47 results about "Carboxylesterase" patented technology

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

The present invention relates to fusarium toxin cleavage polypeptide variants of a fusarium toxin carboxylesterase having SEQ ID No.1, 46 wherein said polypeptide variants each have an amino acid sequence shortened by 47 amino acids at the N-terminus and said amino acid sequence has a sequence identity of at least 70%, preferably 80%, particularly preferably 100%, to the amino acid sequence segments 48-540 of SEQ ID No.46, i.e. SEQ ID No.1; and isolated polynucleotides encoding the polypeptide variants; and an additive for cleaving fusarium toxins comprising at least one polypeptide variant and optionally at least one adjuvant; the invention also relates to the use of said polypeptide variants or additives for hydrolytically cleaving at least one fusarium toxin, and to a method for hydrolytically cleaving at least one fusarium toxin.

The present disclosure provides engineered carboxyesterase enzymes that have the ability to catalyze amide bond formation. Also provided are polynucleotides encoding the carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes to make commercially valuable amides. Also provided are amides that are made using the engineered carboxyesterase enzymes.

The invention belongs to the technical field of genetic engineering, and discloses application of an anchoring protein INP-N in a PET degrading enzyme system displayed on the surface. According to the invention, after efficient anchoring protein INP-N is screened, FAST-PETase is mediated by the efficient anchoring protein INP-N to construct recombinant escherichia coli of which the surface displays PET degrading enzyme. Furthermore, hydrophobin HFBII is introduced into the surface display system, so that the relative enzyme activity of the PET degrading enzyme is improved by 1.1 times or more. Meanwhile, FAST-PETase and carboxylesterase Est30KL are synchronously expressed, so that the proportion of TPA in a degradation product can reach 95% or above. In addition, the PET degrading enzyme surface display system provided by the invention is excellent in cycle performance, more than 65% of activity can still be retained when PET is cyclically degraded for the third time, and the application cost can be greatly reduced. The method can be applied to the fields of degradation of PET products, preparation of PET degradation agents or BHET degradation agents and the like.

The invention discloses a molecular marker for interaction resistance of panonychus citri to insecticides as well as application and a detection method of the molecular marker. According to the molecular marker for the cross resistance of the panonychus citri to the multiple medicaments, the molecular marker is carboxylesterase genes PcCCE11 and / or PcCCE7, the nucleotide sequence of the PcCCE11 gene is as shown in SEQ ID NO.1, and the nucleotide sequence of the PcCCE7 gene is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the molecular marker for the cross resistance of the panonychus citri to the multiple medicaments.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The present invention discloses a pyridine-based water-soluble dual-response fluorescent probe and a preparation method and application thereof, and relates to the technical field of fluorescent probes. The technical scheme is as follows: the pyridine-based water-soluble dual-response fluorescent probe has the following structure:. The present invention uses acetyl as the recognition site of carboxylesterase and terminal benzyl pinacol ester as the reaction site of hypochlorite to prepare an on-off and ratio fluorescent probe that can simultaneously respond to hypochlorite and carboxylesterase; in addition, the pyridinium salt provides an effective mitochondrial targeting site, which enables the probe to detect mitochondrial hypochlorite and carboxylesterase in living cells.

The present invention belongs to the field of biomedicine, and specifically relates to a diagnostic and therapeutic integrated probe responsive to carboxylesterase, and a preparation method and application thereof. The present invention discloses a small molecule diagnostic and therapeutic probe represented by the following structural general formula. This small molecule diagnostic and therapeutic probe is obtained by reacting a near-infrared fluorescent dye, chlorambucil, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), and 4-dimethylaminopyridine (DMAP) in an organic solvent. The present invention encapsulates the above small molecule diagnostic and therapeutic probe with poly(lactic acid-co-glycolic acid)-polyethylene glycol (PLGA-PEG) to obtain a nano diagnostic and therapeutic integrated probe. The fluorescence wavelength of the obtained nano diagnostic and therapeutic integrated probe is located in the near-infrared region, which can realize near-infrared fluorescence imaging diagnosis of tumors, and has combined therapeutic properties of chemotherapy, photodynamic therapy, and sonodynamic therapy, and can significantly kill tumor cells.

