127 results about "Bioassay" patented technology

Interconnected computing enables the use of highly diverse environments that support a contemporary civilized operating framework. However, the computational productivity and credibility are disrupted by very immature organizations of this environment. Because current computing entity (e.g., resource) identification infrastructure designs lacking root identification reliability, these environments and their identity infrastructures are decentralized, and unnecessarily unreliable, unsecure, and inadequate of information. This reliability is achieved herein by a bioassay and activity-based portable identification and provenance infrastructure that is substantially accurate and authenticity guaranteed, close to the presence or the quality of the presence. The infrastructure provides ubiquitous available identification information, which can be commonly used in the identification process. Such biometric and activity-based identification information may be acquired contemporaneously and securely fused with or otherwise bound to an associated entity identification information set. This set is used to identify and evaluate entity suitability and / or authenticity, and / or to establish user identities (particular human entities) for individual, social and organizational activities.

The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.

Techniques and systems are disclosed for a bioassay that is an in vitro mimic of peripheral nerve generation using the sensory neurons that innervate the peripheral nervous system. In some embodiments, the techniques may assist in detecting the bioactivity or potency of nerve grafts (e.g., processed, acellular human allografts) for fostering or supporting peripheral nerve regeneration. In various embodiments, techniques comprise affixing a harvested sensory neuron (e.g., a DRG) to a nerve graft segment to form a test construct; culturing the test construct in a medium; analyzing the test construct to indicate the amount of outgrowing peripheral nerve structure; and determining the potency of the nerve graft from a metric derived from the analysis. In some embodiments, techniques and materials may be used to test the effect of a varied test condition on peripheral nerve growth.

This utility model relates to the field of experimental chamber technology and discloses a multi-angle observation pest biological measurement experimental chamber device, including a supporting base plate and four supporting bases. The top of the supporting base plate has a mounting groove, and a servo motor is fixedly installed on the inner bottom wall of the mounting groove. A supporting box is fixedly installed at the output end of the servo motor. A first hydraulic rod is fixedly installed on the inner top wall of the supporting box, and a fixing ring is fixedly installed at the bottom end of the first hydraulic rod. A supporting disc is detachably connected to the bottom of the fixing ring, and a fixing disc for storage is inserted into the inner bottom wall of the supporting box. This multi-angle observation pest biological measurement experimental chamber device, through the setting of the servo motor, allows the supporting box to rotate when the servo motor is working, thereby facilitating observation of the internal conditions of the supporting box from multiple angles, thus improving the applicability of the device.

The present invention relates to the field of optical nano-imaging bioassays and discloses a bioassay and optical imaging method based on a one-dimensional nanophotonic platform. The method comprises the following steps: (1) a beam of white light is irradiated along a non-periodic direction on a one-dimensional nanostructure, and the generated scattering and diffraction signals are received using a microscope or mobile phone; (2) defects in the one-dimensional nanostructure disrupt the distribution of the local field, reduce the scattering intensity of the nanostructure, and change the color of the one-dimensional nanostructure; (3) in the visible light region, in addition to a scattering peak, another new diffraction peak can improve the sensitivity of the spectral response, allowing the measurement of size differences of 20 nanometers; (4) by modifying antibodies on the surface of the one-dimensional nanostructure, the detection of a single virus can be achieved, which is beneficial for the early diagnosis of in vitro diseases, especially in underdeveloped areas and areas with harsh biological experimental conditions. The technology is simple to operate, low-cost, and conducive to the rapid and high-throughput detection of nanoscale samples.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

Provided herein are 3D bioassays to measure antibody-dependent cell-mediated cytotoxicity (ADCC). In particular, provided is a method for assessing ADCC comprising contacting an antibody or a functional antibody fragment thereof with a target cell and an effector cell expressing FcR, in a 3D cell culture under a condition allowing the interactions between those substances; and detecting the changes in the expression levels of biomarkers CXCL9, CXCL10 and CXCL11 and 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 of the biomarkers selected from UBD, IDOL, STEAP4, JAG1, APOL4, GBP4, CD274, GBP5, CCL3 and CCL4; and assessing ADCC of the antibody based on the expression results. Also provided are the use and product relate to the method.

Devices and methods for performing rapid low-cost bioassays, including some embodiments for multiplexed detection of multiple amplified biomolecules for identifying one or more diseases, disorders, or infections, are disclosed herein. Such methods can include isolating a first biomolecule in a first detection chamber, isolating a second biomolecule in a second detection chamber, illuminating the first detection chamber with a first light source, and illuminating the second detection chamber with a second light source, where the illumination induces the production of an emitted light signal.

