141 results about "Panonychus citri" patented technology

The invention relates to a paecilomyceslilacinus strain having strong pathogenicity for diaphorina citri and belongs to the technical field of the microorganism. The strain is paecilomyceslilacinus ZJPL08 which is registered and conserved in China General Microbiological Culture Collection Center on March 15, 2013, and the conservation number of the strain is CGMCC No. 7318. The paecilomyceslilacinus ZJPL08 provided by the invention has stronger pathogenicity for diaphorina citri and can be developed into a biocontrol agent for biological prevention and treatment of diaphorina citri; and compared with the present chemical agent for preventing and treating diaphorina citri, the paecilomyceslilacinus ZJPL08 has the characteristics of high efficiency, low toxicity, low residue, no pollution and not easy creation of resistance to drugs. The strain ZJPL08 has stronger acting effect on diaphorina citri than other paecilomyceslilacinus strains; the strain ZJPL08 is quick to cultivation, high in sporulation quantity, high in germination rate of spores and easy for preparation, and has excellent market application prospect.

The invention provides an acarid-killing agent, wherein the agent comprises biomass gasified asphalt 5-90% by weight. The agent has good effect in eradicating pest such as Panonychus citri Mcgregor.

The invention discloses a pesticide composite containing etoxazole, comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from etoxazole; the active ingredient B is selected from any of the following compounds: azocyclotin, fenbutatin oxide and diafenthiuron, wherein the weight ratio of the active ingredient A to the active ingredient B is (1-50):(1-80). The composite disclosed by the invention can be used for preventing and curing various insects, has obvious synergy, expands an insecticidal spectrum and has higher activity on cereal crops, fruit trees, flowers, cotton, tee trees, red spiders on vegetables, Panonychus citri, Tetranychus urticae Koch, Tetranychus urticae, Phyllocoptruta oleivora Ashmead, Acaphylla theae Watt, chrysanthemum mite, rose mite, aphids, aleyrodid, leafhopper, cabbage caterpillar, plutella xylostella and the like; pesticide use amount is reduced, the residual amount of the pesticide on the crop is lowered, environmental pollution is lightened, and the composite is safe to human and livestock and has good environmental compatibility and has small possibility for insects to generate resistance to drugs.

The invention discloses a new method for surveying population density of microarthropods such as panonychus citri mcgregor, predatory mites and diaphorina citri kuwayama. The new method comprises the following steps of: randomly drawing a citrus sample plant; placing adhesive plate boxes below tree crowns in the east, south, west and north directions of the tree crown of the citrus sample plant; knocking branches of the tree crowns above the positions where the adhesive plate boxes are arranged for many times to drop the microarthropods; adhering the microarthropods falling in the adhesive plate boxes to adhesive plates of the adhesive plate boxes; collecting the adhesive plate boxes to perform statistical survey, wherein the adhesive plate boxes comprise box bodies; a layer of plate corresponding to the size of the bottom surface is arranged on the bottom surface of each box body; an adhesive material is coated on the upper surface of the plate; and thus, the adhesive plates are constructed. Thus, by surveying with the method provided by the invention, the used time is less, the sample can be stored for a long time, and most importantly, the detection sensitivity to the microarthropods of red spiders, predatory mites and the like is greatly improved; and since the detected objects are adhered and cannot escape, the detection is more convenient, simpler and more accurate.

The invention discloses an insecticidal composition containing pyriminostrobin. The insecticidal composition is characterized in that the active ingredients of the insecticidal composition are pyriminostrobin and a biogenic insecticide abamectin or milbemycins; the weight ratio of pyriminostrobin to the biogenic insecticide is (2:1)-(50:1). The composition can be prepared into agriculturally allowable dosage forms such as suspension concentrates, wettable powder, water dispersible granules, dispersible oil suspension concentrates, microemulsion or emulsifiable concentrates. The insecticidal composition has the advantages that the insecticidal composition has reasonable components and good acaricidal effects; the activity and acaricidal effects of the insecticidal composition are not simple superposition of the activities of the components; compared with existing single preparations, the insecticidal composition has obvious synergistic effects besides obvious insecticidal effects, has good safety to crops and has obvious control effects on Panonychus citfi McGregor, Panonychus ulmi or Tetranychus cinnabarinnum Boisduval.