The invention relates to an application of carboxylesterase TfdI in degradation of phenoxy carboxylic acid herbicides. The invention provides application of rhodococcus pyridinivorans Y6 in degrading phenoxy carboxylic acid herbicides, a novel phenoxy carboxylic acid herbicide degrading enzyme TfdI is cloned from the rhodococcus pyridinivorans Y6, the rhodococcus pyridinivorans Y6 has excellent catalytic action on phenoxy carboxylic acid herbicides, the degradation rate on 2, 4-D isooctyl ester with the concentration of 100 mg / L within 1 h is 100%, and the degradation effect is stable. The excellent degrading enzyme provided by the invention has good application potential in bioremediation of the environment polluted by the phenoxy carboxylic acid herbicide, a new scheme is provided for solving the problem of residual pollution of the 2, 4-D isooctyl ester herbicide in agricultural production, and meanwhile, a herbicide degradation gene resource library is enriched.

Methods of treating a metabolic disorder in a subject are provided. Aspects of the method include enhancing extracellular Carboxylesterase 2 (CES2) activity in the subject in order to treat the subject for the metabolic disorder. Also provided are compositions for use in practicing the methods.

The invention discloses a pH / esterase dual-response nano pesticide delivery system as well as a preparation method and application thereof. The nano pesticide delivery system comprises a carboxymethyl chitosan-polycaprolactone graft copolymer (CMCS-g-PCL) carrier, and a pyrethroid pesticide which is loaded on the carrier through physical embedding. The nano pesticide delivery system has good storage stability and foliar affinity, and the photolysis resistance and enzymolysis resistance of the loaded pesticide are remarkably improved. After application, the system shows excellent environmental responsiveness, keeps stable deposition under an acidic condition, and accurately triggers pesticide release in alkaline intestinal tracts of lepidoptera pests and in a carboxylesterase environment. The method is suitable for precise prevention and control of lepidoptera pests, and has the effects of pesticide reduction and environmental risk regulation and control.

The application discloses an esterase whole cell catalyst and preparation and application thereof, the esterase whole cell catalyst can express a carboxylic acid esterase mutant disclosed in the application, the carboxylic acid esterase mutant is subjected to the following mutations on the amino acid sequence shown in SEQ ID NO. 1: the glycine at the 236th position is replaced by valine, the arginine at the 417th position is replaced by phenylalanine, and the threonine at the 545th position is replaced by alanine. The application effectively solves the problems of low catalytic activity of a warming catalyst for resolving methyl chrysanthemate and poor product tolerance in the prior art. The esterase whole cell catalyst of the application significantly improves the activity of selectively resolving methyl chrysanthemate to prepare dextro-trans chrysanthemic acid, and the optical purity can reach more than 99%. Moreover, the carboxylic acid esterase mutant disclosed in the application can be expressed in a soluble form in cell supernatant, can be produced in a large scale through microbial fermentation, has mild reaction conditions, and is simple to operate, and is more suitable for industrial production.

The application discloses a carboxylic esterase lyophilization protective agent, a preparation method and application thereof, and belongs to the technical field of bioengineering. The carboxylic esterase lyophilization protective agent is a single-component polyvinylpyrrolidone; wherein, the polyvinylpyrrolidone has a viscosity-average molecular weight Mv of 10000-15000000; a K value of 17-90; when used for lyophilization protection of carboxylic esterase, the carboxylic esterase lyophilized powder can effectively improve the stability of the carboxylic esterase lyophilized powder at normal temperature, prevent the purity and activity of the carboxylic esterase lyophilized powder from decreasing, and the lyophilized powder cake is complete, easy to be powdered, the enzyme powder resuspension is clear and transparent, the enzyme activity recovery rate is high, and the carboxylic esterase is convenient for being applied to different application scenarios.

The present invention discloses a fluorescence probe for two - photon detection CE, its preparation, screening and application, belonging to the technical field of synthesis and detection. The structural formula of the fluorescence probe is as follows: #imgabs0# wherein, R1 is H, R2 is H; or, R1 is H, R2 is F; or, R1 is F, R2 is H. The fluorescence probe is synthesized in one step by reacting 7 - amino - 4 - methylcoumarin with differently substituted chloroacetyl chloride and triethylamine to obtain the fluorescence probe P. By comparing the fluorescence response multiples, response times and stabilities of the three fluorescence probes, the fluorescence probe P2 with R1 being H and R2 being F is selected for subsequent testing and application. The fluorescence probe P2 can react with CE to break the amide bond, and the ICT effect disappears, resulting in enhanced fluorescence. Therefore, the fluorescence probe P2 can be used to detect CE, and the probe has a simple structure, and has the advantages of high selectivity, high sensitivity and rapid response, etc.

