119 results about "Plutella" patented technology

The invention belongs to the technical field of pesticides, and particularly relates to a substituted benzisoxazole compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein Z1, Z2, Z3, Z4 and Z5 independently represent N or CR; r independently represents hydrogen, halogen, alkyl, alkenyl or the like; x represents an alkyl group, a haloalkyl group, an alkenyl group or the like; y represents alkyl. The compound has excellent prevention and treatment effects on pests such as spodoptera frugiperda, armyworm, plutella xylostella and prodenia litura.

The invention provides an insecticide composition, an insecticide preparation and preparation and application of the insecticide composition and the insecticide preparation, and relates to the technical field of pesticides. The pesticide composition provided by the invention comprises a component A and a component B, the component A is (1S, 2R, 4S)-1, 3, 3-trimethyl bicyclo [2.2. 1] heptane-2-yl-4-(tert-butyl) benzoate, the component B is chlorantraniliprole, tetrachlorantraniliprole or emamectin benzoate, and the mass ratio of the component A to the component B is 1: (0.25-4). The insecticide composition provided by the invention has relatively high toxicity to lepidoptera pests such as cotton bollworms, plutella xylostella and corn borers, has a remarkable synergistic effect, can remarkably reduce the dosage of chlorantraniliprole, tetrachlorantraniliprole and emamectin benzoate, realizes application reduction and synergy of pesticides, and delays the development of pest resistance to drugs; and all the components are low-toxicity and low-residue compounds and are easy to degrade in the environment.

The invention relates to the technical field of pesticides, and discloses an isoxazoline compound, a preparation method and application thereof, an agricultural composition and a method for preventing and treating invertebrate pests. The invention provides an isoxazoline compound with a structure as shown in a formula (I), or an agriculturally acceptable salt thereof, or a stereoisomer thereof. The isoxazoline compound and the agriculturally acceptable salt thereof provided by the invention show very high prevention and control effects on various pests, especially spider mites represented by tetranychus cinnabarinus, tetranychus urticae, tetranychus shennieri, panonychus citri and the like, and lepidoptera insects represented by plutella xylostella and the like; the acaricidal composition can be used for preventing and treating various pests and harmful mites, has efficient acaricidal and insecticidal activity, and has a good application prospect. # imgabs0 #

The invention relates to a composition for a pesticide, an insecticide and application of the insecticide, the composition comprises emamectin benzoate and a bamboo vinegar diluent, and the bamboo vinegar diluent is obtained by diluting bamboo vinegar with water. The insecticide comprises the composition for the pesticide, and the insecticide is applied to prevention and treatment of lepidoptera pests, mites, coleoptera pests and homoptera pests, especially to prevention and treatment of plutella xylostella. In the composition disclosed by the invention, the bamboo vinegar diluent is used as a synergist to improve the pesticide effect of the emamectin benzoate, so that the pesticide containing the composition can improve the effect of preventing and treating lepidoptera pests, mites, coleoptera pests and homoptera pests, particularly has a remarkable effect of preventing and treating plutella xylostella, and further has a remarkable effect of preventing and treating the emamectin benzoate. According to the insecticide, the dosage of the emamectin benzoate can be reduced, the drug resistance of pests can be overcome and delayed, and meanwhile, the risk levels of environmental pollution, pesticide residues and the like can be reduced.

The invention relates to a promoter PinhA1 and application thereof in guiding efficient expression of functional genes. The nucleotide sequence of the promoter PinhA1 is as shown in SEQ ID NO: 1. The strong promoter PinhA1 can be used for efficiently guiding the expression of the cry1Ac gene in the bacillus thuringiensis delta cdsR mutant. According to the invention, a strain delta cdsR (PinhA1-1Ac) which is used for guiding cry1Ac expression by PinhA1 and generating a large number of regular bipyramid crystal proteins is constructed, and the Cry1Ac yield of the strain delta cdsR (PinhA1-1Ac) and the insecticidal activity of the strain delta cdsR (PinhA1-1Ac) on plutella xylostella larvae are equivalent to those of a wild strain HD73. Results prove that the strong promoter PinhA1 has a good application prospect in the aspect of constructing a bacillus thuringiensis engineering strain, and a new strategy is provided for improving crystal protein or other insecticides in future agricultural production.

