49 results about "Plutella" patented technology

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The invention relates to a pesticide composition containing ecdysone compounds and application of the pesticide composition. The pesticide composition is composed of flufeniprole and methoxyfenozide or tebufenozide. The pesticide composition has an obvious synergistic effect, and the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, tryporyza incertulas, rice leaf folders, plant hoppers, prodenia litura, beet armyworms, plutella xylostella, cabbage caterpillars, aphids, aleyrodids, thrips and flea beetles.

The invention belongs to the technical field of pesticides, and particularly relates to a heterocyclic substituted pyridine compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein R1, R2, R4, R5, R6, R8, R9 and R10 respectively and independently represent hydrogen, halogen, alkyl, alkenyl and the like; r3 represents hydrogen, an alkyl group, a haloalkyl group, an alkenyl group or the like; and R7 represents halogen. The compound has excellent prevention and treatment effects on spodoptera frugiperda, armyworm, prodenia litura, beet armyworm, agrotis ypsilon, chilo suppressalis, plutella xylostella, brown planthopper, rhopalosiphum padi and the like.

The invention discloses application of a saponin enzymolysis product in prevention and treatment of plutella xylostella, and belongs to the technical field of biopesticides. According to the method, gleditsia sinensis saponin is degraded by using biological enzyme, and the obtained saponin degradation product has excellent food refusal, contact toxicity and stomach toxicity activity on plutella xylostella. The raw materials are obtained from natural plants, the preparation method is green and safe, the activity of degradation products is high, use of chemical pesticides can be reduced, degradation can be achieved in the natural environment, and a novel green prevention and control agent and technology can be provided for prevention and control of plutella xylostella.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention relates to the technical field of insect drug-resistant molecules. The invention provides a PxCYP6BG6 gene related to abamectin resistance of plutella xylostella and application of the PxCYP6BG6 gene. The nucleotide sequence of the PxCYP6BG6 gene is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The transcriptional activity of the gene is remarkably enhanced due to variation of a promoter sequence in a resistant strain, so that metabolism detoxification of abamectin is overexpressed and mediated. Tests prove that the sensitivity of plutella xylostella larvae to abamectin can be remarkably improved by inhibiting the expression of the gene. Molecular docking shows that the protein has high affinity with abamectin. The invention provides a key molecular marker for monitoring the abamectin resistance of the plutella xylostella, provides a theoretical basis and a technical scheme for developing an RNA pesticide or a novel insecticide synergist taking the gene as a target, and has important significance on resistance treatment and green prevention and control of the plutella xylostella.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The present invention provides an indoxacarb-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is indoxacarb, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of chilo suppressalis. Experiments prove that the binary composition obtained by compounding flufenican amide and indoxacarb in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The invention discloses application of knockout or inhibition of an insect Kir2A gene in insect ovicidal, and belongs to the technical field of pesticide science. The invention provides an application of a gene for knocking out or inhibiting an insect Kir2A channel in insect ovicidal. By inhibiting the channel activity of the Kir2A or interfering the expression of the Kir2A gene, the egg abortion of the plutella xylostella is caused, and a new target is provided for developing an ovicide with a brand new action mechanism.

The invention provides a bacillus thuringiensis engineering strain for efficiently preventing and treating plutella xylostella, the engineering strain carries a nucleic acid fragment for preparing dsRNA, and the prepared dsRNA can interfere with the expression of a plutella xylostella mitogen activated protein kinase (MAP4K4) gene; the bacillus thuringiensis engineering strain is a Bt strain B-T-3 of which the preservation number is CCTCC (China Center for Type Culture Collection) No: M20252327. The engineering bacterium provided by the invention has a strong silencing effect on plutella xylostella MAP4K4 gene expression and shows high insecticidal activity on plutella xylostella larvae, the median lethal concentration on the plutella xylostella larvae is 2.3 * 10 < 6 > cfu / mL, and the activity is 3.2 times that of a wild strain. The result shows that the dsRNA and the Bt strain have synergistic toxicity.