The invention discloses an insecticide prepared from active ingredients of ginkgo biloba testa. The insecticide mainly comprises two active ingredients including ginkgolic acid and tartaric acid whichare extracted from the ginkgo biloba testa. By limiting a dosage relationship between the two active ingredients of ginkgolic acid and tartaric acid and regulating and adjusting a pH value of a finally prepared insecticide solution, the prepared insecticide has a significantly enhanced killing effect on plant, fruit and vegetable pests, such as lipaphis erysimi, plutella xylostella, panonychus citri and the like. In addition, the invention also provides a method for preparing the insecticide from the active ingredients of the ginkgo biloba testa, high-purity selective extraction of high-efficiency insecticidal components including ginkgolic acid and tartaric acid from the ginkgo biloba testa can be achieved, and meanwhile protection of bioactivity of ginkgolic acid and tartaric acid in the production process is ensured, so that the highly effective and environmentally friendly organic insecticide is prepared. The method is simple in operation, raw materials are environmentally friendly and easy to obtain, and the formula for preparing a product is simple. In addition, the insecticide has high efficiency, low toxicity, no residues and no pollution and is more conducive to ecological balance protection.

The invention discloses an acaricidal composition containing pyriminostrobin and bifenazate as well as application of the acaricidal composition. The acaricidal composition consists of effective ingredients including first active ingredients of pyriminostrobin and second active ingredients of bifenazate, and the weight ratio of the first active ingredients to the second active ingredients is 1:10-5:1. The composition can be prepared into agriculturally allowed suspending agents, wettable powder, water dispersible granules or dispersible oil suspension agent dosage forms. The acaricidal composition has the advantages that the ingredients are reasonable, the insect killing effect is good, in addition, the activity and the insect killing effect are not the simple superposition of the activities of each ingredient, compared with the existing simple preparation, the acaricidal composition has the obvious insect killing effect and has the obvious synergistic effect, good safety is realized on crops, the safety requirement of pesticide preparations is met, and the acaricidal composition has the obvious preventing and curing effects on panonychus citri mcgregor, European red mite and cotton spider mites.

The invention discloses a Bifenazate-bearing insecticidal composition which comprise an active component A and an active component B. the active component A is selected from Bifenazate. The active component B is any one compound selected from a group consisting of mineral oil and flufenoxuron. And the weight ratio of the active component A to the active component B is 1:60-60:1. The composition provided by the invention can be used to control a plurality of insects, has obvious synergism, expands insecticidal spectrum, and has high activity to aphid, whitefly, trialeurodes vaporariorum, red spider, Brevipalpus obovatus, Panonychus citri Me Gregor, rust mite and the like on vegetables, fruit trees, cotton and paddy rice. In addition, pesticide dosage is reduced, residual quantity of a pesticide on a crop is decreased, and environmental pollution is mitigated. The Bifenazate-bearing insecticidal composition is safe to people and livestock and has good environment compatibility. By the adoption of the composition, insects are not easy to generate resistance.

The invention discloses application of a soy sauce residue glyceride in preparation of a preparation for controlling minisize insects or foliar fungal and bacterial diseases. The soy sauce residue glyceride is a glyceride containing C16-C22 fatty acids. According to the invention, the soy sauce residue glyceride is taken as a main component, and effective auxiliary agents are added for preparationof a pesticide preparation. Through tests, the pesticide preparation has a same controlling effect on tetranychus urticae, diaphorina citri, panonychus citri, aedes albopictus and other minisize insects and sooty mould and other diseases compared with a current mineral oil emulsion, and does not show phytotoxicity after repeated application to crops for a short period of time. The pesticide preparation has the advantages of simple and stable ingredients, high safety, good anti-insect and disease-preventing effects, and no phytotoxicity to crops. The main components and additives of the pesticide preparation are food grade, and easy to degrade in environment, and the pesticide preparation is easy for industrialized standardized production, and has strong market competitiveness compared with mineral oil emulsions.

