76 results about "Plutella" patented technology

The invention belongs to the technical field of pesticides, and particularly relates to a substituted benzisoxazole compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein Z1, Z2, Z3, Z4 and Z5 independently represent N or CR; r independently represents hydrogen, halogen, alkyl, alkenyl or the like; x represents an alkyl group, a haloalkyl group, an alkenyl group or the like; y represents alkyl. The compound has excellent prevention and treatment effects on pests such as spodoptera frugiperda, armyworm, plutella xylostella and prodenia litura.

The invention relates to the technical field of spiro compounds, in particular to an 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound as well as a preparation method and application thereof. The invention provides the 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound, the compound shows excellent biological activity, and through test comparison, when the mass concentration is 0.1 mu g / mL, the insecticidal effect of the compound is increased by nearly 10% compared with that of a common insecticide chlorantraniliprole. It means that the compound shows a more efficient insecticidal effect on lepidoptera pests such as plutella xylostella and the like under a lower dosage, and cost reduction and synergism are realized. Due to the characteristic, the compound or the agronomically acceptable salt thereof shows important potential application value in the field of insecticides, and a new thought is provided for upgrading and updating of insecticides in the future.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The invention belongs to the technical field of pesticides, and particularly relates to a heterocyclic substituted pyridine compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein R1, R2, R4, R5, R6, R8, R9 and R10 respectively and independently represent hydrogen, halogen, alkyl, alkenyl and the like; r3 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group or the like; and R7 represents halogen. The compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, beet armyworm, agrotis ypsilon, chilo suppressalis, plutella xylostella, brown planthopper, rhopalosiphum padi and the like.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention relates to the technical field of insect drug-resistant molecules. The invention provides a PxCYP6BG6 gene related to abamectin resistance of plutella xylostella and application of the PxCYP6BG6 gene. The nucleotide sequence of the PxCYP6BG6 gene is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The transcriptional activity of the gene is remarkably enhanced due to variation of a promoter sequence in a resistant strain, so that metabolism detoxification of abamectin is overexpressed and mediated. Tests prove that the sensitivity of plutella xylostella larvae to abamectin can be remarkably improved by inhibiting the expression of the gene. Molecular docking shows that the protein has high affinity with abamectin. The invention provides a key molecular marker for monitoring the abamectin resistance of the plutella xylostella, provides a theoretical basis and a technical scheme for developing an RNA pesticide or a novel insecticide synergist taking the gene as a target, and has important significance on resistance treatment and green prevention and control of the plutella xylostella.

The invention discloses application of a plutella xylostella calmodulin inhibitor in prevention and treatment of lepidoptera pests. According to the invention, an inhibitor Cyclosporin A capable of inhibiting the activity of Plutella xylostella calcineurin is used for feeding Plutella xylostella larvae, and it is found that Cyclosporin A can cause the death of Plutella xylostella larvae and reduce the survival rate of the larvae. In addition, the Cyclosporin A treatment can prolong the pupation time of the plutella xylostella and the eclosion time of pupae, greatly reduce the pupation rate and the eclosion rate, and also can obviously reduce the egg laying amount of female plutella xylostella and the hatching rate of eggs. On the basis, the plutella xylostella can be prevented and controlled by utilizing the substance capable of inhibiting the activity of the calmodulin of the plutella xylostella. Meanwhile, the calmodulin is relatively conservative in the lepidoptera insects, so that substances capable of inhibiting the activity of the calmodulin of the plutella xylostella can also be used for preventing and treating other lepidoptera insects. The invention provides a new target for prevention and treatment of lepidoptera pests, and is beneficial to green prevention and treatment of lepidoptera pests.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The invention discloses application of knockout or inhibition of an insect Kir2A gene in insect ovicidal, and belongs to the technical field of pesticide science. The invention provides an application of a gene for knocking out or inhibiting an insect Kir2A channel in insect ovicidal. By inhibiting the channel activity of the Kir2A or interfering the expression of the Kir2A gene, the egg abortion of the plutella xylostella is caused, and a new target is provided for developing an ovicide with a brand new action mechanism.