The invention discloses a temperature response type emamectin benzoate-nitenpyram co-delivery liposome nano pesticide and a preparation method thereof. The compound emamectin benzoate and nitenpyram tablet is prepared from the following raw materials: 10%-40% of emamectin benzoate, 10%-40% of nitenpyram, 15%-50% of hydrogenated lecithin, 2%-35% of cholesterol and 1%-25% of a temperature-sensitive material, and the temperature-sensitive material is a decanoic acid-stearic acid binary fatty acid eutectic mixture. Compared with a conventional preparation, the temperature response type emamectin benzoate-nitenpyram co-delivery liposome nano pesticide provided by the invention shows excellent leaf surface wettability and lower toxicity to zebra fish. Indoor biological activity experiments show that the control effect of the nano-pesticide on the plutella xylostella is improved along with temperature rise and is consistent with the population growth trend of the plutella xylostella, and emamectin benzoate and nitenpyram in the nano-pesticide have a synergistic effect in the control of the plutella xylostella.

The invention discloses an application of labdane diterpenoid compounds in preparation of a pest repellent, and belongs to the field of pest repellent. The compound disclosed by the invention has insect antifeedant activity, and the insect antifeedant activity shows that the compound has antifeedant activity on plutella xylostella larvae and prodenia litura larvae. The compound provided by the invention can be separated and purified from natural plants, and has low toxicity, no residue, no pollution and no public nuisance for commercial crops such as various grains, melons and fruits, vegetables and the like.

The invention discloses a plant anti-lepidoptera pest related gene MIW1 and application thereof, and relates to the technical field of gene engineering, the MIW1 gene is derived from arabidopsis thaliana, the nucleotide sequence of the MIW1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of an encoding protein of the MIW1 gene is as shown in SEQ ID NO.2. The invention proves that the arabidopsis thaliana MIW1 gene can mediate RPX1 to resist plutella xylostella for the first time, and participates in pest resistance by promoting RPX1 protein degradation, so that the plant anti-lepidoptera pest related gene MIW1 can be used for preventing pests. As a new insect-resistant gene of a model plant, the arabidopsis MIW1 gene has high reference value and significance, and provides a new research thought for insect-resistant breeding of economic crops such as oilseed rape, corn, rice, sorghum and the like.

The invention relates to the field of chemical engineering and pesticides, in particular to an isoxazoline derivative containing dihydrazide as well as a preparation method and application of the isoxazoline derivative. The bishydrazide-containing isoxazoline derivative is a compound as shown in a formula (1). The novel isoxazoline derivative containing dihydrazide is obtained by combining an isoxazoline structure and a dihydrazide structure and carrying out special structural design, and the isoxazoline derivative containing dihydrazide has no cross resistance with existing insecticides, is relatively low in toxic and side effects, and can be applied to the field of pesticides. Meanwhile, the insecticidal composition has broad-spectrum insecticidal activity and an excellent insecticidal effect, and the problem that the pesticide resistance of pests such as spodoptera frugiperda and plutella xylostella is enhanced at present is effectively solved. Formula (1) # imgabs0 #

The invention relates to the technical field of spiro compounds, in particular to an 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound as well as a preparation method and application thereof. The invention provides the 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound, the compound shows excellent biological activity, and through test comparison, when the mass concentration is 0.1 mu g / mL, the insecticidal effect of the compound is increased by nearly 10% compared with that of a common insecticide chlorantraniliprole. It means that the compound shows a more efficient insecticidal effect on lepidoptera pests such as plutella xylostella and the like under a lower dosage, and cost reduction and synergism are realized. Due to the characteristic, the compound or the agronomically acceptable salt thereof shows important potential application value in the field of insecticides, and a new thought is provided for upgrading and updating of insecticides in the future.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The invention relates to the technical field of insecticides, and discloses a pesticide insecticide composition, which is prepared from flufenamid and spinosad, the mass ratio of the metafluzamide to the spinosad is (1 to 30): (1 to 30); the insecticide composition is used for preventing and treating insect pests of crops. According to the invention, the flufenamid and the spinosad are compounded and can generate a synergistic effect, so that the formed pesticide has a good insecticidal effect and a relatively large control spectrum, and particularly has a good control effect on plutella xylostella and eggplant thrips.