The invention discloses a composition of insecticide and acaricide, which comprises active constituents A and B, the active constituent A is selected from diafenthiuron, the active constituent B is selected from propargite, azocyclotin or Fipronil, wherein the weight ratio of the two active component A and B is 1:99 to 99:1. The composition has synergic action and can be used for controlling multiple agricultural pest insects including Panonychus citri (MeGrgor), Eriophyes oleivora, spidermite on apple, Tetranychus urticae Koch and apple aphis.

An ethanolic extract of avocado seeds with a composition of 2-Furfuraldehyde (1,00%), 5-Methylundecane (1,05%), (11Z)-Pentadecenal (0,98%), (11Z)-Hexadecenal (2,35%), (3E)-Nonadecene (1,07%), 2-Tetradecylfuran (5,58%), 2-Pentadecylfuran (7,10%), 2-Hexadecylfuran (6,52%), 2-(1Z-Heptadecenyl) furan (2,85%), 2-(1E-Pentadecenyl) furan (2,15%), 2-(1E-Heptadecenyl) furan (5,60%), 2-(8Z, 11Z-Heptadecadienyl) furan (5,49%), 2-(8Z, 11 E-Heptadecadienyl) furan (33,53%), 2-(1 E, 8Z, 11Z-Heptadecatrienyl) furan (15,56%), and other unidentified compounds (9,17%), useful as miticide and insecticide is described.

The invention relates to an acaricide composite for agricultural use, in particular to an acaricide composite containing the first active ingredient of spirodiclofen and the second active ingredient of bifenazate. Compared with siple dosage, the acaricide composite has a remarkable synergistic effect on acarina, has good preventive and therapeutic effects on panonychus citri on fruit trees, cotton and vegetables, panonychus ulmi, tetranychus urticae, tetranychus cinnabarinus or carmine spider mite, can kill eggs, larva, nymphs and adults, and is favorable for delaying production of drug resistance of acarina.

This invention relates to the extraction and use of bioactive compounds from avocado seeds (var. Hass; Persea americana Mill) at room temperature. The extraction was performed with dichloromethane as a solvent using a simple diffusion mechanism within pressed avocado seeds. The extracted compounds were identified as furan derivative structures and fragments derived thereof that exhibited repellent and miticide activity against mite species of economic interest: Tetranychus urticae, Tetranychus cinnabarinus, Oligonychus yothersi, Panonychus citri, and Brevipalpus chilensis. The compounds obtained have the general structure of a disubstituted furan, as illustrated in FIG. 1, and the fragments thereof are derived from mono-substituted furans and from side-chain fragments. The utility of these compounds as repellents was determined over a 48-hour period, and their function as miticides was evaluated for 24 hours after application.

The invention discloses a method for RNA (ribonucleic acid) interference of panonychus citri mites. The method includes the steps of feeding the panonychus citri mites, treating the panonychus citri mites with dsRNA solution, determining the molting and survival situation of treated the panonychus citri mites, and evaluating the effect of the RNA interference. The method has novel and reasonable design, enables the panonychus citri mites not to complete the normal molting so as to die with RNA interference, and thus, the purpose of preventing the panonychus citri mites is achieved, the amount of the chemical pesticides to be used can be reduced, and accordingly the method has a broad application prospect.

The invention relates to a molecular marker method for rapidly detecting the pyrethroid resistance of panonychus citri. The method comprises the following steps: (1) preparing a PCR amplification template, wherein a single panonychus citri is adopted as a sample; (2) carrying out nested PCR amplification. In the first round of PCR, the sample prepared in the step 1 is adopted as an amplification template, and PCR amplification is carried out with primers A and B; and in the second round of PCR, the product of the first round of PCR is adopted as an amplification template, and PCR amplification is carried out with primers C and D; (3) carrying out enzyme digestion, wherein the product of the second round of PCR is subjected to an enzyme digestion reaction with BclI enzyme; and (4) carrying out electrophoresis, wherein the reaction liquid after enzyme digestion in the step 3 is subjected to electrophoresis with 1.5% agarose gel, and whether bands appear at 307bp and 281bp is observed. According to the method, a DNA extraction step is avoided, and single panonychus citri F1538I mutation is rapidly detected directly with nested PCR and enzyme digestion.

