45 results about "Spider mite" patented technology

The invention discloses a method for ecologically controlling pests in a citrus orchard, which comprises the following steps of: preparing mint, salvia officinalis and ageratum conyzoides in a ratio of 1: 1: 1 in the middle of the citrus orchard to form a strip-shaped or chessboard-shaped layout with the coverage area of 30-50%, and synergistically repelling the pests and attracting natural enemies by utilizing plant volatile matters; the method specifically comprises the steps that an intercropping belt is arranged at the position 1 m away from citrus trees, the belt width is 2 m, and the plant row spacing is 30 cm * 40 cm; citrus field planting is conducted, transplanting is conducted in spring of the next year, organic fertilizer and regular pruning and covering (three times every year, soil covering is conducted after smashing, and wood vinegar is added) are combined; mint releases menthol, salvia officinalis releases eucalyptol to expel parasites, ageratum conyzoides attracts predatory mites to control red spiders, and mineral oil and a yellow sticky card are supplemented for prevention and control; aiming at facility cultivation, LED supplementary lighting (red light: blue light: white light = 3: 1: 1) and fan diffusion volatile matters are additionally arranged. The use of chemical pesticides is reduced by 40% or above, the pest population density is reduced by 50%-70%, meanwhile, the diversity of soil organic matter and microorganisms is improved, and the method is suitable for open-air and greenhouse citrus orchards.

The present invention provides the use of 1-methoxyindole or its derivatives in repelling and / or killing pests, belonging to the field of pest control technology. The active ingredient provided by the present invention is 1-methoxyindole (1-MOI), which is found in Metarhizium robertsiense. When applied as a repellent, the concentration in a plane is 0.127 μg / cm 2 1-methoxyindole can repel a variety of ants, termites, and ticks, and the higher the 1-methoxyindole content, the stronger the repellent effect; when used as an insecticide, the concentration in the air is 0.14μg / cm 3 The 1-methoxyindole can kill a variety of ants, termites, ticks, thrips, mosquitoes, as well as whiteflies, brown planthoppers, spider mites and other pests, and the higher the concentration of 1-methoxyindole, the stronger the killing effect.

The invention belongs to the technical field of gene engineering, and particularly relates to application of TuPK39 protein in stimulating plant defense reaction. The invention finds that the TuPK39 protein has a new application of stimulating plant defense reaction, and tests find that the TuPK39 protein can cause plant cell necrosis reaction, stimulate plant reactive oxygen bursting and stimulate expression of plant defense genes, and provides research basis and technical support for subsequent prevention and control research of pests including tetranychid mites.

The application belongs to the technical field of medicine synthesis, and particularly relates to a steroid and 3,4 dihydropyrrole pyrazinone derivative and a synthesis method and application thereof. The derivative has a chemical structure as described in formula (I) or formula (II). The application first proposes a series of novel steroid and 3,4 dihydropyrrole pyrazinone derivatives. In addition, the application also proposes a synthesis method of the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative is obtained through a series of reactions based on dehydroepiandrosterone. The product prepared by the synthesis method has high yield and is easy to separate, and is the optimal method for preparing the steroid and 3,4 dihydropyrrole pyrazinone derivative. The steroid and 3,4 dihydropyrrole pyrazinone derivative provided by the application is confirmed by biological determination to have good toxic killing activity on aphids, spider mites and oriental armyworm insects, and can be applied to plant pest control.

The invention belongs to the technical field of plant protection and biological prevention and control, and particularly relates to a biological preparation for efficiently preventing and controlling red spiders. The biological agent for efficiently preventing and controlling red spiders, provided by the invention, is prepared from the following raw materials in parts by mass: 5 to 10 parts of lactic acid, 10 to 15 parts of ethanol, 8 to 15 parts of chitosan, 12 to 25 parts of marigold extract, 3 to 8 parts of clove extract, 1 to 3 parts of cationic surface active agent, 1 to 3 parts of VC and 30 to 60 parts of deionized water. The invention has a triple action mechanism of charge repulsion, biological inhibition and physical plugging, and can efficiently prevent and control red spider pests.