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The present invention relates to the preparation and application of difluoroalkene oxime ether derivatives. The general structural formula of the difluoroalkene oxime ether derivatives is shown in formula (I):. It has been found through research that the difluoroalkene oxime ether derivatives disclosed in the present invention have good biological activities against common and severely harmful agricultural pests / mites such as Plutella xylostella, Ostrinia furnacalis, Spodoptera frugiperda, and Tetranychus cinnabarinus. Therefore, they can be used as insecticidal / acaricidal candidate substances for the development of new, highly efficient, low-toxic, and environmentally friendly insecticides.

The invention belongs to the technical field of pesticides, and particularly relates to a heterocyclic substituted pyridine compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein R1, R2, R4, R5, R6, R8, R9 and R10 respectively and independently represent hydrogen, halogen, alkyl, alkenyl and the like; r3 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group or the like; and R7 represents halogen. The compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, beet armyworm, agrotis ypsilon, chilo suppressalis, plutella xylostella, brown planthopper, rhopalosiphum padi and the like.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention relates to the technical field of insect drug-resistant molecules. The invention provides a PxCYP6BG6 gene related to abamectin resistance of plutella xylostella and application of the PxCYP6BG6 gene. The nucleotide sequence of the PxCYP6BG6 gene is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The transcriptional activity of the gene is remarkably enhanced due to variation of a promoter sequence in a resistant strain, so that metabolism detoxification of abamectin is overexpressed and mediated. Tests prove that the sensitivity of plutella xylostella larvae to abamectin can be remarkably improved by inhibiting the expression of the gene. Molecular docking shows that the protein has high affinity with abamectin. The invention provides a key molecular marker for monitoring the abamectin resistance of the plutella xylostella, provides a theoretical basis and a technical scheme for developing an RNA pesticide or a novel insecticide synergist taking the gene as a target, and has important significance on resistance treatment and green prevention and control of the plutella xylostella.

The invention discloses application of a plutella xylostella calmodulin inhibitor in prevention and treatment of lepidoptera pests. According to the invention, an inhibitor Cyclosporin A capable of inhibiting the activity of Plutella xylostella calcineurin is used for feeding Plutella xylostella larvae, and it is found that Cyclosporin A can cause the death of Plutella xylostella larvae and reduce the survival rate of the larvae. In addition, the Cyclosporin A treatment can prolong the pupation time of the plutella xylostella and the eclosion time of pupae, greatly reduce the pupation rate and the eclosion rate, and also can obviously reduce the egg laying amount of female plutella xylostella and the hatching rate of eggs. On the basis, the plutella xylostella can be prevented and controlled by utilizing the substance capable of inhibiting the activity of the calmodulin of the plutella xylostella. Meanwhile, the calmodulin is relatively conservative in the lepidoptera insects, so that substances capable of inhibiting the activity of the calmodulin of the plutella xylostella can also be used for preventing and treating other lepidoptera insects. The invention provides a new target for prevention and treatment of lepidoptera pests, and is beneficial to green prevention and treatment of lepidoptera pests.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention relates to the field of chemical engineering and pesticides, in particular to an isoxazoline derivative containing sulfonamide as well as a preparation method and application of the isoxazoline derivative. The sulfonamide-containing isoxazoline derivative is a compound as shown in a formula (1). The novel sulfonamide-containing isoxazoline derivative is obtained by combining an isoxazoline structure and a sulfonamide structure and carrying out special structural design, and the sulfonamide-containing isoxazoline derivative has no cross resistance with the existing insecticide, has low toxic and side effects, and can be applied to the field of agricultural chemicals. Meanwhile, the insecticidal composition has broad-spectrum insecticidal activity and an excellent insecticidal effect, and the problem that the pesticide resistance of pests such as spodoptera frugiperda and plutella xylostella is enhanced at present is effectively solved. Formula (1) # imgabs0 #

The invention provides an abamectin B2a derivative as well as a preparation method and application thereof, and belongs to the technical field of pesticides, the structure of the abamectin B2a derivative is as shown in a formula I: # imgabs0 #, r2 is ortho-and para-substituted phenyl # imgabs1 # R3, R4 is H, F, Br, Cl and CF3 or # imgabs2 # R5 is methyl or ethyl. A series of compounds are obtained by modifying the 4 ''site of the abamectin B2a, and the compound is stable in structure, has special effects on armyworms, plutella xylostella, mites and underground nematodes, has a good control effect on resistant pests, is long in lasting period, and has important significance on effective utilization of the abamectin B2a.