The invention belongs to the technical field of preventing and controlling of insects and pests, and particularly discloses a special sticking attractant for diaphorina citri. The special sticking attractant is prepared from the following raw materials in parts by weight: 8 to 12 parts of insect attracting agent, and 50 to 80 parts of insect sticker, wherein the insect attracting agent is preparedfrom the following raw materials in parts by weight: 2 to 4 parts of plant-sourced pheromone, 2 to 3 parts of insect pheromone, 10 to 15 parts of ethyl alcohol, 1 to 2 parts of chitosan, 0.8 to 2 parts of propolis, 8 to 15 parts of plant leaf extract, and 2 to 5 parts of konjac glucomannan. The special sticking attractant for diaphorina citri has the advantages that by utilizing the sensitivity of the diaphorina citri to light and terpene compounds, the raw materials are reasonably compounded, and the prepared sticking attractant has stronger killing ability on the diaphorina citri; by addingthe konjac glucomannan and the chitosan into the raw materials, the timeliness is longer, and the certain slow-release efficiency is realized.

The invention relates to a synergistic pesticide composition. The active ingredients of the composition are cyflumetofen and an organophosphorus insecticide, wherein the weight ratio of the cyflumetofen to the organophosphorus insecticide is 20:1-1:50, and the two active ingredients are prepared into various forms including missible oil, microemulsions, EW (emulsions in water), suspending agents, microcapsule suspending agents and suspoemulsions. The composition is used for preventing and controlling pest mites of crops, and especially can be used for preventing and controlling panonychus citri mcgregor and tetranychus urticae koch; and the composition has a synergistic effect, is quick-acting and long-acting, and can reduce consumption of pesticide, save manpower, and contribute to delaying the development of insect resistance.

The invention discloses a miticide composition, which contains two active components A and B, wherein the active component A is selected from tebufenpyrad, and the active component B is selected from clofentezine or flufenoxuron; the ration of A to B in portion by weight of the active components is between 1:1 and 1:50; and the weight percentage of the active components in the composition is between 1 and 99 percent. The composition has obvious synergism, can be applied to controlling various agricultural mites, particularly has prominent effect on controlling red spider mites, panonychus citri, and tetranychid mites.

The invention relates to a pesticide composition for preventing and treating drug-fast tetranychid mites, which is used for preventing and treating pest mites on crops, and especially can be used for preventing and treating panonychus citri, two-spotted spider mites and tetranychus cinnabarinus. The active components of the composition are cyflumetofen and etoxazole, wherein the mass ratio of the cyflumetofen to the etoxazole is 30:1-1:30, and the composition can be prepared into various forms including missible oil, microemulsions, EW (emulsions in water), suspending agents, wettable powder, water dispersible granules, microcapsule suspending agents and suspoemulsions.

The invention discloses a molecular detection method of panonychus citri Mc Gregor response abamectin stress protein encoding gene. The molecular detection method comprises the following steps: firstly, extracting total RNA (Ribonucleic Acid) of panonychus citri Mc Gregor treated by abamectin, and then transcribing the RNA into cDNA (complementary Deoxyribonucleic Acid); secondly, carrying out real-time quantitative detection on RPSA, EEF2, ATPsyn-alpha, FAH, V-ATPsyn-B, CALR, sHsp, CarE-B, Actin, CRY, Fer, Vg, ATPsyn-D, TPX, TCP-1-eta and GAPDH genes by using a qPCR (quantitative Polymerase Chain Reaction) method, wherein upstream and downstream sequences of qPCR detecting primer pairs of 16 genes are shown as SEQ ID No: 1 to 32; thirdly, analyzing qPCR results to obtain expression results of the 16 genes treated by the abamectin with different concentrations at different time periods.