The invention discloses fused dsRNA for preventing and treating pest mites and application of the fused dsRNA, and belongs to the technical field of agricultural biology. The fusion dsRNA is dsfusion 1 or dsfusion 2, and the fusion dsRNA is dsfusion 1 or dsfusion 2. The invention provides two kinds of artificially synthesized fused dsRNAs (dsfusion 1 and dsfusion 2) targeting pest mite ecdysis hormone signal genes ECR-HR3-HR4 and ECR-HR3-E74, and experiments prove that the two kinds of fused dsRNAs have good interference efficiency and can be used for death of pest mites by inhibiting ecdysis of the pest mites; various pest mites can be effectively prevented and treated, but natural enemy predatory mites are not influenced; and the LC50 value is low, so that the prevention and treatment potential at low concentration can be improved. Therefore, the invention provides a new method for preventing and treating pest mites, and the fused dsRNA has a wide application prospect.

The application belongs to the technical field of medicine synthesis, and particularly relates to quinolinone derivatives, a synthesis method and application thereof. The quinolinone derivatives have a chemical structure as shown in formula (I). A series of novel quinolinone derivatives are first proposed in the application. In addition, the application further proposes a synthesis method of the quinolinone derivatives. The quinolinone derivatives are obtained through a series of reactions based on an indanone with different hydroxyl positions as a basic raw material. The product prepared by the synthesis method has high yield and is easy to separate, and the synthesis method is the optimal method for preparing the quinolinone derivatives. The application further provides a new use of the quinolinone derivatives. Biological tests prove that the quinolinone derivatives have good toxic killing activity on piercing-sucking insects such as aphids, armyworms, spider mites, rice planthoppers and whiteflies, and can be applied to plant pest control.

The invention discloses an endophyte for inhibiting corn spider mites. The invention provides bacillus velezensis which is bacillus velezensis 2025-BC248L1-1, and the preservation number of the bacillus velezensis 2025-BC248L1-1 is CGMCC (China General Microbiological Culture Collection Center) No. 33929. The invention also provides any one of the following applications of the bacillus velezensis or the culture thereof: A1) preventing plant diseases and insect pests; a2) treating plant diseases and insect pests; and A3) inhibiting phytopathogen. The endophyte with the activity of antagonizing the corn pathogenic bacteria is separated from corn leaves, the classification status of the endophyte is accurately determined by a molecular identification method, and the endophyte is identified as the bacillus. The endophyte is subjected to a biocontrol experiment of field endophyte liquid on spider mites, a new microbial resource is provided for biological control of corn diseases, and the endophyte is expected to be developed into a biological control preparation, so that the use of chemical pesticides can be reduced, and the environmental pollution is reduced.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

This invention belongs to the field of pharmaceutical synthesis technology, specifically relating to indomethacin derivatives, their synthesis methods, and applications. The derivatives possess the chemical structures described in formulas (I), (II), and (III). This invention is the first to propose a series of novel indomethacin derivatives. Furthermore, this invention also proposes a synthesis method for indomethacin derivatives, using 5-hydroxy-1-indanone as a base material, to obtain the indomethacin derivatives of this invention through a series of reactions. This synthesis method yields high-yield products that are easily separated, making it the optimal method for preparing the indomethacin derivatives of this invention. Bioassays have confirmed that the indomethacin derivatives provided by this invention exhibit good toxic activity against aphids, spider mites, and armyworms, and can be applied to the control of plant pests.

The utility model relates to a biological assay device for arthropods, and discloses a device for carrying out biological assay on spider mites on living plant leaves, which comprises a base (1), a telescopic device (2) and a storage platform (3). A fixing hole (11) is formed in the base (1); the telescopic device (2) is connected with the base (1) through a bolt, the telescopic device (2) comprises an outer pipe (22) and an inner pipe (21), and the outer pipe (22) is provided with a fixing bolt used for fixing the relative position of the inner pipe (21); the storage platform (3) is fixedly connected with the upper part of the inner pipe (21); the utility model has the advantages that the leaf is not easy to damage, the system stability is good, the position of the culture dish is adjustable, the whole device is easy to move, the observation is easy, and the like, and can better complete the biological assay test of the tetranychid on the living plant leaf.