This invention belongs to the field of microbial pesticides and discloses a highly effective Bacillus subtilis strain for killing spider mites and its applications. The Bacillus subtilis strain described in this invention has the preservation number CCTCC NO: M 20251959. Indoor bioassay data show that Bacillus subtilis NBIF-051 has high toxicity against major agricultural mites such as the two-spotted spider mite, the citrus red spider mite, and the cotton red spider mite. Pot experiments showed that after spraying the fermented broth of NBIF-051 for 7 days, the control efficacy against the two-spotted spider mite was 65.18%. Field trials showed that after spraying the fermented broth of this strain for 15 days, the control efficacy against the citrus red spider mite was 63.45%~76.85%, comparable to commercially available chemical acaricides. Furthermore, the acaricidal activity of the fermented broth of this strain exhibits certain tolerance to high temperatures and ultraviolet radiation, showing broad application prospects in agricultural production.

Systems and methods for performing biological assays using heat-sealed valves and / or incubation chambers are provided herein. The systems and methods determine one or more characteristics of a nucleic acid amplified sample based on modified optical properties of the sample.

Homogeneous human amniotic fluid stem cell clones are provided. The clones serve as direct bioassays to test the effect of drugs, nutraceuticals, vitamins and toxic agents on fetus growth, differentiation and development, and may be administered in form of pharmaceutical compositions for reconstructive engineering and for the treatment of cardiac, neurological and osteoarthritic diseases.

The utility model discloses a test appliance for pesticide soil pretreatment of termites, which relates to the technical field of termite bioassay and comprises a transparent tube body, a first box body and a second box body which are positioned at two ends of the transparent tube body, and connecting mechanisms which are fixedly connected to the outer walls of the first box body and the second box body respectively, the transparent pipe body comprises an outer-layer hollow pipe and an inner-layer hollow pipe, a filter screen is arranged between the outer-layer hollow pipe and the inner-layer hollow pipe, the connecting mechanism comprises connecting boxes which are fixedly connected to the first box body and the second box body respectively, and inserting rods are arranged in the connecting boxes. The transparent tube body is respectively and quickly connected with the first box body and the second box body, complex alignment or tool assistance is not needed, the assembly efficiency is improved, the insertion rod penetrating type connection can ensure that the device is stably connected during movement or vibration, test interruption caused by looseness or failure of a traditional frosted opening is avoided, and the experiment success rate is improved.

The invention relates to bubble deflection within a microfluidic channel. The invention relates to preventing undesirable interference of bubbles with a substance / object of interest in a microfluidic system. In one aspect, the invention provides a microfluidic device (1) for bioassays, comprising: a microfluidic channel, the microfluidic channel is used for guiding a process liquid from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel in a fluid flow direction (F) via at least one region of interest (16) in the microfluidic channel for bioassay; a bubble deflection structure (10) arranged in the microfluidic channel and adapted to deflect bubbles (20) entering the microfluidic channel via the fluid inlet, the bubbles (20) being deflected by the bubble deflection structure (10) when process liquid entering the microfluidic channel via the fluid inlet is allowed to flow through a region of interest (16); such that the bubbles (20) are directed through the microfluidic channel along at least one bubble path (22) formed outside the region of interest (16).

The invention belongs to the technical field of microbial fermentation, and discloses a fermentation medium for improving the live spore number and mosquito killing activity of lysinibacillus sphaericus and application of the fermentation medium. 15 g / L of cottonseed meal; 3 g / L of yeast powder; naCl: 3 g / L; 0.2 g / L of CaCO3; 0.6 g / L of MgSO4. 7H2O; and 0.13 g / L of FeSO4. 7H2O, wherein the initial pH (Potential of Hydrogen) is 8.0. The effect of the optimized culture medium shows that the number of live spores after fermentation of the lysinibacillus sphaericus is in positive correlation with the mosquito killing activity, so that when the formula is screened and optimized, the counting of the live spores is used for replacing bioassay as an evaluation index, and the effects of simplifying the process and shortening the time can be achieved.

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to indomethacin derivatives, their synthesis methods, and applications. The derivatives possess the chemical structures described in formulas (I), (II), and (III). This invention is the first to propose a series of novel indomethacin derivatives. Furthermore, this invention also proposes a synthesis method for indomethacin derivatives, using 5-hydroxy-1-indanone as a base material, to obtain the indomethacin derivatives of this invention through a series of reactions. This synthesis method yields high-yield products that are easily separated, making it the optimal method for preparing the indomethacin derivatives of this invention. Bioassays have confirmed that the indomethacin derivatives provided by this invention exhibit good toxic activity against aphids, spider mites, and armyworms, and can be applied to the control of plant pests.

