmission mechanism, an active crushing wheel, and a driven crushing wheel. The bottom of the machine body is connected to the top of the base, and the bottom of the feeding hopper is connected to the top of the machine body. The waste glass bottle discharge channel of the EBI detection device is located above the feeding hopper, and a protective baffle is provided circumferentially at the upper end of the feeding hopper. The active and driven crushing wheels are installed in the machine body. The first drive motor drives the active and driven crushing wheels to rotate in opposite directions through the transmission mechanism. The bottom of the storage hopper is equipped with multiple casters and is located inside the base for receiving glass shards and transporting them to the distribution center. This invention can effectively solve the problem of processing and storing waste beer bottles after packaging, effectively improve the on-site management of the packaging line and distribution center, and significantly save labor costs.

The embodiment of the invention provides a solenopsis invicta medicament biological assay device and an application method, and belongs to the technical field of insect prevention and control. The device comprises a bioassay bottle body, a first supporting rod, a hole cover and a bottle cap, a bottle opening is formed in the top face of the bioassay bottle body, the bottle cap is matched with the bottle opening and detachably connected with the bottle opening, the bottom face of the bottle cap is used for smearing a solenopsis invicta bioassay medicament, a through hole is formed in the bottom face of the bioassay bottle body, and the bottom face of the bioassay bottle body is provided with a second supporting rod. The first supporting rod penetrates through the through hole, the top end of the first supporting rod is fixedly connected with the hole cover, the top end of the first supporting rod is used for being supported on the bottle cap through the hole cover, the bottom end of the first supporting rod is used for being inserted into a solenopsis invicta nest, and the hole cover is matched with the through hole. The invention aims to improve the accuracy and efficiency of solenopsis invicta bioassay.

The present invention relates to preparation and use of biocompatible and electrically conductive 3D hydrogels comprising nanocellulose fibrils, such as disintegrated bacterial nanocellulose, plant derived nanocellulose, tunicate derived nanocellulose, or algae derived nanocellulose, together with carbon nanotubes or graphene oxide, as a biocompatible and conductive 3D hydrogel for diagnostics and intervention to mimic or restore tissue and organ function. Biocompatible conductive 3D hydrogels described in this invention can be extruded, casted or injected. The 3D hydrogels described in this invention are cohesive 3D structures and provide electrical conductivity in wet form. 3D hydrogels described in this invention can be further crosslinked using divalent ions such as Calcium ions which improve mechanical stability. Such crosslinking can take place in an animal or human body in a physiological environment after injection into the tissue. 3D hydrogels are biocompatible and show preferable mechanical properties and electrical conductivity through printed lines (4.10−1 S cm−1). The 3D hydrogels prepared by this invention are suited as bioassays to screen drugs against neurodegenerative diseases such as Alzheimer's and Parkinson's, study brain function, and / or be used to link the human brain with electronic and / or communication devices. They can also be injected to replace neural tissue or stimulate guiding of neural cells. They can also be used to inject into the heart and stimulate the heart by using electrical signaling or to repair myocardial infarction.

The present invention relates to cells and antibodies for determining botulinum toxin activity, and methods of using them to measure activity. Currently, in the field of measurement of botulinum toxin potency, there is a need to develop a cell-based potency assay (CBPA) to replace the mouse LD50 bioassay (mLD50). The cells and antibodies of the present invention for botulinum toxin activity assay are cells and antibodies that replace mLD50 with CBPA, and the cell line has a significantly shorter division time and a significantly higher sensitivity to botulinum toxin than the conventional SiMa cells used to determine botulinum toxin activity, and thus is very suitable for detecting toxins and determining cell-based botulinum toxin activity. The CBPA assay using the cells and antibodies of the present invention is expected to become a highly reliable and repeatable cell-based potency assay method for botulinum toxin.

The utility model discloses a portable device for collecting thrips and determining resistance, and belongs to the technical field of insect bioassay. The device comprises an air exhaust gun, a cryopreservation pipe and an insect suction pipe, the insect suction pipe is connected with a fixing block at the bottom of the cryopreservation pipe through a limiting block, a filter screen is arranged at the bottom of the cryopreservation pipe, an annular partition plate is arranged on the upper portion in the cryopreservation pipe, the middle of the annular partition plate is connected with a blocking piece through a torsional spring, and the blocking piece can only be opened towards the interior of the cryopreservation pipe; an opening with the diameter matched with the outer diameter of the insect suction pipe is formed in the middle of the cryopreservation pipe cover, and the insect suction pipe is longer than the cryopreservation pipe and arranged in the cryopreservation pipe; the bottom of the cryopreservation pipe is connected with an air exhaust gun through a rubber sleeve head and an air pipe. The device is easy to assemble and convenient to carry, different medicaments are tested by replacing the cryopreservation tube, the effect of comprehensive and accurate determination is achieved, insect suction and testing are completed in the same device, transfer is not needed, manual errors are reduced, and a more accurate determination result is obtained.

In one aspect, the disclosure relates to a system comprising a plurality of microposts protruding from a membrane opposite a solid substrate, wherein the system can be pneumatically actuated to bring the microposts closer to the solid substrate. Also disclosed herein are methods of constructing the system, methods of performing digital bioassays using the disclosed system, and noninvasive methods for detecting diseases and for monitoring the progress of disease treatments using the disclosed system. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

A microfluidic device for bioassays, including: a microfluidic channel for guiding a process liquid in a direction of fluid flow from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel via at least one region of interest for bioassays within the microfluidic channel; and a bubble deflection structure arranged in the microfluidic channel and adapted to deflect gas bubbles entering the microfluidic channel via the fluid inlet, such that the gas bubbles are guided through the microfluidic channel along at least one bubble path formed outside the region of interest, while allowing process liquid entering the microfluidic channel via the fluid inlet to flow through the region of interest.