The present invention provides a chitin synthesis inhibitor compound-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is buprofezin, lufenuron, cyromazine, diafenthiuron, diflubenzuron, chlorbenzuron, hexaflumuron or chlorfluazuron, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially brown planthopper, beet armyworm or liriomyza sativae. The invention verifies that the binary composition of the inhibitor insecticide synthesized from the flufenutraniliprole and the chitin has a remarkable synergistic effect, effectively improves the control effect, reduces the dosage and expands the insecticidal spectrum. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I).

The invention provides a Chinese beetle pyemotes skin necrosis toxin gene as well as an encoding protein and application thereof, and belongs to the technical field of biology. The toxin gene provided by the invention comprises the following nucleotide sequences: (a) a nucleotide sequence as shown in SEQ ID NO: 1; (b) a nucleotide sequence as shown in SEQ ID NO: 2; (c) a nucleotide sequence as shown in SEQ ID NO: 3; (d) a nucleotide sequence as shown in SEQ ID NO: 4; and (e) a nucleotide sequence having at least 95% sequence consistency with at least one of the nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. The pyemotes sinensis toxin protein provided by the invention is derived from natural toxin protein and has good insecticidal activity on spodoptera frugiperda.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention provides a pesticide composition containing a pyrethroid compound, which consists of a compound with a structural formula (I) and a second effective component, the second effective component is bifenthrin, beta-cyhalothrin, fenpropathrin, beta-cyhalothrin, beta-cyhalothrin, beta-cyhalothrin, cypermethrin, cyfluthrin, deltamethrin, ethofenprox, fenvalerate or cis-cypermethrin, and the weight ratio of the bifenthrin, the beta-cyhalothrin, the fenpropathrin, the fenvalerate and the cis-cypermethrin is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially phyllotreta striolata, ostrinia nubilalis or tetranychus cinnabarinus. The invention verifies that a remarkable synergistic effect can be obtained by compounding the flufenoxanil and the pyrethroid insecticide in a proper proportion, the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are realized, and the composition has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle and tetranychid mites, and has a good control effect on the target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis. (I).

The invention discloses a canopy scale cotton spider mite damage degree detection method and device, a medium and a product, and relates to the field of plant damage detection.The method comprises the steps that two leaf scale cotton spider mite damage degree detection models are constructed based on ResNet1D and MobileNet1D models respectively; adopting two leaf scale tetranychus gossypii damage degree detection models to predict leaf scale tetranychus gossypii damage degrees based on the obtained image color and texture features and the average spectrum, and obtaining color texture and spectrum key waveband features in the prediction process; and adopting the constructed three-branch feature fusion network model to obtain a canopy scale cotton spider mite damage degree detection result based on the spectrum key waveband features, the color texture key features and the RGB image. According to the method, potential association among different modal features can be deeply mined, and automatic and accurate detection of the damage degree of the cotton spider mites in the natural environment is realized.

The invention relates to agriculture. Liquid complex fertilizer contains colloidal silver, macroelements and microelements in the form of salts and metal-ethylenediaminetetraacetic acid chelates, additionally contains sodium tallow amphoteric polycarboxyglycine, polyvinylpyrrolidone, structured water formed by preliminary addition of shungite. All components are added in certain proportions. The invention allows to increase the following properties: acaricidal activity against common spider mites, insecticidal activity against common potato aphids, antiviral activity against tomato yellow leaf curl virus, and stability of the fertilizer as a colloidal system.

Compounds of formula (II) wherein A, B, G, W, X, Y and Z may have the meanings given in the description are well suited for treating ground / growth substrates against animal pests such as insects and / or red spider mites by ground irrigation or drip or immersion application.