This invention discloses a carboxylesterase-activated mitochondrial-targeting fluorescent probe with photodynamic therapy properties, its preparation method, and its uses. The structure of the mitochondrial-targeting fluorescent probe is shown below. The mitochondrial-targeting fluorescent probe CML of this invention can specifically respond to different concentrations of carboxylesterases (CEs), with a detection limit as low as 0.30 U / L. Simultaneously, this probe can specifically target mitochondria, and through structural optimization (replacing the methyl group on the indole salt with a butyl group), its mitochondrial anchoring ability is significantly superior to that of methyl-substituted derivatives. Cytotoxicity experiments confirmed that the probe has good biocompatibility; confocal fluorescence imaging experiments showed that this probe can effectively monitor the fluorescence changes of endogenous and exogenous CEs in HepG2 cells. Most importantly, after being specifically activated by carboxylesterases, the probe can efficiently generate reactive oxygen species under light conditions, thereby exerting a photodynamic therapeutic effect on cancer cells.

The invention belongs to the field of biological enzyme catalysis, and provides a carboxylesterase CarEst3 mutant, the mutant is obtained by mutating phenylalanine at the 66th site of an amino acid sequence as shown in SEQ ID NO.2 into arginine, and the mutant can be used for catalyzing kinetic resolution of racemization 3-cyclohexene-1-methyl formate to prepare (S)-3-cyclohexene-1-formic acid. High stereoselectivity is achieved, and large-scale production can be achieved; the method for preparing (S)-3-cyclohexene-1-formic acid has the advantages of mild reaction conditions, high substrate concentration, good stereoselectivity, simple post-treatment, high product yield and the like.

The application relates to a synthesis method of a compound of formula I, a genetically engineered bacterium for synthesizing terpenes and application of the genetically engineered bacterium, relates to the field of biosynthesis, and a synthesis method of terpenes, and comprises the following method: a compound of formula II is sequentially subjected to the action of cytochrome oxidase, carboxylic acid esterase and cyclase to generate the terpene compound shown in formula I. The application solves the problem of multiple synthesis steps of the compound shown in formula I. The application solves the problem of low enzyme rate of each step through gene optimization.

The invention provides a biosynthesis method of an esdoxaban tosylate chiral intermediate. According to the preparation method, 3-cyclohexene-1-carboxylic acid methyl ester is taken as a starting raw material, and a target intermediate is finally obtained through carboxylesterase resolution, hydrolysis, hydroxylation, dehydrogenation oxidation, transamination reaction, amidation and Boc2O reaction in sequence. According to the method, high-selectivity carboxylesterase is used for realizing chiral resolution; p450 oxidase with excellent regioselectivity and stereoselectivity is utilized to realize specific hydroxylation of a C-H bond under a mild condition, and a traditional process depending on a highly toxic chemical oxidant is overturned; then, clean oxidation is realized through alcohol dehydrogenase, and a way is laid for subsequent reaction; most importantly, the transaminase is adopted to directly construct the chiral amine, so that the use of a high-risk reagent sodium azide is thoroughly avoided, and major potential safety hazards are eliminated from the source; and finally, green amidation is realized by virtue of lipase, and an acyl chloride method for generating corrosive wastewater is replaced.

This invention discloses an alkaloid, its preparation method, and its application in the preparation of human carboxylesterase 2 inhibitors. The residue of Paeonia lactiflora after ultrasonic extraction was extracted with 95% ethanol and then with ethyl acetate to obtain ethyl acetate extract CS-5. Separation was performed by silica gel column chromatography, using a gradient elution with petroleum ether-acetone mixed solvent and dichloromethane-methanol mixed solvent to obtain six eluent fractions, named A-F. Compound D was separated by silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-preparative HPLC to obtain compound I-2. Compound F was separated by Sephadex LH-20 column chromatography and semi-preparative HPLC to obtain compounds I-1 and I-3. Compound I-1 was separated by a chiral HPLC column to obtain enantiomers I-(+)-1 and I-(–)-1. The four alkaloids of this invention have a pH range of 13.65–14.16. m At concentrations of M, it can significantly inhibit the activity of human carboxylesterase 2. Therefore, it can be applied to the preparation of attenuated and protective agents for anticancer drugs such as irinotecan.