The utility model relates to a biological assay device for arthropods, and discloses a device for carrying out biological assay on spider mites on living plant leaves, which comprises a base (1), a telescopic device (2) and a storage platform (3). A fixing hole (11) is formed in the base (1); the telescopic device (2) is connected with the base (1) through a bolt, the telescopic device (2) comprises an outer pipe (22) and an inner pipe (21), and the outer pipe (22) is provided with a fixing bolt used for fixing the relative position of the inner pipe (21); the storage platform (3) is fixedly connected with the upper part of the inner pipe (21); the utility model has the advantages that the leaf is not easy to damage, the system stability is good, the position of the culture dish is adjustable, the whole device is easy to move, the observation is easy, and the like, and can better complete the biological assay test of the tetranychid on the living plant leaf.

Described herein are devices and methods for improved detection and quantitation of biomolecules. Devices and methods may include a microfluidic biosensing platform for measuring electrical impedance changes in the presence of target analytes from sinusoidal input voltages to improve the sensitivity of immunoassays. In one embodiment, the described devices and methods may provide a point-of-care immunoassay platform for quantitative diagnostics using signal-enhancing microparticles to improve sensitivity and the limit-of-detection.

The present disclosure is directed to a device (100) comprising a sampling part (110), wherein the sampling part (110) comprises an array of capture zones (112) for sampling liquid volumes between tens of microliters and femtoliters, wherein some or all of the capture zones (112) contain a sponge-like material. Also disclosed are a method for the preparation of such a device and a method for the detection and determination of the presence, concentration and / or properties of an analyte by contacting a liquid sample with such a device.

The invention discloses a rapid preliminary screening evaluation method for chemical prevention and control active components of meadow moth, which comprises the following steps: (1) evaluating the activity of chemical prevention and control active components to be detected on larvae of meadow moth moth through indoor bioassay to obtain basic score data of the chemical prevention and control active components to be detected; (2) selecting other farmland pests which are damaged with the meadow moth in the same period, and evaluating the activity of the chemical prevention and control active components to be detected through indoor bioassay to obtain additional score data; (3) selecting a main environmental non-target organism, and determining acute toxicity data of the to-be-detected effective component to the environmental non-target organism through public database query or test to obtain score deduction data; (4) comprehensively scoring the chemical prevention and control active components to be detected; and (5) grading according to the comprehensive score, and giving a recommendation conclusion to the chemical prevention and treatment active components to be detected. By means of the evaluation method, the efficient and low-toxicity emergency chemical prevention and control active ingredients of the meadow moth can be rapidly and preliminarily screened, and therefore the purpose of guiding emergency chemical prevention and control of the meadow moth is achieved.

The invention belongs to the technical field of bioassay, and discloses a monoclonal antibody purification device and a concentration determination method. The purification device can be used for separating and purifying the monoclonal antibody in the monoclonal antibody cell fermentation liquid supernatant, and comprises an injector, an affinity chromatography medium and an injection pump. The concentration determination method is carried out by using the purification device, and comprises the following steps: carrying out affinity chromatography on the fermentation supernatant of the monoclonal antibody, collecting an eluent containing the target monoclonal antibody, determining the concentration of the target monoclonal antibody in the eluent, and calculating the concentration of the target monoclonal antibody in the fermentation supernatant. The determination method is at least suitable for purification and content determination of fermentation supernatant of existing monoclonal antibodies QX004N, QX005N, QX006N, QX007N, QX008N, QX002N, QX013N and QX001S, and has the advantages that expensive equipment is not needed, the cost is low, the operation is simple, the universality is high, and the result is basically consistent with that of a high performance liquid chromatography.

The present invention pertains to methods and systems for automated regulatory-compliant assays. In particular, the present invention pertains, in part, to fully-automated methods and systems for conducting GMP-compliant cell-based bioassays.

The present application belongs to the field of microbial pesticides, and relates to a bacillus megaterium NBNC-104 for preventing and treating root-knot nematodes, a compound microbial agent and application thereof. The preservation number of the bacillus megaterium strain NBNC-104 is CCTCC M 20242324. Laboratory bioassay data show that the bacillus megaterium NBNC-104 has high toxic killing activity and repellent effect on southern root-knot nematodes, and has synergistic nematode killing activity after being compounded with bacillus thuringiensis strain NBIN-863 and kunming streptomyces sp. NBNH-006, and the strain can effectively colonize in the rhizosphere soil environment within 30 days under potting conditions. Therefore, the strain has broad application prospects in agricultural production.

The present invention relates to microbiology and plant pathology technology, and specifically to a method of using a small centrifuge tube to mix a small amount of drug sample liquid with water agar under the working condition of a microplate mixing station with a temperature system of 65°C to prepare an agar microplate containing drugs. The main components of the microplate mixing station include a temperature control body A, a centrifuge tube preheating component B for mixing samples, a drug sample preheating component C, a gun head preheating component D, and a glass slide preheating iron box component E. The main steps of the preparation method of the drug microplate are as follows: 1) preparing drug samples, 2) preparing water agar for mixing samples, 3) preparing gun heads, 4) starting the microplate mixing station to enter the working state, 5) preheating samples, 6) preheating glass slides, 7) transferring sample liquid, 8) transferring water agar, 9) mixing sample liquid and water agar, and 10) dripping and developing into a drug microplate. The advantages of the present invention are as follows: 1) small centrifuge tubes are commonly used consumables in biological laboratories and are convenient for use. 2) the total amount of drug samples required is very small; 3) the obtained drug microplate is convenient for subsequent biological assays and microscopic observations.