The application belongs to the field of fine chemical industry, and particularly discloses a modified eugenol mixture for contact killing of spider mites and a preparation method thereof. The modified eugenol mixture is prepared by addition reaction of nonamethyltrihydrogen pentasiloxane, allyl polyether, eugenol and olefin under the condition of a platinum catalyst at normal pressure to obtain a novel structure siloxane grafted with eugenol; and then the product is mixed with a double-branched ethoxylated siloxane and a rhamnolipid surfactant according to a mass ratio to obtain the modified eugenol mixture for contact killing of spider mites. The novel structure siloxane is a novel eugenol compound for contact killing of spider mites, and the long-chain alkyl on the organic silicon chain can dissolve the sebum layer of the mites, and at the same time, the eugenol can penetrate below the sebum layer due to the low surface tension of silicon, so that the contact killing effect is achieved.

The invention relates to a pesticide composition containing a nicotine compound and application of the pesticide composition. The pesticide composition is prepared from flufenican amide, acetamiprid, thiamethoxam or clothianidin and the like. The pesticide composition has an obvious synergistic effect, the usage amount of pesticides is reduced, the residual amount of the pesticides on crops is reduced, and the environmental friendliness is enhanced. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae.

The invention discloses an AvHel31B target gene for preventing and treating tetranychus crataegus, application of the AvHel31B target gene, dsRNA synthesized by the AvHel31B target gene and application of the dsRNA. The nucleotide sequence of the AvHel31B target gene is as shown in SEQ ID NO. 1, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2; after the AvHel31B is interfered, the egg laying amount of the tetranychus crataegus is obviously reduced, and the survival rate is obviously influenced due to abnormal growth and development, so that the prevention and treatment aim is achieved; the method is convenient to operate, good in effectiveness and sensitivity and high in insecticidal efficiency, has the advantages of being environmentally friendly and the like, and has a good application prospect.

The application belongs to the technical field of pesticide mite killing, and discloses a pesticide composition and application thereof. The pesticide composition comprises active ingredient A and active ingredient B. The active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl) sulfide. The active ingredient B is any one of spirodiclofen or bifenazate. The mass ratio of the active ingredient A to the active ingredient B is 1:52-45:1. The pesticide composition has good control effect on citrus leaf mite and two-spotted spider mite, is fast-acting, and has long effective period.

The present application relates to a kind of synergistic adjuvant for improving the prevention and treatment effect of red spider and its preparation method and application, the dosage form of the adjuvant is emulsifiable concentrate, the adjuvant includes the following mass content components: eggshell breaking agent 1.0-50.0%, penetrant 10.0-50.0% and emulsifier 5.0-20.0%, solvent is supplemented to 100.0%;The eggshell breaking agent is the mixture of one or two of ethylenediamine oleate and C13 isopropyl alcohol amide in any ratio;The penetrant is alpha-terpineol;The solvent is the mixture of one or two of 4-isopropyltoluene and ethanol in any ratio.Compared with prior art, the present application can improve the physicochemical properties of pesticide solution, reduce the surface tension of pesticide, improve the penetration and spreading property and leaf retention of pesticide, the eggshell breaking agent has good wax dissolving and dewaxing performance, effectively improves the prevention and treatment effect of pesticide on red spider mite and egg.

The application discloses a corn spider mite resistance related molecular marker and application thereof. The application provides application of a substance for detecting the genotype of a SNP site Zm000011d042337@161,938,641 in a corn genome in at least one of the following: A1) identifying or assisting in identifying corn spider mite resistance; A2) breeding corn with high spider mite resistance; the SNP site Zm000011d042337@161,938,641 is the 23rd site in sequence 2. The application firstly finds two genes Zm000011d042333 and Zm000011d042337 related to mite resistance in corn, and identifies the mite resistance of corn according to the genotype of the SNP site on the gene, which creates a basis for breeding or screening resistant corn breeding resources.

The invention provides a primer combination for multiplex PCR (polymerase chain reaction) detection of three spider mites and application of the primer combination, and belongs to the technical field of molecular biology. The primer combination disclosed by the invention is shown as SEQ ID NO.1-SEQ ID NO.6. The multiple PCR method established by the invention can be used for simultaneously realizing the rapid identification of the starting tetranychus sex-point, the eutetranychus orientalis and the red mites and the rapid detection of the three tetranychus mites in the intestinal tracts of the natural enemies in the same reaction system, and has good specificity and sensitivity, and the primer combination disclosed by the invention can be used for simultaneously realizing the rapid identification of the starting tetranychus sex-point, the eutetranychus orientalis and the red mites. The whole process comprises total DNA extraction, PCR amplification and electrophoretic analysis, and can be completed within 8 hours, so that the detection time is shortened, the detection efficiency is improved, and the sensitivity is high. The multiplex PCR detection method has the advantages of rapidness, time saving, cost saving and the like when being used for detecting the three kinds of tetranychid mites in the field, and has a wide application prospect.

