48 results about "Bioassay" patented technology

This utility model relates to the field of experimental chamber technology and discloses a multi-angle observation pest biological measurement experimental chamber device, including a supporting base plate and four supporting bases. The top of the supporting base plate has a mounting groove, and a servo motor is fixedly installed on the inner bottom wall of the mounting groove. A supporting box is fixedly installed at the output end of the servo motor. A first hydraulic rod is fixedly installed on the inner top wall of the supporting box, and a fixing ring is fixedly installed at the bottom end of the first hydraulic rod. A supporting disc is detachably connected to the bottom of the fixing ring, and a fixing disc for storage is inserted into the inner bottom wall of the supporting box. This multi-angle observation pest biological measurement experimental chamber device, through the setting of the servo motor, allows the supporting box to rotate when the servo motor is working, thereby facilitating observation of the internal conditions of the supporting box from multiple angles, thus improving the applicability of the device.

Devices and methods for performing rapid low-cost bioassays, including some embodiments for multiplexed detection of multiple amplified biomolecules for identifying one or more diseases, disorders, or infections, are disclosed herein. Such methods can include isolating a first biomolecule in a first detection chamber, isolating a second biomolecule in a second detection chamber, illuminating the first detection chamber with a first light source, and illuminating the second detection chamber with a second light source, where the illumination induces the production of an emitted light signal.

This invention belongs to the field of microbial pesticides and discloses a highly effective Bacillus subtilis strain for killing spider mites and its applications. The Bacillus subtilis strain described in this invention has the preservation number CCTCC NO: M 20251959. Indoor bioassay data show that Bacillus subtilis NBIF-051 has high toxicity against major agricultural mites such as the two-spotted spider mite, the citrus red spider mite, and the cotton red spider mite. Pot experiments showed that after spraying the fermented broth of NBIF-051 for 7 days, the control efficacy against the two-spotted spider mite was 65.18%. Field trials showed that after spraying the fermented broth of this strain for 15 days, the control efficacy against the citrus red spider mite was 63.45%~76.85%, comparable to commercially available chemical acaricides. Furthermore, the acaricidal activity of the fermented broth of this strain exhibits certain tolerance to high temperatures and ultraviolet radiation, showing broad application prospects in agricultural production.

The utility model discloses a test appliance for pesticide soil pretreatment of termites, which relates to the technical field of termite bioassay and comprises a transparent tube body, a first box body and a second box body which are positioned at two ends of the transparent tube body, and connecting mechanisms which are fixedly connected to the outer walls of the first box body and the second box body respectively, the transparent pipe body comprises an outer-layer hollow pipe and an inner-layer hollow pipe, a filter screen is arranged between the outer-layer hollow pipe and the inner-layer hollow pipe, the connecting mechanism comprises connecting boxes which are fixedly connected to the first box body and the second box body respectively, and inserting rods are arranged in the connecting boxes. The transparent tube body is respectively and quickly connected with the first box body and the second box body, complex alignment or tool assistance is not needed, the assembly efficiency is improved, the insertion rod penetrating type connection can ensure that the device is stably connected during movement or vibration, test interruption caused by looseness or failure of a traditional frosted opening is avoided, and the experiment success rate is improved.

The invention relates to bubble deflection within a microfluidic channel. The invention relates to preventing undesirable interference of bubbles with a substance / object of interest in a microfluidic system. In one aspect, the invention provides a microfluidic device (1) for bioassays, comprising: a microfluidic channel, the microfluidic channel is used for guiding a process liquid from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel in a fluid flow direction (F) via at least one region of interest (16) in the microfluidic channel for bioassay; a bubble deflection structure (10) arranged in the microfluidic channel and adapted to deflect bubbles (20) entering the microfluidic channel via the fluid inlet, the bubbles (20) being deflected by the bubble deflection structure (10) when process liquid entering the microfluidic channel via the fluid inlet is allowed to flow through a region of interest (16); such that the bubbles (20) are directed through the microfluidic channel along at least one bubble path (22) formed outside the region of interest (16).

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to indomethacin derivatives, their synthesis methods, and applications. The derivatives possess the chemical structures described in formulas (I), (II), and (III). This invention is the first to propose a series of novel indomethacin derivatives. Furthermore, this invention also proposes a synthesis method for indomethacin derivatives, using 5-hydroxy-1-indanone as a base material, to obtain the indomethacin derivatives of this invention through a series of reactions. This synthesis method yields high-yield products that are easily separated, making it the optimal method for preparing the indomethacin derivatives of this invention. Bioassays have confirmed that the indomethacin derivatives provided by this invention exhibit good toxic activity against aphids, spider mites, and armyworms, and can be applied to the control of plant pests.