Provided herein are bioassays to measure the synergistic antibody-dependent enhancement (ADE) effect of SARS-CoV-2 neutralizing antibodies. In particular, the disclosure relates to a method for determining whether two or more SARS-CoV-2 neutralizing antibodies have synergistic ADE effect comprising contacting a combination of said two or more SARS-CoV-2 neutralizing antibodies with FcγR-expressing cells infected with a pseudovirus pseudotyped with SARS-CoV-2 antigen; and determining whether the combination increases the internalization of the pseudovirus into the FcγR-expressing cells synergistically.

The invention discloses fipronil missible oil and a preparation method thereof. Relates to the technical field of pesticide preparations. Comprising the following steps: stirring odorless kerosene and tributyl phosphate; adding nonylphenol polyoxyethylene ether, and stirring; adding dodecylbenzene sulfonic acid calcium salt, and stirring; adding polyoxyethylene nonylphenol phosphate, and stirring; and finally, adding fipronil, and continuously stirring to obtain the fipronil missible oil. The finished product is low in odor, clear, stable and good in water dilution and emulsification; through sand-based bioassay, under the same soil target concentration (g / g), the LC50 of the missible oil for 12 hours and 24 hours is obviously lower than that of the missible oil sold in the market, and the missible oil has a better lethal effect.

The invention provides a benzoxazine-Tioxazafen derivative as well as a preparation method and application thereof, and belongs to the technical field of preparation of pesticide compounds. The benzoxazine-Tioxazafen derivative disclosed by the invention is a derivative of 3-(5-(thiophene-2-yl)-1, 2, 4-oxadiazole-3-yl)-2H-benzo [b] [1, 4] oxazine, and the structural formula of the benzoxazine-Tioxazafen derivative is shown in the description. According to the invention, Tioxazafen is used as a parent structure and is subjected to active substructure splicing with different substituted benzoxazine derivatives to synthesize a series of new compounds, the chemical structure is novel, and the compound can be used for preventing and treating plant parasitic nematodes such as root-knot nematodes and pine wood nematodes; a biological assay result shows that the activity of the benzoxazine-Tioxazafen derivative to pine wood nematodes is obviously superior to that of a commercial medicament Tioxazafen, and is equivalent to that of lythidathion, so that the benzoxazine-Tioxazafen derivative has a relatively high commercial prospect.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

The utility model relates to the technical field of bioassay, and discloses plate scribing equipment and a bioassay system.The plate scribing equipment is characterized by comprising an inoculating loop, a first strain storage area, a plate supporting device, a movable scribing device and a first lifting device; and the inoculating loop is suitable for inoculating strains. The first strain storage area is suitable for placing vessels in which strains are stored; the plate supporting device is suitable for placing a plate. The inoculating loop is connected with the movable lineation device, and the movable lineation device is suitable for driving the inoculating loop to move horizontally so as to drive the inoculating loop to move between the position above the plate supporting device and the position above the first strain storage area and complete the lineation action. The device can replace most manual operation to carry out scribing operation, and does not need to worry that the working efficiency is reduced when the number of measured items is large and tasks are heavy. Compared with complete manual marking operation, the marking operation is faster, more timely and more accurate.

Systems and methods for sorting and imaging insects, including egg and larval life stages, useful for automated high throughput bioassays.

The utility model belongs to the technical field of insect immersion bioassay, and discloses an insect body clamping device for insect immersion bioassay, which comprises a metal cover plate and a hollow square ring, a 60-mesh iron gauze is fixedly arranged on the outer side of the hollow square ring, and handles are fixedly arranged on the right sides of the metal cover plate and the hollow square ring. The two handles rotate mutually through a pin shaft arranged in an inserted mode, an isolation plate is fixedly arranged in the hollow square ring, a 60-mesh iron gauze insect net grid is fixedly arranged in the isolation plate, a pressure spring is fixedly arranged between the two handles, and the clamping device solves the problems that in an insect soaking bioassay experiment, the dosage is large, insect bodies cannot absorb water in time, and an insect soaking cage is inconvenient to clean; the insect immersion bioassay device is simple in structure, low in cost, convenient and fast to use, simple to clean, capable of avoiding cross contamination and reducing experimental errors in insect immersion bioassay, and suitable for popularization and application.