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

The invention provides a pesticide composition containing a pyrethroid compound, which consists of a compound with a structural formula (I) and a second effective component, the second effective component is bifenthrin, beta-cyhalothrin, fenpropathrin, beta-cyhalothrin, beta-cyhalothrin, beta-cyhalothrin, cypermethrin, cyfluthrin, deltamethrin, ethofenprox, fenvalerate or cis-cypermethrin, and the weight ratio of the bifenthrin, the beta-cyhalothrin, the fenpropathrin, the fenvalerate and the cis-cypermethrin is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention and control of agricultural and forestry pests, especially phyllotreta striolata, ostrinia nubilalis or tetranychus cinnabarinus. The invention verifies that a remarkable synergistic effect can be obtained by compounding the flufenoxanil and the pyrethroid insecticide in a proper proportion, the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are realized, and the composition has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle and tetranychid mites, and has a good control effect on the target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis. (I).

The invention discloses a method for identifying plutella xylostella-resistant germplasm resources of cruciferous vegetable crops. The identification method comprises an identification device and an identification method, the identification device is provided with a breathable net on a cover body of a culture dish, agarose, filter paper and moisturizing cotton are combined to construct a proper feeding environment for the plutella xylostella, and a field feeding process is truly simulated under indoor conditions. On the basis of the device, an insect resistance identification method is established, three-instar larvae of plutella xylostella are inoculated and cultured regularly, and larva death number, weight change and leaf damage data are obtained; and further constructing a germplasm resource identification method for resisting the plutella xylostella of the cruciferous vegetable crops, and carrying out quantitative analysis and grade judgment on the insect resistance of the cruciferous vegetable crops by taking the larva death rate, the body weight inhibition rate and the leaf damage rate as evaluation indexes. The identification device and method cooperate with each other to form a standardized cruciferous vegetable crop insect-resistant resource identification system.

The application discloses an extract of Marchantia paleacea with insect antifeedant activity, a preparation method and application thereof, and belongs to the field of pest repellents. Radula complanata The application carries out research on insect antifeedant activity of Marchantia paleacea, extracts by heating and refluxing, extracts by different polar solvents, obtains petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase, and carries out screening on the antifeedant activity by using Plutella xylostella and Helicoverpa armigera, researches show that each phase extract of Marchantia paleacea contains insect antifeedant activity, especially the petroleum ether phase and the ethyl acetate phase have the highest antifeedant activity on Plutella xylostella, and a new idea is provided for biological control.

The present application belongs to the technical field of microorganisms, and discloses a new Talaromyces amestolkiae strain with broad-spectrum lepidopterous insecticidal activity and application thereof. The new strain is isolated from the pests of Spodoptera litura, and is named as Talaromyces amestolkiae GZTA01, which has been preserved in China Center for Type Culture Collection on January 6, 2026, and the strain preservation number is CCTCC NO: M2026033. The Talaromyces amestolkiae strain of the present application has a minimum lethal rate of 62.14% and a maximum lethal rate of 86.67% on Spodoptera litura, Myzus persicae, Plutella xylostella and Mythimna separata, and exhibits excellent broad-spectrum control potential on lepidopterous and hemipterous pests.

The application discloses a novel insect short neuropeptide sNPF analogue and application thereof in pests, and relates to the technical field of agriculture; the application provides a structural general formula of the novel insect short neuropeptide sNPF analogue, and the novel insect short neuropeptide sNPF analogue is applied to the prevention and treatment of aphids, thrips, Plutella xylostella and Spodoptera frugiperda. The terminal tyrosine Y, arginine R or leucine L residue is substituted and modified by using hydrophilic, hydrophobic, steric hindrance natural amino acids or unnatural amino acids and other groups on a lead compound, and the obtained novel insect short neuropeptide sNPF analogue can be used in the prevention and treatment of various pests.

According to the bacillus thuringiensis and the application thereof in preventing and treating the plutella xylostella, the bacillus thuringiensis with specific activity on the plutella xylostella is screened, the types of insecticidal proteins of the bacillus thuringiensis are identified, and a new microbial resource is provided for biological prevention and treatment of the plutella xylostella. The preservation number of the provided bacillus thuringiensis B-T-3 strain is CCTCC (China Center For Type Culture Collection) No: M20252325. The high-toxicity bacillus thuringiensis obtained by screening has remarkable and excellent insecticidal activity on 2-instar and 3-instar larvae of plutella xylostella. Under the same treatment concentration, the corrected death rate is obviously higher than that of other isolated strains.