The invention provides a preparation method of a mandarin orange peel composite plant agricultural insecticide. The method comprises the following steps: preparing waste mandarin orange peel as a raw material; extracting mandarin orange alkaloid with an organic solvent; adding capsaicin, sophocarpidine and rotenone, and compounding into a wide-spectrum plant agricultural insecticide. The insecticide can be used for effectively preventing and controlling tetranychus cinnabarinus, panonychus citri, vegetable aphids, cabbage caterpillars, rice-stem borers, euproctis pseudoconspersa strands and empoasca flavescens, and has stomach killing and touch killing effects as a wide-spectrum insecticide. The insecticide is a safe, efficient, residue-free and nuisanceless plant-derived pesticide. The insecticide replaces chemical agricultural insecticides, and is a plant agricultural insecticide with a development prospect.

The invention relates to an insecticidal composition containing flufenzine and spirotetramat. The insecticidal composition comprises a binary compound composed of flufenzine and spirotetramat as active ingredients, and the rest of auxiliary ingredients, wherein the mass ratio of the flufenzine to the spirotetramat is (32-48): (20-4); the total mass of the flufenzine and the spirotetramat as active ingredients in a preparation accounts for 1%-80% of the total mass of the whole preparation; and the insecticidal composition can be prepared into pesticide formulations, including emulsifiable concentrates (ECs), suspensions, wettable powders, water dispersible granules, emulsions in water (EW), and micro-emulsions. The insecticidal composition containing flufenzine and spirotetramat has good control effects to harmful mites, including panonychus citri mcgregor, phyllocoptruta oleivora ashmead, polyphagotarsonemus latus, tetranychus cinabarinus boisdu, tetranychus urticae and the like, as well as aphid, thrip, psyllidae, scale insect, and oystershell scale.

The invention relates to an acaricidal pesticide composition. The active compositions of the composition are cyflumetofen and bifenazate, wherein the weight ratio of the cyflumetofen to the bifenazate is 50:1-1:50, and the two active compositions are prepared into various forms including missible oil, microemulsions, EW (emulsions in water), suspending agents, wettable powder, water dispersible granules, microcapsule suspending agents, suspoemulsions, and oil miscible flowable. The composition is used for preventing and controlling pest mites of crops, and especially can be used for preventing and controlling panonychus citri mcgregor and tetranychus urticae koch; and the composition has a synergistic effect, is quick-acting and long-acting, and therefore pesticide consumption is reduced and labor is saved.

The invention discloses a pesticide composition containing etoxazole. The pesticide composition contains active ingredients, namely etoxazole and clofentezine, wherein the weight ratio of etoxazole to clofentezine is (1-50 percent):(1-80 percent). The pesticide composition can be used for preventing and controlling insect pests, has an obvious synergistic effect, has quite high activity in killing phytophagous mites, tetranychus urticae, eotetranychus kankitus, panonychus citri, four-spot yellow spiders, eriophyes oleivorus, european red mites, tetranychus viennensis zacher, pear red spiders, two-spotted spider mites and tetranychus cinnabarinus, alleviates environmental pollution, is safe for people and livestock, has high environmental compatibility, and avoids insect pest resistance to pesticides; an insecticide controlling spectrum is expanded; and the using amount of pesticides and pesticide residues on crops are reduced.

The invention discloses an acaricidal composition containing pyrenzafen and spirotetramat and the application thereof. The effective active ingredients are composed of the first active ingredient pyretafen and the second active ingredient spirotetramat. The weight ratio of the active ingredient to the second active ingredient is 50:1-1:50, and the sum of the contents is 1%-65% of the total weight of the composition. The composition can be formulated into agriculturally acceptable suspensions, microemulsions, water emulsions, dispersible oil suspensions, emulsifiable concentrates, wettable powders, and water-dispersible granules. The composition of the present invention is reasonable, and has good effect on apple claw mite, two-spotted spider mite, citrus red spider mite and cinnabarinus spider mite, and its acaricidal effect is not a simple superposition of the activities of each component. Compared with the existing single preparation, In addition to having a significant acaricidal effect, it also has a significant synergistic effect, reduces the dosage of the drug, and is safe for crops.