The utility model relates to a biological assay device for arthropods, and discloses a device for carrying out biological assay on spider mites on living plant leaves, which comprises a base (1), a telescopic device (2) and a storage platform (3). A fixing hole (11) is formed in the base (1); the telescopic device (2) is connected with the base (1) through a bolt, the telescopic device (2) comprises an outer pipe (22) and an inner pipe (21), and the outer pipe (22) is provided with a fixing bolt used for fixing the relative position of the inner pipe (21); the storage platform (3) is fixedly connected with the upper part of the inner pipe (21); the utility model has the advantages that the leaf is not easy to damage, the system stability is good, the position of the culture dish is adjustable, the whole device is easy to move, the observation is easy, and the like, and can better complete the biological assay test of the tetranychid on the living plant leaf.

Described herein are devices and methods for improved detection and quantitation of biomolecules. Devices and methods may include a microfluidic biosensing platform for measuring electrical impedance changes in the presence of target analytes from sinusoidal input voltages to improve the sensitivity of immunoassays. In one embodiment, the described devices and methods may provide a point-of-care immunoassay platform for quantitative diagnostics using signal-enhancing microparticles to improve sensitivity and the limit-of-detection.

Provided herein are bioassays to measure the synergistic antibody-dependent enhancement (ADE) effect of SARS-CoV-2 neutralizing antibodies. In particular, the disclosure relates to a method for determining whether two or more SARS-CoV-2 neutralizing antibodies have synergistic ADE effect comprising contacting a combination of said two or more SARS-CoV-2 neutralizing antibodies with FcγR-expressing cells infected with a pseudovirus pseudotyped with SARS-CoV-2 antigen; and determining whether the combination increases the internalization of the pseudovirus into the FcγR-expressing cells synergistically.

The invention discloses fipronil missible oil and a preparation method thereof. Relates to the technical field of pesticide preparations. Comprising the following steps: stirring odorless kerosene and tributyl phosphate; adding nonylphenol polyoxyethylene ether, and stirring; adding dodecylbenzene sulfonic acid calcium salt, and stirring; adding polyoxyethylene nonylphenol phosphate, and stirring; and finally, adding fipronil, and continuously stirring to obtain the fipronil missible oil. The finished product is low in odor, clear, stable and good in water dilution and emulsification; through sand-based bioassay, under the same soil target concentration (g / g), the LC50 of the missible oil for 12 hours and 24 hours is obviously lower than that of the missible oil sold in the market, and the missible oil has a better lethal effect.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

Systems and methods for sorting and imaging insects, including egg and larval life stages, useful for automated high throughput bioassays.

The invention discloses a holographic three-dimensional bioassay method based on depth label coding and an attention mechanism. The invention relates to a holographic biometric platform employing depth label coding and end-to-end attention-enhanced three-dimensional reconstruction. The platform can enhance the depth of field and volume detection capability, and significantly improve the detection sensitivity and accuracy. A depth label coding strategy is integrated into a neural network architecture based on an attention mechanism, and three-dimensional volume reconstruction is converted into a dual-task framework combining bounding box detection and depth classification. And the limitation of a traditional generation type holographic reconstruction algorithm is overcome, so that accurate real-time three-dimensional positioning is realized. The holographic three-dimensional space positioning is beneficial to expanding field depth application and improving the density of the countable microspheres, chloramphenicol can be detected in a wide dynamic range, and compared with the traditional bioassay based on a two-dimensional hologram, the sensitivity is improved.

This invention discloses a waste glass bottle processing device, including an EBI (Electronic Bioassay) detection device, a crusher, a storage hopper, and a distribution center. The crusher includes a base, a machine body, a feeding hopper, a first drive motor, a transmission mechanism, an active crushing wheel, and a driven crushing wheel. The bottom of the machine body is connected to the top of the base, and the bottom of the feeding hopper is connected to the top of the machine body. The waste glass bottle discharge channel of the EBI detection device is located above the feeding hopper, and a protective baffle is provided circumferentially at the upper end of the feeding hopper. The active and driven crushing wheels are installed in the machine body. The first drive motor drives the active and driven crushing wheels to rotate in opposite directions through the transmission mechanism. The bottom of the storage hopper is equipped with multiple casters and is located inside the base for receiving glass shards and transporting them to the distribution center. This invention can effectively solve the problem of processing and storing waste beer bottles after packaging, effectively improve the on-site management of the packaging line and distribution center, and significantly save labor costs.