The invention relates to the technical field of preparation of isoxazoline derivatives, in particular to preparation and application of an isoxazoline derivative containing an oxime ether structure, the structural formula of the isoxazoline derivative containing the oxime ether structure is shown as a general formula I. In the formula, R1, R2 and R3 are hydrogen, halogen, cyano, nitro, trifluoromethyl, alkoxy or alkyl; r4 is hydrogen, halogen, cyano, nitro, C1-C6 alkyl, substituted benzyl, naphthenic base or heterocyclic ring; the isoxazoline derivative containing the oxime ether structure, disclosed by the invention, can be used for preventing and treating major lepidoptera agricultural pests, such as plutella xylostella, spodoptera frugiperda, ostrinia nubilalis, helicoverpa armigera, mythimna septemmina and the like. The isoxazoline derivative containing the oxime ether structure disclosed by the invention can be used for preventing and treating major lepidoptera agricultural pests, such as the plutella xylostella, the spodoptera frugiperda, the ostrinia nubilalis, the helicoverpa armigera, the mythimna septemmina and the like.

The invention relates to the technical field of green prevention and control of insects, in particular to application of a plant extract in prevention and control of egg laying of plutella xylostella. According to the method, the spawning behavior of the plutella xylostella is controlled by adopting a push-pull comprehensive prevention and control mode through the stimulation or repelling effect of different plant extracts on the spawning behavior of the plutella xylostella, so that the maximum protection of target crops is realized.

The invention relates to a pesticide composition containing a nicotine compound and application of the pesticide composition. The pesticide composition is prepared from flufenican amide, acetamiprid, thiamethoxam or clothianidin and the like. The pesticide composition has an obvious synergistic effect, the usage amount of pesticides is reduced, the residual amount of the pesticides on crops is reduced, and the environmental friendliness is enhanced. The pesticide composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae.

The application relates to the technical field of pesticide chemistry, and discloses a phenyl isoxazole derivative containing a triazole ring as well as a preparation method and application thereof. The phenyl isoxazole derivative containing the triazole ring has the structural formula: The phenyl isoxazole derivative containing the triazole ring provided by the application has good insecticidal activity on agricultural pests such as Plutella xylostella, Spodoptera frugiperda, Helicoverpa armigera, Ostrinia nubilalis, and aphids when the concentration is 50 mu g / mL. Therefore, the phenyl isoxazole derivative containing the triazole ring provided by the application can be applied to the prevention and control of agricultural pests as an active pesticide ingredient.

The invention provides a pesticide composition containing antibiotic compounds, the pesticide composition is composed of a first effective component and a second effective component, the first effective component is a compound with a structural formula (I), the second effective component is methylamino abamectin benzoate, abamectin, spinosad, spinetoram or ivermectin, and the weight ratio of the first effective component to the second effective component is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention of agricultural and forestry pests. The invention verifies that the binary composition obtained by compounding the flufenutraniliprole and the antibiotic insecticide in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips and flea beetle. (I).

The invention relates to the technical field of pesticide chemistry, and discloses an isoxazoline derivative containing a triazole thioether structure and preparation and application of the isoxazoline derivative, three active fragments of isoxazole, triazole and thioether are innovatively subjected to structural splicing synthesis, and the preparation process is clear in step, mild in reaction condition, excellent in intermediate yield and easy to industrially amplify. The insecticidal activity of the derivative on major agricultural pests such as plutella xylostella, spodoptera frugiperda and ostrinia nubilalis reaches 100%, the activity of the derivative is equivalent to that of fluxazole amide, the derivative can prevent and treat various pests such as cotton bollworm, aphid and rice planthopper, and the derivative is not applied in the field, has no drug resistance of the pests, has the advantages of broad spectrum, high efficiency, difficult generation of drug resistance and the like, can be used for preparing insecticides, and has wide application prospects. A brand new solution is provided for green prevention and control of agricultural pests.