This invention discloses a traditional Chinese medicine compound preparation for the green control of citrus red spider mites, comprising principal, assistant, adjuvant, and guiding herbs: the principal herbs include: 15-20 parts of Veratrum nigrum and 15-20 parts of Stemona japonica; the assistant herbs include: 10-15 parts of Trichosanthes kirilowii, 10-15 parts of Melia toosendan, and 10-15 parts of Zingiber officinale; the adjuvant herbs include: 8-12 parts of Cnidium monnieri, 8-12 parts of Zanthoxylum bungeanum, and 8-12 parts of Ginkgo biloba; the guiding herbs include: 5-10 parts of Gleditsia sinensis, 5-8 parts of Mentha haplocalyx, and 5-8 parts of Glycyrrhiza uralensis. In the field of biological control of agricultural pests, the raw materials of traditional Chinese medicine are locally grown medicinal herbs with no chemically synthesized pesticide residues. They have low toxicity to mammals, birds, bees, and the main natural enemies of citrus (adult predatory mites), and meet the standards of organic agriculture and green food production. The compound preparations are formulated according to the "principal, assistant, adjuvant and guide" principle to exert a synergistic effect, and have comprehensive effects of contact killing, stomach poisoning, repellency, and inhibition of egg laying and hatching. The field control efficacy can reach more than 89%, and the rapid effect and the duration of effect (10-15 days) are balanced.

The invention relates to an insecticidal composition, which contains a compound as shown in formula (I), which is used as a first active ingredient, and one or more of chlorfenapyr, spirodiclofen, bifenthrin and flufenoxuron, which is used as a second active ingredient, and the weight ratio of the first active ingredient to the second active ingredient is (50: 1)-(1: 50). The insecticidal composition disclosed by the invention particularly has an excellent control effect on tetranychid mites. .

FIELD: agriculture.SUBSTANCE: method for breeding the predatory mite Neoseiulus reductus involves the use of a substance and a feed mite for its reproduction, wherein wheat plants are used as the substance, and the spider mite Oligonychuspratensis is used as the feed mite, the process is carried out by growing wheat hydroponically for 8–9 days until the 2-leaf phase, followed by populating the wheat plants with the Banks grass mite Oligonychuspratensis at a rate of 10 females per plant and releasing the predatory mite Neoseiulusreductus onto the wheat plants in the third leaf phase at a rate of 10 females per plant, maintaining the resulting mite composition at a temperature of 25–27 °C and air humidity of 70–80% until the tillering phase and the collection of predatory mites Neoseiulusreductus.EFFECT: creation of an effective method for breeding the predatory mite Neoseiulusreductus of the Phytoseiidae family, which makes it possible to obtain a high number of the predatory mite Neoseiulusreductus.1 cl, 3 tbl, 2 ex

The invention discloses corn leaf endophyte and application thereof in inhibition of corn spider mites. The invention provides bacillus velezensis, the bacillus velezensis is bacillus velezensis 2025-FCW119-1M2, and the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.33928. The bacillus velezensis provided by the invention is a bacillus velezensis strain. The invention also provides an application of the strain in any one of the following: A1) preventing plant diseases and insect pests; a2) treating plant diseases and insect pests; and A3) inhibiting phytopathogen. The endophyte with the activity of antagonizing the corn pathogenic bacteria is successfully separated from the corn leaves and is identified as the bacillus, a new microbial resource is provided for biological prevention and control of corn diseases, and the endophyte is expected to be developed into a biological prevention and control preparation, so that the use of chemical pesticides can be reduced, and the environmental pollution can be reduced.