The invention belongs to the technical field of medicinal chemistry, and discloses a human carboxylesterase 2A (hCES2A) self-activated covalent inhibitor as well as a preparation method and application thereof. The inhibitor is a novel benzamide-O-carbamate derivative (I) with a structure as shown in a formula (I) and pharmaceutically acceptable salts thereof, and the compound can powerfully and highly selectively inactivate the activity of human intestinal tract hCES2A. Further, the oral bioavailability of the oral prodrug is improved or the intestinal toxicity caused by the carboxylesterase 2A activated prodrug (such as irinotecan) is relieved. The IC50 value of the compound for inhibiting human carboxylesterase 2A can reach a nanomole level and is dose-dependent and time-dependent. The compound provided by the invention also has good safety, and has the advantages of simple preparation process, high yield and the like, which indicates that the compound has good application prospects.

The present disclosure provides engineered carboxylesterase enzymes having improved activity in catalyzing cyclization of short polypeptides, as compared to a naturally occurring wild-type carboxylesterase enzyme. These variants catalyze the cyclization of linear tetrapeptides with high regioselectivity. Also provided are polynucleotides and expression vectors encoding the carboxylesterase enzymes and methods of using the carboxylesterase enzymes to generate cyclic tetrapeptides containing non-canonical amino acids.

The invention discloses a quality detection and analysis method of fen-flavor yeast for making hard liquor. The quality detection analysis method comprises the following steps: providing a to-be-detected sample of the fen-flavor yeast for making hard liquor, and preparing a crude enzyme solution of the to-be-detected sample; detecting the specific activity of carboxylesterase of the crude enzyme liquid of the sample to be detected; and performing quality evaluation according to the test result of the specific activity of carboxylesterase. The method can quickly evaluate the quality of three types of fen-flavor yeast based on determination of the specific activity of carboxylesterase in the yeast, and can be suitable for quickly evaluating the quality of a large-scale fen-flavor yeast sample.

The present invention belongs to the technical field of the preparation of biological compounds, and discloses a carboxylesterase fluorescent probe, a preparation method thereof and an application thereof. The preparation method includes: adding BDP, compound 1 and toluene into a Schlenk flask, and obtaining a mixed solution after complete dissolution; heating the mixed solution at 80-120 °C for 10-16 hours; after the reaction is completed, cooling the mixed solution to room temperature, adding dichloromethane to the mixed solution, filtering the precipitated solid under reduced pressure, and drying it in vacuum, thus obtaining the carboxylesterase fluorescent probe. The preparation method of the carboxylesterase fluorescent probe of the present invention is simple, has a high yield and low cost, and is conducive to industrial production; the carboxylesterase fluorescent probe prepared by the present invention has a 656-fold enhancement in fluorescence after reacting with carboxylesterase, and has an extremely high signal-to-noise ratio; the carboxylesterase fluorescent probe prepared by the present invention changes the solution color from red to yellow after reacting with carboxylesterase, and the increase in the concentration of carboxylesterase can be detected with the naked eye.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to cotton bollworm carboxylesterase HaCHE1alpha as well as a preparation method and application thereof. The coding gene sequence of the cotton bollworm carboxylesterase HaCHE1alpha is as shown in SEQ ID NO. 1. The cotton bollworm carboxylesterase HaCHE1alpha can efficiently degrade fumonisin B1, the action mode is mild, and secondary pollution is avoided. The cotton bollworm carboxylesterase HaCHE1alpha has a wide application prospect in the fields of environmental protection, food safety, animal health and the like. The preparation method provided by the invention provides a core technology for industrial production of the fumonisins B1 degrading enzyme.

The invention provides a carboxylesterase 2A covalent inhibitor as well as a preparation method and application thereof. The carboxylesterase 2A covalent inhibitor has a structure as shown in a formula I or a formula II. The carboxylesterase 2A covalent inhibitor provided by the invention can be specifically and covalently combined with serine residues in a mammalian carboxylesterase 2A catalytic center, so that the CES2A activity is highly selectively inhibited, and the carboxylesterase 2A covalent inhibitor shows a weak inhibition effect or no inhibition effect on other serine hydrolases. The half inhibitory concentration IC50 of the inhibitor on mammalian CES2A can reach a nano-friction level, and the inhibitor has good cell membrane permeability and metabolic stability. The inhibitor can effectively reduce intestinal toxicity caused by carboxylesterase 2A substrate drugs, and also can improve oral bioavailability of part of ester prodrugs.