The invention relates to pyridino [1, 2-a] pyrimidine mesoion derivatives containing a pyrazole structure as well as a preparation method and application of the pyridino [1, 2-a] pyrimidine mesoion derivatives. The compound disclosed by the invention has a structure shown as a formula (I), has excellent insecticidal activity on spodoptera frugiperda, chilo suppressalis, plutella xylostella, ostrinia nubilalis, aphis aphis and the like, and can be used for preparing drugs or medicaments for preventing and treating pests such as spodoptera frugiperda, chilo suppressalis, plutella xylostella, ostrinia nubilalis, aphis aphis and the like. The structure and the preparation process are simple, and the production cost is low. (I).

The application provides an siRNA targeting a Plutella xylostella GST3 gene and an application thereof, a nucleotide sequence of a sense strand of the siRNA is shown in sequence 7, and a nucleotide sequence of an antisense strand is shown in sequence 8. The siRNA regulates the positive temperature effect of four chloro chlorantraniliprole on Plutella xylostella by reducing the expression of the GST3 gene, and further changes the sensitivity of Plutella xylostella to four chloro chlorantraniliprole, in particular, improves the control effect of the pesticide at low temperature. It is of great significance to efficiently control Plutella xylostella by using the temperature effect of the pesticide.

The invention provides a fused ring compound containing a triazole structure as well as a preparation method and application of the fused ring compound. The fused ring compound containing the triazole structure is a compound represented by a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The fused ring compound containing the triazole structure has high insecticidal and acaricidal activity, and especially has a very excellent killing effect on lepidoptera or coleoptera pests such as beet armyworms and plutella xylostella. Formula I.

The invention discloses an insect-resistant and antibacterial pyrrole alkaloid compound and a biosynthetic gene cluster thereof. The structure of the pyrrole alkaloid compound is shown as a formula (I), wherein compounds 2, 3, 4, 14 and 15 are new compounds. The gene cluster of the pyrrole alkaloid compound is disclosed for the first time, and each gene function is confirmed for the first time. PasA and PasD act together to form a pyrrolidone skeleton intermediate 8, and then the compound 8 is converted into a final product 1 by PasB. The invention discloses the inhibitory activity of the compounds on chitinase, lepidoptera pests, namely plutella xylostella and agricultural pathogenic fungi, and a biosynthetic pathway of a lead compound 1 for the first time. The fatality rate of the compound 1 on lepidoptera pest plutella xylostella reaches 100%, the effect of the compound 1 is better than that of DFB, and the compound 1 also has good inhibition potential on agricultural pathogenic fungi. The invention provides a new alternative compound for pesticide development, and lays a solid foundation for realizing green and efficient synthesis of the compound 1.

The invention discloses a novel insect short neuropeptide sNPF analogue and application thereof in pests, and relates to the technical field of agriculture. The invention provides a structural general formula of the novel insect short neuropeptide sNPF analogue, and the novel insect short neuropeptide sNPF analogue is applied to prevention and control of aphids, thrips, plutella xylostella and spodoptera frugiperda. According to the novel insect short neuropeptide sNPF analogue disclosed by the invention, terminal tyrosine Y, arginine R or leucine L residues are substituted and modified by hydrophilic, hydrophobic and steric hindrance natural amino acid or non-natural amino acid and other groups of a lead compound, and the obtained novel insect short neuropeptide sNPF analogue can be used for preventing and treating various pests.

The invention discloses a benzimidazole salt compound with insecticidal activity as well as a preparation method and application thereof, and aims to solve the problem that an existing insecticide cannot give consideration to insecticidal activity and resistance at the same time. The salt compound containing the benzimidazole structure provided by the invention has good insecticidal activity on aedes aegypti, mythimna orientalis and plutella xylostella, has no cross resistance or obvious negative cross resistance on aedes aegypti pyrethroid-resistant strains, and has the control effect on the plutella xylostella equivalent to that of commercial insecticide permethrin sold in the market. A lead compound with application potential and an innovative research and development thought are provided for creating novel resistance treatment pesticides with negative cross resistance characteristics.