The utility model discloses a portable device for collecting thrips and determining resistance, and belongs to the technical field of insect bioassay. The device comprises an air exhaust gun, a cryopreservation pipe and an insect suction pipe, the insect suction pipe is connected with a fixing block at the bottom of the cryopreservation pipe through a limiting block, a filter screen is arranged at the bottom of the cryopreservation pipe, an annular partition plate is arranged on the upper portion in the cryopreservation pipe, the middle of the annular partition plate is connected with a blocking piece through a torsional spring, and the blocking piece can only be opened towards the interior of the cryopreservation pipe; an opening with the diameter matched with the outer diameter of the insect suction pipe is formed in the middle of the cryopreservation pipe cover, and the insect suction pipe is longer than the cryopreservation pipe and arranged in the cryopreservation pipe; the bottom of the cryopreservation pipe is connected with an air exhaust gun through a rubber sleeve head and an air pipe. The device is easy to assemble and convenient to carry, different medicaments are tested by replacing the cryopreservation tube, the effect of comprehensive and accurate determination is achieved, insect suction and testing are completed in the same device, transfer is not needed, manual errors are reduced, and a more accurate determination result is obtained.

In one aspect, the disclosure relates to a system comprising a plurality of microposts protruding from a membrane opposite a solid substrate, wherein the system can be pneumatically actuated to bring the microposts closer to the solid substrate. Also disclosed herein are methods of constructing the system, methods of performing digital bioassays using the disclosed system, and noninvasive methods for detecting diseases and for monitoring the progress of disease treatments using the disclosed system. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

A microfluidic device for bioassays, including: a microfluidic channel for guiding a process liquid in a direction of fluid flow from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel via at least one region of interest for bioassays within the microfluidic channel; and a bubble deflection structure arranged in the microfluidic channel and adapted to deflect gas bubbles entering the microfluidic channel via the fluid inlet, such that the gas bubbles are guided through the microfluidic channel along at least one bubble path formed outside the region of interest, while allowing process liquid entering the microfluidic channel via the fluid inlet to flow through the region of interest.

Embodiments of the present disclosure are directed to methods, systems and devices, for precise and reduced spot-size capabilities using a laser to alter surfaces without chemical treatment, chemical waste, or chemical residues is provided for microfluidic systems (e.g., lab-on-a-disk, for example). In some embodiments, hydrophobic and super-hydrophilic areas can be created on surfaces in the same material at different areas and positions merely by using different laser settings (e.g., spot size, wavelength, spacing, and / or pulse duration). Accordingly, capillary forces that are a recurrent issue in a microfluidic devices (e.g., a centrifugal microfluidic disk) can be controlled for practical applications, including, for example when users handle the disks and insert a sample, the moment the substrate / device (e.g., disk) is placed in a system (e.g., a centrifugal system), capillary forces can take place and move the fluids, which becomes a problem for sequential bioassays taking place in substrate / device (e.g., disk). Thus, in some embodiments, the systems, devices and methods increase fluid control in microfluidic devices.

This application relates to the technical field of bioassay, specifically disclosing a combined detection kit for pepsinogen I and pepsinogen II and its application. The disclosed combined detection kit for pepsinogen I and pepsinogen II includes: a sample pad, a conjugate pad, a coating membrane, and an absorbent pad; the coating membrane is an NC membrane coated with antibody; the conjugate pad is prepared by pretreating and drying a fiber membrane, followed by spraying time-resolved fluorescent microspheres labeled with antibody; the pretreating solution is a solution of pH 8.0-8.4 + 8-12mM BS + 0.8-1.2% BSA + 0.8-1.2% PEG4000 + 0.02-0.03% surfactant + 4-6% sucrose. Using the detection kit and method provided in this application for the combined detection of pepsinogen I / pepsinogen II exhibits high sensitivity, accuracy, and repeatability.

The present disclosure relates to methods of performing biological assays. The method includes determining a cumulative value of light intensity resulting from a reaction between a plurality of luminescent reagents and a test sample. Based on a knowledge base, the presence and / or concentration of a substance corresponding to a variety of bioassays may be determined. The knowledge base is prepared by determining the intensity of light resulting from multiple reactions between a plurality of luminescent reagents of different concentrations and a test sample of a human body. The knowledge base includes slope values at different points in time obtained from a temporal plot of light intensity. The knowledge base further includes a value of light intensity when a point in time in the time graph is zero and a total value of light intensity at different points in time.

The present invention pertains to the field of pest control. In particular, the invention pertains to bioassays to measure the response of insect cells to toxinsm. Furthermore, the invention pertains to methods for identifying receptors which play a role in the interaction of insecticidal toxins and insect cells. The invention thus also pertains to the identification of toxins having an activity against an insect cell, and to toxins that interact with receptor molecules.

This invention discloses an internal reference gene suitable for miRNA detection, along with related products and applications, relating to the field of bioassay. Through experimental testing and data analysis using different methods on miRNAs in a fever control group and KD patients, this invention discovered that the microRNA miR-30d-5p can serve as a standardized internal reference gene for the detection of platelet microRNAs in Kawasaki disease, exhibiting good stability and accuracy.