The invention discloses an acaricidal composition containing pyriminostrobin and propargite. Effective active ingredients of the acaricidal composition consist of pyriminostrobin and propargite, and the weight ratio of pyriminostrobin to propargite is (1: 10) to (5: 1). The composition disclosed by the invention can be prepared into agriculturally-acceptable preparation forms, such as emulsifiable concentrate and microemulsion. According to the acaricidal composition, the ingredients are reasonable, the acaricidal effect is good, and the activity and acaricidal effect of the acaricidal composition are not achieved through the simple superposition of the activity of each component; compared with the existing single preparations, besides having a remarkable acaricidal effect, the acaricidal composition has a remarkable synergism has good safety to crops and meets the requirements of pesticide preparations for safety; the acaricidal composition has remarkable prevention and treatment effects on Panonychus citri McGregor, Panonychus ulmi and Tetranychus cinnabarinnum Boisduval.

The invention discloses an application method of 2 chitinase genes. Two chitinase genes Unigene10731 and Unigene 7021 are identified from an orange panonychus citri me gregor transcriptome database and are subjected to authenticity testing; the two selected genes are compared on NCBI (National Center for Biotechnology Information) to find a conserved sequence; and the two genes are jointed into a multi-gene segment according to the multi-gene large-segment construction strategy, and the Unigene10731 and the Unigene 7021 are jointed together to form a multi-gene; a primer containing a restriction enzyme cutting site is designed, and forward and reverse segments of the multi-gene chitinase order are sequentially jointed into two terminals of the intron of the vector, so that an RNAi (ribonucleic acid interfere) interference vector 'PFGC+Ug10731 / Ug7021' with a reverse complementary hairpin structure can be formed, and the vector is reprinted into agrobacterium to infect an orange nursery stock.

The invention discloses an insecticidal composition containing tolfenpyrad. The insecticidal composition comprises an active component A and an active component B, wherein the active component A is selected from the tolfenpyrad, the component B is selected from anyone of propargite, clofentezine, and pyridaben, and a weight ratio of the active component A and the active component B is 1 : 70 to 70 : 1. The composition can prevent and control a plurality of insects, is obvious in synergy effect, capable of expanding insecticidal spectrum and relatively high in activities on rice hopper, aphid, Bemisia tabaci, Trialeurodes vaporariorum, Empoasca pirisuga Matumura, Tetranychus cinnbarinus, Panonychus citri, and Phyllocoptes on vegetables, fruit trees, cottons, and paddy rice; the composition reduces pesticide dosage and residual quantity of the pesticide on crops, alleviates environment pollution, and is safe to human and animals and good in environmental compatibility. Pests are not easy to have drug resistance.

The invention relates to an insect and mite killing composition containing Cyflumetofen and emamectin benzoate. The insect and mite killing composition is prepared by binary formulation of Cyflumetofen and emamectin benzoate which serve as active ingredients. The weight ratio of Cyflumetofen to emamectin benzoate is 1:5-40:1. The insect and mite killing composition also comprises auxiliary ingredients which are allowed and acceptable in pesticides. The insect and mite killing composition can be used for preventing and controlling insects and mites like polyphagotarsonemus latus, Panonychus citri Me Gregor, Tetranychus urticae, tetranychus cinnabarinus, tea lesser leafhopper, tea leaf roller, cavity small leaf moth, worms in appetite and so on. The insect and mite killing composition has an obviously improved effect and a continuous preventing and controlling effect, and is safe to human beings and livestock and has high environmental compatibility, and the dosage of pesticides is reduced.

The invention discloses an acaricidal composition containing pyriminostrobin and an organic tin acaricide. The acaricidal composition takes pyriminostrobin and the organic tin acaricide as active ingredients, wherein the weight ratio of pyriminostrobin to the organic tin acaricide is (1: 8)-(5: 1). The composition can be prepared into agriculturally acceptable dosage form such as suspension, wettable powder, water dispersible granules, a dispersible oil suspension or missible oil. The composition is reasonable in composition and good in acaricidal effect, and activity and acaricidal effect of the composition are not simple superposition of activities and acaricidal effects of various components; the composition, compared to an existing single preparation, not only has an obvious insecticidal efficacy but also is remarkable in synergistic effect, and is good in crop safety. The composition has obvious effect on preventing and controlling panonychus citri, panonychus ulmi and cotton red spider.