The invention provides a bacillus thuringiensis engineering strain for efficiently preventing and treating plutella xylostella, the engineering strain carries a nucleic acid fragment for preparing dsRNA, and the prepared dsRNA can interfere with the expression of a plutella xylostella mitogen activated protein kinase (MAP4K4) gene; the bacillus thuringiensis engineering strain is a Bt strain B-T-3 of which the preservation number is CCTCC (China Center for Type Culture Collection) No: M20252327. The engineering bacterium provided by the invention has a strong silencing effect on plutella xylostella MAP4K4 gene expression and shows high insecticidal activity on plutella xylostella larvae, the median lethal concentration on the plutella xylostella larvae is 2.3 * 10 < 6 > cfu / mL, and the activity is 3.2 times that of a wild strain. The result shows that the dsRNA and the Bt strain have synergistic toxicity.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention provides a pesticide composition containing a pyrethroid compound, which consists of a compound with a structural formula (I) and a second effective component, the second effective component is bifenthrin, beta-cyhalothrin, fenpropathrin, beta-cyhalothrin, beta-cyhalothrin, beta-cyhalothrin, cypermethrin, cyfluthrin, deltamethrin, ethofenprox, fenvalerate or cis-cypermethrin, and the weight ratio of the bifenthrin, the beta-cyhalothrin, the fenpropathrin, the fenvalerate and the cis-cypermethrin is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially phyllotreta striolata, ostrinia nubilalis or tetranychus cinnabarinus. The invention verifies that a remarkable synergistic effect can be obtained by compounding the flufenoxanil and the pyrethroid insecticide in a proper proportion, the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are realized, and the composition has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle and tetranychid mites, and has a good control effect on the target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis. (I).

The invention discloses a method for identifying plutella xylostella-resistant germplasm resources of cruciferous vegetable crops. The identification method comprises an identification device and an identification method, the identification device is provided with a breathable net on a cover body of a culture dish, agarose, filter paper and moisturizing cotton are combined to construct a proper feeding environment for the plutella xylostella, and a field feeding process is truly simulated under indoor conditions. On the basis of the device, an insect resistance identification method is established, three-instar larvae of plutella xylostella are inoculated and cultured regularly, and larva death number, weight change and leaf damage data are obtained; and further constructing a germplasm resource identification method for resisting the plutella xylostella of the cruciferous vegetable crops, and carrying out quantitative analysis and grade judgment on the insect resistance of the cruciferous vegetable crops by taking the larva death rate, the body weight inhibition rate and the leaf damage rate as evaluation indexes. The identification device and method cooperate with each other to form a standardized cruciferous vegetable crop insect-resistant resource identification system.

The application discloses an extract of Marchantia paleacea with insect antifeedant activity, a preparation method and application thereof, and belongs to the field of pest repellents. Radula complanata The application carries out research on insect antifeedant activity of Marchantia paleacea, extracts by heating and refluxing, extracts by different polar solvents, obtains petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase, and carries out screening on the antifeedant activity by using Plutella xylostella and Helicoverpa armigera, researches show that each phase extract of Marchantia paleacea contains insect antifeedant activity, especially the petroleum ether phase and the ethyl acetate phase have the highest antifeedant activity on Plutella xylostella, and a new idea is provided for biological control.

The present application belongs to the technical field of microorganisms, and discloses a new Talaromyces amestolkiae strain with broad-spectrum lepidopterous insecticidal activity and application thereof. The new strain is isolated from the pests of Spodoptera litura, and is named as Talaromyces amestolkiae GZTA01, which has been preserved in China Center for Type Culture Collection on January 6, 2026, and the strain preservation number is CCTCC NO: M2026033. The Talaromyces amestolkiae strain of the present application has a minimum lethal rate of 62.14% and a maximum lethal rate of 86.67% on Spodoptera litura, Myzus persicae, Plutella xylostella and Mythimna separata, and exhibits excellent broad-spectrum control potential on lepidopterous and hemipterous pests.

The application discloses a novel insect short neuropeptide sNPF analogue and application thereof in pests, and relates to the technical field of agriculture; the application provides a structural general formula of the novel insect short neuropeptide sNPF analogue, and the novel insect short neuropeptide sNPF analogue is applied to the prevention and treatment of aphids, thrips, Plutella xylostella and Spodoptera frugiperda. The terminal tyrosine Y, arginine R or leucine L residue is substituted and modified by using hydrophilic, hydrophobic, steric hindrance natural amino acids or unnatural amino acids and other groups on a lead compound, and the obtained novel insect short neuropeptide sNPF analogue can be used in the prevention and treatment of various pests.