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The invention discloses application of knockout or inhibition of an insect Kir2A gene in insect ovicidal, and belongs to the technical field of pesticide science. The invention provides an application of a gene for knocking out or inhibiting an insect Kir2A channel in insect ovicidal. By inhibiting the channel activity of the Kir2A or interfering the expression of the Kir2A gene, the egg abortion of the plutella xylostella is caused, and a new target is provided for developing an ovicide with a brand new action mechanism.

The invention provides a bacillus thuringiensis engineering strain for efficiently preventing and treating plutella xylostella, the engineering strain carries a nucleic acid fragment for preparing dsRNA, and the prepared dsRNA can interfere with the expression of a plutella xylostella mitogen activated protein kinase (MAP4K4) gene; the bacillus thuringiensis engineering strain is a Bt strain B-T-3 of which the preservation number is CCTCC (China Center for Type Culture Collection) No: M20252327. The engineering bacterium provided by the invention has a strong silencing effect on plutella xylostella MAP4K4 gene expression and shows high insecticidal activity on plutella xylostella larvae, the median lethal concentration on the plutella xylostella larvae is 2.3 * 10 < 6 > cfu / mL, and the activity is 3.2 times that of a wild strain. The result shows that the dsRNA and the Bt strain have synergistic toxicity.

The invention relates to the technical field of pesticides, and discloses an application method for preventing and treating cabbage plutella xylostella. The application method is a method for controlling cabbage plutella xylostella by spraying the chemical pesticide according to a certain concentration. The chemical pesticide is prepared from methoxyfenozide, tolfenpyrad, a synergist, an auxiliary agent and water; wherein the ratio of methoxyfenozide to tolfenpyrad is (2: 1)-(1: 2). The synergist is a seaweed extract, amino acid or polypeptide. The application method for preventing and treating cabbage plutella xylostella provided by the invention can effectively control the occurrence of pests, improve the utilization rate of chemical pesticides, improve the crop quality and regulate the soil nutrition.

The invention relates to the technical field of pesticide chemistry, and discloses a triazole ring-containing phenylisoxazole derivative as well as a preparation method and application thereof. The structural formula of the phenyl isoxazole derivative containing the triazole ring is # imgabs0. When the concentration of the phenyl isoxazole derivative containing the triazole ring is 50 mu g / mL, the phenyl isoxazole derivative containing the triazole ring shows good insecticidal activity on agricultural pests such as plutella xylostella, spodoptera frugiperda, cotton bollworm, corn borer and aphid, and therefore the phenyl isoxazole derivative containing the triazole ring has good insecticidal activity on agricultural pests such as corn borer and aphid. The triazole ring-containing phenylisoxazole derivative provided by the invention can be used as a pesticide active component to be applied to prevention and control of agricultural pests.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention provides a pesticide composition containing a pyrethroid compound, which consists of a compound with a structural formula (I) and a second effective component, the second effective component is bifenthrin, beta-cyhalothrin, fenpropathrin, beta-cyhalothrin, beta-cyhalothrin, beta-cyhalothrin, cypermethrin, cyfluthrin, deltamethrin, ethofenprox, fenvalerate or cis-cypermethrin, and the weight ratio of the bifenthrin, the beta-cyhalothrin, the fenpropathrin, the fenvalerate and the cis-cypermethrin is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially phyllotreta striolata, ostrinia nubilalis or tetranychus cinnabarinus. The invention verifies that a remarkable synergistic effect can be obtained by compounding the flufenoxanil and the pyrethroid insecticide in a proper proportion, the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are realized, and the composition has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle and tetranychid mites, and has a good control effect on the target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis. (I).