The present invention provides a near-infrared fluorescent probe for detecting carboxylesterase and exhibiting antibacterial activity, as well as its preparation method and application. The probe has the following structural formula: #imgabs0#, wherein R is selected from #imgabs1#, #imgabs2#, #imgabs3#, #imgabs4#, #imgabs5#, or #imgabs6#. The probe of the present invention utilizes a hemicyanine derivative as a parent nucleus, introduces p-aminobenzenesulfonamide as an antibacterial active group, and utilizes acid esters containing different R groups as recognition groups for detecting carboxylesterase. Carboxylesterase-induced ester cleavage is accompanied by a significant enhancement in the fluorescence signal. The probe can effectively detect carboxylesterase from different proteases, amino acids, and yin and yang samples, exhibiting good selectivity and high sensitivity. The probe's action on carboxylesterase is accompanied by a distinct color change visible to the naked eye, and the probe exhibits a certain degree of antibacterial activity, resulting in successful applications for the detection and imaging of carboxylesterase in biological systems.

The invention relates to euphausia superba-sourced carboxylesterase, a coding gene and application thereof, and belongs to the field of enzymology, the amino acid sequence of the carboxylesterase is shown as SEQ ID NO.1, and the amino acid sequence of the gene for coding the carboxylesterase is shown as SEQ ID NO.2 or SEQ ID NO.3. The carboxylesterase can degrade a plasticizer PAEs, and the carboxylesterase can be used for degrading the plasticizer PAEs. And the plasticizer is at least one of dimethyl phthalate, diethyl phthalate, dipropyl phthalate and dibutyl phthalate, and has a very good application prospect.

The application discloses a carboxyesterase mutant and application thereof in production of optically pure (R)-3-cyclohexene-1-carboxylic acid, wherein the carboxyesterase EstA1 mutant is obtained by single-point mutation or combined mutation of positions 70, 128, 133, 227, 248, 249, 297, 301, 326 or 329 of the amino acid sequence shown in SEQ ID No. 1. The application provides the carboxyesterase EstA1 mutant with high catalytic activity and high stereoselectivity to racemic (R,S)-3-cyclohexene-1-carboxylic acid methyl ester, and the obtained mutant has the characteristics of mild reaction condition, high enantiomer selectivity, short reaction time, small amount of catalyst, simple reaction system and environmental friendliness in the process of enantioselective resolution of (R)-3-cyclohexene-1-carboxylic acid.

The invention belongs to the technical field of artificial mimic enzymes, and discloses a carboxylesterase simulant with phthalate degradation activity as well as a preparation method and application of the carboxylesterase simulant. The stimulant is Zn (II) (at) IMD PDA: beta-CD, and the structure of the stimulant takes beta-cyclodextrin as a skeleton, is bridged with an imidazole group through 1, 3-propane diamine, and is coordinated with Zn (II) ions to form a catalytic center. According to the design, a hydrophobic cavity of beta-cyclodextrin and a Zn (II)-imidazole active center are integrated into a whole, so that efficient recognition and catalytic hydrolysis of phthalate pollutants are realized. According to the preparation method, beta-cyclodextrin is taken as an initial raw material, one-pot three-step continuous reaction synthesis is carried out, the process is simple and convenient, the condition is mild, the cost is low, and the use of precious metals and tedious separation and purification steps are avoided. The simulant shows high-efficiency degradation capacity to various phthalic acid esters (such as DEHP, DBP and the like) under mild conditions, the degradation rate to the DEHP within 72 hours can reach 81.4%, and the activity of the simulant is still kept to be 98% or above after the simulant is recycled for 5 times.

The application discloses a lysosome-targeting near-infrared fluorescent probe and a preparation method and application thereof in carboxylesterase detection, wherein the structure of the lysosome-targeting near-infrared fluorescent probe is shown in the following formula: The lysosome-targeting near-infrared fluorescent probe of the application shows a rapid response (response time is 40 seconds) to carboxylesterase (CE) in an in-vitro experiment. Cell toxicity test shows that the probe has low biological toxicity, and confocal fluorescence microscopic imaging experiment shows that the fluorescent probe has good light stability in HeLa cells and can effectively target lysosomes (localization coefficient is 0.86), and is suitable for CE near-infrared region fluorescence imaging and detection in lysosomes of living cells.