The present invention belongs to the field of agricultural pest control and relates to a strain of Enterobacter asburiae Plutella xylostella (PxGF1) and its applications. The strain is classified and named Enterobacter asburiae PxGF1. The strain was deposited with the China Center for Type Culture Collection on January 10, 2023, with a deposit number of CCTCC NO: M2023056 at Wuhan University, Wuhan. Experiments have demonstrated that the strain PxGF1 of the present invention can improve the efficiency of Cry1Ac in killing Plutella xylostella. Furthermore, compared to feeding Plutella xylostella only with Cry1Ac protoxin, the pupation rate and pupal weight of the test insects were significantly reduced, and the intestinal tissue was severely damaged, making it impossible to observe intact intestinal epithelial cells. This suggests that PxGF1 promotes the killing ability of Cry1Ac against Plutella xylostella.

The invention discloses a flat calyx moss extract with insect antifeedant activity as well as a preparation method and application thereof, and belongs to the field of pest repellent. According to the invention, research on insect antifeedant activity is carried out on Radula Complanata (L.) Dumor, extraction is carried out by adopting a heating reflux method, then extraction is carried out by adopting solvents with different polarities to obtain a petroleum ether phase, an ethyl acetate phase, an n-butanol phase and a water phase, and the antifeedant activity is screened by using plutella xylostella and prodenia litura, so that the method for screening the antifeedant activity of Radula Complanata (L.) Dumor is provided for screening the antifeedant activity of Radula Complanata (L.) Dumor. Researches show that the extract of each phase of calyx tongue has insect antifeedant activity, especially the petroleum ether phase and the ethyl acetate phase have the highest antifeedant activity on plutella xylostella, and a new thought is provided for biological control.

The invention discloses application of enterobacter pseudohormaechei in preparation of an agricultural pest control agent, and belongs to the field of pesticide pest control. According to the invention, the fact that the enterobacter pseudohormaechei XWc1 strain has an obvious antifeedant effect on the plutella xylostella at each age is found for the first time. The enterobacter pseudohormaechei XWc1 strain can be used as an environment-friendly green pesticide to replace chemicals harmful to the environment such as chemical pesticides to prevent and control agricultural pests such as plutella xylostella and the like, a new biocontrol microbial strain and a prevention and control method strategy are provided for prevention and control of the agricultural pests, and the strain is harmless to the environment, does not affect human health and has a good application prospect. The biological control effect is good.

The invention provides an insecticidal composition containing flurala and bacillus thuringiensis G033A and application of the insecticidal composition. The equivalent volume ratio of flurala to bacillus thuringiensis G033A is 9: 1-1: 9 (converted into that the weight ratio of effective components is 1: 100-1: 31638), and preferably, the equivalent volume ratio is 5: 5, 6: 4, 7: 3, 8: 2 and 9: 1. The composition disclosed by the invention has an obvious synergistic effect, the use amount of a biological agent can be reduced, and the prevention and treatment cost is reduced; meanwhile, the use amount of chemical pesticides can be reduced, and the harm to the environment is reduced. The generation of drug resistance of lepidoptera pests such as plutella xylostella and cnaphalocrocis medinalis can be delayed.

The application provides a human-derived genetically engineered antibody with targeted simulation of Bt Cry1C toxin structure and insecticidal function and a high-activity mutant of the human-derived genetically engineered antibody, nucleotide sequences and amino acid sequences of the human-derived genetically engineered antibody are respectively shown as SEQ IN NO. 1 and SEQ IN NO. 2, nucleotide sequences and amino acid sequences of the mutant are respectively shown as SEQ IN NO. 3 and SEQ IN NO. 4; after the antibody and the mutant are expressed in prokaryotes, the LD50 of corresponding proteins to Plutella xylostella larvae is respectively 38.9% and 46.8% of the original Bt Cry1C toxin, the LD50 of corresponding proteins to cotton bollworm larvae is respectively 32.9% and 37.4% of the original Bt Cry1C toxin; the two can be respectively used as coating antigens in an IC-ELISA detection method to detect Bt Cry1C toxin with high sensitivity; the antibody and the mutant can be used as antigen analogue materials to replace the structure of Bt Cry1C toxin, not only can be used as new protein insect-resistant materials to have potential application value in green control of agricultural pests, but also can be used for detection of Bt Cry1C toxin residues in the environment and food samples, and have wide application value.