This utility model relates to the field of bioassay technology and discloses a layered multi-channel pest bioassay analysis device, including a main body assembly, a driving assembly on the top of the main body assembly, an installation assembly inside the main body assembly, a connecting assembly on the side of the installation assembly, and an auxiliary assembly inside the main body assembly. The main body assembly includes a box, with an opening at the top of the box. A channel tube is fixedly installed inside the box, and a layered chamber is formed inside the box. A gear rotates to drive a rack, which in turn drives a moving block. The internal groove of the moving block is adjusted to connect the corresponding channel tube and the opening, thereby applying multi-component layered and multi-channel pest assays. This allows for continuous pest guidance, avoiding the need to pause guidance during pest analysis, resulting in faster assay efficiency and more stable and accurate pest assays.

The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.

The present invention provides an integrated microfluidic-based device for establishing a wound infection in vivo model suitable for high throughput bioassay such as potential drug screening, in vivo dosing optimization, host-microbe or microbe-microbe interactions under the influence of specific agents of interest, and studying regulatory mechanisms of certain inflammatory diseases relating to or arising from the wound infection. The present invention allows direct qualitative and quantitative assessments of specific markers expressed due to the wound infection by one or more microbes devoid of cell sorting, isolation, or labelling as in other conventional in vivo models or methods.

The invention discloses a biological assay method for evaluating the influence of gene editing wheat on aphids, and belongs to the technical field of safety evaluation of gene editing crops. The bioassay method comprises the following steps: selecting a single wheat seedling after wheat seedlings emerge, wrapping the stem base of the single wheat seedling with wet absorbent cotton, fixing the stem base in a tube, then inoculating single-head wing-free adult aphids on the wheat seedling, removing the adult aphids and redundant young aphids after the adult aphids produce young aphids for the first time, and only retaining one young aphid. And from the beginning of the first-stage aphid production, observing once under a stereoscopic microscope every day, recording the growth and development conditions of the aphids, and removing aphid slough and dead individuals in time. And evaluating the influence of the gene editing wheat on the aphids by taking the survival rate of the aphids on the tenth day, the early aphid production stage, the life and the aphid production quantity as evaluation indexes. The method is wide in applicability and can be effectively used for evaluating the influence of various kinds of gene editing wheat on various aphids such as schizaphis graminum, sitobion avenae and rhopalosiphum padi.

The invention generally relates to high-throughput label-free enzymatic bioassays using desorption electrospray ionization-mass spectrometry (DESI-MS).

Described herein is an isolated biological polysaccharide compound. The biological polysaccharide compound may be characterised by being isolated and having: glycosyl linkages comprising 1:3 linked glucopyranosyl residue of 65-95%wt and 1:6 linked glucopyranosyl residue of 5-25%wt; and a purity of 85- 100% β-glucan; and a molecular weight of 0.5 to 2.2MDa; and a TNF-alpha cytokine response in a human bioassay that is at least 1.5 times greater than a negative control TNF-alpha cytokine response in a human bioassay; and being essentially insoluble in aqueous solutions. In at least one embodiment, methods are described of treating skin by topical application of a vehicle containing the isolated biological polysaccharide to a skin site such as a wound or burn. A method of manufacture is also described.

The present invention relates to prevention of undesired interference of gas bubbles with substances / objects of interest in a microfluidic system. In one aspect the invention provides a microfluidic device (1) for bioassays, comprising: - a microfluidic channel for guiding a process liquid in a direction of fluid flow (F) from a fluid inlet of the microfluidic channel to a fluid outlet of the microfluidic channel via at least one region of interest (16) for bioassays within the microfluidic channel; - a bubble deflection structure (10) arranged in the microfluidic channel and adapted to deflect gas bubbles (20) entering the microfluidic channel via the fluid inlet, such that said gas bubbles (20) are guided through the microfluidic channel along at least one bubble path (22) formed outside the region of interest (16), while allowing process liquid entering the microfluidic channel via the fluid inlet to flow through the region of interest (16).

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating beet armyworm, the active ingredient of the pesticide composition is formed by binary compounding of dichlorothiopyrazine and a compounding factor according to the mass ratio of (1-100): (100-1), wherein the compound factor is tetratolfenpyrad or chlorantraniliprole, and the compound factor is tetratolfenpyrad or chlorantraniliprole. According to the invention, the dichlorothiopyrazine is reasonably compounded with the compounding factor tolfenpyrad or chlorantraniliprole, and an indoor bioassay test shows that the compounded composition has a synergistic effect on beet armyworms, so that the control effect on the beet armyworms can be improved, and the dosage of the pesticide is reduced; and compared with the single use of dichlorothiopyrazine, the drug resistance risk can be reduced, and the service life can be prolonged.