The invention discloses a granule virus microcapsule as well as a preparation method and application thereof, a plutella xylostella granule virus or codling moth granule virus is taken as a capsule core, polydopamine is taken as a wall material, a thermal protective agent is added, and the granule virus microcapsule is prepared by a spray drying method. According to the microcapsule insecticide for the plutella xylostella granulosis virus and the codling moth granulosis virus, provided by the invention, the viral granulosis is successfully wrapped by a wall material and a thermal protective agent maltodextrin, and the adhesive force on a glass slide and a plant leaf is remarkably enhanced; the plutella xylostella granulosis virus and codling moth granulosis virus microcapsule provided by the invention can effectively improve the anti-ultraviolet capability of a viral insecticide.

According to the bacillus thuringiensis and the application thereof in preventing and treating the plutella xylostella, the bacillus thuringiensis with specific activity on the plutella xylostella is screened, the types of insecticidal proteins of the bacillus thuringiensis are identified, and a new microbial resource is provided for biological prevention and treatment of the plutella xylostella. The preservation number of the provided bacillus thuringiensis B-T-3 strain is CCTCC (China Center For Type Culture Collection) No: M20252325. The high-toxicity bacillus thuringiensis obtained by screening has remarkable and excellent insecticidal activity on 2-instar and 3-instar larvae of plutella xylostella. Under the same treatment concentration, the corrected death rate is obviously higher than that of other isolated strains.

The invention relates to the technical field of preparation of isoxazoline derivatives, in particular to preparation and application of an isoxazoline derivative containing an oxime ether structure, the structural formula of the isoxazoline derivative containing the oxime ether structure is shown as a general formula I. In the formula, R1, R2 and R3 are hydrogen, halogen, cyano, nitro, trifluoromethyl, alkoxy or alkyl; r4 is hydrogen, halogen, cyano, nitro, C1-C6 alkyl, substituted benzyl, naphthenic base or heterocyclic ring; the isoxazoline derivative containing the oxime ether structure, disclosed by the invention, can be used for preventing and treating major lepidoptera agricultural pests, such as plutella xylostella, spodoptera frugiperda, ostrinia nubilalis, helicoverpa armigera, mythimna septemmina and the like. The isoxazoline derivative containing the oxime ether structure disclosed by the invention can be used for preventing and treating major lepidoptera agricultural pests, such as the plutella xylostella, the spodoptera frugiperda, the ostrinia nubilalis, the helicoverpa armigera, the mythimna septemmina and the like.

The application discloses an isoxazoline compound as shown in formula (I) and application thereof, wherein R1, R2 and R3 can be selected from halogen atoms, a cyano group, a nitro group, C1-C3 halogenated alkyl and the like; Q can be independently selected from A1-A6; L1 and L2 can be selected from a carboxyl group, C1-C6 alkoxycarbonyl, a C1-C8 alkyl group substituted by a hydroxyl group and the like; Z1 and Z2 can be selected from a hydrogen atom, a C1-C6 alkyl group, a C3-C5 cycloalkyl group or C(O)M, wherein M is a C1-C6 alkyl group, a C2-C6 alkenyl group and the like; the isoxazoline compound provided by the application has no cross resistance with existing insecticides, has the advantages of fast drug efficacy, low dosage and the like, and has excellent prevention and treatment effects on rice resistant Chilo suppressalis, Plutella xylostella, aphids and the like. The application further provides the isoxazoline compound as a salt acceptable to a pesticide, a composition and application.

The invention relates to the technical field of green prevention and control of insects, in particular to application of a plant extract in prevention and control of egg laying of plutella xylostella. According to the method, the spawning behavior of the plutella xylostella is controlled by adopting a push-pull comprehensive prevention and control mode through the stimulation or repelling effect of different plant extracts on the spawning behavior of the plutella xylostella, so that the maximum protection of target crops is realized.